FastQCFastQC Report
Sun 1 Jan 2017
ERR1142651_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142651_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134647
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4570.339406002361731No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3010.2235474982732627No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2610.19384018953262977No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA2540.18864141050301902No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC2460.18269994875489243No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2450.1819572660363766No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1960.14556581282910128No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT1870.13888166836245888No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1870.13888166836245888No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA1870.13888166836245888No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA1810.13442557205136393No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA1790.13294020661433228No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT1680.12477069671065824No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1590.11808655224401583No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT1570.1166011868069842No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1560.11585850408846836No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1510.11214509049588925No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC1470.10917435962182595No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1460.10843167690331014No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG1410.10471826331073103No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTG1410.10471826331073103No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA1400.1039755805922152No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1380.10249021515518356No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCTC1256.0754246E-1042.897345
CATGGGT705.383494E-542.5568854
GTACAAA758.085994E-539.7197571
GAACAAA600.001295834939.7197531
TCTCTGC1401.8426363E-938.3011938
TTCTCTG1401.8426363E-938.3011937
TGGGGGA3050.031.2548896
TCTTGAG800.005310688629.7898182
TATATGA800.005310688629.7898182
ATGGGGG8350.027.1140845
AGTAACG557.7594875E-427.03124118-119
CATGGGG9500.026.9676254
GGTATCA11450.026.5376551
GGGGGGA3150.026.479847
ATGGGGA1159.924013E-425.904195
CTCTGCA2308.023562E-925.904199
GTGATGA951.1642041E-625.0487928-29
CTAGTAC1951.4319812E-624.4429243
ATTCTCT2451.571243E-824.3182186
GTATCAA14650.023.5878411
Produced by FastQC (version 0.11.5)