##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142712_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	580040
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.247684642438454	33.0	33.0	33.0	27.0	33.0
2	31.383525274119027	33.0	33.0	33.0	27.0	33.0
3	31.42987897386387	33.0	33.0	33.0	27.0	33.0
4	31.39307116750569	33.0	33.0	33.0	27.0	33.0
5	31.424756913316322	33.0	33.0	33.0	27.0	33.0
6	34.74362112957727	37.0	37.0	37.0	27.0	37.0
7	34.721283014964484	37.0	37.0	37.0	27.0	37.0
8	34.70430659954486	37.0	37.0	37.0	27.0	37.0
9	34.63934039031791	37.0	37.0	37.0	27.0	37.0
10-11	34.71230949589683	37.0	37.0	37.0	27.0	37.0
12-13	34.679146265774776	37.0	37.0	37.0	27.0	37.0
14-15	34.67499396593338	37.0	37.0	37.0	27.0	37.0
16-17	34.698068236673336	37.0	37.0	37.0	27.0	37.0
18-19	34.66912885318254	37.0	37.0	37.0	27.0	37.0
20-21	34.69950520653748	37.0	37.0	37.0	27.0	37.0
22-23	34.777227432590855	37.0	37.0	37.0	27.0	37.0
24-25	34.81536187159506	37.0	37.0	37.0	27.0	37.0
26-27	34.87264757602924	37.0	37.0	37.0	27.0	37.0
28-29	34.89843631473691	37.0	37.0	37.0	27.0	37.0
30-31	34.92335873388042	37.0	37.0	37.0	27.0	37.0
32-33	34.91557823598372	37.0	37.0	37.0	27.0	37.0
34-35	34.92458192538446	37.0	37.0	37.0	27.0	37.0
36-37	34.90823908695952	37.0	37.0	37.0	27.0	37.0
38-39	34.87802910144128	37.0	37.0	37.0	27.0	37.0
40-41	34.85352561892283	37.0	37.0	37.0	27.0	37.0
42-43	34.837397420867525	37.0	37.0	37.0	27.0	37.0
44-45	34.8434564857596	37.0	37.0	37.0	27.0	37.0
46-47	34.83842924625888	37.0	37.0	37.0	27.0	37.0
48-49	34.80069391766085	37.0	37.0	37.0	27.0	37.0
50-51	34.78479156609889	37.0	37.0	37.0	27.0	37.0
52-53	34.745649437969796	37.0	37.0	37.0	27.0	37.0
54-55	34.7313512861182	37.0	37.0	37.0	27.0	37.0
56-57	34.68962485345838	37.0	37.0	37.0	27.0	37.0
58-59	34.65948986276808	37.0	37.0	37.0	27.0	37.0
60-61	34.63789824839666	37.0	37.0	37.0	27.0	37.0
62-63	34.575199124198335	37.0	37.0	37.0	27.0	37.0
64-65	34.553298048410454	37.0	37.0	37.0	27.0	37.0
66-67	34.505372905316875	37.0	37.0	37.0	27.0	37.0
68-69	34.47064771395076	37.0	37.0	37.0	27.0	37.0
70-71	34.434256258189095	37.0	37.0	37.0	27.0	37.0
72-73	34.38013671470933	37.0	37.0	37.0	27.0	37.0
74-75	34.32754465209295	37.0	37.0	37.0	27.0	37.0
76-77	34.27162437073305	37.0	37.0	37.0	27.0	37.0
78-79	34.23005827184332	37.0	37.0	37.0	27.0	37.0
80-81	34.1707485690642	37.0	37.0	37.0	27.0	37.0
82-83	34.1398334597614	37.0	37.0	37.0	27.0	37.0
84-85	34.08198055306531	37.0	37.0	37.0	27.0	37.0
86-87	34.00256275429281	37.0	37.0	37.0	27.0	37.0
88-89	33.95359802772222	37.0	37.0	37.0	27.0	37.0
90-91	33.87367078132543	37.0	37.0	37.0	24.5	37.0
92-93	33.81592303979036	37.0	37.0	37.0	22.0	37.0
94-95	33.76386111302669	37.0	33.0	37.0	22.0	37.0
96-97	33.64748896627819	37.0	33.0	37.0	22.0	37.0
98-99	33.56502223984553	37.0	33.0	37.0	22.0	37.0
100-101	33.50515481690918	37.0	33.0	37.0	22.0	37.0
102-103	33.43541910902697	37.0	33.0	37.0	22.0	37.0
104-105	33.25798220812358	37.0	33.0	37.0	14.0	37.0
106-107	33.16744707261568	37.0	33.0	37.0	14.0	37.0
108-109	33.07044083166679	37.0	33.0	37.0	14.0	37.0
110-111	32.965379111785396	37.0	33.0	37.0	14.0	37.0
112-113	32.86936159575202	37.0	33.0	37.0	14.0	37.0
114-115	32.74916385076891	37.0	33.0	37.0	14.0	37.0
116-117	32.652809289014556	37.0	33.0	37.0	14.0	37.0
118-119	32.52112440521343	37.0	33.0	37.0	14.0	37.0
120-121	32.39621491621267	37.0	33.0	37.0	14.0	37.0
122-123	32.283076167160885	37.0	33.0	37.0	14.0	37.0
124-125	30.670475312047444	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1041.0
1	73.0
2	31.0
3	22.0
4	22.0
5	19.0
6	16.0
7	18.0
8	14.0
9	21.0
10	11.0
11	23.0
12	18.0
13	17.0
14	45.0
15	209.0
16	366.0
17	622.0
18	1204.0
19	2451.0
20	3609.0
21	4529.0
22	4897.0
23	5186.0
24	5822.0
25	6537.0
26	7788.0
27	9389.0
28	11013.0
29	13142.0
30	15886.0
31	19241.0
32	24620.0
33	32513.0
34	49399.0
35	91139.0
36	269087.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.125190010669996	19.318399950195158	12.170367155088481	24.386042884046365
2	17.57380767561792	22.537078880899816	35.95359153846021	23.93552190502205
3	19.98513332468992	26.94619473615973	27.933791434374868	25.134880504775488
4	14.890482933179808	18.929362399572618	33.69069662501751	32.489458042230076
5	15.714377108394153	37.164277945786495	30.87181828084718	16.24952666497217
6	30.087498962052646	35.31875363281574	18.303855628442527	16.289891776689085
7	26.6614764450442	32.892164495423955	21.31883531426792	19.12752374526392
8	25.684597338426723	33.67225832347181	20.363007868457657	20.280136469643807
9	26.661914837298863	18.25065784579506	19.60527522261225	35.48215209429383
10-11	25.284750257998827	26.927638357008092	25.53734257313715	22.25026881185592
12-13	26.094828943520998	25.76124588428059	25.103855410430008	23.040069761768407
14-15	24.71045376732325	26.138008171047023	23.279093243877984	25.872444817751745
16-17	24.30334267569666	27.869232272130766	24.02895037597105	23.798474676201526
18-19	24.29636391297733	27.158019031022224	25.392964330664007	23.15265272533643
20-21	25.957820464731984	26.01598542505363	24.772568955295085	23.253625154919302
22-23	25.694332216479033	25.833816573749115	24.681903935550274	23.78994727422158
24-25	24.856985337324495	26.422023739569866	24.6972928318113	24.02369809129434
26-27	24.458356306919036	26.452866844233043	24.79358341269663	24.29519343615129
28-29	24.394094271491735	26.369871003363816	25.07852213352089	24.157512591623558
30-31	24.837272239460606	26.22818091404051	25.06737659433458	23.867170252164303
32-33	24.316439583723998	26.348390486453226	24.92220533298537	24.4129645968374
34-35	24.530413188005813	26.497242705829287	25.015769545163113	23.956574561001784
36-37	24.541512109264634	26.558642815359747	24.823884486541083	24.07596058883454
38-39	24.450677402100947	26.416478511417633	24.825259723370742	24.307584363110685
40-41	24.239929224213753	26.48873652434905	25.102422722897938	24.168911528539265
42-43	24.414061487470775	26.474069747549194	24.981812275029505	24.130056489950526
44-45	24.395762562505833	26.49167337657625	24.87334685682493	24.239217204092984
46-47	24.21620201939916	26.418240425478608	24.944599139677557	24.42095841544468
48-49	24.32388532445383	26.69588101833212	24.918741824503076	24.061491832710974
50-51	24.074165277730977	26.74705644997542	24.945457813076473	24.233320459217133
52-53	24.08334485380521	26.56615054952651	24.868407409967514	24.48209718670077
54-55	23.943784878491737	26.304657550342963	25.175845054176953	24.575712516988347
56-57	24.1131792148233	26.148307314278902	24.824242864091804	24.914270606805992
58-59	24.058686762300642	26.30605348717253	25.035532258106326	24.599727492420506
60-61	24.205815008424416	26.26292824124077	25.18892297057934	24.342333779755478
62-63	23.761594324991037	26.374189423297086	25.195553654669485	24.668662597042392
64-65	23.8250110167368	25.980835198258063	25.32268238099764	24.8714714040075
66-67	24.123191743660787	25.991964553465795	25.11401121574802	24.770832487125396
68-69	24.023993141853705	25.977130294409434	25.028517997448933	24.970358566287924
70-71	23.837691629651395	25.96233948046112	25.261757030021258	24.93821185986623
72-73	24.105415986103548	26.091363978118277	25.270799305177466	24.53242073060071
74-75	24.022515355503565	25.93103138339108	25.43097823221791	24.615475028887445
76-77	23.992008137043857	25.56246797162405	25.47786552964672	24.967658361685377
78-79	23.917633038733523	25.801464443643635	25.491655021807645	24.789247495815196
80-81	23.77525810078266	25.87393914464446	25.590756332294333	24.760046422278545
82-83	23.87112048927761	25.822994596033173	25.64827728035476	24.657607634334457
84-85	23.794745341110463	26.03215279619772	25.27321755193272	24.8998843107591
86-87	23.65844619314643	26.074223558753477	25.42172869240996	24.84560155569013
88-89	23.76921588478589	25.67560096610679	25.44599511418097	25.10918803492635
90-91	24.14599211086941	25.62896205926005	25.34499828083992	24.880047549030618
92-93	23.83854875283447	25.996155922686537	25.356743332253533	24.80855199222546
94-95	23.362062561652095	26.101704577846192	25.49334632454181	25.04288653595991
96-97	23.51404852467172	25.998752854443257	25.382694041684374	25.104504579200654
98-99	23.39663221721724	26.17395172470384	25.304409040065302	25.125007018013623
100-101	23.61805355811746	26.483394703269237	25.148544612617936	24.75000712599537
102-103	23.715293822959087	26.342188114065586	25.457717738208864	24.484800324766468
104-105	23.604986347235453	26.45984376849127	25.297566343970196	24.637603540303083
106-107	23.647923796230234	26.370733274713277	25.381044761422704	24.60029816763378
108-109	23.600103313727335	26.37894887784534	25.347712024214804	24.673235784212523
110-111	23.376237504037924	26.939281759652573	25.111895758585007	24.572584977724496
112-113	23.36989990473287	26.988150772544756	25.075725710120167	24.566223612602208
114-115	23.316021451416894	26.969827575038067	25.123533938687782	24.590617034857257
116-117	23.312460052859784	26.892242049439446	25.162466098913438	24.632831798787336
118-119	23.141042638146228	26.708889950276944	25.350639524690084	24.799427886886743
120-121	23.241630100542444	26.702829699754687	25.48828732335971	24.567252876343158
122-123	23.134532037318863	26.85095775976286	25.533984104418185	24.480526098500093
124-125	23.191331835306944	26.812080623883933	25.213494677859533	24.78309286294959
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1896.0
1	1180.0
2	364.0
3	231.0
4	170.5
5	144.5
6	144.0
7	131.0
8	124.5
9	132.0
10	115.5
11	103.0
12	108.5
13	117.5
14	126.5
15	137.0
16	166.0
17	206.0
18	243.5
19	270.0
20	316.5
21	413.5
22	523.0
23	675.0
24	859.0
25	1086.5
26	1433.5
27	1841.5
28	2279.5
29	2955.5
30	3842.0
31	4601.0
32	5652.5
33	6690.0
34	7442.0
35	8446.5
36	9379.0
37	10556.5
38	11649.0
39	12292.5
40	12798.0
41	13247.0
42	14048.0
43	15306.5
44	16989.5
45	18522.0
46	20122.5
47	21824.5
48	23583.0
49	25084.0
50	25727.5
51	25261.0
52	24532.5
53	24486.0
54	24316.5
55	23496.0
56	22285.0
57	20189.5
58	18145.0
59	16491.0
60	14296.0
61	12092.0
62	9970.0
63	7813.5
64	6020.5
65	4728.0
66	3749.0
67	2967.0
68	2328.0
69	1931.0
70	1708.0
71	1538.0
72	1311.0
73	1219.0
74	1017.0
75	800.0
76	637.0
77	456.0
78	322.0
79	208.0
80	128.0
81	92.5
82	60.0
83	35.5
84	27.0
85	17.0
86	8.5
87	2.0
88	1.0
89	0.0
90	1.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3073925936142335
2	0.2777394662437073
3	0.26980897869112475
4	0.28222191572994965
5	0.2925660299289704
6	0.34066616095441693
7	0.3499758637335356
8	0.3510102751534377
9	0.34773463899041446
10-11	0.35057927039514514
12-13	0.35738914557616713
14-15	0.34885525136197504
16-17	0.2880835804427281
18-19	0.27860147576029237
20-21	0.2608440797186401
22-23	0.2552410178608372
24-25	0.246189917936694
26-27	0.2546376111992277
28-29	0.23817322943245292
30-31	0.23955244465898906
32-33	0.2471381284049376
34-35	0.24015585132059858
36-37	0.2364492103992828
38-39	0.24058685607889113
40-41	0.22601889524860355
42-43	0.2332597751879181
44-45	0.22308806289221433
46-47	0.22472588097372598
48-49	0.22912212950830976
50-51	0.23438038755947865
52-53	0.23446658851113714
54-55	0.23007033997655335
56-57	0.22920833045996825
58-59	0.23110475139645542
60-61	0.23532859802772224
62-63	0.2348975932694297
64-65	0.23705261706089237
66-67	0.25489621405420315
68-69	0.2513619750362044
70-71	0.24998275980966828
72-73	0.2542928073925936
74-75	0.25886145783049447
76-77	0.2502413626646438
78-79	0.2514481759878629
80-81	0.24800013792152265
82-83	0.23981104751396456
84-85	0.23007033997655335
86-87	0.21748500103441143
88-89	0.21067512585338943
90-91	0.21877801530928903
92-93	0.21162333632163297
94-95	0.204641059237294
96-97	0.1927453279084201
98-99	0.2021412316391973
100-101	0.2023136335425143
102-103	0.20041721260602718
104-105	0.2102441210950969
106-107	0.2018826287842218
108-109	0.21041652299841393
110-111	0.19877939452451554
112-113	0.19679677263636988
114-115	0.198348389766223
116-117	0.1982621888145645
118-119	0.19541755740983383
120-121	0.2034342459140749
122-123	0.19602096407144334
124-125	0.22041583339080062
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	580040.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.33002782532361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.18211567365937	70.83671805760962
2	8.456875572888807	13.586821001709149
3	1.7988256220576153	4.3349913681837995
4	0.6074518039092011	1.951864812422766
5	0.2985071185625646	1.1989542570093996
6	0.15878597723365503	0.7653169181670426
7	0.10185841773906193	0.5727602671855596
8	0.07596305150046423	0.48816912325830253
9	0.04885713601676016	0.3532225586122768
>10	0.24577589617628529	3.5478050319962566
>50	0.017738450492733128	0.9862121870991404
>100	0.006745605297034342	0.8603765454087852
>500	0.0	0.0
>1k	4.996744664463447E-4	0.516787871337947
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1459	0.2515343769395214	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1116	0.19240052410178607	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	1.724019033170126E-4	1.724019033170126E-4
7	0.0	0.0	0.0	1.724019033170126E-4	1.724019033170126E-4
8	0.0	0.0	0.0	1.724019033170126E-4	1.724019033170126E-4
9	0.0	0.0	0.0	1.724019033170126E-4	1.724019033170126E-4
10-11	0.0	0.0	0.0	2.586028549755189E-4	1.724019033170126E-4
12-13	0.0	0.0	0.0	3.448038066340252E-4	1.724019033170126E-4
14-15	0.0	0.0	0.0	3.448038066340252E-4	1.724019033170126E-4
16-17	0.0	0.0	0.0	6.896076132680505E-4	1.724019033170126E-4
18-19	0.0	0.0	0.0	0.0010344114199020756	1.724019033170126E-4
20-21	0.0	0.0	0.0	0.0018964209364871387	1.724019033170126E-4
22-23	0.0	0.0	0.0	0.0034480380663402524	1.724019033170126E-4
24-25	0.0	0.0	0.0	0.005689262809461417	1.724019033170126E-4
26-27	0.0	0.0	0.0	0.009223501827460175	1.724019033170126E-4
28-29	0.0	0.0	0.0	0.017240190331701263	1.724019033170126E-4
30-31	0.0	0.0	0.0	0.039221433004620374	1.724019033170126E-4
32-33	0.0	0.0	0.0	0.0683573546651955	1.724019033170126E-4
34-35	0.0	0.0	0.0	0.10602717053996277	1.724019033170126E-4
36-37	0.0	0.0	0.0	0.15929935866491968	1.724019033170126E-4
38-39	0.0	0.0	0.0	0.23446658851113716	1.724019033170126E-4
40-41	0.0	0.0	0.0	0.32454658299427624	1.724019033170126E-4
42-43	0.0	0.0	0.0	0.3984207985656162	2.586028549755189E-4
44-45	0.0	0.0	0.0	0.47531204744500377	3.448038066340252E-4
46-47	0.0	0.0	0.0	0.5723743190124819	3.448038066340252E-4
48-49	0.0	0.0	0.0	0.6664195572719123	6.896076132680505E-4
50-51	0.0	0.0	0.0	0.7639990345493415	6.896076132680505E-4
52-53	0.0	0.0	0.0	0.861492310875112	8.620095165850631E-4
54-55	0.0	0.0	0.0	0.9567443624577616	8.620095165850631E-4
56-57	0.0	0.0	0.0	1.0554444521067512	8.620095165850631E-4
58-59	0.0	0.0	0.0	1.1615578235983726	8.620095165850631E-4
60-61	0.0	0.0	0.0	1.2755154816909178	8.620095165850631E-4
62-63	0.0	0.0	0.0	1.3801634370043445	8.620095165850631E-4
64-65	0.0	0.0	0.0	1.4861044065926488	8.620095165850631E-4
66-67	0.0	0.0	0.0	1.606527136059582	8.620095165850631E-4
68-69	0.0	0.0	0.0	1.7372077787738776	8.620095165850631E-4
70-71	0.0	0.0	0.0	1.8772843252189504	8.620095165850631E-4
72-73	0.0	0.0	0.0	2.0172746707123648	0.0010344114199020756
74-75	0.0	0.0	0.0	2.148127715329977	0.0010344114199020756
76-77	0.0	0.0	0.0	2.2969967588442177	0.0010344114199020756
78-79	0.0	0.0	0.0	2.462933590786842	0.0010344114199020756
80-81	0.0	0.0	0.0	2.639473139783463	0.0010344114199020756
82-83	0.0	0.0	0.0	2.8230811668160816	0.0010344114199020756
84-85	0.0	0.0	0.0	2.9982415005861665	0.0010344114199020756
86-87	0.0	0.0	0.0	3.1752120543410802	0.0010344114199020756
88-89	0.0	0.0	0.0	3.36562995655472	0.0010344114199020756
90-91	0.0	0.0	0.0	3.567340183435625	0.0010344114199020756
92-93	0.0	0.0	0.0	3.7810323425970624	0.0010344114199020756
94-95	0.0	0.0	0.0	3.9851561961244055	0.0010344114199020756
96-97	0.0	0.0	0.0	4.192297082959795	0.0010344114199020756
98-99	0.0	0.0	0.0	4.42693607337425	0.0010344114199020756
100-101	0.0	0.0	0.0	4.679073856975381	0.0010344114199020756
102-103	0.0	0.0	0.0	4.938538721467485	0.0012068133232190884
104-105	0.0	0.0	0.0	5.207313288738708	0.0012068133232190884
106-107	0.0	0.0	0.0	5.476518860768223	0.0012068133232190884
108-109	0.0	0.0	0.0	5.763309426936074	0.0013792152265361011
110-111	0.0	0.0	0.0	6.068978001517136	0.0015516171298531136
112-113	0.0	0.0	0.0	6.382835666505759	0.0015516171298531136
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2060	0.0	28.62671	1
GTATCAA	2795	0.0	28.131672	1
ATCAACG	3290	0.0	23.899096	3
AACGCAG	3345	0.0	23.32806	6
CAACGCA	3385	0.0	23.052395	5
TCAACGC	3365	0.0	23.012392	4
TATCAAC	3505	0.0	22.942945	2
CTATAGT	135	0.0025372026	22.061735	4
CATGGGT	930	0.0	21.77707	4
ACGCAGA	3615	0.0	21.420938	7
GTACAAA	480	0.0	21.096535	1
CATGGGG	2370	0.0	20.860907	4
CGCAGAG	3730	0.0	20.760508	8
GTACATG	4745	0.0	20.462318	1
ATAGGCA	175	4.743485E-4	20.422865	4
TACATGG	4820	0.0	20.14392	2
ACATGGG	4795	0.0	20.000492	3
GCAGAGT	3910	0.0	19.957125	9
ATGGGGG	1390	0.0	19.712717	5
TGGGGGG	915	0.0	19.530062	6
>>END_MODULE