##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142664_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415245
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	19.830121976182735	14.0	14.0	27.0	14.0	33.0
2	30.149196257631036	33.0	27.0	33.0	27.0	33.0
3	30.836491709713542	33.0	33.0	33.0	27.0	33.0
4	31.212700935592242	33.0	33.0	33.0	27.0	33.0
5	31.975503618345797	33.0	33.0	33.0	33.0	33.0
6	34.32094546592975	37.0	33.0	37.0	27.0	37.0
7	34.94729858276439	37.0	37.0	37.0	33.0	37.0
8	34.83471203747185	37.0	37.0	37.0	27.0	37.0
9	35.7064094691086	37.0	37.0	37.0	33.0	37.0
10-11	35.804060253585234	37.0	37.0	37.0	33.0	37.0
12-13	35.87091837349035	37.0	37.0	37.0	33.0	37.0
14-15	35.867542053486495	37.0	37.0	37.0	33.0	37.0
16-17	35.90728365182001	37.0	37.0	37.0	33.0	37.0
18-19	35.95512047104721	37.0	37.0	37.0	33.0	37.0
20-21	36.00541607966382	37.0	37.0	37.0	33.0	37.0
22-23	36.029183975725175	37.0	37.0	37.0	37.0	37.0
24-25	36.024372358487156	37.0	37.0	37.0	37.0	37.0
26-27	35.92021818444533	37.0	37.0	37.0	33.0	37.0
28-29	35.872399426844396	37.0	37.0	37.0	33.0	37.0
30-31	35.88908957362521	37.0	37.0	37.0	33.0	37.0
32-33	35.83035196089056	37.0	37.0	37.0	33.0	37.0
34-35	35.76365398740502	37.0	37.0	37.0	33.0	37.0
36-37	35.6912653975364	37.0	37.0	37.0	33.0	37.0
38-39	35.632280942576074	37.0	37.0	37.0	33.0	37.0
40-41	35.55063757540729	37.0	37.0	37.0	33.0	37.0
42-43	35.47602499729075	37.0	37.0	37.0	33.0	37.0
44-45	35.36362508880299	37.0	37.0	37.0	33.0	37.0
46-47	35.21800864549844	37.0	37.0	37.0	33.0	37.0
48-49	35.079687895098076	37.0	37.0	37.0	33.0	37.0
50-51	34.97455237269564	37.0	37.0	37.0	30.0	37.0
52-53	34.89743524906983	37.0	37.0	37.0	27.0	37.0
54-55	34.81759322809425	37.0	37.0	37.0	27.0	37.0
56-57	34.78113162109116	37.0	37.0	37.0	27.0	37.0
58-59	34.719055015713614	37.0	37.0	37.0	27.0	37.0
60-61	34.68568796734458	37.0	37.0	37.0	27.0	37.0
62-63	34.66300497296777	37.0	37.0	37.0	27.0	37.0
64-65	34.63339955929632	37.0	37.0	37.0	27.0	37.0
66-67	34.60103914556467	37.0	37.0	37.0	27.0	37.0
68-69	34.57306891112476	37.0	37.0	37.0	27.0	37.0
70-71	34.51345470746186	37.0	37.0	37.0	27.0	37.0
72-73	34.47628628881745	37.0	37.0	37.0	27.0	37.0
74-75	34.372165829811316	37.0	37.0	37.0	27.0	37.0
76-77	34.30320052017484	37.0	37.0	37.0	27.0	37.0
78-79	34.30403496730845	37.0	37.0	37.0	27.0	37.0
80-81	34.262269262724416	37.0	37.0	37.0	27.0	37.0
82-83	34.217300629748706	37.0	37.0	37.0	27.0	37.0
84-85	34.0988982407976	37.0	37.0	37.0	27.0	37.0
86-87	33.96120603499139	37.0	37.0	37.0	27.0	37.0
88-89	33.90100422642055	37.0	37.0	37.0	27.0	37.0
90-91	33.86781418198895	37.0	37.0	37.0	24.5	37.0
92-93	33.74827872701658	37.0	35.0	37.0	22.0	37.0
94-95	33.75978639116666	37.0	33.0	37.0	22.0	37.0
96-97	33.75042204000049	37.0	33.0	37.0	22.0	37.0
98-99	33.69369528832376	37.0	33.0	37.0	22.0	37.0
100-101	33.61035051596046	37.0	33.0	37.0	22.0	37.0
102-103	33.508126527712555	37.0	33.0	37.0	22.0	37.0
104-105	33.38649592409301	37.0	33.0	37.0	14.0	37.0
106-107	33.27836698816361	37.0	33.0	37.0	14.0	37.0
108-109	33.171934640995076	37.0	33.0	37.0	14.0	37.0
110-111	33.106488940264185	37.0	33.0	37.0	14.0	37.0
112-113	33.019779888981205	37.0	33.0	37.0	14.0	37.0
114-115	32.92326819106792	37.0	33.0	37.0	14.0	37.0
116-117	32.83568134474828	37.0	33.0	37.0	14.0	37.0
118-119	32.71417596840419	37.0	33.0	37.0	14.0	37.0
120-121	32.6214523955737	37.0	33.0	37.0	14.0	37.0
122-123	32.50830473575841	37.0	33.0	37.0	14.0	37.0
124-125	31.221569194090236	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	170.0
16	64.0
17	19.0
18	92.0
19	356.0
20	1247.0
21	2753.0
22	4186.0
23	5548.0
24	4776.0
25	3780.0
26	3585.0
27	4219.0
28	5373.0
29	6981.0
30	9480.0
31	12722.0
32	18106.0
33	26622.0
34	43714.0
35	87152.0
36	174293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.59124133312133	15.977893737683274	16.013548999484446	23.417315929710952
2	17.917855723729364	21.869739551349205	36.410552806174664	23.801851918746763
3	20.39013112740671	25.443774157425135	30.063215691940904	24.102879023227253
4	14.606733455351122	17.59368076293228	34.60577015701763	33.19381562469897
5	15.809704557004098	34.82263063965685	32.500897142870905	16.866767660468152
6	29.952533131681424	34.34279700508384	18.750255877968485	16.954413985266246
7	25.998627316403567	31.860949559898373	22.806776722176064	19.333646401521992
8	26.102421462028442	31.45275680622283	21.57280641548965	20.872015316259077
9	26.861250587002854	15.931317655841731	20.954857975412107	36.25257378174331
10-11	25.461354140326797	25.181398933159944	26.887500150513553	22.46974677599971
12-13	26.726270033353806	24.098785054606317	26.814410769545688	22.36053414249419
14-15	24.662066972510203	24.320341003503955	25.872436754205346	25.14515526978049
16-17	23.346217293405097	26.349986152753196	26.606822478295943	23.69697407554576
18-19	23.314790063697334	24.870738961336077	29.262363183181012	22.552107791785573
20-21	24.76315016303746	23.761926780046334	29.05016833557299	22.424754721343216
22-23	24.32106346855471	23.76596948789269	29.08343267227781	22.82953437127479
24-25	23.59004924803429	24.39463449288974	29.03298052956688	22.982335729509085
26-27	23.208226468711242	24.499632746932534	29.202639405652086	23.08950137870414
28-29	23.185950462979687	24.38584450143891	29.445026430179773	22.98317860540163
30-31	23.62557044636299	24.090476706522658	29.49018049585185	22.793772351262508
32-33	23.31238184686149	24.118893665185613	29.243097448494265	23.32562703945863
34-35	23.29085635089688	24.353844542191407	29.262759651241616	23.092539455670096
36-37	23.43272672977142	24.414579196624565	28.93791595371783	23.214778119886184
38-39	23.463319184803876	24.34955898732653	28.976790391041273	23.210331436828323
40-41	23.250053585147402	24.426759338867658	29.062777709542008	23.260409366442932
42-43	23.44538513315809	24.397125032661872	28.762437099553388	23.395052734626642
44-45	23.35054413921487	24.618837644463603	28.49644665894428	23.534171557377245
46-47	23.368067931144097	24.498728458448525	28.35296837522035	23.78023523518702
48-49	23.46578525930475	24.70011679851654	28.228034052186057	23.606063889992654
50-51	23.279148454526847	25.113005575021973	27.942660357138553	23.665185613312623
52-53	23.448205276403087	24.81053354043998	27.758672590880085	23.982588592276848
54-55	23.236486543437763	24.877451423080675	27.86906959410563	24.016992439375933
56-57	23.403051210731014	24.73033991980638	27.58082577755301	24.285783091909597
58-59	23.38401073111759	24.769952870742532	27.76471976514843	24.08131663299144
60-61	23.374634252068056	24.741056484725885	28.06559982660839	23.818709436597672
62-63	23.16319281388096	24.897470168213946	27.96589964960445	23.973437368300644
64-65	23.064215101927775	24.61185565148286	28.150730291755472	24.173198954833893
66-67	23.31719828053318	24.66856915796698	28.010090428542185	24.00414213295765
68-69	23.188238268973738	24.60788209370372	28.05982010620236	24.14405953112018
70-71	23.00094282937919	24.582439772709485	28.430339599195165	23.986277798716163
72-73	23.251722780193358	24.65041680263074	28.403868082539326	23.693992334636583
74-75	23.187756031774153	24.695955677201407	28.436083501761416	23.680204789263026
76-77	23.151260656126947	24.608075093378588	28.39396061406289	23.84670363643157
78-79	23.12430988788425	24.816823021839152	28.409439830652357	23.649427259624243
80-81	22.900215535406808	24.857734590422524	28.474033401967514	23.768016472203158
82-83	22.9166190039844	24.848011231936848	28.493333445716925	23.742036318361833
84-85	23.008060102372703	24.893251261575998	28.258940734257727	23.83974790179357
86-87	22.935991235775024	24.988055365192128	28.137457409534473	23.938495989498374
88-89	23.04984700066994	24.67664934484902	28.060620326277864	24.212883328203176
90-91	23.154102770533136	24.6251801786601	28.12679274753776	24.093924303269006
92-93	23.05728351347244	24.938794511164122	27.904758229273952	24.099163746089484
94-95	22.73910054101438	24.89051472828266	28.044003626831326	24.32638110387163
96-97	23.001240231670458	24.822935857144575	27.787691603752002	24.38813230743296
98-99	22.784019073077342	25.023540319570376	27.5362737660899	24.656166841262387
100-101	22.93549591205192	25.153584028705943	27.398282941396047	24.512637117846094
102-103	23.057648033445396	25.133656356262822	27.459758599763028	24.348937010528747
104-105	23.039880931594674	25.232796313310786	27.362974398125345	24.3643483569692
106-107	23.07350508040258	25.245952175096274	27.240663450724067	24.43987929377708
108-109	23.008291135048562	25.249847965751655	27.132062066100275	24.60979883309951
110-111	22.886163442491675	25.672380470711488	26.94856372185997	24.49289236493687
112-113	22.795351917635017	25.705581311337227	26.91901980853754	24.580046962490215
114-115	22.824792494770975	25.67224710130879	26.94852618003735	24.55443422388288
116-117	22.783285931370305	25.713738702398352	26.899189149485963	24.603786216745377
118-119	22.683289824777724	25.392901522959992	27.182812996695922	24.740995655566365
120-121	22.77751688762056	25.500848896434636	27.24993678430806	24.471697431636745
122-123	22.62074197160712	25.80548832616889	27.054871220604703	24.518898481619285
124-125	22.585061536182987	25.753804695317328	27.007526908910073	24.653606859589612
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	182.0
1	169.5
2	287.0
3	384.5
4	284.0
5	241.0
6	365.0
7	753.0
8	1206.5
9	1362.0
10	1200.0
11	967.5
12	879.0
13	853.5
14	832.5
15	816.5
16	822.0
17	831.5
18	806.0
19	773.0
20	765.5
21	783.0
22	829.5
23	880.0
24	889.0
25	1005.0
26	1226.5
27	1442.5
28	1728.5
29	2182.5
30	2696.0
31	3307.0
32	4093.5
33	4785.5
34	5393.5
35	6190.0
36	6928.0
37	7639.5
38	8316.0
39	8784.0
40	9111.5
41	9478.0
42	10097.5
43	11073.0
44	12099.0
45	12886.0
46	14029.5
47	15148.0
48	16311.0
49	17275.0
50	17656.5
51	17450.0
52	16765.0
53	16507.5
54	16416.5
55	16025.5
56	15059.5
57	13822.5
58	12662.0
59	11272.5
60	9791.5
61	8187.0
62	6659.5
63	5262.0
64	4056.0
65	3258.5
66	2532.0
67	1899.0
68	1498.0
69	1252.5
70	1081.0
71	941.0
72	843.5
73	784.5
74	597.5
75	464.5
76	387.5
77	264.0
78	181.0
79	116.0
80	76.5
81	60.5
82	42.5
83	25.5
84	17.0
85	9.0
86	5.5
87	5.0
88	3.0
89	2.5
90	2.0
91	1.0
92	1.0
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038290647689918
2	0.0
3	0.0
4	0.0012041084179219496
5	0.009151223976206819
6	0.0019265734686751193
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.224650507531698E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.2041084179219496E-4
36-37	0.0022878059940517046
38-39	0.0018061626268829245
40-41	0.004334790304519019
42-43	0.0013245192597141446
44-45	4.8164336716877983E-4
46-47	2.4082168358438992E-4
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.612325253765849E-4
56-57	0.0
58-59	9.632867343375597E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0013245192597141446
72-73	1.2041084179219496E-4
74-75	0.09139182892027599
76-77	0.09668990595913256
78-79	0.0010836975761297548
80-81	0.0
82-83	1.2041084179219496E-4
84-85	0.11703933822201351
86-87	0.2006044624257968
88-89	0.24744427988296064
90-91	0.09127141807848378
92-93	0.15846066779852858
94-95	0.0015653409432985347
96-97	0.0
98-99	0.0
100-101	0.0
102-103	2.4082168358438992E-4
104-105	0.005177666197064383
106-107	0.0052980770388565785
108-109	0.010234921552336572
110-111	0.005418487880648773
112-113	0.0048164336716877984
114-115	0.003491914411973654
116-117	4.8164336716877983E-4
118-119	2.4082168358438992E-4
120-121	0.0
122-123	0.0
124-125	3.612325253765849E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	415245.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.1373368073577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.99202424201171	68.75462434559252
2	8.480350084155479	13.252639415399218
3	1.787961277195595	4.191195975442369
4	0.6585458765186575	2.0582808382653974
5	0.3151331667668732	1.2311833195416195
6	0.17680598880760837	0.8289089458210805
7	0.12874742201553924	0.7041986473975046
8	0.08059951599750075	0.5038265222405384
9	0.058203369886354085	0.4093070684519942
>10	0.2892508420694145	4.077668814523271
>50	0.02342253820925379	1.2259479435692024
>100	0.007577880002043677	1.1172140307656948
>500	3.4444909100198533E-4	0.14506826369132966
>1k	0.001033347273005956	1.4999358692982936
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2208	0.531734277354333	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1976	0.47586364676275456	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1389	0.3345013184987176	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	539	0.12980288745198618	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	441	0.10620236246071596	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.2041084179219497E-4	0.0
12-13	0.0	0.0	0.0	4.816433671687799E-4	0.0
14-15	0.0	0.0	0.0	4.816433671687799E-4	0.0
16-17	0.0	0.0	0.0	4.816433671687799E-4	0.0
18-19	0.0	0.0	0.0	0.0010836975761297548	0.0
20-21	0.0	0.0	0.0	0.0018061626268829245	2.4082168358438994E-4
22-23	0.0	0.0	0.0	0.003371503570181459	2.4082168358438994E-4
24-25	0.0	0.0	0.0	0.0071042396657395035	2.4082168358438994E-4
26-27	0.0	0.0	0.0	0.012522727546388277	2.4082168358438994E-4
28-29	0.0	0.0	0.0	0.025406687618153137	2.4082168358438994E-4
30-31	0.0	0.0	0.0	0.0453948873556575	2.4082168358438994E-4
32-33	0.0	0.0	0.0	0.07826704716492673	2.4082168358438994E-4
34-35	0.0	0.0	0.0	0.1195679658996496	2.4082168358438994E-4
36-37	0.0	0.0	0.0	0.1839877662584739	2.4082168358438994E-4
38-39	0.0	0.0	0.0	0.2576792014352972	2.4082168358438994E-4
40-41	0.0	0.0	0.0	0.35328540981830003	2.4082168358438994E-4
42-43	0.0	0.0	0.0	0.4333586196101097	2.4082168358438994E-4
44-45	0.0	0.0	0.0	0.5129501860347505	2.4082168358438994E-4
46-47	0.0	0.0	0.0	0.6086768052595455	2.4082168358438994E-4
48-49	0.0	0.0	0.0	0.7086178039470674	2.4082168358438994E-4
50-51	0.0	0.0	0.0	0.8079567484256283	2.4082168358438994E-4
52-53	0.0	0.0	0.0	0.903562956808631	2.4082168358438994E-4
54-55	0.0	0.0	0.0	0.9962793049886212	2.4082168358438994E-4
56-57	0.0	0.0	0.0	1.102361256607545	2.4082168358438994E-4
58-59	0.0	0.0	0.0	1.2069982781249622	2.4082168358438994E-4
60-61	1.2041084179219497E-4	0.0	0.0	1.311153656275211	2.4082168358438994E-4
62-63	2.4082168358438994E-4	0.0	0.0	1.4129008175896158	2.4082168358438994E-4
64-65	2.4082168358438994E-4	0.0	0.0	1.5193440017339161	2.4082168358438994E-4
66-67	2.4082168358438994E-4	0.0	0.0	1.6379486808992283	2.4082168358438994E-4
68-69	2.4082168358438994E-4	0.0	0.0	1.7765415598020446	2.4082168358438994E-4
70-71	2.4082168358438994E-4	0.0	0.0	1.9160977254391987	2.4082168358438994E-4
72-73	2.4082168358438994E-4	0.0	0.0	2.045298558682224	2.4082168358438994E-4
74-75	2.4082168358438994E-4	0.0	0.0	2.1682380281520546	2.4082168358438994E-4
76-77	2.4082168358438994E-4	0.0	0.0	2.3122493949355203	2.4082168358438994E-4
78-79	2.4082168358438994E-4	0.0	0.0	2.4649303423280235	2.4082168358438994E-4
80-81	2.4082168358438994E-4	0.0	0.0	2.6221869017086297	2.4082168358438994E-4
82-83	2.4082168358438994E-4	0.0	0.0	2.7990704283013645	2.4082168358438994E-4
84-85	2.4082168358438994E-4	0.0	0.0	2.964996568291009	2.4082168358438994E-4
86-87	2.4082168358438994E-4	0.0	0.0	3.134775855218004	2.4082168358438994E-4
88-89	2.4082168358438994E-4	0.0	0.0	3.3288781321870218	3.612325253765849E-4
90-91	2.4082168358438994E-4	0.0	0.0	3.5231008199978326	4.816433671687799E-4
92-93	2.4082168358438994E-4	0.0	0.0	3.7258726775758886	4.816433671687799E-4
94-95	2.4082168358438994E-4	0.0	0.0	3.9337017905092173	4.816433671687799E-4
96-97	2.4082168358438994E-4	0.0	0.0	4.150682127418753	4.816433671687799E-4
98-99	2.4082168358438994E-4	0.0	0.0	4.387409842382208	4.816433671687799E-4
100-101	2.4082168358438994E-4	0.0	0.0	4.642560416139869	4.816433671687799E-4
102-103	2.4082168358438994E-4	0.0	0.0	4.897590579055738	4.816433671687799E-4
104-105	2.4082168358438994E-4	0.0	0.0	5.155751423858204	4.816433671687799E-4
106-107	2.4082168358438994E-4	0.0	0.0	5.430528964827993	4.816433671687799E-4
108-109	2.4082168358438994E-4	0.0	0.0	5.717708822502378	9.632867343375598E-4
110-111	2.4082168358438994E-4	0.0	0.0	6.015123601729099	9.632867343375598E-4
112-113	2.4082168358438994E-4	0.0	0.0	6.320485496514106	9.632867343375598E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1530	0.0	44.71031	1
GTATCAA	2530	0.0	43.96655	1
TATCAAC	3205	0.0	35.259373	2
ATCAACG	3320	0.0	34.217186	3
TCAACGC	3380	0.0	33.785748	4
CAACGCA	3435	0.0	33.071632	5
AACGCAG	3600	0.0	32.208946	6
ACGCAGA	3965	0.0	29.093966	7
CGCAGAG	4000	0.0	28.839394	8
GCAGAGT	4265	0.0	27.047499	9
GAGTACT	1985	0.0	23.665243	12-13
CATGGGT	720	0.0	23.129942	4
CATGGGG	1650	0.0	22.709396	4
AGAGTAC	4125	0.0	22.703928	10-11
ATGGGGG	940	0.0	22.145689	5
CAGAGTA	4255	0.0	21.940397	10-11
GTACATG	3255	0.0	21.381403	1
AGTACTT	2130	0.0	21.216726	12-13
ACATGGG	3270	0.0	20.916986	3
TACATGG	3340	0.0	20.656681	2
>>END_MODULE