FastQCFastQC Report
Sun 1 Jan 2017
ERR1142688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375688
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20910.55657886331211No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19180.5105300142671578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10560.2810843039969336No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5200.13841272545303548No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.13601712058942528No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.13575094227124634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG752.3072062E-855.5327151
CGTTAAC558.5215364E-443.272241
GCGCAGA2450.036.4281651
GTATCAA27350.035.8953931
GGTATCA19050.033.7319071
CATGGGG14800.030.545584
TATCAAC32900.029.6513462
ATCAACG32750.029.4238993
CAACGCA33400.029.2074645
TCAACGC33950.029.0847154
TGATCGC1055.907767E-428.3254769
AACGCAG35300.028.3094256
ACCTACT850.00721405327.9922318
ATGGGGG10200.027.4090615
ACGCAGA36850.026.63447
TCGACGG450.00953979226.437109114-115
CGCAGAG38500.025.4929268
GTATAAG2151.227545E-724.9066981
GTACTAG1200.001286332624.791391
GTGATCG1200.001288330624.784798
Produced by FastQC (version 0.11.5)