FastQCFastQC Report
Sun 1 Jan 2017
ERR1142664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415245
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22080.531734277354333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19760.47586364676275456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13890.3345013184987176No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.12980288745198618No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4410.10620236246071596No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15300.044.710311
GTATCAA25300.043.966551
TATCAAC32050.035.2593732
ATCAACG33200.034.2171863
TCAACGC33800.033.7857484
CAACGCA34350.033.0716325
AACGCAG36000.032.2089466
ACGCAGA39650.029.0939667
CGCAGAG40000.028.8393948
GCAGAGT42650.027.0474999
GAGTACT19850.023.66524312-13
CATGGGT7200.023.1299424
CATGGGG16500.022.7093964
AGAGTAC41250.022.70392810-11
ATGGGGG9400.022.1456895
CAGAGTA42550.021.94039710-11
GTACATG32550.021.3814031
AGTACTT21300.021.21672612-13
ACATGGG32700.020.9169863
TACATGG33400.020.6566812
Produced by FastQC (version 0.11.5)