FastQCFastQC Report
Sun 1 Jan 2017
ERR1142712_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142712_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580040
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24690.42566029928970417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.3851458520102062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16510.28463554237638783No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.13274946555409972No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25050.035.1486241
GTATCAA34200.032.181031
TATCAAC43600.025.5114152
ATCAACG43350.025.3841193
CAACGCA45150.024.3721285
TCAACGC45150.024.3721284
AACGCAG47050.023.514346
CATGGGT8550.022.9576424
CATGGGG21450.022.738724
ACGCAGA52800.020.8409397
TAGCACC2004.9157148E-520.8184094
CGCAGAG54000.020.7082568
ATGGGTA3852.3101165E-1020.0845475
ACATGGG45400.019.9143983
GTACATG47950.019.7270831
GCAGAGT57000.019.618359
TACATGG48800.019.3801332
ATGGGGG14100.018.9833495
TACTACA2851.8576466E-618.7835252
GAACCGT1600.006841168318.5878646
Produced by FastQC (version 0.11.5)