##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142654_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448822
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.72310403678964	27.0	14.0	33.0	14.0	33.0
2	29.638161676566657	33.0	27.0	33.0	27.0	33.0
3	29.015028229453993	33.0	27.0	33.0	14.0	33.0
4	30.311453092762832	33.0	27.0	33.0	27.0	33.0
5	31.30825583416143	33.0	33.0	33.0	27.0	33.0
6	33.032398144475984	37.0	33.0	37.0	27.0	37.0
7	35.190371684097485	37.0	37.0	37.0	33.0	37.0
8	34.825140033242576	37.0	37.0	37.0	27.0	37.0
9	35.61269723854891	37.0	37.0	37.0	33.0	37.0
10-11	35.774392743671214	37.0	37.0	37.0	33.0	37.0
12-13	35.856294923154394	37.0	37.0	37.0	33.0	37.0
14-15	35.96632963624778	37.0	37.0	37.0	33.0	37.0
16-17	36.004847133161924	37.0	37.0	37.0	33.0	37.0
18-19	36.05408602965095	37.0	37.0	37.0	37.0	37.0
20-21	36.09140928920597	37.0	37.0	37.0	37.0	37.0
22-23	36.091794742681955	37.0	37.0	37.0	37.0	37.0
24-25	36.082272036575745	37.0	37.0	37.0	37.0	37.0
26-27	35.974475404503345	37.0	37.0	37.0	35.0	37.0
28-29	35.942023786712774	37.0	37.0	37.0	33.0	37.0
30-31	35.94881823974761	37.0	37.0	37.0	35.0	37.0
32-33	35.88647615312975	37.0	37.0	37.0	33.0	37.0
34-35	35.80117953219762	37.0	37.0	37.0	33.0	37.0
36-37	35.711225162759405	37.0	37.0	37.0	33.0	37.0
38-39	35.63714791164426	37.0	37.0	37.0	33.0	37.0
40-41	35.54539661603041	37.0	37.0	37.0	33.0	37.0
42-43	35.449798583848384	37.0	37.0	37.0	33.0	37.0
44-45	35.33251378051878	37.0	37.0	37.0	33.0	37.0
46-47	35.17419155032508	37.0	37.0	37.0	33.0	37.0
48-49	35.00750074639835	37.0	37.0	37.0	30.0	37.0
50-51	34.90412680305332	37.0	37.0	37.0	27.0	37.0
52-53	34.83794577805901	37.0	37.0	37.0	27.0	37.0
54-55	34.776338949516735	37.0	37.0	37.0	27.0	37.0
56-57	34.74141307689908	37.0	37.0	37.0	27.0	37.0
58-59	34.69108466162532	37.0	37.0	37.0	27.0	37.0
60-61	34.651772863184064	37.0	37.0	37.0	27.0	37.0
62-63	34.63011839883072	37.0	37.0	37.0	27.0	37.0
64-65	34.59929660310769	37.0	37.0	37.0	27.0	37.0
66-67	34.5781345388595	37.0	37.0	37.0	27.0	37.0
68-69	34.54164345776277	37.0	37.0	37.0	27.0	37.0
70-71	34.48055688001034	37.0	37.0	37.0	27.0	37.0
72-73	34.45182277161102	37.0	37.0	37.0	27.0	37.0
74-75	34.360258632598224	37.0	37.0	37.0	27.0	37.0
76-77	34.292065674142535	37.0	37.0	37.0	27.0	37.0
78-79	34.2795306379812	37.0	37.0	37.0	27.0	37.0
80-81	34.240793677671775	37.0	37.0	37.0	27.0	37.0
82-83	34.20413660649433	37.0	37.0	37.0	27.0	37.0
84-85	34.08951989875719	37.0	37.0	37.0	27.0	37.0
86-87	33.95951401669259	37.0	37.0	37.0	24.5	37.0
88-89	33.894731096069265	37.0	37.0	37.0	22.0	37.0
90-91	33.86854031219504	37.0	37.0	37.0	22.0	37.0
92-93	33.76124499244689	37.0	37.0	37.0	22.0	37.0
94-95	33.782997491210324	37.0	37.0	37.0	22.0	37.0
96-97	33.77518927325309	37.0	37.0	37.0	22.0	37.0
98-99	33.717445891689806	37.0	35.0	37.0	22.0	37.0
100-101	33.637342866437024	37.0	33.0	37.0	22.0	37.0
102-103	33.5268837089091	37.0	33.0	37.0	18.0	37.0
104-105	33.423290302168795	37.0	33.0	37.0	14.0	37.0
106-107	33.31746327051704	37.0	33.0	37.0	14.0	37.0
108-109	33.21384201309205	37.0	33.0	37.0	14.0	37.0
110-111	33.15358092963358	37.0	33.0	37.0	14.0	37.0
112-113	33.070282873834174	37.0	33.0	37.0	14.0	37.0
114-115	32.96281599386839	37.0	33.0	37.0	14.0	37.0
116-117	32.882197173935324	37.0	33.0	37.0	14.0	37.0
118-119	32.77096710945542	37.0	33.0	37.0	14.0	37.0
120-121	32.67323348677204	37.0	33.0	37.0	14.0	37.0
122-123	32.55027159987701	37.0	33.0	37.0	14.0	37.0
124-125	31.330247848813116	37.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	2.0
17	17.0
18	76.0
19	423.0
20	1533.0
21	3492.0
22	5356.0
23	6888.0
24	5526.0
25	4009.0
26	3927.0
27	4545.0
28	5515.0
29	7250.0
30	9560.0
31	12900.0
32	18058.0
33	26944.0
34	44291.0
35	91772.0
36	196736.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.83229016209276	16.512642878795987	13.151759125519359	25.503307833591897
2	17.369023800081102	20.960648096572807	38.32588420353726	23.344443899808834
3	20.167237791373864	25.70952404293907	29.324320109085562	24.798918056601504
4	13.63641427498758	17.673284864743223	36.110807842018836	32.57949301825036
5	15.210367524833663	35.03919462728338	32.78823314452768	16.962204703355273
6	28.780583450122883	35.241180625338394	19.144721743799757	16.83351418073897
7	25.555565458021217	32.906140964569474	23.0042199357429	18.534073641666406
8	26.062670724697096	31.571536154644825	21.605447148312695	20.760345972345384
9	27.266488719358676	15.756134948821582	21.194593847895156	35.782782483924585
10-11	24.982955380975085	25.464103809528055	27.03053771873928	22.522403090757585
12-13	26.46126972385489	24.650195400403724	27.200761103510967	21.68777377223042
14-15	24.632148156730285	24.603294847400527	26.410915685951224	24.353641309917965
16-17	22.85727972336472	26.90220176372816	27.0775496744812	23.162968838425925
18-19	22.671125746955365	25.067732865144755	30.230247180396685	22.030894207503195
20-21	24.37187861017315	23.878137821858946	30.09768982339186	21.65229374457604
22-23	23.949694979301373	23.87015342385177	30.077179817388632	22.102971779458226
24-25	22.930025711752098	24.640169153918478	29.981930475778817	22.447874658550607
26-27	22.660096875821594	24.614212315795573	30.17343178364697	22.552259024735864
28-29	22.452664976315777	24.61142724732745	30.521565342162372	22.414342434194403
30-31	22.93838091715647	24.351190449666017	30.47544460833025	22.234984024847268
32-33	22.503935306129495	24.4833413729066	30.142495401846837	22.870227919117063
34-35	22.77923715690665	24.493283513917575	30.284790595805454	22.442688733370318
36-37	22.82250499657982	24.552424538832952	29.931239931640995	22.693830532946233
38-39	22.86966490162285	24.393143177992396	29.82742082647722	22.909771093907537
40-41	22.559958644216596	24.70573555249299	29.96489437674985	22.769411426540564
42-43	22.84600550565215	24.42372800162206	29.699216709019264	23.03104978370652
44-45	22.851938705598602	24.635848646721666	29.494505004818215	23.01770764286152
46-47	22.867570223185737	24.690911035406256	29.19016351803288	23.251355223375125
48-49	22.85616569597747	25.038545347598824	28.911572962109698	23.193715994314005
50-51	22.797233647191984	25.21177660631609	28.641198515224296	23.34979123126763
52-53	22.918465358722788	24.972399940733677	28.570266798716194	23.53886790182734
54-55	22.86918082608095	24.99832894402631	28.49629471188767	23.63619551800507
56-57	22.99524754857727	24.95850238736601	28.059515931741164	23.986734132315558
58-59	22.92689502432525	24.924718173584353	28.39575904022495	23.75262776186545
60-61	22.987449339491693	25.019774030181296	28.65485349393188	23.337923136395133
62-63	22.601858422557743	24.923159875362476	28.719769440635083	23.755212261444694
64-65	22.515521203863898	24.8236771188546	28.883531648996314	23.777270028285187
66-67	22.974269166112435	24.793040409340502	28.635565076829327	23.597125347717736
68-69	22.839455285168732	24.68651269322805	28.718846224115573	23.755185797487645
70-71	22.54256245947626	24.777467575438212	29.071479977095038	23.60848998799049
72-73	22.85350792467796	24.98264892679574	28.916969962312244	23.246873186214057
74-75	22.874214236249344	24.817812094712064	29.043679376050335	23.26429429298826
76-77	22.58803087855787	24.653553019268987	29.10991062526626	23.64850547690688
78-79	22.69413473793036	24.983400622086755	28.996328083919327	23.326136556063563
80-81	22.55626952332996	25.11051151681513	29.038460681517392	23.294758278337515
82-83	22.62678093782412	25.070323997277278	28.927031263403336	23.37586380149527
84-85	22.54447056933929	25.18448538170851	28.59523416481884	23.675809884133365
86-87	22.348445095469152	25.288997657650818	28.709408026509408	23.653149220370622
88-89	22.500134047059454	25.038315117827363	28.60944048757384	23.852110347539345
90-91	22.688713909590394	24.88410812040489	28.568226500182327	23.85895146982239
92-93	22.51206122852108	25.2403596662251	28.376111680893874	23.871467424359942
94-95	22.271785388026114	25.161092667275685	28.61188476192598	23.955237182772223
96-97	22.338551826398305	25.092213925166075	28.313546284093526	24.255687964342094
98-99	22.219969408773867	25.166240922059213	28.233830152967272	24.379959516199648
100-101	22.405034523785652	25.454579888195962	27.891854436401147	24.248531151617236
102-103	22.686514289151873	25.360417718035873	27.94731512334023	24.005752869472026
104-105	22.48343832237392	25.387553283702154	27.85069199179102	24.27831640213291
106-107	22.61503271687674	25.453206454466553	27.723388918042357	24.20837191061435
108-109	22.56837822058411	25.49069200497377	27.54471269336875	24.39621708107337
110-111	22.34662204168217	25.85426615000323	27.40358797353697	24.39552383477763
112-113	22.452588022166815	25.79432192682813	27.398452244868853	24.354637806136207
114-115	22.32300027184449	26.008498482572982	27.308895553782868	24.359605691799654
116-117	22.379548171262233	25.9777572548491	27.361718103339754	24.280976470548918
118-119	22.205239323332982	25.784868014281976	27.558751608393166	24.45114105399188
120-121	22.07345005369612	25.935448797073228	27.55257095240429	24.438530196826356
122-123	22.102748973980777	26.064007557561798	27.427577079554922	24.405666388902507
124-125	21.994452118889534	25.994385276948446	27.390156410142154	24.621006194019873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	274.0
1	245.5
2	406.5
3	581.0
4	404.5
5	299.0
6	463.5
7	952.5
8	1575.0
9	1816.0
10	1531.0
11	1141.0
12	969.5
13	917.5
14	917.5
15	895.0
16	895.5
17	896.5
18	885.0
19	882.0
20	901.5
21	923.0
22	952.0
23	1006.0
24	1112.5
25	1236.5
26	1464.0
27	1832.0
28	2279.0
29	2989.0
30	4042.0
31	5059.0
32	6111.5
33	7162.5
34	8230.0
35	9009.0
36	9501.0
37	10254.5
38	10483.5
39	10130.5
40	9774.0
41	9850.5
42	10318.5
43	10885.5
44	11640.0
45	12476.5
46	13625.5
47	14911.0
48	16176.0
49	17157.5
50	18009.5
51	17866.5
52	16992.5
53	17000.5
54	16918.5
55	16248.5
56	15375.0
57	14213.0
58	13177.0
59	11914.5
60	10249.5
61	8519.0
62	6830.5
63	5312.5
64	4091.5
65	3370.0
66	2666.5
67	2005.0
68	1619.5
69	1324.5
70	1075.5
71	967.0
72	891.0
73	870.5
74	724.5
75	605.5
76	506.5
77	372.5
78	267.5
79	145.5
80	101.5
81	91.0
82	70.0
83	50.0
84	31.0
85	20.0
86	16.5
87	5.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04233304071547295
2	0.0
3	0.0
4	0.0020052492970487187
5	0.008020997188194875
6	0.0020052492970487187
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.798191710745017E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	1.1140273872492882E-4
34-35	2.2280547744985764E-4
36-37	0.0033420821617478644
38-39	0.003007873945573078
40-41	0.00746398349457023
42-43	0.002339457513223505
44-45	0.0010026246485243594
46-47	6.684164323495729E-4
48-49	0.0
50-51	0.0
52-53	1.1140273872492882E-4
54-55	8.912219097994305E-4
56-57	2.2280547744985764E-4
58-59	0.0018938465583237898
60-61	2.2280547744985764E-4
62-63	1.1140273872492882E-4
64-65	1.1140273872492882E-4
66-67	5.57013693624644E-4
68-69	0.0
70-71	0.002450860251948434
72-73	5.57013693624644E-4
74-75	0.10081947854606059
76-77	0.10806065656318094
78-79	0.002228054774498576
80-81	0.0
82-83	0.0014482356034240746
84-85	0.12209740164252197
86-87	0.2185721733783103
88-89	0.2713770715339266
90-91	0.10059667306861071
92-93	0.17724175731136174
94-95	0.0026736657293982917
96-97	3.3420821617478647E-4
98-99	1.1140273872492882E-4
100-101	2.2280547744985764E-4
102-103	5.57013693624644E-4
104-105	0.010026246485243593
106-107	0.010583260178868237
108-109	0.015150772466590318
110-111	0.010026246485243593
112-113	0.009580635530343879
114-115	0.008020997188194875
116-117	3.3420821617478647E-4
118-119	0.0010026246485243594
120-121	0.0
122-123	0.0
124-125	4.456109548997153E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	448822.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.32084545562472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.01024107339228	65.53684920945629
2	9.117420771819122	13.73463681816181
3	1.9444141566381745	4.393647545816185
4	0.6938589989496294	2.0904818571151806
5	0.33736675508280883	1.270537461072892
6	0.20334850366782808	0.9189828731038208
7	0.13150561481000697	0.6933579862756014
8	0.09867394660335913	0.5945764066086542
9	0.07083910598095157	0.4802095218425286
>10	0.354758649387349	5.058128649906902
>50	0.025386772760349736	1.3400369535021242
>100	0.010493199393127317	1.593715941308003
>500	6.76980606008214E-4	0.352346093567055
>1k	0.0010154709090123208	1.9424926822629462
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3172	0.7067389744709484	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2754	0.6136062848969079	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1693	0.37720967332260896	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	698	0.15551822326000064	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	684	0.1523989465757026	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	467	0.10405015796908351	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2280547744985764E-4
2	0.0	0.0	0.0	0.0	2.2280547744985764E-4
3	0.0	0.0	0.0	0.0	2.2280547744985764E-4
4	0.0	0.0	0.0	4.456109548997153E-4	2.2280547744985764E-4
5	0.0	0.0	0.0	4.456109548997153E-4	2.2280547744985764E-4
6	0.0	0.0	0.0	4.456109548997153E-4	2.2280547744985764E-4
7	0.0	0.0	0.0	4.456109548997153E-4	2.2280547744985764E-4
8	0.0	0.0	0.0	4.456109548997153E-4	2.2280547744985764E-4
9	0.0	0.0	0.0	4.456109548997153E-4	2.2280547744985764E-4
10-11	0.0	0.0	0.0	4.456109548997153E-4	2.2280547744985764E-4
12-13	0.0	0.0	0.0	4.456109548997153E-4	2.2280547744985764E-4
14-15	0.0	0.0	0.0	4.456109548997153E-4	2.2280547744985764E-4
16-17	0.0	0.0	0.0	4.456109548997153E-4	2.2280547744985764E-4
18-19	0.0	0.0	0.0	4.456109548997153E-4	2.2280547744985764E-4
20-21	0.0	0.0	0.0	0.0011140273872492883	2.2280547744985764E-4
22-23	0.0	0.0	0.0	0.004233304071547294	2.2280547744985764E-4
24-25	0.0	0.0	0.0	0.009246427314169092	2.2280547744985764E-4
26-27	0.0	0.0	0.0	0.014259550556790887	2.2280547744985764E-4
28-29	0.0	0.0	0.0	0.02673665729398292	2.2280547744985764E-4
30-31	0.0	0.0	0.0	0.049017205038968684	2.2280547744985764E-4
32-33	0.0	0.0	0.0	0.0846660814309459	4.456109548997153E-4
34-35	0.0	0.0	0.0	0.12410265093957071	4.456109548997153E-4
36-37	0.0	0.0	0.0	0.18448293532848212	4.456109548997153E-4
38-39	0.0	0.0	0.0	0.26926041949815294	4.456109548997153E-4
40-41	0.0	0.0	0.0	0.3668492186211906	6.684164323495728E-4
42-43	0.0	0.0	0.0	0.45240652196193587	6.684164323495728E-4
44-45	0.0	0.0	0.0	0.5466532389232257	0.0010026246485243594
46-47	0.0	0.0	0.0	0.6583901858643293	0.001114027387249288
48-49	0.0	0.0	0.0	0.7743604368769802	0.0013368328646991457
50-51	0.0	0.0	0.0	0.8983516850778259	0.0015596383421490035
52-53	0.0	0.0	0.0	1.0075263690282563	0.0016710410808739324
54-55	0.0	0.0	0.0	1.111019513303715	0.001782443819598861
56-57	0.0	0.0	0.0	1.2357905806756353	0.001782443819598861
58-59	0.0	0.0	0.0	1.3733729630009224	0.001782443819598861
60-61	0.0	0.0	0.0	1.5087272905517108	0.001782443819598861
62-63	0.0	0.0	0.0	1.6410737441569263	0.001782443819598861
64-65	0.0	0.0	0.0	1.7630597430607233	0.001782443819598861
66-67	0.0	0.0	0.0	1.8996395007374862	0.0018938465583237898
68-69	0.0	0.0	0.0	2.0666322060861546	0.0020052492970487187
70-71	0.0	0.0	0.0	2.241868714100468	0.0020052492970487187
72-73	0.0	0.0	0.0	2.408193003016786	0.0020052492970487187
74-75	0.0	0.0	0.0	2.5639340317542363	0.0020052492970487187
76-77	0.0	0.0	0.0	2.74618891230822	0.0020052492970487187
78-79	0.0	0.0	0.0	2.939584066734697	0.0020052492970487187
80-81	0.0	0.0	0.0	3.150358048402262	0.0020052492970487187
82-83	0.0	0.0	0.0	3.3625802656732513	0.002228054774498576
84-85	0.0	0.0	0.0	3.571571803521218	0.002228054774498576
86-87	0.0	0.0	0.0	3.777889675639786	0.002228054774498576
88-89	0.0	0.0	0.0	4.014731898168985	0.002228054774498576
90-91	0.0	0.0	0.0	4.255250411076106	0.002228054774498576
92-93	0.0	0.0	0.0	4.507243406071895	0.002228054774498576
94-95	0.0	0.0	0.0	4.7499899737535145	0.002228054774498576
96-97	0.0	0.0	0.0	4.990619889399361	0.002228054774498576
98-99	0.0	0.0	0.0	5.261885558194562	0.002228054774498576
100-101	0.0	0.0	0.0	5.572587796498389	0.002228054774498576
102-103	0.0	0.0	0.0	5.873152385578248	0.002228054774498576
104-105	0.0	0.0	0.0	6.198336979916315	0.002228054774498576
106-107	0.0	0.0	0.0	6.515946188021086	0.002228054774498576
108-109	0.0	0.0	0.0	6.859957845203667	0.002228054774498576
110-111	0.0	0.0	0.0	7.205306335250945	0.002228054774498576
112-113	0.0	0.0	0.0	7.560235460828569	0.002228054774498576
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2265	0.0	44.647213	1
GTATCAA	3600	0.0	42.631523	1
GTACTAG	140	8.063944E-8	33.991913	1
CAACGCA	4655	0.0	33.597313	5
TCAACGC	4690	0.0	33.34659	4
ATCAACG	4660	0.0	33.178436	3
AACGCAG	4790	0.0	32.77456	6
TATCAAC	4785	0.0	32.43598	2
ACGCAGA	5305	0.0	29.25658	7
CGCAGAG	5445	0.0	28.504347	8
GCAGAGT	5695	0.0	27.253057	9
GAGTACT	2570	0.0	26.493591	12-13
AGTACTT	2695	0.0	25.26476	12-13
GTACTTT	2915	0.0	23.663986	14-15
AGAGTAC	5610	0.0	23.001987	10-11
CAGAGTA	5645	0.0	22.648684	10-11
CTTAGGC	160	2.6179416E-4	22.299736	3
GAGTTAG	140	0.0031570522	21.244946	1
TACTTTT	3290	0.0	20.87635	14-15
ACATGGG	4125	0.0	20.759027	3
>>END_MODULE