FastQCFastQC Report
Sun 1 Jan 2017
ERR1142688_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142688_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375688
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10960.2917314367240902No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7810.20788526649773215No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4230.1125934285896808No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCTA650.001929818336.6439935
TACGTAC350.002788645233.99931360-61
GGGTTAG750.003892236931.758131
CGATTCG400.005368555429.74146102-103
CTACGTA557.7496166E-427.04490960-61
GTAAGTA2601.0895747E-925.1927452
GTATTAA1951.4505058E-624.4293291
GGTATCA20750.023.2446541
CATGGGG16950.023.1862434
GTACATA1802.1589001E-523.1569671
ATGGGGG10850.022.5014365
ATAAGTA1350.002540546722.0542555
TGGGGGG6700.021.3329336
TAGGACT2055.887784E-520.3329474
GTACACT3551.7989805E-920.1310796
GACCGCG1500.00467527519.8514827
GCGATTC1057.157454E-519.82764102-103
GTATCAA24900.019.3705441
GCCTACA1550.00565557719.2085441
TGTAGTG1908.263887E-418.8041535
Produced by FastQC (version 0.11.5)