FastQCFastQC Report
Sun 1 Jan 2017
ERR1142662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581404
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30100.517712296441029No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28220.4853767775935494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16270.2798398359832406No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7470.1284820881865278No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19150.066.1999741
GTATCAA31450.044.472771
ATCAACG41400.033.6144683
TCAACGC42550.033.1252754
TATCAAC42200.033.1181532
CAACGCA43000.032.7786145
AACGCAG44900.031.5246
GTGGTAT8950.030.590161
TAAGACG400.00537469229.73587450-51
ACGCAGA49550.028.445627
CGCAGAG51550.027.4573758
TGGTATC9800.026.7016012
GCAGAGT55150.025.6650529
CATGGGT11500.025.3401364
CAGAGTA54000.025.00016210-11
GAGTACT25650.024.5770212-13
ATGGGTA5100.022.1561415
GTACATG42750.022.1364521
AGAGTAC52750.021.92844610-11
TACATGG44050.021.7365532
Produced by FastQC (version 0.11.5)