##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142688_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	375688
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.126629011307255	33.0	33.0	33.0	27.0	33.0
2	31.26691297033709	33.0	33.0	33.0	27.0	33.0
3	31.32694682821916	33.0	33.0	33.0	27.0	33.0
4	31.25550190583676	33.0	33.0	33.0	27.0	33.0
5	31.309237984710716	33.0	33.0	33.0	27.0	33.0
6	34.57651029577735	37.0	37.0	37.0	27.0	37.0
7	34.576789783011435	37.0	37.0	37.0	27.0	37.0
8	34.55425778837759	37.0	37.0	37.0	27.0	37.0
9	34.46448382700539	37.0	37.0	37.0	27.0	37.0
10-11	34.56164689849024	37.0	37.0	37.0	27.0	37.0
12-13	34.52248674431976	37.0	37.0	37.0	27.0	37.0
14-15	34.51406619322417	37.0	37.0	37.0	27.0	37.0
16-17	34.56281808309022	37.0	37.0	37.0	27.0	37.0
18-19	34.521776048210214	37.0	37.0	37.0	27.0	37.0
20-21	34.55516013287622	37.0	37.0	37.0	27.0	37.0
22-23	34.63599848810715	37.0	37.0	37.0	27.0	37.0
24-25	34.68519622665616	37.0	37.0	37.0	27.0	37.0
26-27	34.74874097655501	37.0	37.0	37.0	27.0	37.0
28-29	34.775565096569494	37.0	37.0	37.0	27.0	37.0
30-31	34.805406347820536	37.0	37.0	37.0	27.0	37.0
32-33	34.801518813483526	37.0	37.0	37.0	27.0	37.0
34-35	34.81370844956453	37.0	37.0	37.0	27.0	37.0
36-37	34.7992722684781	37.0	37.0	37.0	27.0	37.0
38-39	34.76323837865463	37.0	37.0	37.0	27.0	37.0
40-41	34.732931315346775	37.0	37.0	37.0	27.0	37.0
42-43	34.7317441600477	37.0	37.0	37.0	27.0	37.0
44-45	34.72498056898277	37.0	37.0	37.0	27.0	37.0
46-47	34.70938917399545	37.0	37.0	37.0	27.0	37.0
48-49	34.67632317241967	37.0	37.0	37.0	27.0	37.0
50-51	34.65147143374289	37.0	37.0	37.0	27.0	37.0
52-53	34.62094477332255	37.0	37.0	37.0	27.0	37.0
54-55	34.583080907561595	37.0	37.0	37.0	27.0	37.0
56-57	34.53448739379485	37.0	37.0	37.0	27.0	37.0
58-59	34.491377153382594	37.0	37.0	37.0	27.0	37.0
60-61	34.46501884542492	37.0	37.0	37.0	27.0	37.0
62-63	34.41814750537681	37.0	37.0	37.0	27.0	37.0
64-65	34.37949575179404	37.0	37.0	37.0	27.0	37.0
66-67	34.33044041864526	37.0	37.0	37.0	27.0	37.0
68-69	34.30416728774941	37.0	37.0	37.0	27.0	37.0
70-71	34.270258831796596	37.0	37.0	37.0	27.0	37.0
72-73	34.2191885287792	37.0	37.0	37.0	27.0	37.0
74-75	34.17729206149784	37.0	37.0	37.0	27.0	37.0
76-77	34.11187075445582	37.0	37.0	37.0	27.0	37.0
78-79	34.06286200251272	37.0	37.0	37.0	27.0	37.0
80-81	34.01152285939396	37.0	37.0	37.0	27.0	37.0
82-83	33.975532888992994	37.0	37.0	37.0	27.0	37.0
84-85	33.91833249930794	37.0	37.0	37.0	27.0	37.0
86-87	33.86104293988629	37.0	37.0	37.0	27.0	37.0
88-89	33.79605284171973	37.0	37.0	37.0	22.0	37.0
90-91	33.70156619322417	37.0	35.0	37.0	22.0	37.0
92-93	33.665447126338876	37.0	33.0	37.0	22.0	37.0
94-95	33.610671621132425	37.0	33.0	37.0	22.0	37.0
96-97	33.48508869061561	37.0	33.0	37.0	22.0	37.0
98-99	33.409681703967124	37.0	33.0	37.0	18.0	37.0
100-101	33.3432968314133	37.0	33.0	37.0	14.0	37.0
102-103	33.28117480462511	37.0	33.0	37.0	14.0	37.0
104-105	33.104864941121356	37.0	33.0	37.0	14.0	37.0
106-107	33.019405730286834	37.0	33.0	37.0	14.0	37.0
108-109	32.919379910989974	37.0	33.0	37.0	14.0	37.0
110-111	32.82066235812695	37.0	33.0	37.0	14.0	37.0
112-113	32.73293264623837	37.0	33.0	37.0	14.0	37.0
114-115	32.58888758757267	37.0	33.0	37.0	14.0	37.0
116-117	32.488993526543304	37.0	33.0	37.0	14.0	37.0
118-119	32.3570223163902	37.0	33.0	37.0	14.0	37.0
120-121	32.21903813802943	37.0	33.0	37.0	14.0	37.0
122-123	32.11647697025191	37.0	33.0	37.0	14.0	37.0
124-125	30.55952279551117	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	742.0
1	43.0
2	17.0
3	16.0
4	13.0
5	18.0
6	10.0
7	7.0
8	9.0
9	10.0
10	12.0
11	12.0
12	7.0
13	10.0
14	30.0
15	155.0
16	242.0
17	493.0
18	1066.0
19	1947.0
20	3071.0
21	3506.0
22	3606.0
23	3591.0
24	3847.0
25	4506.0
26	5253.0
27	6227.0
28	7506.0
29	8830.0
30	10474.0
31	12483.0
32	15706.0
33	20988.0
34	31261.0
35	58013.0
36	171961.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.521705577466896	19.933295771037336	12.255633676823619	24.289364974672154
2	16.87520522383079	23.12068362560766	36.37994506007886	23.62416609048269
3	19.564515224883014	27.97230237816223	28.13780474457913	24.325377652375625
4	14.361034731306226	19.368109132651696	34.13385301262714	32.13700312341493
5	15.058672754215227	38.20043520632251	31.085212329955812	15.655679709506455
6	29.28782447499025	36.39542275905912	18.37814591825287	15.938606847697756
7	25.979757074205363	33.73500412712066	21.664578619645095	18.620660179028885
8	25.135706958454435	34.65629808514276	20.626389126346385	19.58160583005642
9	26.439004583872038	18.70705959033647	19.84207545758583	35.01186036820567
10-11	24.729261164804754	27.767092475944715	25.799527709658975	21.704118649591557
12-13	25.438868332821286	26.64253369488265	25.303821647542847	22.614776324753215
14-15	24.12264474775577	26.769903525983075	23.593326557134443	25.51412516912671
16-17	23.674525827374335	28.696637642545454	24.299369816604752	23.329466713475462
18-19	23.70762491290733	27.99431928905689	25.66130362705919	22.636752170976585
20-21	25.277219860806206	26.812622194938047	25.206755503359734	22.703402440896017
22-23	25.199772603529446	26.60939051340358	24.749783813214616	23.44105306985235
24-25	24.355664084652695	27.085927023078	24.906093419533903	23.6523154727354
26-27	23.98296139223361	27.143938069571643	25.048007825375624	23.825092712819124
28-29	23.956187355229872	27.128321040103327	25.38627605862323	23.529215546043574
30-31	24.555911813486297	26.861714547999416	25.25828699978785	23.32408663872644
32-33	23.866450676259916	27.20171440161406	25.217102383722796	23.714732538403236
34-35	23.987772545869632	27.281072664221355	25.263756219469723	23.467398570439286
36-37	24.05638034853452	27.3828155750446	24.96220718070238	23.598596895718504
38-39	23.93656766205001	27.18130387212126	25.1276919381955	23.75443652763323
40-41	23.57635720346445	27.257736580055393	25.336599266765923	23.82930694971423
42-43	23.776385184671277	27.199371331823922	25.247591461746904	23.776652021757897
44-45	23.886150336519542	27.221062321151035	25.213951719951705	23.67883562237772
46-47	23.715228351225836	27.073825091485908	25.38085219639553	23.830094360892726
48-49	23.776112752121975	27.309756143117493	25.2854424992462	23.62868860551433
50-51	23.472024869747365	27.52376568535233	25.353533331999117	23.65067611290118
52-53	23.34489194983943	27.342287592076453	25.221310370867485	24.091510087216633
54-55	23.37648476180438	27.020096567053265	25.26886813220534	24.334550538937016
56-57	23.62006078216345	26.87662928483568	24.918285612130816	24.585024320870055
58-59	23.65450375507492	26.87870487606051	25.31089939014221	24.155891978722355
60-61	23.6906918846512	27.0838058592322	25.398322655359845	23.827179600756754
62-63	23.180559187529017	27.004360147081584	25.47470634382722	24.34037432156218
64-65	23.07553748053622	26.650201676120027	25.456156291988062	24.818104551355695
66-67	23.537112309741197	26.499318097505387	25.3862557281144	24.57731386463902
68-69	23.538085242944128	26.416745951153644	25.20459400782748	24.840574798074755
70-71	23.256325984876085	26.542515502215746	25.50036095109613	24.700797561812042
72-73	23.54155490970037	26.679023317507212	25.464041612289396	24.315380160503018
74-75	23.590443667788946	26.349305480492202	25.651783438555476	24.408467413163375
76-77	23.404388756563453	26.195899468251906	25.65921418706591	24.740497588118732
78-79	23.41235528654465	26.34692265402742	25.69227607777831	24.548445981649614
80-81	23.271267723659033	26.50976352442282	25.711414930439723	24.50755382147842
82-83	23.451645658123148	26.297039056276745	25.932908759275048	24.31840652632506
84-85	23.2604896271096	26.612634247214995	25.508371689680477	24.618504435994932
86-87	23.0198581143996	26.75416011888722	25.65960262426996	24.566379142443218
88-89	23.350718992609984	26.10049889283142	25.651895525971774	24.896886588586824
90-91	23.583964312965897	26.241949340206077	25.62604385108347	24.548042495744557
92-93	23.384973281862344	26.46256125963414	25.612996513169655	24.539468945333866
94-95	22.80077416910873	26.636272570484206	25.789408395546527	24.77354486486054
96-97	22.84945340287882	26.67596666404382	25.5949086515315	24.87967128154586
98-99	22.841072931435303	26.834889611195063	25.426437028431803	24.89760042893783
100-101	22.99129293511316	27.175165658311727	25.204471974134897	24.629069432440218
102-103	23.075076292707912	27.07270748415459	25.54351885443565	24.30869736870185
104-105	23.096972469844083	27.061960524859042	25.477165803862146	24.363901201434732
106-107	23.014766787200074	27.13828974167793	25.574912334288797	24.272031136833203
108-109	22.943391494918586	27.17152220975786	25.42454044522224	24.460545850101308
110-111	22.691770078639337	27.681153234669598	25.302766781549096	24.324309905141966
112-113	22.675499550531228	27.75382982071931	25.099563335084973	24.471107293664492
114-115	22.6434773330488	27.813596169491976	25.144510983368235	24.39841551409099
116-117	22.723738211054634	27.615508656897177	25.239780246455812	24.42097288559238
118-119	22.528178228042588	27.280632611389194	25.49691176490202	24.6942773956662
120-121	22.7359061843927	27.44495989201388	25.589482024099468	24.22965189949395
122-123	22.641154604855135	27.570578969621067	25.520864593211456	24.267401832312345
124-125	22.668713988983413	27.57308260305204	25.21875087547042	24.539452532494128
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1416.0
1	886.5
2	293.0
3	191.0
4	138.0
5	122.5
6	128.0
7	119.5
8	96.5
9	98.0
10	97.0
11	89.0
12	97.0
13	104.5
14	101.0
15	106.0
16	125.5
17	145.0
18	165.0
19	195.0
20	223.0
21	259.0
22	350.0
23	429.0
24	555.0
25	739.5
26	930.0
27	1201.0
28	1501.5
29	2040.5
30	2772.0
31	3336.0
32	4133.0
33	4985.5
34	5603.0
35	6376.5
36	7079.5
37	7887.0
38	8659.0
39	9057.5
40	9393.5
41	9642.5
42	10000.0
43	10650.0
44	11611.0
45	12575.5
46	13478.0
47	14490.0
48	15530.5
49	16218.5
50	16694.5
51	16522.5
52	15746.5
53	15392.5
54	14885.5
55	14246.5
56	13498.5
57	12078.0
58	10616.0
59	9309.0
60	7965.5
61	6689.0
62	5494.0
63	4324.0
64	3276.0
65	2544.0
66	1979.5
67	1586.5
68	1299.0
69	1078.5
70	918.5
71	742.5
72	646.0
73	593.0
74	460.5
75	393.0
76	318.0
77	237.5
78	173.0
79	95.0
80	60.0
81	41.5
82	28.0
83	11.5
84	6.0
85	9.0
86	7.5
87	3.0
88	1.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3191478034965184
2	0.2919976150422691
3	0.28507697876961735
4	0.292263793360448
5	0.30690360086028834
6	0.34975831008709357
7	0.35534805476885073
8	0.3598730861778923
9	0.354549519814314
10-11	0.35721130299610315
12-13	0.3654628308596495
14-15	0.3562796788824769
16-17	0.2966557356104001
18-19	0.28933583186047995
20-21	0.2742967568833713
22-23	0.2693724579970614
24-25	0.26098784097442557
26-27	0.2687070122016141
28-29	0.2544664721790422
30-31	0.25539809629266835
32-33	0.26085475181533613
34-35	0.25486573965631054
36-37	0.2522039564745214
38-39	0.25499882881540004
40-41	0.24222226954281212
42-43	0.24688039011094312
44-45	0.23836268392921786
46-47	0.2404921104746492
48-49	0.24461787440642235
50-51	0.24900981665637442
52-53	0.24794510338365877
54-55	0.24554949852004854
56-57	0.2414237345882754
58-59	0.24714656842912205
60-61	0.24727965758821147
62-63	0.24754583590639043
64-65	0.2538010263835949
66-67	0.26644449649709334
68-69	0.2615201976107834
70-71	0.2617863759289623
72-73	0.2637827133153042
74-75	0.2673761206107195
76-77	0.26098784097442557
78-79	0.261387108451694
80-81	0.2600562168607994
82-83	0.25539809629266835
84-85	0.24302080449734886
86-87	0.23397074167926576
88-89	0.22838099699750858
90-91	0.2326398500883712
92-93	0.22598539213389834
94-95	0.22212580652030406
96-97	0.20695364238410596
98-99	0.2152051702476523
100-101	0.21826622090670983
102-103	0.21720150763399415
104-105	0.22478758970209323
106-107	0.21999637997487279
108-109	0.22398905474755648
110-111	0.2161367943612785
112-113	0.21320883286131045
114-115	0.21374118949766827
116-117	0.2165360618385469
118-119	0.21227720874768424
120-121	0.21959711249760439
122-123	0.21467281361129448
124-125	0.2367656140201444
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	375688.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.38507603922325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.350219144641	67.59603350550859
2	8.679027593023969	13.432544204653531
3	1.9775817140919631	4.591059339563521
4	0.7192457065071266	2.2263553475575537
5	0.3449162213684088	1.334568400887794
6	0.23408688423771526	1.0868898801912268
7	0.13009977894845165	0.7047446900628448
8	0.08372253050393108	0.5183099511394316
9	0.06692532869419525	0.4661119484955279
>10	0.3831539657515788	5.35504140580754
>50	0.024041377479722636	1.2861068114711511
>100	0.006204226446154552	0.8390001504371518
>500	3.877641528846595E-4	0.23435590754342472
>1k	3.877641528846595E-4	0.32887845668065746
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1096	0.2917314367240902	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	781	0.20788526649773215	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	423	0.1125934285896808	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.661783181789144E-4	0.0
2	0.0	0.0	0.0	2.661783181789144E-4	0.0
3	0.0	0.0	0.0	2.661783181789144E-4	0.0
4	0.0	0.0	0.0	2.661783181789144E-4	0.0
5	0.0	0.0	0.0	2.661783181789144E-4	0.0
6	0.0	0.0	0.0	2.661783181789144E-4	0.0
7	0.0	0.0	0.0	2.661783181789144E-4	0.0
8	0.0	0.0	0.0	2.661783181789144E-4	0.0
9	0.0	0.0	0.0	2.661783181789144E-4	0.0
10-11	0.0	0.0	0.0	2.661783181789144E-4	0.0
12-13	0.0	0.0	0.0	5.323566363578288E-4	0.0
14-15	0.0	0.0	0.0	6.65445795447286E-4	0.0
16-17	0.0	0.0	0.0	7.985349545367432E-4	0.0
18-19	0.0	0.0	0.0	0.0014639807499840292	0.0
20-21	0.0	0.0	0.0	0.0021294265454313154	0.0
22-23	0.0	0.0	0.0	0.0041257639317731735	0.0
24-25	0.0	0.0	0.0	0.008251527863546347	0.0
26-27	0.0	0.0	0.0	0.013442005068035178	0.0
28-29	0.0	0.0	0.0	0.027017099295159816	0.0
30-31	1.330891590894572E-4	0.0	0.0	0.051239326249441025	0.0
32-33	2.661783181789144E-4	0.0	0.0	0.0830476352718213	0.0
34-35	2.661783181789144E-4	0.0	0.0	0.11818317327143801	0.0
36-37	2.661783181789144E-4	0.0	0.0	0.17833947317987264	0.0
38-39	2.661783181789144E-4	0.0	0.0	0.2665775856561828	0.0
40-41	2.661783181789144E-4	0.0	0.0	0.36306722599603924	0.0
42-43	2.661783181789144E-4	0.0	0.0	0.44092438406337175	0.0
44-45	2.661783181789144E-4	0.0	0.0	0.5098645684717106	0.0
46-47	2.661783181789144E-4	0.0	0.0	0.6060880304933881	0.0
48-49	2.661783181789144E-4	0.0	0.0	0.7052394540150337	0.0
50-51	2.661783181789144E-4	0.0	0.0	0.7989342220140117	0.0
52-53	2.661783181789144E-4	0.0	0.0	0.8979525563765678	0.0
54-55	2.661783181789144E-4	0.0	0.0	0.9968378015800345	0.0
56-57	2.661783181789144E-4	0.0	0.0	1.1029098613743318	0.0
58-59	2.661783181789144E-4	0.0	0.0	1.219096697259428	1.330891590894572E-4
60-61	2.661783181789144E-4	0.0	0.0	1.337546048849045	2.661783181789144E-4
62-63	2.661783181789144E-4	0.0	0.0	1.4553299546432146	5.323566363578288E-4
64-65	2.661783181789144E-4	0.0	0.0	1.557941696301186	5.323566363578288E-4
66-67	2.661783181789144E-4	0.0	0.0	1.6698696790954197	5.323566363578288E-4
68-69	2.661783181789144E-4	0.0	0.0	1.8080162262302761	5.323566363578288E-4
70-71	2.661783181789144E-4	0.0	0.0	1.961068759183152	5.323566363578288E-4
72-73	2.661783181789144E-4	0.0	0.0	2.099614573795277	9.316241136262005E-4
74-75	2.661783181789144E-4	0.0	0.0	2.230840484657482	0.0010647132727156577
76-77	2.661783181789144E-4	0.0	0.0	2.3806988777922102	0.0010647132727156577
78-79	2.661783181789144E-4	0.0	0.0	2.556642746108473	0.0010647132727156577
80-81	2.661783181789144E-4	0.0	0.0	2.739374161538298	0.001330891590894572
82-83	2.661783181789144E-4	0.0	0.0	2.9279614999680588	0.0015970699090734864
84-85	2.661783181789144E-4	0.0	0.0	3.103373011647963	0.0015970699090734864
86-87	2.661783181789144E-4	0.0	0.0	3.2676050339643536	0.0015970699090734864
88-89	2.661783181789144E-4	0.0	0.0	3.4571239965077405	0.0015970699090734864
90-91	2.661783181789144E-4	0.0	0.0	3.6558261110283	0.0015970699090734864
92-93	2.661783181789144E-4	0.0	0.0	3.87156363791231	0.0015970699090734864
94-95	2.661783181789144E-4	0.0	0.0	4.060816422137519	0.0015970699090734864
96-97	2.661783181789144E-4	0.0	0.0	4.269500223589787	0.0015970699090734864
98-99	2.661783181789144E-4	0.0	0.0	4.492424565064628	0.0015970699090734864
100-101	2.661783181789144E-4	0.0	0.0	4.747822661357296	0.0015970699090734864
102-103	2.661783181789144E-4	0.0	0.0	5.019590724217968	0.0015970699090734864
104-105	2.661783181789144E-4	0.0	0.0	5.302937543919423	0.0015970699090734864
106-107	2.661783181789144E-4	0.0	0.0	5.5852196503481615	0.0015970699090734864
108-109	2.661783181789144E-4	0.0	0.0	5.868433380890526	0.0015970699090734864
110-111	2.661783181789144E-4	0.0	0.0	6.154841251251038	0.0015970699090734864
112-113	2.661783181789144E-4	0.0	0.0	6.458683801452269	0.0015970699090734864
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCTA	65	0.0019298183	36.643993	5
TACGTAC	35	0.0027886452	33.999313	60-61
GGGTTAG	75	0.0038922369	31.75813	1
CGATTCG	40	0.0053685554	29.74146	102-103
CTACGTA	55	7.7496166E-4	27.044909	60-61
GTAAGTA	260	1.0895747E-9	25.192745	2
GTATTAA	195	1.4505058E-6	24.429329	1
GGTATCA	2075	0.0	23.244654	1
CATGGGG	1695	0.0	23.186243	4
GTACATA	180	2.1589001E-5	23.156967	1
ATGGGGG	1085	0.0	22.501436	5
ATAAGTA	135	0.0025405467	22.054255	5
TGGGGGG	670	0.0	21.332933	6
TAGGACT	205	5.887784E-5	20.332947	4
GTACACT	355	1.7989805E-9	20.131079	6
GACCGCG	150	0.004675275	19.851482	7
GCGATTC	105	7.157454E-5	19.82764	102-103
GTATCAA	2490	0.0	19.370544	1
GCCTACA	155	0.005655577	19.208544	1
TGTAGTG	190	8.263887E-4	18.804153	5
>>END_MODULE