FastQCFastQC Report
Sun 1 Jan 2017
ERR1142649_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142649_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618788
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16030.2590547974427429No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12360.19974530857094835No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18250.042.1165161
GTATCAA26100.032.8736231
TATCAAC33800.025.2063332
ATCAACG33600.024.6471043
CAACGCA33750.024.5395495
AACGCAG34100.024.291626
TCAACGC34300.024.1441024
GTACATG52600.022.4287471
GGACTAA1602.5847345E-422.343791
TACATGG53950.021.644872
ATGGGTA4201.8189894E-1221.27985
ACATGGG53750.021.0603433
CATGGGT8800.020.9879194
ACGCAGA39250.020.8006537
CGCAGAG40000.020.410648
CATGGGG28950.020.3740314
GCAGAGT42850.019.1921799
ATGGGGG16900.019.0384965
CAGAGTA41800.018.89016210-11
AGAGTAC40450.018.78398510-11
Produced by FastQC (version 0.11.5)