FastQCFastQC Report
Sun 1 Jan 2017
ERR1142713_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142713_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659838
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23150.3508436919365056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20610.31234939485146357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16050.243241522919262No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23150.042.417911
GTATCAA33150.037.5214041
ATCAACG42200.029.3203833
TATCAAC43300.028.9876732
TCAACGC42900.028.7032994
CAACGCA43100.028.5701035
AACGCAG45650.027.1003886
ACGCAGA50600.024.5668057
CGCAGAG51950.024.0428928
CATGGGG23700.023.8448184
GCAGAGT56000.022.3040739
ATGGGGG12650.021.631475
GTACATG46800.020.9823631
ACATGGG44950.020.777283
ATTTAGG2306.1205355E-620.7003861
TACATGG47600.020.4953652
CAGAGTA56300.020.1779910-11
AGAGTAC54300.020.04491410-11
ATTACTC1500.004709969319.828853
GAGTACT24800.019.30621512-13
Produced by FastQC (version 0.11.5)