FastQCFastQC Report
Sun 1 Jan 2017
ERR1142699_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142699_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704184
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37310.529833111800325No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31730.4505924587891801No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16740.23772195903343446No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9830.13959419697124614No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.063.115861
GTCAACG1950.057.970761
GTATCAA40400.041.235061
GCGCAGA3450.032.7660831
ATCAACG50900.032.604984
TATCAAC51550.032.4246373
CAACGCA51650.032.2466936
TCAACGC52550.031.6944185
AACGCAG54200.030.8393027
GTGGTAT11700.030.002411
TGGTATC12400.028.3026942
ACGCAGA59550.027.9687968
CGCAGAG62250.026.8512489
GAGTACT29950.026.01788312-13
CAGAGTA65750.025.1957310-11
GCAGAGT65550.025.1364849
GGACTAG1901.167773E-625.0510791
GTACTTT32000.024.2581714-15
GTACATG54600.022.9921631
CATGGGT12950.022.9666544
Produced by FastQC (version 0.11.5)