FastQCFastQC Report
Sun 1 Jan 2017
ERR1142663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171720
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13470.7844164919636618No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12300.716282320055905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.40822268809690193No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.23817843000232936No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC2680.15606801770323783No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2630.1531563009550431No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA2430.14150943396226415No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT2360.13743303051479153No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC2180.12695085022129046No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA2130.12403913347309574No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2010.11705101327742838No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA2010.11705101327742838No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC1880.10948054973212205No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT1840.10715117633356627No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGA1810.10540414628464943No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1760.10249242953645471No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTA150.004184613559.67660592-93
GAGTAGT709.11994E-750.9800261
AGTAGTG709.1382935E-750.9651835
GGTTTAA600.001307922939.6511341
GGTATCA13800.038.358161
TATTCTC1401.886292E-938.223895
TAATAAT801.1980343E-437.162113
AGTAGTA801.1980343E-437.162112
GTAGTGT801.1980343E-437.162116
TAAGTAC350.002748040534.09593680-81
GTCTACA700.00278776233.9866871
GTATCAA18950.032.6415631
TAGTAGT1004.4408257E-429.7296874
TATCAAC21850.028.0289062
GAATAGC854.435842E-727.98088540-41
CGTAACC854.435842E-727.98088548-49
CAACGCA22650.027.563955
ATCAACG22600.026.8356483
AACGCAG24100.026.3989776
TCAACGC24850.025.3629534
Produced by FastQC (version 0.11.5)