FastQCFastQC Report
Sun 1 Jan 2017
ERR1142700_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142700_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133431
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.697738906251171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.6939916511155578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.36423319918159947No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3940.29528370468631726No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3840.28778919441509093No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.20984628759433713No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2710.20310122835023345No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2170.1626308728856113No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2160.16188142185848867No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1860.13939789104480968No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC1860.13939789104480968No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1840.1378989889905644No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1840.1378989889905644No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1800.13490118488207387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.13190338077358335No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA1700.12740667461084754No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1620.12141106639386649No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT1520.11391655612264016No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1400.10492314379716856No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTGT250.002058143571.3536762
TCTAATG300.004239239359.4613953
ATCGGGC300.004239239359.4613958
TTCCGGG350.00780123550.9669081
GGGATCG350.00780123550.9669085
TCGGGCA558.5284736E-443.244659
AGTATCA600.001308529439.640931
GTCGAGT650.001938938436.591631
GGCAGCG700.002789062233.977946
GGGCAAC350.002794044833.9779418-19
TATTGTA1251.2732653E-633.298385
CGGGCAA400.00537193329.73069610-11
CGTTTAT1755.6691715E-727.1823521
ATTGTAA1556.806502E-626.8535336
TAGCGTT450.00954625826.42728644-45
GTTTATT1608.709701E-626.0143592
TATTCTC1150.001003946625.852785
AGCGTCT1200.001286763624.7755819
TGGGGGG3052.7102942E-1023.3946486
TTATTGT1802.169294E-523.1238774
Produced by FastQC (version 0.11.5)