##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142662_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	581404
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.287897916079007	33.0	33.0	33.0	27.0	33.0
2	31.41531534010774	33.0	33.0	33.0	27.0	33.0
3	31.456550006535902	33.0	33.0	33.0	27.0	33.0
4	31.4151536625135	33.0	33.0	33.0	27.0	33.0
5	31.45236186885539	33.0	33.0	33.0	27.0	33.0
6	34.763591237762384	37.0	37.0	37.0	27.0	37.0
7	34.73841253242152	37.0	37.0	37.0	27.0	37.0
8	34.738073697463385	37.0	37.0	37.0	27.0	37.0
9	34.68538228151165	37.0	37.0	37.0	27.0	37.0
10-11	34.734074756967615	37.0	37.0	37.0	27.0	37.0
12-13	34.69226217913877	37.0	37.0	37.0	27.0	37.0
14-15	34.70589727624853	37.0	37.0	37.0	27.0	37.0
16-17	34.74314246204017	37.0	37.0	37.0	27.0	37.0
18-19	34.702999119373104	37.0	37.0	37.0	27.0	37.0
20-21	34.72376867032219	37.0	37.0	37.0	27.0	37.0
22-23	34.790962050484694	37.0	37.0	37.0	27.0	37.0
24-25	34.824773307373185	37.0	37.0	37.0	27.0	37.0
26-27	34.885901197790176	37.0	37.0	37.0	27.0	37.0
28-29	34.901539205096626	37.0	37.0	37.0	27.0	37.0
30-31	34.935930953347416	37.0	37.0	37.0	27.0	37.0
32-33	34.92082871806868	37.0	37.0	37.0	27.0	37.0
34-35	34.929728725636565	37.0	37.0	37.0	27.0	37.0
36-37	34.918564371762145	37.0	37.0	37.0	27.0	37.0
38-39	34.890949322674075	37.0	37.0	37.0	27.0	37.0
40-41	34.86444279709118	37.0	37.0	37.0	27.0	37.0
42-43	34.85434912728499	37.0	37.0	37.0	27.0	37.0
44-45	34.86732117426093	37.0	37.0	37.0	27.0	37.0
46-47	34.87322928634822	37.0	37.0	37.0	27.0	37.0
48-49	34.85041210586786	37.0	37.0	37.0	27.0	37.0
50-51	34.852415876051765	37.0	37.0	37.0	27.0	37.0
52-53	34.82583539156937	37.0	37.0	37.0	27.0	37.0
54-55	34.803401765381736	37.0	37.0	37.0	27.0	37.0
56-57	34.78436164869866	37.0	37.0	37.0	27.0	37.0
58-59	34.76523037337204	37.0	37.0	37.0	27.0	37.0
60-61	34.75415115823077	37.0	37.0	37.0	27.0	37.0
62-63	34.69360203920166	37.0	37.0	37.0	27.0	37.0
64-65	34.6646703497052	37.0	37.0	37.0	27.0	37.0
66-67	34.62099589958102	37.0	37.0	37.0	27.0	37.0
68-69	34.57550601647048	37.0	37.0	37.0	27.0	37.0
70-71	34.53972022896299	37.0	37.0	37.0	27.0	37.0
72-73	34.499120233090935	37.0	37.0	37.0	27.0	37.0
74-75	34.45071929329691	37.0	37.0	37.0	27.0	37.0
76-77	34.38401266589153	37.0	37.0	37.0	27.0	37.0
78-79	34.33491857641158	37.0	37.0	37.0	27.0	37.0
80-81	34.28010299206748	37.0	37.0	37.0	27.0	37.0
82-83	34.25320345233263	37.0	37.0	37.0	27.0	37.0
84-85	34.20405174371005	37.0	37.0	37.0	27.0	37.0
86-87	34.13594935707357	37.0	37.0	37.0	27.0	37.0
88-89	34.07615960674505	37.0	37.0	37.0	27.0	37.0
90-91	34.00354142730356	37.0	37.0	37.0	27.0	37.0
92-93	33.94881098169259	37.0	37.0	37.0	27.0	37.0
94-95	33.890288852501875	37.0	37.0	37.0	24.5	37.0
96-97	33.7837605176435	37.0	33.0	37.0	22.0	37.0
98-99	33.691103776375805	37.0	33.0	37.0	22.0	37.0
100-101	33.62245443787796	37.0	33.0	37.0	22.0	37.0
102-103	33.559118960309874	37.0	33.0	37.0	22.0	37.0
104-105	33.38087285261196	37.0	33.0	37.0	22.0	37.0
106-107	33.297432594203	37.0	33.0	37.0	22.0	37.0
108-109	33.202406588189966	37.0	33.0	37.0	18.0	37.0
110-111	33.09772550584447	37.0	33.0	37.0	14.0	37.0
112-113	33.000785168316696	37.0	33.0	37.0	14.0	37.0
114-115	32.88272010512483	37.0	33.0	37.0	14.0	37.0
116-117	32.79593879643071	37.0	33.0	37.0	14.0	37.0
118-119	32.66598527013918	37.0	33.0	37.0	14.0	37.0
120-121	32.54021128165613	37.0	33.0	37.0	14.0	37.0
122-123	32.426398855185035	37.0	33.0	37.0	14.0	37.0
124-125	30.848400595799134	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1015.0
1	48.0
2	36.0
3	21.0
4	31.0
5	16.0
6	16.0
7	14.0
8	10.0
9	21.0
10	22.0
11	17.0
12	26.0
13	16.0
14	48.0
15	127.0
16	248.0
17	425.0
18	987.0
19	1913.0
20	2882.0
21	3792.0
22	4399.0
23	5147.0
24	5745.0
25	6535.0
26	7759.0
27	9381.0
28	11249.0
29	13510.0
30	16360.0
31	19766.0
32	24833.0
33	33132.0
34	49017.0
35	90751.0
36	272089.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.67228704472091	19.65107993726892	12.52361473179703	24.153018286213136
2	17.712480078375638	22.733419344155976	35.777512539412044	23.77658803805634
3	19.94994920758661	26.98103347470124	28.255759204292435	24.813258113419717
4	14.716586690216884	19.052391186606734	33.68652911407252	32.54449300910386
5	15.711727687997682	37.268496156553184	30.800706586991623	16.219069568457513
6	29.790146064138433	35.53243597288934	18.356135539512223	16.32128242346001
7	26.67671218217249	33.112573636908124	21.260418770270107	18.950295410649282
8	25.923138662823312	33.37332033036739	20.408730398460357	20.29481060834894
9	26.789224866277394	18.09496850895195	19.704472609088132	35.41133401568253
10-11	25.45611618578126	26.980674827564783	25.353069312399455	22.210139674254506
12-13	26.176500288703785	25.90031131675682	25.070104528894976	22.85308386564442
14-15	24.683905342908012	26.163256817038345	23.227423320454523	25.925414519599123
16-17	24.230318429148415	28.007637952759573	23.962351861578494	23.799691756513518
18-19	24.335992038544756	27.07731287087558	25.32884552862355	23.25784956195611
20-21	26.242251957003266	25.670692329512878	24.8690086040029	23.218047109480956
22-23	26.046776688214614	25.465602891298687	24.678461997389416	23.809158423097283
24-25	24.785671244473143	26.292459507058947	24.727482442067657	24.19438680640025
26-27	24.462532460142565	26.454800652479044	24.75954492000924	24.32312196736915
28-29	24.24969636159735	26.304179736694433	25.191772756933258	24.25435114477496
30-31	24.687955782817227	26.074656147960678	25.090510062995	24.146878006227094
32-33	24.292707989410555	26.275341092374894	24.855972683300244	24.57597823491431
34-35	24.68513245943637	26.265870522226276	24.937526452666216	24.11147056567114
36-37	24.540463755573697	26.378953520144606	24.720528696179848	24.360054028101853
38-39	24.4539094394792	26.223984193975504	24.76337512413108	24.55873124241421
40-41	24.202099006863097	26.445900627702514	25.053888644212563	24.298111721221826
42-43	24.490617396108703	26.30188962220485	24.94718487405823	24.26030810762822
44-45	24.33557043509288	26.389038517393633	24.766500528751227	24.508890518762268
46-47	24.386558981779697	26.303995780397983	24.88479262672811	24.424652611094206
48-49	24.33204190602604	26.62523756372741	24.79747288218132	24.24524764806523
50-51	24.108056389989095	26.835928577277645	24.845815357695443	24.21019967503782
52-53	24.20968887479141	26.34384782998442	24.958626966942944	24.487836328281226
54-55	24.149296622010073	26.240425034929714	25.179647763679476	24.43063057938074
56-57	24.33426878165326	26.17252815825855	24.775906115004982	24.71729694508321
58-59	24.218071242397915	26.223918469791624	25.002930509012177	24.555079778798284
60-61	24.356097859506463	26.33669869405156	25.088328866968407	24.21887457947357
62-63	23.888157673580316	26.47821136403403	25.015924404116983	24.617706558268665
64-65	24.085174534872493	26.021711696801685	25.16093273752879	24.732181030797026
66-67	24.31968287932879	26.139092036895107	24.973080434540844	24.56814464923526
68-69	24.37060926523163	26.117032236150735	24.864759550023233	24.647598948594403
70-71	24.157595413036226	26.108141240000933	25.074677818554235	24.659585528408606
72-73	24.270877068352068	26.28670400829022	25.012996574766728	24.429422348590982
74-75	24.37089552817876	26.129551507376302	25.17004163422749	24.32951133021745
76-77	24.37261957513134	25.966868451407006	25.065733778627596	24.594778194834056
78-79	24.482913203903582	26.067709231352804	25.07043346322287	24.37894410152074
80-81	24.11710445779705	26.223157096181865	25.213838050328018	24.445900395693066
82-83	24.277675389673416	26.22987843878925	25.178997201902636	24.313448969634695
84-85	24.194464532133072	26.283861881362636	24.851511518362063	24.67016206814223
86-87	24.03482781295434	26.269609604795846	25.16294835034994	24.53261423189987
88-89	24.204261861100004	25.964985453693107	25.087984474747337	24.742768210459552
90-91	24.49675737401159	25.95798590912029	25.023075431452398	24.522181285415723
92-93	24.278106202926526	26.295220695954914	24.974405818582927	24.452267282535633
94-95	23.837151188440963	26.355867065571058	25.20034260740087	24.606639138587106
96-97	24.036382967174514	26.132702883635332	25.08992782511224	24.740986324077912
98-99	23.772521649218042	26.328551118004395	25.16436172504416	24.734565507733404
100-101	23.960260392656963	26.63311949886046	24.894296263425687	24.51232384505689
102-103	24.129326441299813	26.569685334840642	25.0835152128227	24.217473011036848
104-105	23.93808619400027	26.522941060896958	25.163212265565488	24.375760479537284
106-107	23.954225191309988	26.60503502215758	25.114449946620184	24.32628983991225
108-109	23.98280664019413	26.570600319876466	24.979145984998897	24.46744705493051
110-111	23.811718967432185	26.84490200014993	24.98365017668149	24.359728855736396
112-113	23.90052369554316	26.927239233567523	24.89673426974715	24.27550280114217
114-115	23.74400620382138	27.018848415656034	25.000883182905024	24.23626219761756
116-117	23.807443558101422	27.0926283028915	24.8805332533729	24.219394885634177
118-119	23.66666752828477	26.864735435853827	25.01837400601582	24.45022302984559
120-121	23.65700173801549	26.80688554000408	25.174857111344153	24.361255610636277
122-123	23.70397368226051	27.033929411521424	25.013053750432974	24.249043155785095
124-125	23.75192510387158	27.014367501574988	24.94081409809476	24.29289329645867
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1907.0
1	1195.0
2	379.5
3	247.0
4	193.5
5	165.0
6	151.5
7	142.5
8	131.0
9	128.0
10	140.0
11	144.0
12	134.0
13	132.5
14	153.0
15	166.5
16	183.0
17	216.0
18	243.5
19	280.5
20	323.5
21	383.0
22	482.5
23	662.0
24	855.0
25	1043.5
26	1344.0
27	1766.0
28	2193.5
29	2873.5
30	3884.0
31	4749.5
32	5767.0
33	6834.5
34	7714.0
35	8661.0
36	9640.5
37	10902.0
38	11846.0
39	12399.0
40	12935.0
41	13306.5
42	13928.0
43	15249.0
44	16907.0
45	18158.5
46	19745.5
47	21587.0
48	22922.5
49	24163.0
50	24461.5
51	23709.5
52	23558.0
53	24012.0
54	24114.0
55	23455.5
56	22361.5
57	20838.0
58	19466.5
59	17798.0
60	15340.0
61	12782.5
62	10382.5
63	8083.5
64	6121.0
65	4911.5
66	3905.0
67	3011.5
68	2449.5
69	2030.0
70	1728.0
71	1588.0
72	1428.5
73	1283.0
74	1003.0
75	807.5
76	653.0
77	457.5
78	324.0
79	195.5
80	126.5
81	93.0
82	77.0
83	65.5
84	39.0
85	19.0
86	10.5
87	5.0
88	2.0
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3073594264917338
2	0.280699823186631
3	0.27399192300018577
4	0.28379577711883647
5	0.29514760820358993
6	0.3441668787968435
7	0.3510467764239668
8	0.3524227559493915
9	0.34967079689854214
10-11	0.353626738034138
12-13	0.35956064973753193
14-15	0.34889680841549076
16-17	0.28680573233070294
18-19	0.2770018782120522
20-21	0.25791016229678504
22-23	0.2506002710679665
24-25	0.24002242846626445
26-27	0.25146025827135693
28-29	0.2331425308391411
30-31	0.23357252444083632
32-33	0.23950643614423014
34-35	0.23365852316117536
36-37	0.22970258202557944
38-39	0.2359804886103295
40-41	0.21886674326286026
42-43	0.2260906357713397
44-45	0.2173187662967575
46-47	0.21577078933065477
48-49	0.22299468183913426
50-51	0.22987457946625753
52-53	0.22634863193235683
54-55	0.2254026460086274
56-57	0.22187669847472669
58-59	0.22359667288150753
60-61	0.2283266025001548
62-63	0.22695062297473012
64-65	0.2318525500340555
66-67	0.24690232609338772
68-69	0.2439783696018603
70-71	0.2429463849577918
72-73	0.24827830561881237
74-75	0.2532662313984768
76-77	0.24303238367813085
78-79	0.2449243555255898
80-81	0.24234439391541854
82-83	0.23512050140693905
84-85	0.22505865112727122
86-87	0.2125028379577712
88-89	0.20433295952556224
90-91	0.21353482260183967
92-93	0.20536494416963075
94-95	0.19745306189843892
96-97	0.1853272423306341
98-99	0.19418511052555537
100-101	0.19375511692386016
102-103	0.19272313227979168
104-105	0.20261298511878145
106-107	0.19409911180521633
108-109	0.20295698000013762
110-111	0.19306712716114785
112-113	0.19005717194928137
114-115	0.19203514251707934
116-117	0.19212114123741839
118-119	0.18928318346623002
120-121	0.19685107085606565
122-123	0.19108915659334988
124-125	0.2133628251611616
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	581404.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.00924335738252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.98886976777523	71.2791176375874
2	8.536927995806948	13.831401550735537
3	1.8021145655689543	4.379638122001774
4	0.6617742304003832	2.1443931871259667
5	0.33391526715957504	1.3525111569037704
6	0.18564851040234648	0.9023547214871548
7	0.11524651248858381	0.6535222943394117
8	0.08207630053384682	0.5319151203056048
9	0.05558063136546949	0.40522904030180573
>10	0.22311327297149464	2.887648094629681
>50	0.010737572137233283	0.5749052058179352
>100	0.0034959517161906725	0.5206915244308109
>500	0.0	0.0
>1k	4.994216737406031E-4	0.5366723443331406
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1579	0.2715839588306926	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1074	0.18472525128826084	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	1.7199744067808272E-4
5	0.0	0.0	0.0	0.0	1.7199744067808272E-4
6	0.0	0.0	0.0	0.0	1.7199744067808272E-4
7	0.0	0.0	0.0	0.0	1.7199744067808272E-4
8	0.0	0.0	0.0	0.0	1.7199744067808272E-4
9	0.0	0.0	0.0	0.0	3.4399488135616543E-4
10-11	0.0	0.0	0.0	1.7199744067808272E-4	3.4399488135616543E-4
12-13	0.0	0.0	0.0	1.7199744067808272E-4	5.159923220342482E-4
14-15	0.0	0.0	0.0	1.7199744067808272E-4	6.019910423732895E-4
16-17	0.0	0.0	0.0	1.7199744067808272E-4	6.879897627123309E-4
18-19	0.0	0.0	0.0	1.7199744067808272E-4	6.879897627123309E-4
20-21	0.0	0.0	0.0	4.299936016952068E-4	8.599872033904136E-4
22-23	0.0	0.0	0.0	0.0017199744067808273	8.599872033904136E-4
24-25	0.0	0.0	0.0	0.004557932177969192	8.599872033904136E-4
26-27	0.0	0.0	0.0	0.007395889949157557	9.45985923729455E-4
28-29	0.0	0.0	0.0	0.012383815728821955	0.0010319846440684963
30-31	0.0	0.0	0.0	0.02347765065255829	0.0010319846440684963
32-33	0.0	0.0	0.0	0.04815928338986315	0.0010319846440684963
34-35	0.0	0.0	0.0	0.07980681247463038	0.0010319846440684963
36-37	0.0	0.0	0.0	0.12908407922890108	0.0010319846440684963
38-39	0.0	0.0	0.0	0.1998610260679321	0.0010319846440684963
40-41	0.0	0.0	0.0	0.27338993195781247	0.0010319846440684963
42-43	0.0	0.0	0.0	0.3383189658137887	0.0010319846440684963
44-45	0.0	0.0	0.0	0.40814992672909023	0.0010319846440684963
46-47	0.0	0.0	0.0	0.49991056133084744	0.0010319846440684963
48-49	0.0	0.0	0.0	0.6001850692461697	0.0010319846440684963
50-51	0.0	0.0	0.0	0.6950416577801323	0.0010319846440684963
52-53	0.0	0.0	0.0	0.7830183486869715	0.0010319846440684963
54-55	0.0	0.0	0.0	0.858869220026006	0.0010319846440684963
56-57	0.0	0.0	0.0	0.9549297906447152	0.0010319846440684963
58-59	0.0	0.0	0.0	1.0680181078905546	0.0010319846440684963
60-61	0.0	0.0	0.0	1.1745705223906269	0.0010319846440684963
62-63	0.0	0.0	0.0	1.2739850431025586	0.001203982084746579
64-65	0.0	0.0	0.0	1.3779574959924596	0.001203982084746579
66-67	0.0	0.0	0.0	1.4847679066535489	0.001203982084746579
68-69	0.0	0.0	0.0	1.6093800524248199	0.001203982084746579
70-71	0.0	0.0	0.0	1.755147883399495	0.001203982084746579
72-73	0.0	0.0	0.0	1.8848339536707694	0.001203982084746579
74-75	0.0	0.0	0.0	2.0060921493488175	0.001203982084746579
76-77	0.0	0.0	0.0	2.136724205543821	0.001203982084746579
78-79	0.0	0.0	0.0	2.2866199750947707	0.001203982084746579
80-81	0.0	0.0	0.0	2.4494155526965757	0.001203982084746579
82-83	0.0	0.0	0.0	2.6146190944678747	0.001203982084746579
84-85	0.0	0.0	0.0	2.778102661832392	0.001203982084746579
86-87	0.0	0.0	0.0	2.950788092273187	0.0012899808050856204
88-89	0.0	0.0	0.0	3.131385404985174	0.0015479769661027444
90-91	0.0	0.0	0.0	3.3260865078327635	0.0015479769661027444
92-93	0.0	0.0	0.0	3.5386753445108736	0.0015479769661027444
94-95	0.0	0.0	0.0	3.7528981568754256	0.0018919718474589098
96-97	0.0	0.0	0.0	3.955339144553529	0.0018919718474589098
98-99	0.0	0.0	0.0	4.178075830231647	0.0020639692881369927
100-101	0.0	0.0	0.0	4.420248226706387	0.0020639692881369927
102-103	0.0	0.0	0.0	4.664914586070959	0.0020639692881369927
104-105	0.0	0.0	0.0	4.92953264855419	0.0020639692881369927
106-107	0.0	0.0	0.0	5.1989666393764065	0.0020639692881369927
108-109	0.0	0.0	0.0	5.46324070697828	0.0022359667288150754
110-111	0.0	0.0	0.0	5.744800517368301	0.0022359667288150754
112-113	0.0	0.0	0.0	6.0427860833430795	0.0022359667288150754
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1650	0.0	46.57619	1
GTATCAA	2445	0.0	39.472477	1
TATCAAC	3095	0.0	30.61045	2
ATCAACG	3005	0.0	30.535812	3
TCAACGC	3025	0.0	30.530897	4
CAACGCA	3035	0.0	30.430302	5
AACGCAG	3065	0.0	30.13245	6
TCTAGAC	180	2.1644155E-8	29.792248	3
TTCGCGA	40	0.005370286	29.740858	54-55
ACGCAGA	3530	0.0	26.50075	7
CGCAGAG	3625	0.0	25.80625	8
GTACATG	5050	0.0	24.065619	1
GCAGAGT	3905	0.0	23.650696	9
CATGGGG	2530	0.0	23.551182	4
ACATGGG	5065	0.0	23.057375	3
TACATGG	5160	0.0	22.979292	2
CAGAGTA	3945	0.0	21.22084	10-11
ATGGGGG	1210	0.0	20.189787	5
AGAGTAC	3725	0.0	19.994795	10-11
CATGGGT	1440	0.0	19.861498	4
>>END_MODULE