FastQCFastQC Report
Sun 1 Jan 2017
ERR1142699_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142699_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704184
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13970.1983856492053213No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13280.1885870738329755No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17800.050.5310551
GTATCAA27350.040.727311
TAACGAC650.001927799836.6562543
TATCAAC35050.031.270242
CAACGCA35450.030.7493745
ATCAACG35300.030.7112943
TCAACGC35700.030.7008954
AACGCAG36100.029.8678366
ACGCAGA40750.026.1672337
CGCAGAG41950.025.4187058
GCAGAGT44950.023.8547699
AGAGTAC42800.021.50389310-11
CAGAGTA44700.021.38946510-11
ATGGGTT3701.3278623E-1020.928745
GTACATG57700.020.5437011
CATGGGT12700.020.1681544
TACATGG58550.020.1437232
ACATGGG57900.020.0612283
GAGTACA47100.019.98336212-13
AGTACAT47350.019.06009312-13
Produced by FastQC (version 0.11.5)