##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142664_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415245
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.316437284013052	33.0	33.0	33.0	27.0	33.0
2	31.438240075136367	33.0	33.0	33.0	27.0	33.0
3	31.47744102879023	33.0	33.0	33.0	27.0	33.0
4	31.430562679863694	33.0	33.0	33.0	27.0	33.0
5	31.482943804260135	33.0	33.0	33.0	27.0	33.0
6	34.817445122758855	37.0	37.0	37.0	27.0	37.0
7	34.82229767968308	37.0	37.0	37.0	27.0	37.0
8	34.770073089380965	37.0	37.0	37.0	27.0	37.0
9	34.707018747968064	37.0	37.0	37.0	27.0	37.0
10-11	34.78471143541765	37.0	37.0	37.0	27.0	37.0
12-13	34.75610663584149	37.0	37.0	37.0	27.0	37.0
14-15	34.748219725704104	37.0	37.0	37.0	27.0	37.0
16-17	34.776553600886224	37.0	37.0	37.0	27.0	37.0
18-19	34.7498320268757	37.0	37.0	37.0	27.0	37.0
20-21	34.770118845500846	37.0	37.0	37.0	27.0	37.0
22-23	34.85221495743477	37.0	37.0	37.0	27.0	37.0
24-25	34.90539199749546	37.0	37.0	37.0	27.0	37.0
26-27	34.97115197052342	37.0	37.0	37.0	27.0	37.0
28-29	34.994535755999465	37.0	37.0	37.0	27.0	37.0
30-31	35.01038302688774	37.0	37.0	37.0	27.0	37.0
32-33	35.01974256162025	37.0	37.0	37.0	27.0	37.0
34-35	35.021437946272684	37.0	37.0	37.0	27.0	37.0
36-37	35.01544871100194	37.0	37.0	37.0	27.0	37.0
38-39	34.98148081253236	37.0	37.0	37.0	27.0	37.0
40-41	34.95078447663428	37.0	37.0	37.0	27.0	37.0
42-43	34.94747438259341	37.0	37.0	37.0	27.0	37.0
44-45	34.97434044961408	37.0	37.0	37.0	27.0	37.0
46-47	34.97909908608172	37.0	37.0	37.0	27.0	37.0
48-49	34.950113788245496	37.0	37.0	37.0	27.0	37.0
50-51	34.94621127286301	37.0	37.0	37.0	27.0	37.0
52-53	34.92364628111115	37.0	37.0	37.0	27.0	37.0
54-55	34.905772495755514	37.0	37.0	37.0	27.0	37.0
56-57	34.88955315536611	37.0	37.0	37.0	27.0	37.0
58-59	34.86665823790774	37.0	37.0	37.0	27.0	37.0
60-61	34.856034389336415	37.0	37.0	37.0	27.0	37.0
62-63	34.79477778179147	37.0	37.0	37.0	27.0	37.0
64-65	34.76470637816229	37.0	37.0	37.0	27.0	37.0
66-67	34.704846536382135	37.0	37.0	37.0	27.0	37.0
68-69	34.6622885284591	37.0	37.0	37.0	27.0	37.0
70-71	34.63010150633963	37.0	37.0	37.0	27.0	37.0
72-73	34.57002733326109	37.0	37.0	37.0	27.0	37.0
74-75	34.51896109525701	37.0	37.0	37.0	27.0	37.0
76-77	34.46105913376441	37.0	37.0	37.0	27.0	37.0
78-79	34.41014942985466	37.0	37.0	37.0	27.0	37.0
80-81	34.355562378836595	37.0	37.0	37.0	27.0	37.0
82-83	34.31754024732387	37.0	37.0	37.0	27.0	37.0
84-85	34.27757829714987	37.0	37.0	37.0	27.0	37.0
86-87	34.214070006863416	37.0	37.0	37.0	27.0	37.0
88-89	34.1566846078821	37.0	37.0	37.0	27.0	37.0
90-91	34.08516418018279	37.0	37.0	37.0	27.0	37.0
92-93	34.03076256186107	37.0	37.0	37.0	27.0	37.0
94-95	33.982250237811414	37.0	37.0	37.0	24.5	37.0
96-97	33.8840636250888	37.0	37.0	37.0	22.0	37.0
98-99	33.80298859709328	37.0	37.0	37.0	22.0	37.0
100-101	33.734031716215725	37.0	35.0	37.0	22.0	37.0
102-103	33.688646461727416	37.0	33.0	37.0	22.0	37.0
104-105	33.517492082987154	37.0	33.0	37.0	22.0	37.0
106-107	33.432108755072306	37.0	33.0	37.0	22.0	37.0
108-109	33.32919842502619	37.0	33.0	37.0	22.0	37.0
110-111	33.24269407217426	37.0	33.0	37.0	22.0	37.0
112-113	33.14421124878084	37.0	33.0	37.0	14.0	37.0
114-115	33.022867222964756	37.0	33.0	37.0	14.0	37.0
116-117	32.92960782188828	37.0	33.0	37.0	14.0	37.0
118-119	32.81167623932859	37.0	33.0	37.0	14.0	37.0
120-121	32.6818263916483	37.0	33.0	37.0	14.0	37.0
122-123	32.56425483750557	37.0	33.0	37.0	14.0	37.0
124-125	30.98565304820046	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	747.0
1	41.0
2	25.0
3	16.0
4	9.0
5	6.0
6	7.0
7	16.0
8	15.0
9	11.0
10	13.0
11	17.0
12	17.0
13	10.0
14	33.0
15	166.0
16	252.0
17	473.0
18	926.0
19	1640.0
20	2512.0
21	3025.0
22	3300.0
23	3570.0
24	3747.0
25	4295.0
26	5011.0
27	6009.0
28	7209.0
29	8714.0
30	10445.0
31	12759.0
32	16214.0
33	22161.0
34	32778.0
35	63304.0
36	205752.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.8233894481041	19.742108784872446	12.308290637312096	24.126211129711358
2	17.23034569362103	23.00848839034521	35.956241925162104	23.80492399087166
3	19.69994059664154	27.683896860315176	28.047561323101146	24.568601219942142
4	14.673029689005674	19.286623403911037	33.90941870602981	32.13092820105347
5	15.516937383008273	37.715838415554614	30.74790169675744	16.01932250467967
6	29.42502627795431	35.968538945740555	18.373545651149584	16.232889125155552
7	26.328037776155977	33.363943490427886	21.3919082855251	18.91611044789104
8	25.44786669727764	34.036418999746246	20.525864256455492	19.989850046520623
9	26.609508435994222	18.67735403144347	19.700982548052064	35.012154984510246
10-11	24.942545124129115	27.636218200448038	25.535460110245694	21.88577656517715
12-13	25.69564192883771	26.500552840026824	25.234881486807364	22.568923744328103
14-15	24.432501271714298	26.76907239165659	23.313876003929327	25.48455033269979
16-17	23.89489636351561	28.529044177579742	24.108388125124527	23.46767133378012
18-19	24.00024148756339	27.696450132818164	25.507848345810192	22.79546003380826
20-21	25.70893945795859	26.35625037725599	24.837810104424456	23.09700006036096
22-23	25.63668298335939	26.180988538076644	24.68001376155095	23.50231471701302
24-25	24.529522600175987	26.828829633527953	24.818007235049798	23.823640531246266
26-27	24.25292240242966	26.792310255085532	24.986480418334484	23.968286924150316
28-29	24.05755680487799	26.817237109221303	25.287192621219546	23.83801346468116
30-31	24.588691816080576	26.51563339227413	25.31481023276585	23.58086455887944
32-33	24.069862881421397	26.79316338354577	25.07797412128235	24.058999613750483
34-35	24.18512304925591	26.897758681037498	25.119307689522408	23.79781058018418
36-37	24.25705351097508	26.937490269250592	24.912408168881154	23.893048050893174
38-39	24.135958258048397	26.89509836225844	24.90129978914183	24.06764359055133
40-41	23.972534136231165	26.91908069002552	25.117508281392357	23.99087689235095
42-43	24.063471527603454	26.849402359665554	25.073739548535347	24.013386564195645
44-45	24.014915646948083	27.03400670962759	24.995655636811236	23.955422006613087
46-47	23.935979572159344	26.84822601918972	25.154675004495136	24.0611194041558
48-49	23.985358753973486	27.161852626904075	25.010439068424773	23.842349550697666
50-51	23.682308029318655	27.239240553689513	25.088015101061973	23.99043631592986
52-53	23.735692665665777	27.002785434638838	24.99927588354276	24.262246016152623
54-55	23.659254217324037	26.844242016108982	25.17420824855239	24.322295518014595
56-57	23.911645122632642	26.654541987634754	24.928887188591055	24.50492570114155
58-59	23.882733310242372	26.82212436052314	25.049390121010322	24.245752208224165
60-61	23.898621126633074	26.822556799324143	25.284494463385933	23.994327610656853
62-63	23.526245537643103	26.912809347838156	25.222725616040588	24.338219498478153
64-65	23.636813488543307	26.652787081123158	25.279673648701657	24.430725781631875
66-67	24.008676831483815	26.700329425057305	25.056221956922453	24.234771786536427
68-69	23.990256538391435	26.558175297814117	24.982829442346155	24.46873872144829
70-71	23.762718042993594	26.496380585890016	25.319482551128676	24.42141881998771
72-73	24.158980431636266	26.62593897606192	25.20445324825664	24.010627344045172
74-75	24.137194260757457	26.554619617045834	25.192841156829655	24.115344965367054
76-77	24.05183693768076	26.33467078040278	25.271231368686358	24.342260913230103
78-79	23.997387848910474	26.47011959885857	25.348608703629488	24.18388384860146
80-81	23.82376159701417	26.600406061469307	25.36665033086043	24.209182010656097
82-83	23.96419100864567	26.381189295926287	25.49091317273119	24.163706522696852
84-85	23.85674751592787	26.64670478011149	25.174183753148462	24.32236395081217
86-87	23.665423741085327	26.74003547768164	25.29534566605124	24.299195115181792
88-89	23.871774235391076	26.247500775257404	25.385245614818334	24.495479374533186
90-91	24.102057267531666	26.3076343836886	25.33475247142416	24.255555877355576
92-93	23.9559137436515	26.700324227531226	25.199851823070958	24.143910205746323
94-95	23.442235671126042	26.695551244184472	25.409258508523003	24.452954576166483
96-97	23.530383987588475	26.6758514525755	25.30790547069816	24.485859089137865
98-99	23.40811362550493	26.937193241541067	25.139353400222962	24.515339732731036
100-101	23.547857015312893	27.109926596560996	25.03619498964823	24.30602139847788
102-103	23.619672241908297	27.08040002750823	25.26268545430196	24.037242276281514
104-105	23.619986485834257	27.20353298904387	25.172546937593516	24.003933587528355
106-107	23.538666460754413	27.209012200301146	25.258315316010965	23.99400602293348
108-109	23.520156186463275	27.199660212391386	25.046787160951457	24.233396440193882
110-111	23.296042003718277	27.757248042235354	24.930659360413284	24.016050593633086
112-113	23.342606756971957	27.698542150243938	24.832064973482673	24.12678611930143
114-115	23.284575719480443	27.789788891275023	25.001749386508248	23.923886002736282
116-117	23.28708948480275	27.75390985526025	25.053235320021184	23.905765339915813
118-119	23.295673207424255	27.49120828080419	25.13653554991223	24.07658296185932
120-121	23.397676037560792	27.5395943501966	25.281386221516016	23.78134339072659
122-123	23.21190514968904	27.81000983248581	25.145104568184973	23.83298044964018
124-125	23.277166528703233	27.77372870516289	24.92826303633788	24.020841729796
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1433.0
1	905.0
2	301.0
3	206.5
4	156.5
5	121.0
6	105.5
7	99.5
8	108.0
9	108.5
10	108.5
11	101.5
12	103.5
13	108.5
14	109.5
15	126.5
16	150.0
17	177.0
18	223.5
19	257.0
20	304.0
21	383.5
22	465.5
23	589.0
24	740.0
25	911.5
26	1166.5
27	1503.0
28	1839.0
29	2358.0
30	3033.0
31	3700.5
32	4528.0
33	5247.5
34	5958.0
35	6742.5
36	7397.0
37	8226.5
38	8845.0
39	9242.0
40	9672.0
41	10043.5
42	10607.5
43	11524.0
44	12431.5
45	13200.5
46	14416.0
47	15552.5
48	16662.5
49	17671.5
50	17880.5
51	17615.5
52	17172.5
53	16829.5
54	16489.5
55	15943.5
56	15240.5
57	14054.0
58	12636.0
59	11305.5
60	9782.5
61	8214.0
62	6747.0
63	5274.0
64	4070.0
65	3258.0
66	2599.5
67	2031.0
68	1568.0
69	1247.0
70	1113.0
71	995.5
72	833.5
73	785.5
74	615.5
75	472.0
76	390.0
77	262.5
78	182.5
79	130.0
80	91.0
81	60.5
82	38.0
83	22.5
84	16.0
85	10.0
86	6.5
87	2.5
88	1.5
89	1.5
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.306325181519344
2	0.2769449361220484
3	0.27140603739960745
4	0.2856145167310865
5	0.2938024539729557
6	0.3371503570181459
7	0.3465424026779371
8	0.3491914411973654
9	0.3426892557405869
10-11	0.3472648677286903
12-13	0.3546099290780142
14-15	0.34521788341822296
16-17	0.28356753242061916
18-19	0.2757408277041265
20-21	0.25767920143529727
22-23	0.25226071355464846
24-25	0.2435911329456104
26-27	0.24828715577550603
28-29	0.23504196317836457
30-31	0.23781141253958504
32-33	0.2420257920023119
34-35	0.23600524991270214
36-37	0.2325133355007285
38-39	0.23732976917241627
40-41	0.21974978627075584
42-43	0.22853977772158604
44-45	0.2203518404797168
46-47	0.21878649953641824
48-49	0.22540909583498897
50-51	0.23347662223506602
52-53	0.22805813435441724
54-55	0.2268540259364953
56-57	0.22601115004394998
58-59	0.22673361509470313
60-61	0.23058676203205336
62-63	0.22781731267083288
64-65	0.23299497886789727
66-67	0.2500933184023889
68-69	0.24527688473070117
70-71	0.24575852809786994
72-73	0.24816674493371382
74-75	0.25141783766210307
76-77	0.24636058230683092
78-79	0.2473238690411685
80-81	0.24611976062324653
82-83	0.23877469927392264
84-85	0.2301051186648846
86-87	0.21673951522595097
88-89	0.2086719888258739
90-91	0.21890691037821045
92-93	0.2108393839781334
94-95	0.2015677491601344
96-97	0.19061036255704464
98-99	0.20012281905862805
100-101	0.19819624558995294
102-103	0.1983166564317451
104-105	0.20831075630049728
106-107	0.19964117569145926
108-109	0.20915363219304264
110-111	0.1939818661272261
112-113	0.19241652518392757
114-115	0.1960288504376934
116-117	0.19542679622873244
118-119	0.19085118424062902
120-121	0.1983166564317451
122-123	0.19325940107647294
124-125	0.21288636828860072
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	415245.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.58181242882732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.43890624431218	71.26567354389809
2	8.176324784704246	13.177261403164191
3	1.735752240841027	4.196101844831051
4	0.6259015564112965	2.017451272705847
5	0.3122793238294467	1.2582016949112746
6	0.18269363719903078	0.8833070642827517
7	0.11301940187428883	0.6375115769856539
8	0.07000303314125583	0.45127770288301283
9	0.057877530959329404	0.4197488709247496
>10	0.2654867146547484	3.7875163973823525
>50	0.017336439624851745	0.9195558418166737
>100	0.0037392320716717843	0.4670368510123322
>500	3.399301883337986E-4	0.21639830633415985
>1k	3.399301883337986E-4	0.30295762886782374
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1106	0.26634878204433526	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	790	0.19024913003166805	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.2041084179219497E-4	0.0
12-13	0.0	0.0	0.0	4.816433671687799E-4	0.0
14-15	0.0	0.0	0.0	4.816433671687799E-4	0.0
16-17	0.0	0.0	0.0	4.816433671687799E-4	0.0
18-19	0.0	0.0	0.0	0.0012041084179219496	0.0
20-21	0.0	0.0	0.0	0.002046984310467314	0.0
22-23	0.0	0.0	0.0	0.0034919144119736545	0.0
24-25	0.0	0.0	0.0	0.0071042396657395035	0.0
26-27	0.0	0.0	0.0	0.012402316704596083	0.0
28-29	0.0	0.0	0.0	0.02504545509277655	0.0
30-31	0.0	0.0	0.0	0.04491324398848873	0.0
32-33	0.0	0.0	0.0	0.07778540379775795	0.0
34-35	0.0	0.0	0.0	0.11896591169068864	0.0
36-37	0.0	0.0	0.0	0.18362653373309734	0.0
38-39	0.0	0.0	0.0	0.2571975580681285	0.0
40-41	0.0	0.0	0.0	0.3530445881347156	0.0
42-43	0.0	0.0	0.0	0.43239533287577214	0.0
44-45	0.0	0.0	0.0	0.511384845091452	0.0
46-47	0.0	0.0	0.0	0.607111464316247	0.0
48-49	0.0	0.0	0.0	0.7072932846873532	0.0
50-51	0.0	0.0	0.0	0.8061505857987453	0.0
52-53	0.0	0.0	0.0	0.9010343291309949	0.0
54-55	0.0	0.0	0.0	0.9929078014184398	0.0
56-57	0.0	0.0	0.0	1.0983876988284025	0.0
58-59	0.0	0.0	0.0	1.2024226661368589	0.0
60-61	0.0	0.0	0.0	1.3066984551288998	0.0
62-63	0.0	0.0	0.0	1.4085660272850968	2.4082168358438994E-4
64-65	0.0	2.4082168358438994E-4	0.0	1.5156112656383582	4.816433671687799E-4
66-67	0.0	2.4082168358438994E-4	0.0	1.6342159448036702	4.816433671687799E-4
68-69	0.0	2.4082168358438994E-4	0.0	1.773049645390071	4.816433671687799E-4
70-71	0.0	2.4082168358438994E-4	0.0	1.9133282760779782	4.816433671687799E-4
72-73	0.0	2.4082168358438994E-4	0.0	2.0433719852135486	4.816433671687799E-4
74-75	0.0	2.4082168358438994E-4	0.0	2.165348167949042	4.816433671687799E-4
76-77	0.0	2.4082168358438994E-4	0.0	2.3087574805235462	4.816433671687799E-4
78-79	0.0	2.4082168358438994E-4	0.0	2.461558838757842	4.816433671687799E-4
80-81	0.0	2.4082168358438994E-4	0.0	2.619778684872786	4.816433671687799E-4
82-83	0.0	2.4082168358438994E-4	0.0	2.7961805680983516	4.816433671687799E-4
84-85	0.0	2.4082168358438994E-4	0.0	2.9629495839805413	4.816433671687799E-4
86-87	0.0	2.4082168358438994E-4	0.0	3.1332105142747055	4.816433671687799E-4
88-89	0.0	2.4082168358438994E-4	0.0	3.3276740237691	4.816433671687799E-4
90-91	0.0	2.4082168358438994E-4	0.0	3.5223783549470795	4.816433671687799E-4
92-93	0.0	2.4082168358438994E-4	0.0	3.724788979999759	4.816433671687799E-4
94-95	0.0	2.4082168358438994E-4	0.0	3.934665077243555	4.816433671687799E-4
96-97	0.0	2.4082168358438994E-4	0.0	4.150441305735169	4.816433671687799E-4
98-99	0.0	2.4082168358438994E-4	0.0	4.386687377331455	4.816433671687799E-4
100-101	0.0	2.4082168358438994E-4	0.0	4.640513431829402	4.816433671687799E-4
102-103	0.0	2.4082168358438994E-4	0.0	4.895302773061687	4.816433671687799E-4
104-105	0.0	2.4082168358438994E-4	0.0	5.152741152813399	4.816433671687799E-4
106-107	0.0	2.4082168358438994E-4	0.0	5.42595335283989	4.816433671687799E-4
108-109	0.0	2.4082168358438994E-4	0.0	5.712410745463521	4.816433671687799E-4
110-111	0.0	2.4082168358438994E-4	0.0	6.009584703006659	4.816433671687799E-4
112-113	0.0	2.4082168358438994E-4	0.0	6.316511938734964	4.816433671687799E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATCG	55	8.468436E-4	43.328255	3
CATGGGG	1615	0.0	26.191462	4
GGTATCA	1990	0.0	26.045944	1
ATGGGGG	980	0.0	25.53581	5
GTATCAA	2500	0.0	22.639015	1
AACGCAG	2675	0.0	21.383257	6
GTACATG	3925	0.0	20.643015	1
TATCAAC	2845	0.0	20.521906	2
ACATGGG	3795	0.0	20.408236	3
TCAACGC	2730	0.0	20.29524	4
ATCAACG	2690	0.0	20.154083	3
TACATGG	3955	0.0	20.034527	2
CAACGCA	2740	0.0	20.006157	5
GTATAAA	390	2.6739144E-10	19.858784	1
ACGCAGA	2970	0.0	19.259329	7
TGGGGGG	650	0.0	19.25007	6
ACAGGGG	285	1.8268565E-6	18.813585	3
CGCAGAG	2995	0.0	18.70068	8
GGATTAG	160	0.006775117	18.617609	1
CCTAGCC	165	0.00809085	18.05344	3
>>END_MODULE