##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142654_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448822
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.185276122828203	33.0	33.0	33.0	27.0	33.0
2	31.320810922815728	33.0	33.0	33.0	27.0	33.0
3	31.366592992322122	33.0	33.0	33.0	27.0	33.0
4	31.29040911541769	33.0	33.0	33.0	27.0	33.0
5	31.387182446493263	33.0	33.0	33.0	27.0	33.0
6	34.67135969270669	37.0	37.0	37.0	27.0	37.0
7	34.67712589846309	37.0	37.0	37.0	27.0	37.0
8	34.60185106790665	37.0	37.0	37.0	27.0	37.0
9	34.501548498068274	37.0	37.0	37.0	27.0	37.0
10-11	34.60078828577922	37.0	37.0	37.0	27.0	37.0
12-13	34.56601949102317	37.0	37.0	37.0	27.0	37.0
14-15	34.57578282704502	37.0	37.0	37.0	27.0	37.0
16-17	34.62813431605403	37.0	37.0	37.0	27.0	37.0
18-19	34.59269376278346	37.0	37.0	37.0	27.0	37.0
20-21	34.616745614074176	37.0	37.0	37.0	27.0	37.0
22-23	34.70081012071601	37.0	37.0	37.0	27.0	37.0
24-25	34.76513183400101	37.0	37.0	37.0	27.0	37.0
26-27	34.845934468452974	37.0	37.0	37.0	27.0	37.0
28-29	34.87607670746978	37.0	37.0	37.0	27.0	37.0
30-31	34.9055360476982	37.0	37.0	37.0	27.0	37.0
32-33	34.900452740730174	37.0	37.0	37.0	27.0	37.0
34-35	34.919294285930725	37.0	37.0	37.0	27.0	37.0
36-37	34.898758305074175	37.0	37.0	37.0	27.0	37.0
38-39	34.8706837008881	37.0	37.0	37.0	27.0	37.0
40-41	34.85188114664611	37.0	37.0	37.0	27.0	37.0
42-43	34.834685019896526	37.0	37.0	37.0	27.0	37.0
44-45	34.85939971748266	37.0	37.0	37.0	27.0	37.0
46-47	34.85878700241967	37.0	37.0	37.0	27.0	37.0
48-49	34.844367031919106	37.0	37.0	37.0	27.0	37.0
50-51	34.843627317733976	37.0	37.0	37.0	27.0	37.0
52-53	34.82354251796926	37.0	37.0	37.0	27.0	37.0
54-55	34.80947903623263	37.0	37.0	37.0	27.0	37.0
56-57	34.78317127948273	37.0	37.0	37.0	27.0	37.0
58-59	34.76953335620803	37.0	37.0	37.0	27.0	37.0
60-61	34.76603531021206	37.0	37.0	37.0	27.0	37.0
62-63	34.700617393978014	37.0	37.0	37.0	27.0	37.0
64-65	34.66384780603447	37.0	37.0	37.0	27.0	37.0
66-67	34.613238655859114	37.0	37.0	37.0	27.0	37.0
68-69	34.58249818413536	37.0	37.0	37.0	27.0	37.0
70-71	34.53870910962475	37.0	37.0	37.0	27.0	37.0
72-73	34.477154640369676	37.0	37.0	37.0	27.0	37.0
74-75	34.43601806506811	37.0	37.0	37.0	27.0	37.0
76-77	34.36873972309735	37.0	37.0	37.0	27.0	37.0
78-79	34.316467330032836	37.0	37.0	37.0	27.0	37.0
80-81	34.26033260401674	37.0	37.0	37.0	27.0	37.0
82-83	34.23823698481803	37.0	37.0	37.0	27.0	37.0
84-85	34.18340567084501	37.0	37.0	37.0	27.0	37.0
86-87	34.113846914812555	37.0	37.0	37.0	27.0	37.0
88-89	34.062766531052404	37.0	37.0	37.0	27.0	37.0
90-91	34.008219294063124	37.0	37.0	37.0	27.0	37.0
92-93	33.95477828626939	37.0	37.0	37.0	27.0	37.0
94-95	33.89308010748136	37.0	37.0	37.0	22.0	37.0
96-97	33.796538494102336	37.0	37.0	37.0	22.0	37.0
98-99	33.710151240358094	37.0	33.0	37.0	22.0	37.0
100-101	33.6498132890099	37.0	33.0	37.0	22.0	37.0
102-103	33.56537335513856	37.0	33.0	37.0	22.0	37.0
104-105	33.39764873379647	37.0	33.0	37.0	22.0	37.0
106-107	33.31970914972973	37.0	33.0	37.0	18.0	37.0
108-109	33.22004825966641	37.0	33.0	37.0	14.0	37.0
110-111	33.131597827200984	37.0	33.0	37.0	14.0	37.0
112-113	33.01436649718596	37.0	33.0	37.0	14.0	37.0
114-115	32.90229199994653	37.0	33.0	37.0	14.0	37.0
116-117	32.79722139289072	37.0	33.0	37.0	14.0	37.0
118-119	32.66950706516169	37.0	33.0	37.0	14.0	37.0
120-121	32.54989283056535	37.0	33.0	37.0	14.0	37.0
122-123	32.43721453048202	37.0	33.0	37.0	14.0	37.0
124-125	30.880904902166115	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	852.0
1	47.0
2	29.0
3	21.0
4	11.0
5	18.0
6	12.0
7	10.0
8	20.0
9	11.0
10	21.0
11	16.0
12	13.0
13	14.0
14	32.0
15	163.0
16	245.0
17	444.0
18	961.0
19	1863.0
20	2832.0
21	3619.0
22	3898.0
23	4218.0
24	4645.0
25	5015.0
26	5679.0
27	6706.0
28	8176.0
29	10063.0
30	11875.0
31	14236.0
32	18144.0
33	24306.0
34	35911.0
35	67940.0
36	216756.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.743251671573525	20.405156784766607	12.398593441574844	23.452998102085022
2	16.936633725321926	23.681787503743333	36.13312415355941	23.24845461737533
3	19.404469273743015	28.835530726256987	28.2531843575419	23.5068156424581
4	13.830326951056795	20.0014750679984	34.36506144999184	31.803136530952962
5	14.492118697864045	39.273516500219046	30.972891539334984	15.261473262581921
6	28.63522518896194	37.22259492821682	18.428373361957153	15.713806520864082
7	25.565937727839977	34.96468620145191	21.33901693899368	18.130359131714428
8	25.38205028996236	34.88471955395223	20.326886991839007	19.4063431642464
9	26.5483440272488	19.18173096061851	19.73905206211044	34.53087295002225
10-11	24.46170757372272	28.600870213796163	25.43815410008018	21.499268112400937
12-13	25.15083837619063	27.661549438640794	25.247352569816222	21.940259615352357
14-15	24.07370340841363	27.584656827170694	23.51392785537529	24.82771190904039
16-17	23.362185332189085	29.611291389544448	24.036553314739326	22.98996996352714
18-19	23.554932831876897	28.46413323903092	25.721541956194073	22.25939197289811
20-21	25.648988674675643	26.91099488137343	25.12305035289702	22.31696609105391
22-23	25.20818695551641	27.02962035412168	24.85017799914882	22.912014691213088
24-25	24.035134356298293	27.57537646346612	25.09890473201341	23.29058444822217
26-27	23.8032898220154	27.443183214446087	25.154761931357417	23.5987650321811
28-29	23.706194674451798	27.517193778744716	25.425684233098277	23.35092731370521
30-31	24.16903346426573	27.240602519843687	25.456876631303377	23.133487384587205
32-33	23.452738896756927	27.48904307267537	25.258007157185553	23.80021087338215
34-35	23.752831174864973	27.554535017221582	25.399938350748503	23.29269545716494
36-37	23.839097455507112	27.76561718247445	24.958343756700735	23.436941605317703
38-39	23.835190498581017	27.47908385556217	25.03146775434713	23.65425789150968
40-41	23.55482599398112	27.673908309743556	25.29304366761026	23.47822202866507
42-43	23.63388237501382	27.43458795071468	25.361303226685454	23.570226447586045
44-45	23.560658425001478	27.603176216122055	25.17779954016109	23.658365818715378
46-47	23.66838929262159	27.51794464072752	25.253257281705178	23.560408784945707
48-49	23.491495555979913	27.606415827166202	25.346716204732022	23.555372412121862
50-51	23.3298600990151	27.862710083212438	25.19580218869602	23.61162762907644
52-53	23.374762542396162	27.50588269454772	25.279337251779328	23.84001751127679
54-55	23.287539672081355	27.370330085117693	25.42542095400607	23.916709288794884
56-57	23.568398283384496	27.327151939232795	25.001702991569914	24.102746785812794
58-59	23.47812686007958	27.255072538217828	25.434218652110257	23.83258194959233
60-61	23.592135210411936	27.38281101678069	25.476919571961755	23.548134200845624
62-63	23.151241030795433	27.43792054052545	25.427478571627997	23.98335985705112
64-65	23.19207843829261	27.33870238189638	25.484068757475885	23.98515042233512
66-67	23.542677784541834	27.265304298775213	25.24465096536741	23.947366951315548
68-69	23.648605630200194	27.136121812306556	25.14668611684179	24.068586440651462
70-71	23.409226975006924	27.205100481632726	25.46744734655217	23.918225196808176
72-73	23.67065956553209	27.259353749095233	25.375981842065286	23.69400484330739
74-75	23.61983954817911	27.164882006921186	25.5111022740728	23.7041761708269
76-77	23.548223151804216	27.00876276856573	25.44721395341044	23.99580012621961
78-79	23.598045764146878	27.058392513677195	25.44181412420165	23.90174759797428
80-81	23.48271609902556	27.195839532483546	25.62840394779757	23.693040420693322
82-83	23.60640321299853	27.17713374352078	25.496139554571723	23.720323488908967
84-85	23.569049889776583	27.22909272838136	25.313304759373455	23.888552622468602
86-87	23.32375286409133	27.33750050247216	25.519667877066276	23.819078756370228
88-89	23.617273557540937	26.835131266621858	25.393286276681504	24.154308899155705
90-91	23.80981093647412	26.920336946666488	25.427547980508546	23.842304136350844
92-93	23.465757982435182	27.13570195633582	25.593811896111667	23.804728165117332
94-95	23.03284105980967	27.45370142948372	25.5923805525908	23.921076958115815
96-97	23.14997142295808	27.369438639732806	25.422181142724465	24.05840879458465
98-99	23.00920917635422	27.381809615992193	25.322053236608514	24.28692797104507
100-101	23.160368406363382	27.755847055540052	25.22478370080938	23.85900083728719
102-103	23.257761146041727	27.63155544441269	25.33994087596456	23.770742533581025
104-105	23.165408027009768	27.74574734613856	25.430959409429306	23.657885217422372
106-107	23.134409172355664	27.811685375697053	25.357053146298846	23.696852305648434
108-109	23.16397427180293	27.845604022012548	25.254920612591008	23.735501093593516
110-111	22.990311161421477	28.246441899900983	25.10524429738338	23.658002641294168
112-113	23.01762016182328	28.40648275492525	24.999665100826977	23.576231982424492
114-115	22.897612022608254	28.52065074086354	25.06901813951879	23.51271909700942
116-117	22.90992674556979	28.33088854207832	25.263847677225037	23.495337035126852
118-119	22.812548140573824	28.188664164659183	25.2990018017133	23.699785893053694
120-121	22.877592304806814	28.170158798206597	25.41787238983263	23.534376507153958
122-123	22.919655226691464	28.413656523889312	25.241550073288327	23.42513817613089
124-125	22.922100834330838	28.25904900577064	25.06308845336144	23.75576170653708
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2254.0
1	1445.5
2	496.5
3	314.5
4	238.5
5	172.0
6	145.5
7	159.5
8	156.5
9	144.5
10	146.5
11	147.0
12	139.5
13	145.0
14	164.0
15	173.5
16	208.0
17	240.0
18	279.0
19	318.0
20	372.5
21	468.0
22	547.0
23	668.0
24	844.5
25	1116.0
26	1466.5
27	1878.5
28	2413.0
29	3209.0
30	4432.5
31	5490.5
32	6532.5
33	7621.5
34	8649.0
35	9659.0
36	10321.5
37	10836.0
38	10750.0
39	10548.0
40	10487.5
41	10354.5
42	10708.5
43	11457.0
44	12370.5
45	13132.0
46	14271.0
47	15521.0
48	16620.5
49	17678.5
50	18388.5
51	18156.5
52	17395.5
53	17118.5
54	16677.0
55	16178.5
56	15478.5
57	14272.0
58	13017.5
59	11786.0
60	10278.5
61	8581.5
62	7004.0
63	5405.5
64	4084.5
65	3334.0
66	2723.0
67	2067.0
68	1620.5
69	1380.5
70	1183.0
71	1021.5
72	915.0
73	902.0
74	733.5
75	613.0
76	517.5
77	353.0
78	250.0
79	156.0
80	115.0
81	107.0
82	91.5
83	50.0
84	34.5
85	24.0
86	12.5
87	5.0
88	0.5
89	0.0
90	1.0
91	1.5
92	1.0
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.331757355922838
2	0.30301544933180635
3	0.2945488411887118
4	0.3085855862680528
5	0.31950305466309586
6	0.3658465939726662
7	0.37609564593535966
8	0.3776552842775087
9	0.3749816185481104
10-11	0.3766526596289843
12-13	0.38712451706912765
14-15	0.373978993899586
16-17	0.30435228219650556
18-19	0.2953286603597863
20-21	0.2765015975152733
22-23	0.2690376140207031
24-25	0.26045960313888356
26-27	0.26714376746237933
28-29	0.251992994995789
30-31	0.25299561964431333
32-33	0.25845435384183485
34-35	0.2513245785634394
36-37	0.24731407996934196
38-39	0.2543324525090125
40-41	0.23717643074537345
42-43	0.24564303888846806
44-45	0.2353939869257746
46-47	0.23528258418704967
48-49	0.24185534577182044
50-51	0.2478710936629666
52-53	0.2474254827080669
54-55	0.24263516494289497
56-57	0.2407413183845712
58-59	0.24341498411396945
60-61	0.24620005258209268
62-63	0.24720267723061704
64-65	0.2523272032119638
66-67	0.2661411428138549
68-69	0.26346747708445667
70-71	0.26246485243593226
72-73	0.26602974007513003
74-75	0.2698174331917776
76-77	0.2650271154266057
78-79	0.26268765791338217
80-81	0.262910463390832
82-83	0.25410964703156264
84-85	0.2439719978075941
86-87	0.2308264746380525
88-89	0.22124583910770862
90-91	0.2308264746380525
92-93	0.22169145006260835
94-95	0.2156757021714622
96-97	0.20275298447937043
98-99	0.21244502274843924
100-101	0.21099678714501516
102-103	0.21166520357736476
104-105	0.2192405898106599
106-107	0.21266782822588912
108-109	0.21979760350428454
110-111	0.2084345241543418
112-113	0.20631787211856817
114-115	0.20776610772199225
116-117	0.20743189950581747
118-119	0.20386701186661974
120-121	0.21344764739696362
122-123	0.20798891319944213
124-125	0.2312720855929522
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	448822.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.04785746482284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.39814995082196	68.21238350050966
2	8.9144418154671	13.915061683840657
3	1.870927321206187	4.3806560667763
4	0.679996350304187	2.1228903290056373
5	0.32467960471844776	1.267027375540022
6	0.1826069816465434	0.8551250205378564
7	0.12496330986306114	0.6827183017567281
8	0.09156569535242827	0.5717205071626971
9	0.06037841758824474	0.42411655168909906
>10	0.3200413175771875	4.497839995288926
>50	0.02327264415514709	1.2481125518257963
>100	0.0083116586106758	1.0573926488639365
>500	3.3246634442703194E-4	0.2366483670586282
>1k	3.3246634442703194E-4	0.5283071001440429
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2036	0.4536319520879102	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	912	0.20319859543427016	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	2.2280547744985764E-4
14-15	0.0	0.0	0.0	0.0	2.2280547744985764E-4
16-17	0.0	0.0	0.0	0.0	2.2280547744985764E-4
18-19	0.0	0.0	0.0	0.0	3.3420821617478647E-4
20-21	0.0	0.0	0.0	6.684164323495728E-4	4.456109548997153E-4
22-23	0.0	0.0	0.0	0.004233304071547294	4.456109548997153E-4
24-25	0.0	0.0	0.0	0.009469232791618949	4.456109548997153E-4
26-27	0.0	0.0	0.0	0.014148147818065959	4.456109548997153E-4
28-29	0.0	0.0	0.0	0.026513851816533054	4.456109548997153E-4
30-31	0.0	0.0	0.0	0.04912860777769361	4.456109548997153E-4
32-33	0.0	0.0	0.0	0.08522309512457055	4.456109548997153E-4
34-35	0.0	0.0	0.0	0.1255508865429948	4.456109548997153E-4
36-37	0.0	0.0	0.0	0.1851513517608317	4.456109548997153E-4
38-39	0.0	0.0	0.0	0.2699288359305025	4.456109548997153E-4
40-41	0.0	0.0	0.0	0.3675176350535402	4.456109548997153E-4
42-43	0.0	0.0	0.0	0.4532977438717354	4.456109548997153E-4
44-45	0.0	0.0	0.0	0.5469874471394005	4.456109548997153E-4
46-47	0.0	0.0	0.0	0.6586129913417791	4.456109548997153E-4
48-49	0.0	0.0	0.0	0.7742490341382553	4.456109548997153E-4
50-51	0.0	0.0	0.0	0.898240282339101	4.456109548997153E-4
52-53	0.0	0.0	0.0	1.0067465498571817	4.456109548997153E-4
54-55	0.0	0.0	0.0	1.109348472222841	4.456109548997153E-4
56-57	0.0	0.0	0.0	1.2340081368560365	4.456109548997153E-4
58-59	0.0	0.0	0.0	1.3718133246587734	4.456109548997153E-4
60-61	0.0	0.0	0.0	1.5076132631644616	4.456109548997153E-4
62-63	0.0	0.0	0.0	1.638734286643703	8.912219097994305E-4
64-65	0.0	0.0	0.0	1.760608882808775	8.912219097994305E-4
66-67	0.0	0.0	0.0	1.8976342514404374	8.912219097994305E-4
68-69	0.0	0.0	0.0	2.064515554050381	8.912219097994305E-4
70-71	0.0	0.0	0.0	2.239640659325969	8.912219097994305E-4
72-73	0.0	0.0	0.0	2.405853545503563	8.912219097994305E-4
74-75	0.0	0.0	0.0	2.5610375605473883	8.912219097994305E-4
76-77	0.0	0.0	0.0	2.7435152465788217	8.912219097994305E-4
78-79	0.0	0.0	0.0	2.9366875955278484	8.912219097994305E-4
80-81	0.0	0.0	0.0	3.1467931607630644	8.912219097994305E-4
82-83	0.0	0.0	0.0	3.360018002682578	0.001114027387249288
84-85	0.0	0.0	0.0	3.5710147898275935	0.001114027387249288
86-87	0.0	0.0	0.0	3.7783352865946855	0.001114027387249288
88-89	0.0	0.0	0.0	4.016959952943483	0.001114027387249288
90-91	0.0	0.0	0.0	4.258258285021679	0.001114027387249288
92-93	0.0	0.0	0.0	4.509805669062569	0.001114027387249288
94-95	0.0	0.0	0.0	4.7504355847084145	0.001114027387249288
96-97	0.0	0.0	0.0	4.990062875705736	0.001114027387249288
98-99	0.0	0.0	0.0	5.260548725329864	0.001114027387249288
100-101	0.0	0.0	0.0	5.573256212930739	0.001114027387249288
102-103	0.0	0.0	0.0	5.874934829397846	0.001114027387249288
104-105	0.0	0.0	0.0	6.199451007303564	0.001114027387249288
106-107	0.0	0.0	0.0	6.51761722910196	0.001114027387249288
108-109	0.0	0.0	0.0	6.859846442464942	0.001114027387249288
110-111	0.0	0.0	0.0	7.200961628440673	0.001114027387249288
112-113	0.0	0.0	0.0	7.5535512965050735	0.001114027387249288
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	55	1.2980578E-5	54.17049	2
ATCACGC	75	8.1100574E-5	39.72502	3
GGTATCA	1780	0.0	32.468227	1
TCACGCA	95	3.254948E-4	31.361862	4
GTATCAA	2410	0.0	30.655718	1
ATCAACG	2920	0.0	24.896164	3
TATCAAC	3000	0.0	24.82814	2
TCAACGC	3005	0.0	24.191946	4
GTACATG	4535	0.0	24.173937	1
AACGCAG	2935	0.0	24.165262	6
CAACGCA	3030	0.0	23.99234	5
CTAGAAC	150	1.6676424E-4	23.835014	3
GTATCAC	175	1.7293716E-5	23.832352	1
ACATGGG	4455	0.0	23.67451	3
TATTCTC	305	2.6921043E-10	23.444279	5
TACATGG	4655	0.0	23.42539	2
CATGGGG	1605	0.0	22.275715	4
CATGGGT	745	0.0	21.595484	4
ACGCAGA	3320	0.0	21.362963	7
CACGCAG	170	3.8896452E-4	21.030897	5
>>END_MODULE