##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142688_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	375688
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.825871467813716	27.0	14.0	33.0	14.0	33.0
2	28.29982591937991	27.0	27.0	33.0	14.0	33.0
3	31.078488000681418	33.0	33.0	33.0	27.0	33.0
4	30.63504556972807	33.0	33.0	33.0	27.0	33.0
5	31.950099550691	33.0	33.0	33.0	33.0	33.0
6	34.88804806115713	37.0	37.0	37.0	27.0	37.0
7	35.53125998168693	37.0	37.0	37.0	33.0	37.0
8	35.37331509124593	37.0	37.0	37.0	33.0	37.0
9	35.818817742381974	37.0	37.0	37.0	33.0	37.0
10-11	35.9210861140095	37.0	37.0	37.0	33.0	37.0
12-13	35.98946998573284	37.0	37.0	37.0	33.0	37.0
14-15	35.97386395093801	37.0	37.0	37.0	33.0	37.0
16-17	35.94572757181491	37.0	37.0	37.0	33.0	37.0
18-19	35.93993020804497	37.0	37.0	37.0	33.0	37.0
20-21	35.95918288579885	37.0	37.0	37.0	33.0	37.0
22-23	35.970737686591	37.0	37.0	37.0	33.0	37.0
24-25	35.95023530163327	37.0	37.0	37.0	33.0	37.0
26-27	35.8276428845212	37.0	37.0	37.0	33.0	37.0
28-29	35.78674724771619	37.0	37.0	37.0	33.0	37.0
30-31	35.797337152104944	37.0	37.0	37.0	33.0	37.0
32-33	35.73699319648219	37.0	37.0	37.0	33.0	37.0
34-35	35.65825764996487	37.0	37.0	37.0	33.0	37.0
36-37	35.57295814612125	37.0	37.0	37.0	33.0	37.0
38-39	35.50574545899789	37.0	37.0	37.0	33.0	37.0
40-41	35.43598677626115	37.0	37.0	37.0	33.0	37.0
42-43	35.34750244884053	37.0	37.0	37.0	33.0	37.0
44-45	35.23442723749494	37.0	37.0	37.0	33.0	37.0
46-47	35.07497843955623	37.0	37.0	37.0	33.0	37.0
48-49	34.93800174612977	37.0	37.0	37.0	27.0	37.0
50-51	34.8389847426588	37.0	37.0	37.0	27.0	37.0
52-53	34.751859255552475	37.0	37.0	37.0	27.0	37.0
54-55	34.67942681160963	37.0	37.0	37.0	27.0	37.0
56-57	34.6285667894636	37.0	37.0	37.0	27.0	37.0
58-59	34.563499499584765	37.0	37.0	37.0	27.0	37.0
60-61	34.53166457273056	37.0	37.0	37.0	27.0	37.0
62-63	34.509096644023764	37.0	37.0	37.0	27.0	37.0
64-65	34.49455266071847	37.0	37.0	37.0	27.0	37.0
66-67	34.47612114307617	37.0	37.0	37.0	27.0	37.0
68-69	34.42868417410192	37.0	37.0	37.0	27.0	37.0
70-71	34.37202412640276	37.0	37.0	37.0	27.0	37.0
72-73	34.341208662507185	37.0	37.0	37.0	27.0	37.0
74-75	34.258974201997404	37.0	37.0	37.0	27.0	37.0
76-77	34.184803613636845	37.0	37.0	37.0	27.0	37.0
78-79	34.16201608781755	37.0	37.0	37.0	27.0	37.0
80-81	34.12464199016205	37.0	37.0	37.0	27.0	37.0
82-83	34.070744873405594	37.0	37.0	37.0	27.0	37.0
84-85	33.97492334064437	37.0	37.0	37.0	27.0	37.0
86-87	33.84943357253891	37.0	37.0	37.0	24.5	37.0
88-89	33.78629607546688	37.0	37.0	37.0	22.0	37.0
90-91	33.72259694214348	37.0	33.0	37.0	22.0	37.0
92-93	33.60093748003662	37.0	33.0	37.0	22.0	37.0
94-95	33.62659973169225	37.0	33.0	37.0	22.0	37.0
96-97	33.59672387725985	37.0	33.0	37.0	22.0	37.0
98-99	33.52355411937565	37.0	33.0	37.0	22.0	37.0
100-101	33.42568035178127	37.0	33.0	37.0	14.0	37.0
102-103	33.31302170950363	37.0	33.0	37.0	14.0	37.0
104-105	33.21287211728881	37.0	33.0	37.0	14.0	37.0
106-107	33.11665664061668	37.0	33.0	37.0	14.0	37.0
108-109	32.98781169481059	37.0	33.0	37.0	14.0	37.0
110-111	32.92745443027192	37.0	33.0	37.0	14.0	37.0
112-113	32.853565990928644	37.0	33.0	37.0	14.0	37.0
114-115	32.74289436979622	37.0	33.0	37.0	14.0	37.0
116-117	32.65314835714742	37.0	33.0	37.0	14.0	37.0
118-119	32.53847208321799	37.0	33.0	37.0	14.0	37.0
120-121	32.43146706841847	37.0	33.0	37.0	14.0	37.0
122-123	32.308354272694366	37.0	33.0	37.0	14.0	37.0
124-125	31.03533250995507	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	8.0
15	166.0
16	57.0
17	33.0
18	112.0
19	395.0
20	1181.0
21	2694.0
22	4010.0
23	5202.0
24	4625.0
25	3655.0
26	3624.0
27	4301.0
28	5511.0
29	6795.0
30	8949.0
31	11948.0
32	16369.0
33	23960.0
34	38438.0
35	75812.0
36	157843.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.54268429068987	13.372458380819497	12.347822382225441	21.73703494626519
2	18.13180085602947	20.601935648729796	38.51254232235259	22.75372117288814
3	20.421732927322672	26.96865484125125	28.80342198845851	23.806190242967567
4	14.467667147564722	18.52797282837263	34.63530714479645	32.3690528792662
5	16.02212591969505	35.37272272325564	32.5148801601414	16.090271196907906
6	29.677975756349717	34.33106370745783	19.130337569140185	16.860622967052272
7	26.04208811567045	32.256020953557204	22.90145013947744	18.800440791294903
8	26.185558229168887	32.03456059283235	21.35921296394881	20.420668214049957
9	27.15098698920381	16.091277868869913	21.158514512041908	35.59922062988437
10-11	25.35734439215519	25.227316283724793	26.921674368092674	22.49366495602734
12-13	26.650571751027446	24.0898032409872	27.124768424863184	22.134856583122165
14-15	24.570122016141053	24.29036860373501	26.279918442963314	24.859590937160622
16-17	23.304044845723045	26.534917271778706	26.816134664934733	23.34490321756351
18-19	23.07779327527097	25.111528715316965	29.468335427269437	22.34234258214263
20-21	24.327629988873685	24.17058394510309	29.407936414984853	22.093849651038365
22-23	24.323108536871022	23.907338003875555	29.28986286493047	22.47969059432295
24-25	23.509800685675348	24.520479759800686	29.28640254679415	22.68331700772982
26-27	23.00406188113541	24.65356891889014	29.48750026617832	22.85486893379613
28-29	23.018967866953428	24.58023679223185	29.69232448201699	22.708470858797725
30-31	23.6025638295607	24.2324748195311	29.728790911607504	22.436170439300696
32-33	23.06528289431656	24.56865803539107	29.451699282383252	22.914359787909117
34-35	23.21014567978131	24.587089784847493	29.511401778608253	22.691362756762945
36-37	23.362679922540973	24.76146746255149	29.068694975145238	22.8071576397623
38-39	23.265093176960363	24.64258430644985	29.207203945879908	22.88511857070988
40-41	22.985528734240056	24.58451509347828	29.193412932171515	23.236543240110148
42-43	23.32315186360823	24.62491598623838	29.033958196083127	23.017973954070257
44-45	23.227478229853883	24.748826481654252	28.92424934087331	23.09944594761856
46-47	23.265377827819435	24.617034925350094	28.632079363938594	23.485507882891877
48-49	23.240561316837375	24.946764336364215	28.482544025893823	23.33013032090458
50-51	23.089238942952665	25.209881603884078	28.209311982283168	23.491567470880092
52-53	23.064218181043845	25.17807329486169	28.009944421967166	23.7477641021273
54-55	22.98951386328761	25.10976572221786	27.922616330371202	23.97810408412333
56-57	23.228184025042058	24.915488383978197	27.692526777538806	24.163800813440943
58-59	23.21400527566831	24.957877169026343	27.963836917433927	23.864280637871417
60-61	23.27157109090522	25.02608547518153	28.311923723941142	23.390419709972104
62-63	22.808686995592087	25.09116607397628	28.430373075530756	23.669773854900875
64-65	22.789788335001386	24.733954770980173	28.524866378484276	23.951390515534165
66-67	22.995574779570788	24.733588421227747	28.373182498752286	23.897654300449176
68-69	23.002730989544514	24.652504205617426	28.505568450416302	23.839196354421755
70-71	22.879406949971383	24.784327295473602	28.67415520981674	23.662110544738276
72-73	22.95481889227231	24.98536019250016	28.749387789868187	23.31043312535934
74-75	22.9672750324959	24.85922883504869	28.876068102878815	23.297428029576594
76-77	22.916183885634833	24.7000098553921	28.745515130690492	23.638291128282578
78-79	22.819558328553196	24.90653776486512	28.742049554615352	23.531854351966334
80-81	22.553954345094866	25.140542151998467	28.884606375503076	23.420897127403588
82-83	22.744465472587553	24.88560956328007	28.994881377316755	23.375043586815618
84-85	22.80120834976078	25.019046959412663	28.4464340895287	23.73331060129786
86-87	22.359664824275317	25.285188586325607	28.60615786099564	23.748988728403436
88-89	22.736369151184263	24.710250718290236	28.564152817497618	23.989227313027886
90-91	23.025456288828664	24.693332623069804	28.419153143914926	23.862057944186603
92-93	22.866390309096747	24.9811786370535	28.330381877126147	23.822049176723603
94-95	22.349501176520693	25.087839780240838	28.44305320428871	24.119605838949756
96-97	22.48650806854101	25.022126102146064	28.17248377973715	24.318882049575777
98-99	22.41035114243734	25.168357786248162	27.93541449287707	24.485876578437427
100-101	22.650873398768923	25.38066877391449	27.64691399101647	24.321543836300116
102-103	22.797271668607554	25.319980036599567	27.811146231908168	24.07160206288471
104-105	22.85007739495116	25.389004460025475	27.577890731372307	24.18302741365106
106-107	22.793378772088836	25.37143587265969	27.567243102665767	24.267942252585712
108-109	22.748042427057698	25.28866131692118	27.439163171674124	24.524133084347003
110-111	22.53480073840519	25.88770331763268	27.323520760658493	24.25397518330363
112-113	22.485941877566233	25.842322767836347	27.17766139390027	24.49407396069715
114-115	22.623067330177264	25.93201367658783	27.160990048671934	24.283928944562973
116-117	22.575042522099515	25.777309302690803	27.162105689044335	24.485542486165347
118-119	22.449911228349436	25.494632442777554	27.452266937817253	24.603189391055757
120-121	22.497258363322757	25.638428696152125	27.57181491024467	24.292498030280445
122-123	22.400236366346544	25.910862204808243	27.44950597304146	24.239395455803752
124-125	22.30439782158505	25.862688521797462	27.30564886634051	24.527264790276988
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	167.0
1	150.0
2	296.5
3	406.0
4	286.0
5	252.0
6	342.5
7	644.5
8	1092.5
9	1341.0
10	1167.0
11	890.5
12	817.5
13	775.0
14	762.0
15	773.5
16	792.5
17	786.5
18	733.0
19	711.5
20	717.0
21	713.0
22	717.0
23	774.0
24	880.0
25	962.5
26	1103.5
27	1329.0
28	1504.5
29	1939.0
30	2600.5
31	3125.5
32	3807.0
33	4412.0
34	5002.5
35	5709.0
36	6430.5
37	7344.0
38	7994.0
39	8460.5
40	8791.5
41	8991.0
42	9311.5
43	10067.5
44	11016.0
45	11779.5
46	12931.0
47	14120.0
48	15057.5
49	15915.0
50	16726.0
51	16608.0
52	15469.5
53	14914.5
54	14735.0
55	14232.5
56	13434.0
57	12127.0
58	10698.0
59	9305.5
60	7983.0
61	6727.5
62	5454.0
63	4274.5
64	3297.0
65	2541.0
66	1922.0
67	1526.0
68	1272.5
69	1079.0
70	898.0
71	751.0
72	622.0
73	578.5
74	501.5
75	417.0
76	320.5
77	202.5
78	142.0
79	99.0
80	73.0
81	45.5
82	31.0
83	23.5
84	13.5
85	8.5
86	7.5
87	4.5
88	0.5
89	2.0
90	2.5
91	2.5
92	2.5
93	1.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03726496454504802
2	0.0
3	0.0
4	5.323566363578288E-4
5	0.005323566363578288
6	5.323566363578288E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.323566363578288E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	2.661783181789144E-4
36-37	0.0014639807499840292
38-39	0.001330891590894572
40-41	0.0033272289772364304
42-43	0.0014639807499840292
44-45	3.992674772683716E-4
46-47	2.661783181789144E-4
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.992674772683716E-4
56-57	0.0
58-59	2.661783181789144E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.330891590894572E-4
68-69	0.0
70-71	7.985349545367432E-4
72-73	0.0
74-75	0.06814164945380209
76-77	0.06894018440833884
78-79	3.992674772683716E-4
80-81	0.0
82-83	2.661783181789144E-4
84-85	0.07985349545367432
86-87	0.14413555929388217
88-89	0.17714167074806755
90-91	0.06401588552202891
92-93	0.11964715402142204
94-95	0.0010647132727156577
96-97	1.330891590894572E-4
98-99	0.0
100-101	1.330891590894572E-4
102-103	1.330891590894572E-4
104-105	0.004658120568131002
106-107	0.004658120568131002
108-109	0.008384617022635805
110-111	0.0035934072954153444
112-113	0.0041257639317731735
114-115	0.002528694022699687
116-117	2.661783181789144E-4
118-119	0.001330891590894572
120-121	0.0
122-123	0.0
124-125	5.323566363578288E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	375688.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.0807587425199
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.00989203914115	65.32768712403461
2	8.877166138771415	13.330087383647271
3	1.9904321567829304	4.483294696703183
4	0.7526259809247666	2.2603091878865915
5	0.3764469156368097	1.4131960026146526
6	0.21316429307863208	0.9602722116693947
7	0.1471881648188988	0.7735699364765397
8	0.10170146651685388	0.6108658617049898
9	0.07693794614255776	0.5198903435226933
>10	0.41477274190694235	5.660326040121948
>50	0.02710276023051622	1.4167593381628878
>100	0.010212634289432098	1.2955503273105584
>500	0.0011783808795498573	0.45446006245972476
>1k	0.0011783808795498573	1.4937314836849485
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2091	0.55657886331211	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1918	0.5105300142671578	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1056	0.2810843039969336	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	520	0.13841272545303548	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	511	0.13601712058942528	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	510	0.13575094227124634	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.661783181789144E-4	2.661783181789144E-4
14-15	0.0	0.0	0.0	3.9926747726837166E-4	3.9926747726837166E-4
16-17	0.0	0.0	0.0	7.985349545367432E-4	5.323566363578288E-4
18-19	0.0	0.0	0.0	0.0011978024318051149	5.323566363578288E-4
20-21	0.0	0.0	0.0	0.001863248227252401	5.323566363578288E-4
22-23	0.0	0.0	0.0	0.003593407295415345	5.323566363578288E-4
24-25	0.0	0.0	0.0	0.007719171227188518	5.323566363578288E-4
26-27	0.0	0.0	0.0	0.01290964843167735	5.323566363578288E-4
28-29	1.330891590894572E-4	0.0	0.0	0.02608547518153361	5.323566363578288E-4
30-31	2.661783181789144E-4	0.0	0.0	0.05004152381763591	5.323566363578288E-4
32-33	2.661783181789144E-4	0.0	0.0	0.08238218947637402	5.323566363578288E-4
34-35	2.661783181789144E-4	0.0	0.0	0.11765081663508017	5.323566363578288E-4
36-37	2.661783181789144E-4	0.0	0.0	0.17807329486169376	5.323566363578288E-4
38-39	2.661783181789144E-4	0.0	0.0	0.26790847724707734	5.323566363578288E-4
40-41	2.661783181789144E-4	0.0	0.0	0.3645312067460233	5.323566363578288E-4
42-43	2.661783181789144E-4	0.0	0.0	0.44185600817699794	5.323566363578288E-4
44-45	2.661783181789144E-4	0.0	0.0	0.5106631034262473	5.323566363578288E-4
46-47	2.661783181789144E-4	0.0	0.0	0.6074189220842827	5.323566363578288E-4
48-49	2.661783181789144E-4	0.0	0.0	0.7080343263559123	5.323566363578288E-4
50-51	2.661783181789144E-4	0.0	0.0	0.8027938076276059	5.323566363578288E-4
52-53	2.661783181789144E-4	0.0	0.0	0.9020783203083409	5.323566363578288E-4
54-55	2.661783181789144E-4	0.0	0.0	1.0000319413981815	5.323566363578288E-4
56-57	2.661783181789144E-4	0.0	0.0	1.1061040011924788	5.323566363578288E-4
58-59	2.661783181789144E-4	0.0	0.0	1.222557015395754	5.323566363578288E-4
60-61	2.661783181789144E-4	0.0	0.0	1.342204169417176	5.323566363578288E-4
62-63	2.661783181789144E-4	0.0	0.0	1.4615851451204191	5.323566363578288E-4
64-65	2.661783181789144E-4	0.0	0.0	1.5645961542556588	5.323566363578288E-4
66-67	2.661783181789144E-4	0.0	0.0	1.6769234045271608	5.323566363578288E-4
68-69	2.661783181789144E-4	0.0	0.0	1.8158684866165542	5.323566363578288E-4
70-71	2.661783181789144E-4	0.0	0.0	1.9698526436830561	5.323566363578288E-4
72-73	2.661783181789144E-4	0.0	0.0	2.1082653691360917	5.323566363578288E-4
74-75	2.661783181789144E-4	0.0	0.0	2.2382934775664913	5.323566363578288E-4
76-77	2.661783181789144E-4	0.0	0.0	2.388684227337578	5.323566363578288E-4
78-79	2.661783181789144E-4	0.0	0.0	2.5651604522901983	5.323566363578288E-4
80-81	2.661783181789144E-4	0.0	0.0	2.7474926002427544	5.323566363578288E-4
82-83	2.661783181789144E-4	0.0	0.0	2.9354144928770682	5.323566363578288E-4
84-85	2.661783181789144E-4	0.0	0.0	3.1098943804433468	5.323566363578288E-4
86-87	2.661783181789144E-4	0.0	0.0	3.2759896509869892	5.323566363578288E-4
88-89	2.661783181789144E-4	0.0	0.0	3.4656417026894655	5.323566363578288E-4
90-91	2.661783181789144E-4	0.0	0.0	3.6652754413236517	5.323566363578288E-4
92-93	2.661783181789144E-4	0.0	0.0	3.8814122356849303	5.323566363578288E-4
94-95	2.661783181789144E-4	0.0	0.0	4.071729733182854	5.323566363578288E-4
96-97	2.661783181789144E-4	0.0	0.0	4.282010604544196	7.985349545367432E-4
98-99	2.661783181789144E-4	0.0	0.0	4.505600391814484	7.985349545367432E-4
100-101	2.661783181789144E-4	0.0	0.0	4.759268329038989	7.985349545367432E-4
102-103	2.661783181789144E-4	0.0	0.0	5.032899640126914	7.985349545367432E-4
104-105	2.661783181789144E-4	0.0	0.0	5.317311173101084	7.985349545367432E-4
106-107	2.661783181789144E-4	0.0	0.0	5.601323438597985	7.985349545367432E-4
108-109	2.661783181789144E-4	0.0	0.0	5.886799684844871	7.985349545367432E-4
110-111	2.661783181789144E-4	0.0	0.0	6.177466408296246	7.985349545367432E-4
112-113	2.661783181789144E-4	0.0	0.0	6.481708225974745	7.985349545367432E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAACG	75	2.3072062E-8	55.532715	1
CGTTAAC	55	8.5215364E-4	43.27224	1
GCGCAGA	245	0.0	36.428165	1
GTATCAA	2735	0.0	35.895393	1
GGTATCA	1905	0.0	33.731907	1
CATGGGG	1480	0.0	30.54558	4
TATCAAC	3290	0.0	29.651346	2
ATCAACG	3275	0.0	29.423899	3
CAACGCA	3340	0.0	29.207464	5
TCAACGC	3395	0.0	29.084715	4
TGATCGC	105	5.907767E-4	28.325476	9
AACGCAG	3530	0.0	28.309425	6
ACCTACT	85	0.007214053	27.992231	8
ATGGGGG	1020	0.0	27.409061	5
ACGCAGA	3685	0.0	26.6344	7
TCGACGG	45	0.009539792	26.437109	114-115
CGCAGAG	3850	0.0	25.492926	8
GTATAAG	215	1.227545E-7	24.906698	1
GTACTAG	120	0.0012863326	24.79139	1
GTGATCG	120	0.0012883306	24.78479	8
>>END_MODULE