##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142662_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	581404
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.89268219688891	27.0	14.0	33.0	14.0	33.0
2	29.647317527915185	33.0	27.0	33.0	27.0	33.0
3	29.132683985662293	33.0	27.0	33.0	14.0	33.0
4	30.30804053635682	33.0	33.0	33.0	27.0	33.0
5	31.320957544151742	33.0	33.0	33.0	27.0	33.0
6	33.07735412897056	37.0	33.0	37.0	27.0	37.0
7	35.18236888635097	37.0	37.0	37.0	33.0	37.0
8	34.819414727108864	37.0	37.0	37.0	27.0	37.0
9	35.60739004203617	37.0	37.0	37.0	33.0	37.0
10-11	35.771344022400946	37.0	37.0	37.0	33.0	37.0
12-13	35.8558498049549	37.0	37.0	37.0	33.0	37.0
14-15	35.952619005029206	37.0	37.0	37.0	33.0	37.0
16-17	35.98160315374507	37.0	37.0	37.0	33.0	37.0
18-19	36.02625454933231	37.0	37.0	37.0	35.0	37.0
20-21	36.06215299516343	37.0	37.0	37.0	37.0	37.0
22-23	36.06861923894572	37.0	37.0	37.0	37.0	37.0
24-25	36.06739203720649	37.0	37.0	37.0	37.0	37.0
26-27	35.966082964685484	37.0	37.0	37.0	35.0	37.0
28-29	35.93947582059979	37.0	37.0	37.0	33.0	37.0
30-31	35.972881163528285	37.0	37.0	37.0	35.0	37.0
32-33	35.92598348136579	37.0	37.0	37.0	33.0	37.0
34-35	35.86093146933973	37.0	37.0	37.0	33.0	37.0
36-37	35.7948715523113	37.0	37.0	37.0	33.0	37.0
38-39	35.74162458462618	37.0	37.0	37.0	33.0	37.0
40-41	35.671048014805535	37.0	37.0	37.0	33.0	37.0
42-43	35.59565465665871	37.0	37.0	37.0	33.0	37.0
44-45	35.51392663277171	37.0	37.0	37.0	33.0	37.0
46-47	35.39366000233916	37.0	37.0	37.0	33.0	37.0
48-49	35.27547454093883	37.0	37.0	37.0	33.0	37.0
50-51	35.191483030732506	37.0	37.0	37.0	33.0	37.0
52-53	35.130828133277376	37.0	37.0	37.0	33.0	37.0
54-55	35.06864417857462	37.0	37.0	37.0	33.0	37.0
56-57	35.02998431383341	37.0	37.0	37.0	33.0	37.0
58-59	34.9791496102538	37.0	37.0	37.0	27.0	37.0
60-61	34.93656476391631	37.0	37.0	37.0	27.0	37.0
62-63	34.91541337864892	37.0	37.0	37.0	27.0	37.0
64-65	34.89660287854917	37.0	37.0	37.0	27.0	37.0
66-67	34.870178911737796	37.0	37.0	37.0	27.0	37.0
68-69	34.823054192953606	37.0	37.0	37.0	27.0	37.0
70-71	34.764861438861786	37.0	37.0	37.0	27.0	37.0
72-73	34.7307466064905	37.0	37.0	37.0	27.0	37.0
74-75	34.62061578523712	37.0	37.0	37.0	27.0	37.0
76-77	34.56550006535903	37.0	37.0	37.0	27.0	37.0
78-79	34.558773245454105	37.0	37.0	37.0	27.0	37.0
80-81	34.51951740958094	37.0	37.0	37.0	27.0	37.0
82-83	34.4852881989116	37.0	37.0	37.0	27.0	37.0
84-85	34.362082992205075	37.0	37.0	37.0	27.0	37.0
86-87	34.23563821370338	37.0	37.0	37.0	27.0	37.0
88-89	34.17732506140309	37.0	37.0	37.0	27.0	37.0
90-91	34.15040918191137	37.0	37.0	37.0	27.0	37.0
92-93	34.037792137652986	37.0	37.0	37.0	27.0	37.0
94-95	34.079514416825475	37.0	37.0	37.0	27.0	37.0
96-97	34.07474750775708	37.0	37.0	37.0	27.0	37.0
98-99	34.0072281924445	37.0	37.0	37.0	27.0	37.0
100-101	33.917846282447314	37.0	37.0	37.0	22.0	37.0
102-103	33.8142745835942	37.0	33.0	37.0	22.0	37.0
104-105	33.723398015837525	37.0	33.0	37.0	22.0	37.0
106-107	33.62141901328508	37.0	33.0	37.0	22.0	37.0
108-109	33.51786709413764	37.0	33.0	37.0	22.0	37.0
110-111	33.46561513164684	37.0	33.0	37.0	22.0	37.0
112-113	33.382000295835596	37.0	33.0	37.0	22.0	37.0
114-115	33.295894077096136	37.0	33.0	37.0	18.0	37.0
116-117	33.193900454761234	37.0	33.0	37.0	14.0	37.0
118-119	33.08287610680353	37.0	33.0	37.0	14.0	37.0
120-121	33.013482879374756	37.0	33.0	37.0	14.0	37.0
122-123	32.89053222888043	37.0	33.0	37.0	14.0	37.0
124-125	31.62343740325144	37.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	2.0
17	10.0
18	73.0
19	292.0
20	1206.0
21	2879.0
22	4606.0
23	6274.0
24	5992.0
25	4840.0
26	4963.0
27	5819.0
28	7266.0
29	9591.0
30	12620.0
31	17327.0
32	24341.0
33	35649.0
34	58103.0
35	119280.0
36	260269.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.39618755258978	16.060253050422524	13.14051990109249	26.403039495895204
2	18.231040722114056	20.53150649118341	37.382095754415175	23.855357032287362
3	20.51809069081052	24.450640174474202	28.80733534685004	26.22393378786524
4	14.483169045698393	17.439314481105058	34.91474873537794	33.162767737818605
5	16.340702362768166	33.77936258927466	32.2985041764714	17.58143087148578
6	29.99661162127902	34.11426576241101	18.647950890868405	17.241171725441564
7	26.49087381579762	31.789426973326634	22.35829130862533	19.361407902250413
8	26.198822161526238	31.26431878693645	21.300679045895798	21.23618000564152
9	27.10370069693363	15.891359536570096	20.647776761081797	36.35716300541448
10-11	25.828081678144628	24.780445266974425	26.426374775543337	22.965098279337603
12-13	27.091488878645485	23.9036883131179	26.367637649551774	22.63718515868484
14-15	25.172427434279776	24.135626861872296	25.094598592372947	25.59734711147498
16-17	23.92054406230435	26.209400004127936	25.969979566704048	23.90007636686366
18-19	23.64887410475332	24.821724652737167	28.411913230731127	23.117488011778384
20-21	25.397122981490384	23.62252870439568	28.2300238819884	22.75032443212553
22-23	25.097350551423798	23.36473433275313	28.297190937798845	23.24072417802423
24-25	23.97463725739761	24.28225468005036	28.277497230841202	23.465610831710823
26-27	23.67940365047368	24.412284745202992	28.412945215375196	23.49536638894813
28-29	23.407389698041293	24.34890368831312	28.768033931655097	23.475672681990492
30-31	23.815195629889025	24.097873423643456	28.665867451892318	23.4210634945752
32-33	23.583879354011454	24.22379638237472	28.351996505008998	23.840327758604822
34-35	23.950340813514895	24.25993673923251	28.455391527047503	23.33433092020509
36-37	23.767834260700557	24.365534619320773	28.184796910878145	23.681834209100526
38-39	23.668594781190823	24.38419874887555	28.075480265636045	23.87172620429758
40-41	23.567136440139357	24.48177137308763	28.375011502813567	23.576080683959443
42-43	23.8799167177649	24.3501637866757	28.164256234772655	23.605663260786745
44-45	23.69573892074675	24.56088902342957	27.798063990147952	23.94530806567573
46-47	23.767720097282087	24.51745952026309	27.72049287756148	23.994327504893345
48-49	23.865074887685672	24.811060811415125	27.533092307586465	23.790771993312738
50-51	23.7279069287449	25.071808931483098	27.271570199035438	23.92871394073656
52-53	23.86086095038906	24.75352766750831	27.289887926467653	24.095723455634978
54-55	23.818783028968742	24.71523600259717	27.321519945304672	24.144461023129416
56-57	23.900420361745017	24.6861046707625	26.964382769984386	24.4490921975081
58-59	23.747739287253065	24.765801769354407	27.24370633911164	24.242752604280884
60-61	23.883736609999243	24.729190029652358	27.453543491272846	23.93352986907555
62-63	23.487970498998976	24.778639293847306	27.43565575744233	24.29773444971139
64-65	23.511362150931195	24.460100033711498	27.706895721391668	24.321642093965643
66-67	23.679145654312663	24.661251040584517	27.47117322894235	24.188430076160465
68-69	23.673727734931305	24.597525988813288	27.379412594340597	24.349333681914814
70-71	23.478859128221224	24.50844731874492	27.707033483976108	24.305660069057744
72-73	23.668330170011018	24.78210055495022	27.503446401681447	24.046122873357316
74-75	23.587006581071137	24.718227716252102	27.653066934098902	24.04169876857786
76-77	23.617707598895667	24.60031525400675	27.503114230937765	24.278862916159824
78-79	23.644114513145414	24.79555831135336	27.472198596320435	24.08812857918079
80-81	23.51170614581255	24.848556253482947	27.694941899264542	23.944795701439965
82-83	23.514108998593056	24.817940082765453	27.598890268695325	24.069060649946163
84-85	23.391520338187753	25.015906361253048	27.29299129993069	24.299582000628504
86-87	23.307568940362138	24.99941829751626	27.48919110607065	24.203821656050955
88-89	23.41321092754458	24.676469016034723	27.40697568150584	24.50334437491486
90-91	23.677956312196415	24.752376386171466	27.254995295861477	24.31467200577064
92-93	23.58150377053897	24.951629937579792	27.15037877678444	24.3164875150968
94-95	23.197098019250216	24.986756014833265	27.294133430570554	24.52201253534596
96-97	23.41237762382096	24.736671918321857	27.237600704501517	24.61334975335567
98-99	23.13666572641399	24.88011778384738	27.078417073153954	24.90479941658468
100-101	23.37247421758364	25.25412621860187	26.754889887238477	24.61850967657601
102-103	23.650356422002965	25.056092713580156	26.958386043427673	24.33516482098921
104-105	23.398752956353473	25.22193076757687	26.728101483551924	24.651214792517738
106-107	23.464443408575278	25.226491264584112	26.72648524431127	24.58258008252934
108-109	23.502147591205972	25.21884444627926	26.549714543243386	24.729293419271382
110-111	23.29819540039097	25.456095071085727	26.50808298595314	24.737626542570162
112-113	23.38113687867933	25.44083182469071	26.447157373284767	24.730873923345186
114-115	23.31229725034487	25.487115184164626	26.53393121906079	24.666656346429715
116-117	23.284465699633902	25.63981283125831	26.422061900531563	24.653659568576224
118-119	23.138406286926145	25.460829691243674	26.522152677086396	24.87861134474378
120-121	23.096934317617354	25.435755515957926	26.707504592331667	24.759805574093058
122-123	23.141825649634335	25.632176593212293	26.497667714704402	24.728330042448967
124-125	23.122039483868303	25.496902315437513	26.486492994520145	24.89456520617404
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	241.0
1	231.5
2	370.5
3	486.0
4	361.5
5	304.5
6	451.5
7	933.0
8	1497.0
9	1748.5
10	1574.5
11	1219.5
12	1079.0
13	1043.0
14	988.5
15	941.5
16	907.5
17	879.5
18	886.0
19	855.0
20	820.5
21	834.5
22	878.5
23	945.0
24	1048.0
25	1196.0
26	1411.0
27	1727.5
28	2092.0
29	2663.0
30	3476.0
31	4267.0
32	5245.5
33	6209.0
34	7158.5
35	8204.0
36	9170.5
37	10170.0
38	11038.5
39	11775.0
40	12259.5
41	12765.5
42	13469.5
43	14635.0
44	16183.5
45	17636.0
46	19318.0
47	20725.5
48	22291.5
49	23862.0
50	24109.5
51	23428.0
52	23377.0
53	24040.5
54	24104.0
55	23463.5
56	22540.0
57	20805.5
58	19240.0
59	17597.0
60	15155.0
61	12686.5
62	10346.0
63	8159.0
64	6205.0
65	4989.5
66	3965.0
67	2917.5
68	2348.0
69	2010.0
70	1690.5
71	1500.5
72	1336.5
73	1273.0
74	1019.0
75	765.5
76	606.0
77	444.5
78	326.0
79	205.0
80	132.5
81	94.5
82	64.5
83	45.0
84	28.0
85	16.5
86	10.5
87	3.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04385934737291109
2	0.0
3	0.0
4	8.599872033904136E-4
5	0.0089438669152603
6	8.599872033904136E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.019910423732895E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	8.599872033904136E-5
34-35	1.7199744067808272E-4
36-37	0.0015479769661027444
38-39	0.001203982084746579
40-41	0.004213937296613026
42-43	0.0012899808050856204
44-45	3.4399488135616543E-4
46-47	3.4399488135616543E-4
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.5799616101712403E-4
56-57	0.0
58-59	9.459859237294549E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0011179833644075375
72-73	8.599872033904136E-5
74-75	0.09829653734752426
76-77	0.10362845800854484
78-79	6.019910423732895E-4
80-81	0.0
82-83	3.4399488135616543E-4
84-85	0.11377630700855172
86-87	0.2082029019408191
88-89	0.253696225000172
90-91	0.09158863716107904
92-93	0.16907348418655532
94-95	0.0013759795254246617
96-97	0.0
98-99	0.0
100-101	0.0
102-103	8.599872033904136E-5
104-105	0.004987925779664399
106-107	0.006019910423732895
108-109	0.009459859237294548
110-111	0.0049019270593253575
112-113	0.0054179193813596056
114-115	0.0037839436949178196
116-117	6.019910423732895E-4
118-119	0.001203982084746579
120-121	0.0
122-123	0.0
124-125	3.4399488135616543E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	581404.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.92195690927677
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.75779751803272	69.26017114171215
2	8.591454177728174	13.561087528053777
3	1.8710939814601462	4.430111957340143
4	0.6923454918326809	2.185650442910035
5	0.35897327070209084	1.416543650096628
6	0.1848984289994187	0.8755527507651054
7	0.134277253610563	0.7418196536344217
8	0.08198215313054755	0.5176153565359042
9	0.061499645741606795	0.436830515203939
>10	0.24442898055099163	3.190031345194032
>50	0.014166068030600274	0.7398854921858998
>100	0.005818203362527619	0.8970059521019371
>500	5.059307271759294E-4	0.258540553743944
>1k	7.588960907638941E-4	1.4891536605220685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3010	0.517712296441029	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2822	0.4853767775935494	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1627	0.2798398359832406	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	747	0.1284820881865278	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.7199744067808272E-4	0.0
12-13	0.0	0.0	0.0	1.7199744067808272E-4	0.0
14-15	0.0	0.0	0.0	1.7199744067808272E-4	0.0
16-17	0.0	0.0	0.0	1.7199744067808272E-4	0.0
18-19	0.0	0.0	0.0	1.7199744067808272E-4	1.7199744067808272E-4
20-21	0.0	0.0	0.0	4.299936016952068E-4	1.7199744067808272E-4
22-23	0.0	0.0	0.0	0.0017199744067808273	2.579961610171241E-4
24-25	0.0	0.0	0.0	0.004557932177969192	3.4399488135616543E-4
26-27	0.0	0.0	0.0	0.007309891228818515	3.4399488135616543E-4
28-29	0.0	0.0	0.0	0.012211818288143873	3.4399488135616543E-4
30-31	0.0	0.0	0.0	0.02339165193221925	3.4399488135616543E-4
32-33	0.0	0.0	0.0	0.04772928978816795	3.4399488135616543E-4
34-35	0.0	0.0	0.0	0.07851683166954476	3.4399488135616543E-4
36-37	0.0	0.0	0.0	0.12762210098313737	3.4399488135616543E-4
38-39	0.0	0.0	0.0	0.19831304910182934	3.4399488135616543E-4
40-41	0.0	0.0	0.0	0.27209995115272684	3.4399488135616543E-4
42-43	0.0	0.0	0.0	0.33677098884768597	3.4399488135616543E-4
44-45	0.0	0.0	0.0	0.40728993952569986	3.4399488135616543E-4
46-47	0.0	0.0	0.0	0.499136572847796	3.4399488135616543E-4
48-49	0.0	0.0	0.0	0.5995830782037963	3.4399488135616543E-4
50-51	0.0	0.0	0.0	0.6949556590597932	3.4399488135616543E-4
52-53	0.0	0.0	0.0	0.7843943282123962	3.4399488135616543E-4
54-55	0.0	0.0	0.0	0.8599872033904136	3.4399488135616543E-4
56-57	0.0	0.0	0.0	0.9573377548142084	3.4399488135616543E-4
58-59	0.0	0.0	0.0	1.0703400733397088	5.159923220342482E-4
60-61	0.0	0.0	0.0	1.176892487839781	0.0010319846440684963
62-63	0.0	0.0	0.0	1.2761350111110348	0.0010319846440684963
64-65	0.0	0.0	0.0	1.3792474767975453	0.0010319846440684963
66-67	0.0	0.0	0.0	1.486659878501008	0.0010319846440684963
68-69	0.0	0.0	0.0	1.6109280293909225	0.0010319846440684963
70-71	0.0	0.0	0.0	1.7567818590859368	0.0010319846440684963
72-73	0.0	0.0	0.0	1.8866399267978893	0.001203982084746579
74-75	0.0	0.0	0.0	2.0075541275945814	0.001203982084746579
76-77	0.0	0.0	0.0	2.1381001850692463	0.001203982084746579
78-79	0.0	0.0	0.0	2.287909955899856	0.001203982084746579
80-81	0.0	0.0	0.0	2.45173751814573	0.001203982084746579
82-83	0.0	0.0	0.0	2.614791091908552	0.0013759795254246617
84-85	0.0	0.0	0.0	2.7780166631120533	0.0013759795254246617
86-87	0.0	0.0	0.0	2.9499281050697967	0.001461978245763703
88-89	0.0	0.0	0.0	3.1290634395360195	0.0015479769661027444
90-91	0.0	0.0	0.0	3.3235065462225926	0.0015479769661027444
92-93	0.0	0.0	0.0	3.5360953829007027	0.0015479769661027444
94-95	0.0	0.0	0.0	3.751436178629662	0.0015479769661027444
96-97	0.0	0.0	0.0	3.9540491637484436	0.0015479769661027444
98-99	0.0	0.0	0.0	4.176699850706221	0.0015479769661027444
100-101	0.0	0.0	0.0	4.419560236943674	0.0015479769661027444
102-103	0.0	0.0	0.0	4.664570591189603	0.0016339756864417858
104-105	0.0	0.0	0.0	4.928844658791477	0.001719974406780827
106-107	0.0	0.0	0.0	5.197418662410303	0.001719974406780827
108-109	0.0	0.0	0.0	5.460832742808787	0.001719974406780827
110-111	0.0	0.0	0.0	5.741876560876774	0.001719974406780827
112-113	0.0	0.0	0.0	6.042270091021045	0.001719974406780827
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1915	0.0	66.199974	1
GTATCAA	3145	0.0	44.47277	1
ATCAACG	4140	0.0	33.614468	3
TCAACGC	4255	0.0	33.125275	4
TATCAAC	4220	0.0	33.118153	2
CAACGCA	4300	0.0	32.778614	5
AACGCAG	4490	0.0	31.524	6
GTGGTAT	895	0.0	30.59016	1
TAAGACG	40	0.005374692	29.735874	50-51
ACGCAGA	4955	0.0	28.44562	7
CGCAGAG	5155	0.0	27.457375	8
TGGTATC	980	0.0	26.701601	2
GCAGAGT	5515	0.0	25.665052	9
CATGGGT	1150	0.0	25.340136	4
CAGAGTA	5400	0.0	25.000162	10-11
GAGTACT	2565	0.0	24.57702	12-13
ATGGGTA	510	0.0	22.156141	5
GTACATG	4275	0.0	22.136452	1
AGAGTAC	5275	0.0	21.928446	10-11
TACATGG	4405	0.0	21.736553	2
>>END_MODULE