FastQCFastQC Report
Sun 1 Jan 2017
ERR1142712_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142712_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580040
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14590.2515343769395214No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11160.19240052410178607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20600.028.626711
GTATCAA27950.028.1316721
ATCAACG32900.023.8990963
AACGCAG33450.023.328066
CAACGCA33850.023.0523955
TCAACGC33650.023.0123924
TATCAAC35050.022.9429452
CTATAGT1350.002537202622.0617354
CATGGGT9300.021.777074
ACGCAGA36150.021.4209387
GTACAAA4800.021.0965351
CATGGGG23700.020.8609074
CGCAGAG37300.020.7605088
GTACATG47450.020.4623181
ATAGGCA1754.743485E-420.4228654
TACATGG48200.020.143922
ACATGGG47950.020.0004923
GCAGAGT39100.019.9571259
ATGGGGG13900.019.7127175
TGGGGGG9150.019.5300626
Produced by FastQC (version 0.11.5)