FastQCFastQC Report
Sun 1 Jan 2017
ERR1142711_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142711_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180495
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3420.18947893293443033No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2850.15789911077869193No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA2660.14737250339344582No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC2300.1274273525582426No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC2220.12299509681708634No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA2160.11967090501121916No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC2140.1185628410759301No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2100.11634671320535195No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2030.11246848943184022No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT2010.11136042549655115No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT1990.11025236156126207No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1930.10692816975539489No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA1890.10471204188481677No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAT600.001299015439.707331
GTGTTTA758.11201E-539.7073251
CATGGGT1304.1316525E-836.6529164
ATGGGGC851.6945323E-435.0358735
ATGGGTA902.3707164E-433.089445
TATTCTC1403.0508854E-629.7804955
GTACAAA1254.8262395E-528.5892751
ACACAGT900.00944784726.478916
GTAGGAC900.00946066126.4715523
ATGCCGA450.009524452526.44216384-85
GAACAAA1401.0437891E-425.5261381
GTATTTC1651.0943377E-525.26831
GGTATCA12850.025.0295181
TCTCTGC1751.7226712E-523.8310188
TTCTCTG1852.647454E-522.5428547
TCTTACC1602.576607E-422.341589
CATAATG2403.4938603E-722.3353734
TACAAAA2153.365787E-622.1622282
ACCAGGC1350.002527822.0657587
ATCTTAC1653.172671E-421.664568
Produced by FastQC (version 0.11.5)