FastQCFastQC Report
Sun 1 Jan 2017
ERR1142700_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142700_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133431
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4350.3260111967983452No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3510.26305731052004405No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2590.19410781602476188No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2370.17761989342806395No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2330.1746220893195734No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2150.16113197083136602No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1910.14314514618042284No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1770.132652831800706No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1700.12740667461084754No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1700.12740667461084754No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC1680.12590777255660227No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1530.1146660071497628No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA1520.11391655612264016No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT1510.11316710509551753No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1460.10941984995990438No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT1360.10192533968867805No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1350.10117588866155541No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG350.00776749551.0227242
CTCTAAT350.00776749551.0227241
GTACACT600.001301491339.6843454
TATTCTC905.105483E-639.6843453
CATACAC600.001301491339.6843453
ATGGGAC650.001928523936.6317027
TACACTG902.3754616E-433.0702865
GTACAAA1102.0201478E-532.469011
TCCGCTT1004.4062705E-429.7632569
TAAGTAC850.007172198528.0124781
ATACTGA850.007172198528.0124786
TCTAATT1107.694975E-427.0575082
CGATTCA450.00953028126.43634456-57
GAGTACT1256.91216E-1026.19166612-13
TTCTCTG1159.975372E-425.8810925
ATGGGGG5150.025.4288025
CATGGGG6850.025.2010044
TGGGGGG3103.274181E-1023.0425226
TCTCTGC1400.00313307721.2594686
GATACTG700.003148096821.25147690-91
Produced by FastQC (version 0.11.5)