##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142713_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	659838
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.737099106144235	33.0	33.0	33.0	27.0	33.0
2	29.991763129737905	33.0	27.0	33.0	27.0	33.0
3	31.190987787911578	33.0	33.0	33.0	27.0	33.0
4	31.97245081368457	33.0	33.0	33.0	27.0	33.0
5	32.115128258754424	33.0	33.0	33.0	33.0	33.0
6	35.34107614293205	37.0	37.0	37.0	33.0	37.0
7	35.8940497516057	37.0	37.0	37.0	33.0	37.0
8	36.0385488559313	37.0	37.0	37.0	33.0	37.0
9	36.08924311724999	37.0	37.0	37.0	37.0	37.0
10-11	36.15677484473461	37.0	37.0	37.0	37.0	37.0
12-13	36.17993204392593	37.0	37.0	37.0	37.0	37.0
14-15	36.206208190495246	37.0	37.0	37.0	37.0	37.0
16-17	36.196040543284866	37.0	37.0	37.0	37.0	37.0
18-19	36.19298979446471	37.0	37.0	37.0	37.0	37.0
20-21	36.19708170793437	37.0	37.0	37.0	37.0	37.0
22-23	36.195392656985504	37.0	37.0	37.0	37.0	37.0
24-25	36.177865627623746	37.0	37.0	37.0	37.0	37.0
26-27	36.066091980152706	37.0	37.0	37.0	37.0	37.0
28-29	36.034718370266646	37.0	37.0	37.0	37.0	37.0
30-31	36.0524234736405	37.0	37.0	37.0	37.0	37.0
32-33	36.01027146057062	37.0	37.0	37.0	37.0	37.0
34-35	35.95051664196363	37.0	37.0	37.0	37.0	37.0
36-37	35.88741403192905	37.0	37.0	37.0	33.0	37.0
38-39	35.83775335764233	37.0	37.0	37.0	33.0	37.0
40-41	35.75761020129183	37.0	37.0	37.0	33.0	37.0
42-43	35.692163834153234	37.0	37.0	37.0	33.0	37.0
44-45	35.61670288767849	37.0	37.0	37.0	33.0	37.0
46-47	35.506179547100956	37.0	37.0	37.0	33.0	37.0
48-49	35.40462659016304	37.0	37.0	37.0	33.0	37.0
50-51	35.33496858319769	37.0	37.0	37.0	33.0	37.0
52-53	35.276930852724455	37.0	37.0	37.0	33.0	37.0
54-55	35.213169747725956	37.0	37.0	37.0	33.0	37.0
56-57	35.18080195441912	37.0	37.0	37.0	33.0	37.0
58-59	35.13539687014085	37.0	37.0	37.0	33.0	37.0
60-61	35.10301089055193	37.0	37.0	37.0	33.0	37.0
62-63	35.082197448464626	37.0	37.0	37.0	33.0	37.0
64-65	35.06298970353329	37.0	37.0	37.0	33.0	37.0
66-67	35.04021820507458	37.0	37.0	37.0	33.0	37.0
68-69	35.0035160145369	37.0	37.0	37.0	30.0	37.0
70-71	34.95280280917437	37.0	37.0	37.0	27.0	37.0
72-73	34.93024121072142	37.0	37.0	37.0	27.0	37.0
74-75	34.84279398882756	37.0	37.0	37.0	27.0	37.0
76-77	34.784208396606445	37.0	37.0	37.0	27.0	37.0
78-79	34.772382766679094	37.0	37.0	37.0	27.0	37.0
80-81	34.72883647198252	37.0	37.0	37.0	27.0	37.0
82-83	34.694994832064836	37.0	37.0	37.0	27.0	37.0
84-85	34.59824229583625	37.0	37.0	37.0	27.0	37.0
86-87	34.48601550683652	37.0	37.0	37.0	27.0	37.0
88-89	34.42937130022824	37.0	37.0	37.0	27.0	37.0
90-91	34.38772168320103	37.0	37.0	37.0	27.0	37.0
92-93	34.28799720537465	37.0	37.0	37.0	27.0	37.0
94-95	34.306381263279775	37.0	37.0	37.0	27.0	37.0
96-97	34.300917043274254	37.0	37.0	37.0	27.0	37.0
98-99	34.24282323843125	37.0	37.0	37.0	27.0	37.0
100-101	34.1616086069611	37.0	37.0	37.0	27.0	37.0
102-103	34.07030968207348	37.0	37.0	37.0	27.0	37.0
104-105	33.976555609104054	37.0	37.0	37.0	22.0	37.0
106-107	33.87939994362252	37.0	37.0	37.0	22.0	37.0
108-109	33.77042622583119	37.0	33.0	37.0	22.0	37.0
110-111	33.72077237140025	37.0	33.0	37.0	22.0	37.0
112-113	33.644652929961595	37.0	33.0	37.0	22.0	37.0
114-115	33.5563661080447	37.0	33.0	37.0	22.0	37.0
116-117	33.461255641536255	37.0	33.0	37.0	22.0	37.0
118-119	33.36291559443379	37.0	33.0	37.0	22.0	37.0
120-121	33.27477274725008	37.0	33.0	37.0	22.0	37.0
122-123	33.15663314328668	37.0	33.0	37.0	14.0	37.0
124-125	31.88902427565554	37.0	30.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	25.0
16	8.0
17	13.0
18	48.0
19	255.0
20	1114.0
21	2927.0
22	4742.0
23	6474.0
24	6022.0
25	4747.0
26	4792.0
27	5514.0
28	7140.0
29	9370.0
30	12269.0
31	16559.0
32	23516.0
33	34994.0
34	56979.0
35	115611.0
36	346719.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.57862498199543	19.378671983382738	12.50327872580755	24.539424308814276
2	17.47535001015401	22.908047126718984	35.0569382181687	24.559664644958307
3	21.45966131080659	25.4610980270915	28.314374134257196	24.764866527844713
4	15.86383848058754	17.421199877544957	33.963087685690645	32.75187395617686
5	16.361404333032745	35.88748361283087	31.398498063851232	16.352613990285157
6	30.896850860296045	34.36198056775144	18.19693224477177	16.544236327180748
7	26.87993113461183	31.272069811074836	22.469454623710668	19.378544430602663
8	26.41421076082311	31.80886823735523	21.300379790190927	20.47654121163073
9	26.853864130286524	15.759322742855064	20.822838333045386	36.563974793813024
10-11	25.814821213691847	24.966052273436816	26.79006059062982	22.42906592224152
12-13	26.501656467193463	23.674220035826977	26.890842903864282	22.933280593115278
14-15	24.723644288446557	24.242465574883532	25.27681036860563	25.757079768064283
16-17	24.00066379929619	26.125276204159203	26.05798696043574	23.81607303610886
18-19	23.9196590678318	25.076609713293262	28.101064200606817	22.90266701826812
20-21	24.96766229662483	24.129799411518206	27.91825513557587	22.984283156281094
22-23	24.653096669182435	23.97982535107102	27.93716033329393	23.429917646452616
24-25	24.043931995429183	24.595430999730237	27.835165601253642	23.52547140358694
26-27	23.822514011014825	24.68855991925291	27.940949142062145	23.547976927670124
28-29	23.685965343008437	24.65332399770853	28.202376947068824	23.45833371221421
30-31	24.10083990312774	24.387955831582904	28.15297088073133	23.358233384558027
32-33	23.68003285662433	24.559493147166002	27.99504119581139	23.76543280039828
34-35	23.952845858667914	24.60611424657036	28.119487266532893	23.321552628228833
36-37	23.9588101033309	24.67210853836338	27.7727174904747	23.596363867831023
38-39	23.980394846220936	24.483254310034532	27.716831621645994	23.81951922209854
40-41	23.680601023328	24.76197621126146	27.924790242009795	23.632632523400744
42-43	24.02015982977356	24.611451183416257	27.7148404051365	23.653548581673682
44-45	23.811125938016463	24.860401023586903	27.409517357709067	23.918955680687564
46-47	23.78427940318413	24.716936809960067	27.404426864291832	24.094356922563975
48-49	23.948605566820945	25.02015646264689	27.168789915100373	23.862448055431788
50-51	23.72612671595149	25.191865276022295	27.083844822517044	23.99816318550917
52-53	23.857313353666623	24.981377990793188	26.98603822910944	24.17527042643075
54-55	23.733812240939024	24.864549470701007	27.155273666901575	24.246364621458394
56-57	23.89791130550226	24.834277504478372	26.77452647468015	24.493284715339218
58-59	23.878838305076012	24.894404459337192	26.953498557959715	24.273258677627084
60-61	23.87095307556785	24.93629113228636	27.197529694811223	23.99522609733457
62-63	23.526608046217405	25.01492790654676	27.196448219108326	24.26201582812751
64-65	23.543751302404	24.674332695550042	27.359463504271886	24.42245249777407
66-67	23.714756394013058	24.70075897647294	27.30466358307216	24.279821046441842
68-69	23.757649604903023	24.589899338928646	27.196220890582236	24.4562301655861
70-71	23.476835594784692	24.652977219786397	27.511908424480964	24.358278760947947
72-73	23.67455901987915	24.86917223496106	27.3784283088096	24.07784043635019
74-75	23.565994110871227	24.680575809072717	27.60019140047668	24.153238679579374
76-77	23.607115069320056	24.63489617537724	27.458128138588545	24.299860616714163
78-79	23.58148423753499	24.746941243689633	27.509256214128857	24.162318304646522
80-81	23.34353280653736	24.835035266232016	27.59601599180405	24.225415935426575
82-83	23.4863169508556	24.855228081129866	27.517879578255645	24.140575389758894
84-85	23.426594375518086	24.89812727094847	27.24387341616631	24.431404937367137
86-87	23.184264472946214	24.974214069896377	27.410880106619796	24.430641350537616
88-89	23.35063842580853	24.633260094173313	27.414576668038322	24.601524811979843
90-91	23.764139047010982	24.61568559354614	27.163349072663536	24.456826286779343
92-93	23.54007385269975	24.98366879636184	27.08362259100521	24.392634759933205
94-95	23.09872944257485	24.97345901245551	27.253975843723026	24.673835701246613
96-97	23.26652132763192	24.86517493348731	27.019572272828192	24.848731466052577
98-99	23.047265425199388	24.866880103055678	27.05423683861557	25.031617633129372
100-101	23.27228033005146	25.160475360183927	26.74329170938558	24.823952600379034
102-103	23.47893561107794	24.913311529869635	26.890020823419224	24.717732035633205
104-105	23.37026371627766	25.026826311003337	26.784934828735977	24.817975143983027
106-107	23.342624126051657	25.013451188478815	26.940873880141197	24.70305080532834
108-109	23.338623063526782	24.977454230192688	26.766110562939456	24.917812143341077
110-111	23.16290503843219	25.284461847063916	26.636541307687118	24.91609180681678
112-113	23.14100441271085	25.41124297796979	26.535677180230028	24.912075429089338
114-115	23.166314289828055	25.327483120012733	26.630595403187307	24.87560718697191
116-117	23.129144994483443	25.367290461814523	26.58062961481104	24.922934928890992
118-119	22.937282850441974	25.21265027601911	26.738276291909628	25.11179058162929
120-121	22.88091925593858	25.16307032938388	26.87758207317554	25.078428341502008
122-123	22.89001239698229	25.42358882028619	26.777709074045447	24.908689708686072
124-125	22.89091531835183	25.385853454494757	26.687010105541376	25.036221121612034
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	267.0
1	225.5
2	337.0
3	519.5
4	408.0
5	337.5
6	548.0
7	1044.0
8	1464.5
9	1560.0
10	1398.5
11	1099.0
12	1012.0
13	991.0
14	970.5
15	971.5
16	945.5
17	901.0
18	870.5
19	897.5
20	915.5
21	906.0
22	926.0
23	1016.0
24	1149.5
25	1278.0
26	1512.5
27	1944.5
28	2549.5
29	3322.0
30	4277.0
31	5281.0
32	6431.0
33	7457.5
34	8317.0
35	9299.5
36	10332.0
37	11512.5
38	12561.5
39	13282.0
40	13983.5
41	14615.5
42	15297.0
43	16870.5
44	18708.0
45	19918.5
46	21735.5
47	23854.5
48	25999.0
49	27792.0
50	28392.0
51	27802.0
52	27274.0
53	27245.5
54	27119.5
55	26634.0
56	25277.5
57	23369.0
58	21587.5
59	19539.5
60	16910.0
61	13993.0
62	11182.0
63	8767.0
64	6701.0
65	5445.5
66	4278.0
67	3173.0
68	2603.0
69	2221.0
70	1847.0
71	1600.0
72	1464.5
73	1444.0
74	1174.0
75	913.5
76	707.5
77	477.5
78	358.0
79	231.0
80	160.0
81	96.0
82	66.5
83	55.5
84	26.5
85	11.5
86	8.0
87	4.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04288931525616894
2	0.0
3	0.0
4	6.062094029140486E-4
5	0.0034857040667557798
6	0.0010608664550995851
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.819855782783047E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	2.273285260927682E-4
34-35	3.031047014570243E-4
36-37	0.0024248376116561944
38-39	0.0017428520333778899
40-41	0.004546570521855364
42-43	0.0016670758580136337
44-45	2.273285260927682E-4
46-47	4.546570521855364E-4
48-49	0.0
50-51	0.0
52-53	7.577617536425608E-5
54-55	4.546570521855364E-4
56-57	0.0
58-59	0.0013639711565566093
60-61	7.577617536425608E-5
62-63	0.0
64-65	7.577617536425608E-5
66-67	3.031047014570243E-4
68-69	0.0
70-71	0.0016670758580136337
72-73	1.5155235072851215E-4
74-75	0.07388177098014968
76-77	0.07653393711789865
78-79	0.0016670758580136337
80-81	0.0
82-83	0.0010608664550995851
84-85	0.08479354023260255
86-87	0.158902639738845
88-89	0.19171372367156786
90-91	0.06812278165246621
92-93	0.12480336082492975
94-95	0.0014397473319208655
96-97	2.273285260927682E-4
98-99	7.577617536425608E-5
100-101	2.273285260927682E-4
102-103	6.062094029140486E-4
104-105	0.005758989327683462
106-107	0.006819855782783046
108-109	0.010457112200267338
110-111	0.00598631785377623
112-113	0.00598631785377623
114-115	0.005001227574040901
116-117	9.093141043710728E-4
118-119	0.0015912996826493774
120-121	0.0
122-123	0.0
124-125	3.031047014570243E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	659838.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.70546192790766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.27511676042766	68.69028379441536
2	9.028299472849245	14.211529608681703
3	1.915169323066078	4.522028587262217
4	0.6976291291413828	2.196288914537459
5	0.33538989140274095	1.3198508164401757
6	0.21046389194886664	0.9938794700988476
7	0.130036496573348	0.7164207771204497
8	0.09111283818478336	0.5736862413516863
9	0.05125560388467565	0.36306863821235297
>10	0.242404760401484	3.1681666266421176
>50	0.015187884318535946	0.8353307868972124
>100	0.0068005266867285615	1.1423775729472734
>500	4.5336844577519047E-4	0.20000082137507533
>1k	6.800526686627856E-4	1.0670873440181317
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2315	0.3508436919365056	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2061	0.31234939485146357	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1605	0.243241522919262	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.5155235072851215E-4	0.0
6	0.0	0.0	0.0	1.5155235072851215E-4	0.0
7	0.0	0.0	0.0	1.5155235072851215E-4	0.0
8	0.0	0.0	0.0	1.5155235072851215E-4	0.0
9	0.0	0.0	0.0	1.5155235072851215E-4	4.5465705218553646E-4
10-11	0.0	0.0	0.0	1.5155235072851215E-4	4.5465705218553646E-4
12-13	0.0	0.0	0.0	3.031047014570243E-4	4.5465705218553646E-4
14-15	0.0	0.0	0.0	4.5465705218553646E-4	5.304332275497926E-4
16-17	0.0	0.0	0.0	5.304332275497926E-4	6.062094029140486E-4
18-19	0.0	0.0	0.0	6.062094029140486E-4	6.062094029140486E-4
20-21	0.0	0.0	0.0	9.093141043710729E-4	6.062094029140486E-4
22-23	0.0	0.0	0.0	0.002273285260927682	6.062094029140486E-4
24-25	0.0	0.0	0.0	0.005228556100133669	6.062094029140486E-4
26-27	0.0	0.0	0.0	0.009168917219074986	6.062094029140486E-4
28-29	0.0	0.0	0.0	0.019171372367156787	6.062094029140486E-4
30-31	0.0	0.0	0.0	0.038266968558949316	6.062094029140486E-4
32-33	0.0	0.0	0.0	0.0693352004582943	6.062094029140486E-4
34-35	0.0	0.0	0.0	0.10525310758095169	6.062094029140486E-4
36-37	0.0	0.0	0.0	0.1587510873881165	6.062094029140486E-4
38-39	0.0	0.0	0.0	0.22982913987978867	6.062094029140486E-4
40-41	0.0	0.0	0.0	0.31098542369490695	6.062094029140486E-4
42-43	0.0	0.0	0.0	0.38395788057068553	6.062094029140486E-4
44-45	0.0	0.0	0.0	0.4585974133044778	6.062094029140486E-4
46-47	0.0	0.0	0.0	0.5522567660546983	6.062094029140486E-4
48-49	0.0	0.0	0.0	0.6447036999990907	9.093141043710729E-4
50-51	0.0	0.0	0.0	0.7466984320393794	9.093141043710729E-4
52-53	0.0	0.0	0.0	0.8422521891737063	9.093141043710729E-4
54-55	0.0	1.5155235072851215E-4	0.0	0.93863948423704	9.093141043710729E-4
56-57	0.0	1.5155235072851215E-4	0.0	1.0388155880685865	9.093141043710729E-4
58-59	0.0	1.5155235072851215E-4	0.0	1.148691042346758	9.093141043710729E-4
60-61	0.0	1.5155235072851215E-4	0.0	1.260612453359764	9.093141043710729E-4
62-63	0.0	1.5155235072851215E-4	0.0	1.3740493878800555	9.093141043710729E-4
64-65	0.0	1.5155235072851215E-4	0.0	1.4838490659828625	9.093141043710729E-4
66-67	0.0	1.5155235072851215E-4	0.0	1.6037269754091157	9.093141043710729E-4
68-69	0.0	1.5155235072851215E-4	0.0	1.7458073042170956	9.093141043710729E-4
70-71	0.0	1.5155235072851215E-4	0.0	1.8996329402065355	9.093141043710729E-4
72-73	0.0	1.5155235072851215E-4	0.0	2.0383033411231244	9.093141043710729E-4
74-75	0.0	1.5155235072851215E-4	0.0	2.1803836699311043	9.093141043710729E-4
76-77	0.0	1.5155235072851215E-4	0.0	2.3332242156408087	9.093141043710729E-4
78-79	0.0	1.5155235072851215E-4	0.0	2.5033417293335636	9.093141043710729E-4
80-81	0.0	1.5155235072851215E-4	0.0	2.684295236103407	9.093141043710729E-4
82-83	0.0	1.5155235072851215E-4	0.0	2.8790400067895456	9.093141043710729E-4
84-85	0.0	1.5155235072851215E-4	0.0	3.0605997229623028	9.093141043710729E-4
86-87	0.0	1.5155235072851215E-4	0.0	3.2441296196945313	9.093141043710729E-4
88-89	0.0	1.5155235072851215E-4	0.0	3.446527784092459	9.093141043710729E-4
90-91	0.0	1.5155235072851215E-4	0.0	3.659534613041383	9.093141043710729E-4
92-93	0.0	1.5155235072851215E-4	0.0	3.886635810608058	9.093141043710729E-4
94-95	0.0	1.5155235072851215E-4	0.0	4.108887332951422	9.093141043710729E-4
96-97	0.0	1.5155235072851215E-4	0.0	4.3326543788020695	9.093141043710729E-4
98-99	0.0	1.5155235072851215E-4	0.0	4.580821353120008	9.093141043710729E-4
100-101	0.0	1.5155235072851215E-4	0.0	4.846871504823911	9.093141043710729E-4
102-103	0.0	1.5155235072851215E-4	0.0	5.114512956210463	9.093141043710729E-4
104-105	0.0	1.5155235072851215E-4	0.0	5.402083541717816	9.093141043710729E-4
106-107	0.0	1.5155235072851215E-4	0.0	5.683743585546756	9.093141043710729E-4
108-109	0.0	1.5155235072851215E-4	0.0	5.972299261333843	0.0010608664550995851
110-111	0.0	1.5155235072851215E-4	0.0	6.282072266222921	0.001136642630463841
112-113	0.0	1.5155235072851215E-4	0.0	6.615260109299555	0.0012124188058280972
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2315	0.0	42.41791	1
GTATCAA	3315	0.0	37.521404	1
ATCAACG	4220	0.0	29.320383	3
TATCAAC	4330	0.0	28.987673	2
TCAACGC	4290	0.0	28.703299	4
CAACGCA	4310	0.0	28.570103	5
AACGCAG	4565	0.0	27.100388	6
ACGCAGA	5060	0.0	24.566805	7
CGCAGAG	5195	0.0	24.042892	8
CATGGGG	2370	0.0	23.844818	4
GCAGAGT	5600	0.0	22.304073	9
ATGGGGG	1265	0.0	21.63147	5
GTACATG	4680	0.0	20.982363	1
ACATGGG	4495	0.0	20.77728	3
ATTTAGG	230	6.1205355E-6	20.700386	1
TACATGG	4760	0.0	20.495365	2
CAGAGTA	5630	0.0	20.17799	10-11
AGAGTAC	5430	0.0	20.044914	10-11
ATTACTC	150	0.0047099693	19.82885	3
GAGTACT	2480	0.0	19.306215	12-13
>>END_MODULE