FastQCFastQC Report
Sun 1 Jan 2017
ERR1142654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448822
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31720.7067389744709484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27540.6136062848969079No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16930.37720967332260896No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.15551822326000064No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6840.1523989465757026No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4670.10405015796908351No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22650.044.6472131
GTATCAA36000.042.6315231
GTACTAG1408.063944E-833.9919131
CAACGCA46550.033.5973135
TCAACGC46900.033.346594
ATCAACG46600.033.1784363
AACGCAG47900.032.774566
TATCAAC47850.032.435982
ACGCAGA53050.029.256587
CGCAGAG54450.028.5043478
GCAGAGT56950.027.2530579
GAGTACT25700.026.49359112-13
AGTACTT26950.025.2647612-13
GTACTTT29150.023.66398614-15
AGAGTAC56100.023.00198710-11
CAGAGTA56450.022.64868410-11
CTTAGGC1602.6179416E-422.2997363
GAGTTAG1400.003157052221.2449461
TACTTTT32900.020.8763514-15
ACATGGG41250.020.7590273
Produced by FastQC (version 0.11.5)