ERR1142653_2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1142653_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	91799
Sequences flagged as poor quality	0
Sequence length	125
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	384	0.4183052102964085	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	347	0.37799976034597327	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	238	0.2592620834649615	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	223	0.242922036187758	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	199	0.2167779605442325	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	182	0.19825924029673528	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	176	0.19172322138585388	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	176	0.19172322138585388	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	174	0.18954454841556007	No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT	171	0.1862765389601194	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	169	0.18409786598982558	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	168	0.18300852950467872	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	164	0.1786511835640911	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	157	0.17102582816806283	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	154	0.16775781871262216	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	146	0.15904312683144697	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	145	0.15795379034630005	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	143	0.15577511737600627	No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	136	0.14814976197997798	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	135	0.14706042549483112	No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA	135	0.14706042549483112	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	131	0.1427030795542435	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	130	0.14161374306909663	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	124	0.13507772415821523	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	123	0.13398838767306834	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	119	0.12963104173248075	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	119	0.12963104173248075	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	111	0.12091634985130557	No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA	111	0.12091634985130557	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	109	0.11873767688101178	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	106	0.11546966742557109	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	105	0.11438033094042419	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	104	0.1132909944552773	No Hit
ACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCT	103	0.11220165797013039	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	103	0.11220165797013039	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	102	0.11111232148498351	No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG	101	0.1100229849998366	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	100	0.1089336485146897	No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA	100	0.1089336485146897	No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG	99	0.1078443120295428	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	98	0.1067549755443959	No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT	95	0.10348696608895522	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	93	0.10130829311866141	No Hit
CTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATA	93	0.10130829311866141	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	93	0.10130829311866141	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	92	0.10021895663351453	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	40	1.5643309E-10	104.18774	1
GGTAACA	25	0.0020458794	71.44302	1
GGTAAAA	25	0.0020458794	71.44302	1
TACAAGA	25	0.0020458794	71.44302	2
CAAAAGA	35	9.080373E-5	68.04098	4
GTACAAG	60	3.1079617E-7	59.535854	1
TTCTCTG	70	1.3171302E-8	59.535854	7
TCTCTGC	75	2.2704626E-8	55.5668	8
GAACAAA	75	2.2704626E-8	55.5668	1
ACAAAAG	55	1.2965051E-5	54.123505	3
GTGTAAG	35	0.0077549466	51.03073	1
GTAACAA	50	5.290971E-4	47.62868	2
TATTCTC	90	9.5475116E-8	46.30566	5
TACAAAA	85	3.4314653E-6	42.02531	2
ACACAGA	60	0.0012983518	39.69057	8
ACGCCAC	30	0.0013052749	39.668854	40-41
GCACGCC	30	0.0013052749	39.668854	38-39
GCCACAG	30	0.0013052749	39.668854	42-43
GAAGGTA	75	0.003880371	31.752457	9
TGGGGGA	240	1.2732926E-11	29.767927	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content