FastQCFastQC Report
Sun 1 Jan 2017
ERR1142711_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142711_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180495
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.7058367267791352No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10660.5905980775090722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.3900385052217513No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.20332973212554367No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC2860.15845314274633646No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA2770.15346685503753568No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA2350.13019751239646526No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC2280.12631928862295355No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.12022493697886369No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG2080.11523864927006289No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA2060.11413058533477381No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT2020.11191445746419569No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC1990.11025236156126207No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.10859026565832848No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1940.10748220172303942No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1940.10748220172303942No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1910.10582010582010583No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1900.1052660738524613No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1870.10360397794952768No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC201.7728955E-459.48267726-27
GGTATCA12150.047.4882351
GTATCAA17100.034.089492
CGATTGA350.002790444333.990156-57
GGATCCC452.3938439E-433.04593338-39
CTTAATA800.00535799629.7413392
TATCAAC20000.028.8491023
TACTGAT1254.8697926E-528.551697
TACCAGT1055.8962416E-428.3250891
ATCAACG21100.027.6270284
CAACGCA21400.026.683826
TCCGAAG450.009533985526.43674766-67
GGGGTGC450.009533985526.43674720-21
TCAACGC23350.024.9648955
ATGGGAC1200.001285840124.784455
CCAGGGT600.001288335224.7844598-99
AACGCAG23300.024.5078876
CTGATTG1250.001630974723.7930749
GAGTACT10950.023.35849812-13
CGCAGAG24650.022.9243588
Produced by FastQC (version 0.11.5)