FastQCFastQC Report
Sun 1 Jan 2017
ERR1142663_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142663_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171720
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3830.22303750291171676No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA2810.16363848124854413No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC2370.13801537386443047No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2190.1275331935709294No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT2180.12695085022129046No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA2160.12578616352201258No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA2080.121127416724901No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA2070.12054507337526206No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT2020.11763335662706732No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2000.11646866992778944No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1980.11530398322851154No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTG1880.10948054973212205No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1870.10889820638248311No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC1830.10656883298392733No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1730.10074539948753784No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC1720.1001630561378989No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCCG300.004211906359.565889
GTACAAA902.3690923E-433.0921551
TATTCTC800.00532075529.7829365
CGCACAA400.005358623329.748175110-111
TCTGTAC900.00946908926.4659923
AGTACAA900.00946908926.4659922
GTTAATG450.00952265626.44282348-49
GTATCGG1159.948683E-425.8982071
TATCGGC1159.965667E-425.8906442
TCGGCTT1250.001617419823.826354
TACTGAT1250.001617419823.826357
GTATCAA14550.023.7444731
TACCAGT1300.00203196122.9099521
CCTCTAA1300.00203196122.9099521
AACGCAG15350.022.5069776
ATGGGGG7500.022.21197118-19
CGCAGAG15500.021.9048718
ACGCAGA15550.021.8344367
CAACGCA15650.021.6949215
ACTGATT1653.175999E-421.660328
Produced by FastQC (version 0.11.5)