##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142699_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	704184
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.193220805925723	33.0	33.0	33.0	27.0	33.0
2	31.327891573793213	33.0	33.0	33.0	27.0	33.0
3	31.377702418686024	33.0	33.0	33.0	27.0	33.0
4	31.34495955602513	33.0	33.0	33.0	27.0	33.0
5	31.352700430569282	33.0	33.0	33.0	27.0	33.0
6	34.60884087113595	37.0	37.0	37.0	27.0	37.0
7	34.60644661054497	37.0	37.0	37.0	27.0	37.0
8	34.5895192165684	37.0	37.0	37.0	27.0	37.0
9	34.578101746134536	37.0	37.0	37.0	27.0	37.0
10-11	34.606066028197176	37.0	37.0	37.0	27.0	37.0
12-13	34.571249843790824	37.0	37.0	37.0	27.0	37.0
14-15	34.57972561148791	37.0	37.0	37.0	27.0	37.0
16-17	34.60608377923952	37.0	37.0	37.0	27.0	37.0
18-19	34.56921486429683	37.0	37.0	37.0	27.0	37.0
20-21	34.59464713767993	37.0	37.0	37.0	27.0	37.0
22-23	34.66987960533042	37.0	37.0	37.0	27.0	37.0
24-25	34.70116617247765	37.0	37.0	37.0	27.0	37.0
26-27	34.762622411187984	37.0	37.0	37.0	27.0	37.0
28-29	34.77743601104257	37.0	37.0	37.0	27.0	37.0
30-31	34.80473569407996	37.0	37.0	37.0	27.0	37.0
32-33	34.80102643627234	37.0	37.0	37.0	27.0	37.0
34-35	34.79709351533122	37.0	37.0	37.0	27.0	37.0
36-37	34.780413925905734	37.0	37.0	37.0	27.0	37.0
38-39	34.74913303909206	37.0	37.0	37.0	27.0	37.0
40-41	34.716579757563366	37.0	37.0	37.0	27.0	37.0
42-43	34.70638568896766	37.0	37.0	37.0	27.0	37.0
44-45	34.710868892221356	37.0	37.0	37.0	27.0	37.0
46-47	34.69708272269747	37.0	37.0	37.0	27.0	37.0
48-49	34.66801006555105	37.0	37.0	37.0	27.0	37.0
50-51	34.64609533871829	37.0	37.0	37.0	27.0	37.0
52-53	34.61083395817002	37.0	37.0	37.0	27.0	37.0
54-55	34.58245004146644	37.0	37.0	37.0	27.0	37.0
56-57	34.54267634595503	37.0	37.0	37.0	27.0	37.0
58-59	34.51097369437534	37.0	37.0	37.0	27.0	37.0
60-61	34.484789486838665	37.0	37.0	37.0	27.0	37.0
62-63	34.42069189302796	37.0	37.0	37.0	27.0	37.0
64-65	34.39565937311839	37.0	37.0	37.0	27.0	37.0
66-67	34.35146211785556	37.0	37.0	37.0	27.0	37.0
68-69	34.31652593640298	37.0	37.0	37.0	27.0	37.0
70-71	34.28614254229008	37.0	37.0	37.0	27.0	37.0
72-73	34.23079550231189	37.0	37.0	37.0	27.0	37.0
74-75	34.18778543676085	37.0	37.0	37.0	27.0	37.0
76-77	34.1245079411063	37.0	37.0	37.0	27.0	37.0
78-79	34.080376009679284	37.0	37.0	37.0	27.0	37.0
80-81	34.016087414652986	37.0	37.0	37.0	27.0	37.0
82-83	33.97786587028391	37.0	37.0	37.0	27.0	37.0
84-85	33.92981237858287	37.0	37.0	37.0	27.0	37.0
86-87	33.837663877622894	37.0	35.0	37.0	27.0	37.0
88-89	33.76636078070504	37.0	33.0	37.0	22.0	37.0
90-91	33.684017955534344	37.0	33.0	37.0	22.0	37.0
92-93	33.63894876907172	37.0	33.0	37.0	22.0	37.0
94-95	33.566684275700666	37.0	33.0	37.0	22.0	37.0
96-97	33.452428626608956	37.0	33.0	37.0	22.0	37.0
98-99	33.35614555286686	37.0	33.0	37.0	22.0	37.0
100-101	33.28024777614941	37.0	33.0	37.0	18.0	37.0
102-103	33.213461254444866	37.0	33.0	37.0	18.0	37.0
104-105	33.015921264896676	37.0	33.0	37.0	14.0	37.0
106-107	32.92163837860559	37.0	33.0	37.0	14.0	37.0
108-109	32.82206710178022	37.0	33.0	37.0	14.0	37.0
110-111	32.70016714381468	37.0	33.0	37.0	14.0	37.0
112-113	32.60602129557048	37.0	33.0	37.0	14.0	37.0
114-115	32.45763394226509	37.0	33.0	37.0	14.0	37.0
116-117	32.366927535984914	37.0	33.0	37.0	14.0	37.0
118-119	32.23221984594935	37.0	33.0	37.0	14.0	37.0
120-121	32.105596690637675	37.0	33.0	37.0	14.0	37.0
122-123	31.971299404701043	37.0	33.0	37.0	14.0	37.0
124-125	30.345049731320223	35.0	24.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1277.0
1	75.0
2	35.0
3	18.0
4	23.0
5	18.0
6	18.0
7	14.0
8	23.0
9	23.0
10	26.0
11	32.0
12	20.0
13	24.0
14	38.0
15	202.0
16	301.0
17	588.0
18	1440.0
19	2733.0
20	3944.0
21	5101.0
22	5934.0
23	6880.0
24	7920.0
25	9190.0
26	10741.0
27	12760.0
28	15070.0
29	17976.0
30	21723.0
31	25776.0
32	32538.0
33	42388.0
34	62092.0
35	112520.0
36	304703.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.71618637911141	19.277930513810347	12.326320137889773	24.679562969188474
2	18.208654689618246	22.107935838868066	35.4296016131421	24.253807858371594
3	20.394943875422374	25.958275723112106	28.003622502232023	25.643157899233493
4	15.198742267336545	18.892335121956776	32.776809414766966	33.13211319593971
5	16.175343745638198	36.27111112377121	30.857776043343144	16.69576908724744
6	30.365249955113242	34.39722072599699	18.191248934830128	17.046280384059646
7	27.025551866155535	32.35510396032549	20.98658990181129	19.632754271707686
8	26.116983449932945	33.03272532418208	20.074847973959976	20.775443251925
9	26.533619493064048	17.87914026672139	19.567311666139382	36.01992857407518
10-11	25.939638538077197	26.364175309887617	24.962591170661216	22.733594981373965
12-13	26.56412566024075	25.387022937754228	24.703622095724576	23.345229306280444
14-15	24.877388719976	25.65783988958394	23.00567400042325	26.45909739001681
16-17	24.50717905044561	27.496930995098133	23.777453708582673	24.218436245873576
18-19	24.65409324477019	26.470045859834517	25.125721165655747	23.750139729739544
20-21	26.480636070022374	25.238636598229863	24.53808539734088	23.74264193440688
22-23	26.317269342171485	25.09696978756694	24.38791047102865	24.197850399232927
24-25	25.19557816471486	25.80457344350672	24.445651299666817	24.554197092111604
26-27	24.7408054907594	25.92977243558487	24.663216197227715	24.666205876428016
28-29	24.62952827442487	25.825443245817002	24.937777262171064	24.60725121758706
30-31	24.947937202943134	25.52265639123324	24.8584020030832	24.671004402740433
32-33	24.66418399129925	25.742382571163386	24.62026740008086	24.973166037456508
34-35	24.973381464958905	25.721547348252997	24.660649264502833	24.644421922285265
36-37	24.860578524395553	25.870334469202017	24.444235679941244	24.824851326461186
38-39	24.756832394067466	25.687865747851596	24.625586655639367	24.929715202441567
40-41	24.687113717893887	25.84273101137684	24.78318387899999	24.686971391729283
42-43	24.776391751403423	25.830666924767424	24.715187711367438	24.67775361246171
44-45	24.706856824874084	25.84854613224639	24.60246526329872	24.84213177958081
46-47	24.783081147896212	25.697580998100694	24.600551219036625	24.918786634966473
48-49	24.77109466070201	25.901708410251885	24.535392971364235	24.791803957681868
50-51	24.51546049845669	26.138764797021132	24.66335064411923	24.682424060402948
52-53	24.617822971614995	25.84007777338112	24.63661156295459	24.905487692049295
54-55	24.587180773651305	25.712750550651	24.76182342873206	24.938245246965636
56-57	24.626633057830066	25.641943486485065	24.58799048666867	25.1434329690162
58-59	24.544280933884263	25.712134431964884	24.73536482433804	25.008219809812815
60-61	24.537504252384537	25.740057248838866	24.826025874425483	24.89641262435111
62-63	24.29231166683154	25.867712189070836	24.77633312290985	25.06364302118777
64-65	24.459221227323873	25.548002678894875	24.747536932665366	25.245239161115883
66-67	24.60082046156836	25.600166284766935	24.644313609084158	25.154699644580546
68-69	24.624667589160133	25.39924890809801	24.56907596904522	25.407007533696635
70-71	24.361004796779277	25.49709908385004	24.750071357341195	25.391824762029497
72-73	24.551301854585784	25.54450963168812	24.83318408760558	25.07100442612052
74-75	24.524284602773815	25.465856195173757	24.901852889315688	25.108006312736737
76-77	24.530868406835303	25.338195422679327	24.861572102445542	25.269364068039827
78-79	24.527302662252477	25.395928286245706	24.85395373071965	25.22281532078217
80-81	24.358886753505587	25.509288917360667	24.94590362303367	25.185920706100077
82-83	24.435773681704397	25.4822676812503	24.985391219927443	25.09656741711786
84-85	24.422346651788256	25.567690126732177	24.777606663402526	25.23235655807704
86-87	24.19956807451604	25.65986046031729	24.94849982388541	25.19207164128126
88-89	24.439563348232774	25.322074081663725	24.884767706781307	25.353594863322193
90-91	24.633620981038305	25.317315006614212	24.840906648867474	25.208157363480012
92-93	24.499488762378032	25.505314096283442	24.88286361375624	25.11233352758228
94-95	23.987272965296633	25.61038255098498	25.115758527853195	25.286585955865192
96-97	24.14888917187121	25.496011490663122	24.98970236820632	25.365396969259347
98-99	24.050180817995997	25.715926141735977	24.871847957784706	25.362045082483313
100-101	24.215897481139137	25.92632381776322	24.720893368887474	25.136885332210174
102-103	24.29792377671582	25.828820964426807	24.882166164738994	24.991089094118372
104-105	24.183603188743202	25.90462515760144	24.89932007251801	25.012451581137345
106-107	24.110266987493723	25.864618002837357	24.938066243861766	25.087048765807157
108-109	24.102688164392138	25.92904612144412	24.847518891149978	25.12074682301376
110-111	23.980164630719212	26.228434016093118	24.789089123985285	25.00231222920239
112-113	24.026553803751995	26.22049389619657	24.723125158740153	25.029827141311284
114-115	23.917720429311938	26.238938871569257	24.8243096532579	25.019031045860906
116-117	23.940835180158867	26.296258088384388	24.77409440331824	24.988812328138508
118-119	23.790440483347147	26.071434923503233	24.84172408518691	25.29640050796271
120-121	23.79842462766158	26.063226916369796	24.97771270052344	25.16063575544518
122-123	23.75927824453088	26.175730467218706	25.047507315521855	25.01748397272856
124-125	23.872490103445063	26.109968995657113	24.73487622101393	25.282664679883897
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2096.0
1	1237.5
2	329.0
3	249.5
4	200.5
5	162.5
6	145.0
7	155.5
8	147.5
9	141.5
10	136.5
11	128.0
12	137.0
13	145.5
14	152.5
15	153.0
16	149.0
17	181.5
18	211.5
19	232.0
20	299.5
21	356.5
22	448.5
23	587.5
24	719.5
25	969.5
26	1293.0
27	1671.0
28	2169.0
29	2912.0
30	3864.0
31	4960.0
32	6206.0
33	7280.5
34	8315.0
35	9466.0
36	10574.0
37	11875.5
38	12864.5
39	13478.5
40	14068.0
41	14626.5
42	15575.5
43	17169.5
44	19241.0
45	21196.0
46	23349.0
47	25364.0
48	27186.5
49	28929.0
50	29356.5
51	29098.0
52	30049.5
53	31069.0
54	30882.5
55	30361.5
56	29437.5
57	27365.5
58	25000.5
59	22650.0
60	19758.5
61	16791.5
62	13974.5
63	10979.5
64	8429.5
65	6794.5
66	5405.0
67	4305.0
68	3557.0
69	2983.5
70	2526.0
71	2143.5
72	1827.5
73	1661.5
74	1388.5
75	1141.5
76	910.5
77	641.5
78	456.5
79	288.5
80	174.5
81	112.5
82	82.5
83	50.5
84	32.5
85	16.0
86	5.5
87	3.5
88	3.0
89	3.0
90	1.0
91	1.0
92	1.5
93	1.0
94	1.0
95	0.5
96	0.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3087261283982595
2	0.2772002772002772
3	0.270809901957443
4	0.2794724106199516
5	0.29338922781545734
6	0.3433761630482942
7	0.3513286300171546
8	0.35431080513047725
9	0.3518966633720732
10-11	0.3518966633720732
12-13	0.36155323040568943
14-15	0.3513996341865194
16-17	0.28430069413675974
18-19	0.274857139611238
20-21	0.2568220805925724
22-23	0.2519937970757643
24-25	0.2436153050907149
26-27	0.25099973870465675
28-29	0.23679890483169175
30-31	0.2367279006623269
32-33	0.24368630926007973
34-35	0.23658589232359722
36-37	0.232609658839167
38-39	0.2392840507594606
40-41	0.22330811265237493
42-43	0.22948547538711472
44-45	0.21883484998239097
46-47	0.22146200424888948
48-49	0.22735535030616996
50-51	0.23225463799234292
52-53	0.23147359212932983
54-55	0.22813639616918305
56-57	0.2259352669188735
58-59	0.2290594503709258
60-61	0.23246765050043738
62-63	0.23182861297615398
64-65	0.23509480476693592
66-67	0.2519937970757643
68-69	0.24709450938959135
70-71	0.24617145518784864
72-73	0.2503607011803733
74-75	0.2548339638503573
76-77	0.24744953023641547
78-79	0.248869613623712
80-81	0.24624245935721345
82-83	0.24077513831612193
84-85	0.22721334196744034
86-87	0.21535564568351453
88-89	0.20917828294877475
90-91	0.21734376242572964
92-93	0.2098173204730582
94-95	0.20278790770594052
96-97	0.1920662781318519
98-99	0.19959272008452333
100-101	0.1996637242538882
102-103	0.19788862001976756
104-105	0.20761619122274858
106-107	0.20122581597991435
108-109	0.2093202912875044
110-111	0.19866966588278065
112-113	0.1966105449712007
114-115	0.19668154914056554
116-117	0.19902468672960477
118-119	0.1954744782613635
120-121	0.20300092021403499
122-123	0.19845665337468615
124-125	0.21876384581302616
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	704184.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.41657630463725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.70736232654684	70.53125795011222
2	8.685072743360907	13.968476299556151
3	1.8589412146518571	4.484690641016583
4	0.6828049817493773	2.19635355664141
5	0.3514181875715693	1.4129923747843212
6	0.19620691394120665	0.9466972959870263
7	0.11697884590633253	0.6584926801498495
8	0.08262213841083443	0.5315351598373733
9	0.0609143616470966	0.4408671970297811
>10	0.24062395225388725	3.219928695988232
>50	0.012577592287635336	0.6824686729876499
>100	0.004050385322682984	0.45909111911408257
>500	0.0	0.0
>1k	4.263563497163309E-4	0.46714835679529526
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1397	0.1983856492053213	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1328	0.1885870738329755	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.420083387296502E-4	2.130125080944753E-4
14-15	0.0	0.0	0.0	2.840166774593004E-4	3.550208468241255E-4
16-17	0.0	0.0	0.0	2.840166774593004E-4	4.2602501618895064E-4
18-19	0.0	0.0	0.0	3.550208468241255E-4	5.680333549186008E-4
20-21	0.0	0.0	0.0	6.39037524283426E-4	5.680333549186008E-4
22-23	0.0	0.0	0.0	0.0014910875566613272	5.680333549186008E-4
24-25	0.0	0.0	0.0	0.004970291855537757	5.680333549186008E-4
26-27	0.0	0.0	0.0	0.008307487815684536	5.680333549186008E-4
28-29	0.0	0.0	0.0	0.015194892244072571	5.680333549186008E-4
30-31	0.0	0.0	0.0	0.029182713608943117	5.680333549186008E-4
32-33	0.0	0.0	0.0	0.05495722708837463	8.520500323779013E-4
34-35	0.0	0.0	0.0	0.08648307828635698	8.520500323779013E-4
36-37	0.0	0.0	0.0	0.13796110107585519	8.520500323779013E-4
38-39	0.0	0.0	0.0	0.2106693705054361	9.940583711075515E-4
40-41	0.0	0.0	0.0	0.28522374833850245	0.0011360667098372016
42-43	0.0	0.0	0.0	0.35210967588016767	0.0011360667098372016
44-45	0.0	0.0	0.0	0.418143553389455	0.001349079217931677
46-47	0.0	0.0	0.0	0.5008634106994763	0.001420083387296502
48-49	0.0	0.0	0.0	0.593523851720573	0.001420083387296502
50-51	0.0	0.0	0.0	0.6792968883132817	0.001420083387296502
52-53	0.0	0.0	0.0	0.7600286288810879	0.0016330958953909773
54-55	0.0	0.0	0.0	0.8438845529009463	0.0017041000647558026
56-57	0.0	0.0	0.0	0.9374680481237858	0.0017041000647558026
58-59	0.0	0.0	0.0	1.0406371062108768	0.0017041000647558026
60-61	0.0	0.0	0.0	1.1453682560239937	0.0017041000647558026
62-63	0.0	0.0	0.0	1.2454131306590321	0.0017041000647558026
64-65	0.0	0.0	0.0	1.3474461220362859	0.0017041000647558026
66-67	0.0	0.0	0.0	1.459916726310169	0.0017041000647558026
68-69	0.0	0.0	0.0	1.5721033139065925	0.0017041000647558026
70-71	0.0	0.0	0.0	1.7011178896424797	0.0017041000647558026
72-73	0.0	0.0	0.0	1.820049873328562	0.0017041000647558026
74-75	0.0	0.0	0.0	1.941822023789237	0.0017041000647558026
76-77	0.0	0.0	0.0	2.065653295161492	0.0017751042341206276
78-79	0.0	0.0	0.0	2.2116378673755723	0.0018461084034854527
80-81	0.0	0.0	0.0	2.363657793985663	0.0018461084034854527
82-83	0.0	0.0	0.0	2.523630187564614	0.0018461084034854527
84-85	0.0	0.0	0.0	2.6750820808197857	0.0018461084034854527
86-87	0.0	0.0	0.0	2.8251138906876614	0.002059120911579928
88-89	0.0	0.0	0.0	2.9917606761869058	0.0022721334196744033
90-91	0.0	0.0	0.0	3.1847500085205	0.0022721334196744033
92-93	0.0	0.0	0.0	3.3783783783783785	0.0022721334196744033
94-95	0.0	0.0	0.0	3.565190347977233	0.0022721334196744033
96-97	0.0	0.0	0.0	3.7578246594640037	0.0026271542664985286
98-99	0.0	0.0	0.0	3.9606835713393087	0.002698158435863354
100-101	0.0	0.0	0.0	4.180299467184714	0.002698158435863354
102-103	0.0	0.0	0.0	4.407583813321518	0.002769162605228179
104-105	0.0	0.0	0.0	4.649708030855572	0.002840166774593004
106-107	0.0	0.0	0.0	4.895098440180407	0.002840166774593004
108-109	0.0	0.0	0.0	5.136512616020813	0.002840166774593004
110-111	0.0	0.0	0.0	5.394257750815127	0.0029821751133226543
112-113	0.0	0.0	0.0	5.670889994660486	0.0029821751133226543
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1780	0.0	50.531055	1
GTATCAA	2735	0.0	40.72731	1
TAACGAC	65	0.0019277998	36.656254	3
TATCAAC	3505	0.0	31.27024	2
CAACGCA	3545	0.0	30.749374	5
ATCAACG	3530	0.0	30.711294	3
TCAACGC	3570	0.0	30.700895	4
AACGCAG	3610	0.0	29.867836	6
ACGCAGA	4075	0.0	26.167233	7
CGCAGAG	4195	0.0	25.418705	8
GCAGAGT	4495	0.0	23.854769	9
AGAGTAC	4280	0.0	21.503893	10-11
CAGAGTA	4470	0.0	21.389465	10-11
ATGGGTT	370	1.3278623E-10	20.92874	5
GTACATG	5770	0.0	20.543701	1
CATGGGT	1270	0.0	20.168154	4
TACATGG	5855	0.0	20.143723	2
ACATGGG	5790	0.0	20.061228	3
GAGTACA	4710	0.0	19.983362	12-13
AGTACAT	4735	0.0	19.060093	12-13
>>END_MODULE