FastQCFastQC Report
Sun 1 Jan 2017
ERR1142654_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142654_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448822
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20360.4536319520879102No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9120.20319859543427016No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG551.2980578E-554.170492
ATCACGC758.1100574E-539.725023
GGTATCA17800.032.4682271
TCACGCA953.254948E-431.3618624
GTATCAA24100.030.6557181
ATCAACG29200.024.8961643
TATCAAC30000.024.828142
TCAACGC30050.024.1919464
GTACATG45350.024.1739371
AACGCAG29350.024.1652626
CAACGCA30300.023.992345
CTAGAAC1501.6676424E-423.8350143
GTATCAC1751.7293716E-523.8323521
ACATGGG44550.023.674513
TATTCTC3052.6921043E-1023.4442795
TACATGG46550.023.425392
CATGGGG16050.022.2757154
CATGGGT7450.021.5954844
ACGCAGA33200.021.3629637
CACGCAG1703.8896452E-421.0308975
Produced by FastQC (version 0.11.5)