FastQCFastQC Report
Sun 1 Jan 2017
ERR1142664_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142664_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415245
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11060.26634878204433526No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7900.19024913003166805No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATCG558.468436E-443.3282553
CATGGGG16150.026.1914624
GGTATCA19900.026.0459441
ATGGGGG9800.025.535815
GTATCAA25000.022.6390151
AACGCAG26750.021.3832576
GTACATG39250.020.6430151
TATCAAC28450.020.5219062
ACATGGG37950.020.4082363
TCAACGC27300.020.295244
ATCAACG26900.020.1540833
TACATGG39550.020.0345272
CAACGCA27400.020.0061575
GTATAAA3902.6739144E-1019.8587841
ACGCAGA29700.019.2593297
TGGGGGG6500.019.250076
ACAGGGG2851.8268565E-618.8135853
CGCAGAG29950.018.700688
GGATTAG1600.00677511718.6176091
CCTAGCC1650.0080908518.053443
Produced by FastQC (version 0.11.5)