##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142649_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	618788
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.821673982042316	33.0	33.0	33.0	27.0	33.0
2	30.974488839473292	33.0	33.0	33.0	27.0	33.0
3	31.020321984266015	33.0	33.0	33.0	27.0	33.0
4	30.994835064674817	33.0	33.0	33.0	27.0	33.0
5	30.999366503552103	33.0	33.0	33.0	27.0	33.0
6	34.1772303276728	37.0	37.0	37.0	27.0	37.0
7	34.126765548136035	37.0	37.0	37.0	27.0	37.0
8	34.13099155122595	37.0	37.0	37.0	27.0	37.0
9	34.148173526312725	37.0	37.0	37.0	27.0	37.0
10-11	34.16447070725353	37.0	37.0	37.0	27.0	37.0
12-13	34.1216030369044	37.0	37.0	37.0	27.0	37.0
14-15	34.13186422490417	37.0	37.0	37.0	27.0	37.0
16-17	34.141347278874186	37.0	37.0	37.0	27.0	37.0
18-19	34.105968441534095	37.0	37.0	37.0	27.0	37.0
20-21	34.131254969391776	37.0	37.0	37.0	27.0	37.0
22-23	34.1983878161826	37.0	37.0	37.0	27.0	37.0
24-25	34.2037434468671	37.0	37.0	37.0	27.0	37.0
26-27	34.26479989915771	37.0	37.0	37.0	27.0	37.0
28-29	34.28150271175265	37.0	37.0	37.0	27.0	37.0
30-31	34.300209441682775	37.0	37.0	37.0	27.0	37.0
32-33	34.296295338629704	37.0	37.0	37.0	27.0	37.0
34-35	34.30824773589662	37.0	37.0	37.0	27.0	37.0
36-37	34.274714441779736	37.0	37.0	37.0	27.0	37.0
38-39	34.250167262454994	37.0	37.0	37.0	27.0	37.0
40-41	34.20974631052962	37.0	37.0	37.0	27.0	37.0
42-43	34.21890534399503	37.0	37.0	37.0	27.0	37.0
44-45	34.2343306592888	37.0	37.0	37.0	27.0	37.0
46-47	34.206540042793335	37.0	37.0	37.0	27.0	37.0
48-49	34.16830723931298	37.0	37.0	37.0	27.0	37.0
50-51	34.1594900030382	37.0	37.0	37.0	27.0	37.0
52-53	34.127795787895046	37.0	37.0	37.0	27.0	37.0
54-55	34.085693323076725	37.0	37.0	37.0	27.0	37.0
56-57	34.04376700905641	37.0	37.0	37.0	27.0	37.0
58-59	34.00181564606942	37.0	37.0	37.0	27.0	37.0
60-61	33.97633034254058	37.0	37.0	37.0	27.0	37.0
62-63	33.921136156486554	37.0	37.0	37.0	27.0	37.0
64-65	33.89315969281887	37.0	37.0	37.0	27.0	37.0
66-67	33.84132206830126	37.0	37.0	37.0	27.0	37.0
68-69	33.80908000801567	37.0	37.0	37.0	27.0	37.0
70-71	33.76707127481464	37.0	37.0	37.0	27.0	37.0
72-73	33.71488215673219	37.0	33.0	37.0	22.0	37.0
74-75	33.664202440900596	37.0	33.0	37.0	22.0	37.0
76-77	33.58564160908098	37.0	33.0	37.0	22.0	37.0
78-79	33.526903398255946	37.0	33.0	37.0	22.0	37.0
80-81	33.45539829473099	37.0	33.0	37.0	18.0	37.0
82-83	33.413658635914075	37.0	33.0	37.0	14.0	37.0
84-85	33.360260703181055	37.0	33.0	37.0	14.0	37.0
86-87	33.25889642333077	37.0	33.0	37.0	14.0	37.0
88-89	33.18822116783131	37.0	33.0	37.0	14.0	37.0
90-91	33.09090350814819	37.0	33.0	37.0	14.0	37.0
92-93	33.01615577548368	37.0	33.0	37.0	14.0	37.0
94-95	32.946640044732604	37.0	33.0	37.0	14.0	37.0
96-97	32.81411404228912	37.0	33.0	37.0	14.0	37.0
98-99	32.70561727118173	37.0	33.0	37.0	14.0	37.0
100-101	32.62088308111987	37.0	33.0	37.0	14.0	37.0
102-103	32.53844208355689	37.0	33.0	37.0	14.0	37.0
104-105	32.337138082832894	37.0	33.0	37.0	14.0	37.0
106-107	32.23454882770836	37.0	33.0	37.0	14.0	37.0
108-109	32.098330122756096	37.0	33.0	37.0	14.0	37.0
110-111	31.971111269126098	37.0	33.0	37.0	14.0	37.0
112-113	31.863112245227768	37.0	27.0	37.0	14.0	37.0
114-115	31.7190984634479	37.0	27.0	37.0	14.0	37.0
116-117	31.617866700711716	37.0	27.0	37.0	14.0	37.0
118-119	31.468523953276403	37.0	27.0	37.0	14.0	37.0
120-121	31.33252584083725	37.0	27.0	37.0	14.0	37.0
122-123	31.21568533972863	37.0	27.0	37.0	14.0	37.0
124-125	29.57283754694661	35.0	20.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1154.0
1	56.0
2	26.0
3	32.0
4	25.0
5	12.0
6	20.0
7	20.0
8	13.0
9	16.0
10	19.0
11	21.0
12	22.0
13	24.0
14	46.0
15	166.0
16	275.0
17	682.0
18	1759.0
19	3469.0
20	5230.0
21	6502.0
22	7582.0
23	8240.0
24	9350.0
25	10463.0
26	11814.0
27	13586.0
28	15671.0
29	18264.0
30	21456.0
31	25364.0
32	31177.0
33	39569.0
34	56479.0
35	97691.0
36	232493.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.9096313372273	19.195397267735643	12.31934454187478	24.575626853162273
2	17.913461756631044	22.381697897319707	35.695647805102574	24.009192540946675
3	20.254235365422822	26.60898510045927	27.871086723300266	25.26569281081764
4	15.258294718382338	18.908927063529497	32.989891229827435	32.84288698826073
5	16.071859859334147	36.872672926767194	30.632834243495545	16.422632970403114
6	30.429600464432934	34.606902277732196	18.3123441214531	16.65115313638177
7	27.09836140014012	32.53587275227691	20.974532291963985	19.391233555618985
8	26.203759264364834	33.19164463761535	20.191044582475147	20.413551515544672
9	26.980705825877255	17.933510754754618	19.43218658116706	35.65359683820107
10-11	25.779569303923978	26.631328083631946	25.117234411249314	22.471868201194763
12-13	26.54444483190105	25.413486030882815	24.854575371437978	23.187493765778157
14-15	24.947414854229272	25.72163686166012	23.14897854266573	26.181969741444878
16-17	24.602759753020987	27.539748336846436	23.892682934735657	23.964808975396917
18-19	24.684035112881936	26.745818939985107	25.141056838430664	23.429089108702293
20-21	26.612735963704125	25.300980312727862	24.630640849064246	23.455642874503766
22-23	26.410699703164088	25.139182340207654	24.377244092446887	24.072873864181368
24-25	25.327487923996717	25.82267898933391	24.38651959176102	24.463313494908352
26-27	24.897601742066566	25.9512344498848	24.586032359145687	24.565131448902946
28-29	24.7618600190512	25.904364927650807	24.8655400107568	24.4682350425412
30-31	25.179256470405264	25.644853484363455	24.895831983307197	24.280058061924077
32-33	24.84266463609742	25.85443896748794	24.563353355502183	24.73954304091246
34-35	25.00259204178371	25.866713971429217	24.775950388320258	24.354743598466808
36-37	24.942047227257486	26.1087163238098	24.494381294416286	24.454855154516427
38-39	24.876516910176885	25.910875097806386	24.51680001814379	24.695807973872938
40-41	24.604675459305362	25.94033585882105	24.779205422982074	24.675783258891514
42-43	24.797759397668038	25.944788396556806	24.67643123495547	24.581020970819687
44-45	24.833654578143474	26.00787184761658	24.515703202189865	24.642770372050084
46-47	24.644993464348712	25.935521386238364	24.624017830098982	24.795467319313943
48-49	24.671944070533847	26.15449283954457	24.60221041906604	24.571352670855546
50-51	24.469036368437166	26.327307834983742	24.67808482134902	24.525570975230064
52-53	24.59087695085178	25.95611884472803	24.62092587957912	24.83207832484107
54-55	24.38851939081953	25.761070633704485	25.00882799819876	24.84158197727722
56-57	24.55439434237935	25.697159851108424	24.538520349487012	25.20992545702521
58-59	24.589271699858344	25.893478318972186	24.735623395861495	24.781626585307976
60-61	24.689817158361514	25.80873509223075	24.940895377326015	24.560552372081723
62-63	24.30577210453207	25.880961733939717	24.963208047657425	24.850058113870787
64-65	24.31273697503019	25.463744086002343	25.123139940918747	25.10037899804872
66-67	24.504427725313334	25.578974400934555	24.89482582768205	25.021772046070055
68-69	24.542676752539155	25.5945485740393	24.686058587275795	25.17671608614575
70-71	24.40595531167044	25.603563012521253	24.86534609031796	25.125135585490348
72-73	24.564005767890993	25.810664117561284	24.80833103805836	24.816999076489363
74-75	24.516484451267676	25.61479975209524	25.048021420267432	24.820694376369655
76-77	24.340580514347604	25.311371720521652	25.025902076783556	25.322145688347184
78-79	24.487053305629473	25.488847576114736	24.89037938897597	25.133719729279818
80-81	24.41175779658367	25.670098332662338	25.07689453803738	24.84124933271661
82-83	24.4168669370418	25.689039439291548	25.117185506661777	24.77690811700488
84-85	24.272892055146006	25.799757995150184	24.86235366724819	25.06499628245562
86-87	23.987625977349385	25.923577453040664	24.996497536128015	25.092299033481936
88-89	24.129950685463953	25.496505874826912	25.054294575401038	25.319248864308097
90-91	24.60150030085381	25.441785395268457	24.81205747563003	25.1446568282477
92-93	24.486421310651032	25.643992734710867	24.889364675892146	24.98022127874595
94-95	23.998571672640974	25.752078321569606	25.049655428170276	25.199694577619148
96-97	24.078189630255732	25.616786935362555	24.98601385579577	25.319009578585938
98-99	23.86607095143323	25.728598703525872	25.016396203529922	25.388934141510976
100-101	24.080415144112624	26.00334725729753	24.75035241745011	25.165885181139735
102-103	24.218681050864777	25.813989453135438	25.13855613375555	24.828773362244235
104-105	24.16125479780394	25.868220318396034	24.96129366608904	25.00923121771098
106-107	24.19455136976873	25.791602767876952	25.080524620534305	24.93332124182001
108-109	24.169249119590717	25.799453737316153	24.96329772287979	25.06799942021334
110-111	23.86170880310912	26.275003542295	24.8448879622695	25.018399692326376
112-113	23.846370583158315	26.45012314772383	24.74103353407249	24.962472735045367
114-115	23.863469832733784	26.36375928368379	24.82396119495068	24.948809688631744
116-117	23.769017829455482	26.42185879421031	24.824486616775605	24.984636759558608
118-119	23.6899355604476	26.021547860880517	25.080013051458533	25.208503527213345
120-121	23.728249516206084	26.147785883743712	25.11485550958276	25.00910909046744
122-123	23.79004606071205	26.34269526009445	25.107257740005135	24.760000939188362
124-125	23.75733604788363	26.292508991138032	24.730832740132826	25.219322220845513
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2084.0
1	1264.0
2	346.0
3	235.5
4	189.5
5	155.0
6	142.0
7	126.0
8	125.5
9	136.5
10	132.5
11	115.0
12	116.5
13	112.0
14	112.5
15	139.0
16	158.0
17	165.5
18	179.5
19	216.5
20	274.5
21	337.0
22	436.5
23	568.5
24	674.5
25	819.0
26	1131.5
27	1493.5
28	1867.0
29	2469.0
30	3199.0
31	3975.5
32	4929.0
33	5861.0
34	6675.0
35	7660.5
36	8769.5
37	10048.5
38	11322.0
39	12120.0
40	12802.0
41	13795.0
42	14905.0
43	16141.0
44	17846.0
45	19611.0
46	21495.5
47	23263.0
48	24816.5
49	26418.5
50	27261.0
51	27076.5
52	26859.5
53	27107.0
54	27137.0
55	26552.0
56	25352.5
57	23335.0
58	21146.0
59	18803.5
60	16146.5
61	13586.5
62	11313.5
63	8991.0
64	6885.0
65	5529.0
66	4464.0
67	3484.0
68	2809.0
69	2458.5
70	2061.0
71	1772.5
72	1550.0
73	1392.5
74	1170.5
75	955.0
76	743.5
77	566.5
78	442.5
79	288.5
80	193.5
81	146.5
82	110.5
83	71.5
84	40.0
85	19.5
86	14.0
87	9.0
88	4.5
89	1.0
90	0.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	1.0
97	1.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3130312805031772
2	0.28539661402612854
3	0.27828593961098147
4	0.2907296198374888
5	0.3013956314602093
6	0.3429284342941363
7	0.3497158962358675
8	0.35197838354977795
9	0.35181677731307004
10-11	0.35254400537825553
12-13	0.3606243172136499
14-15	0.35052392741940697
16-17	0.29291130403304527
18-19	0.2827301111204483
20-21	0.264711015727519
22-23	0.2605092535731139
24-25	0.2507320762522867
26-27	0.2569539163655404
28-29	0.2430557800086621
30-31	0.24499505484915673
32-33	0.2513785011991183
34-35	0.24467184237574094
36-37	0.23836919914413338
38-39	0.24192453635170688
40-41	0.2287536280600141
42-43	0.23497546817326775
44-45	0.22592551891762605
46-47	0.22705676257458127
48-49	0.2325513746226494
50-51	0.2370763492504703
52-53	0.2361875149485769
54-55	0.23174334343911002
56-57	0.2304504935454469
58-59	0.23263217774100337
60-61	0.23440984634479015
62-63	0.23683393989540844
64-65	0.24281337065360029
66-67	0.25816596314084955
68-69	0.25129769808076435
70-71	0.2517017136725341
72-73	0.2550146415250457
74-75	0.26107487540159147
76-77	0.2520249261459498
78-79	0.2497624388320394
80-81	0.24992404506874727
82-83	0.24200533947006084
84-85	0.23352101204289674
86-87	0.22067331622461975
88-89	0.21380505116453452
90-91	0.22277419730182224
92-93	0.2151787041765516
94-95	0.2072599985778651
96-97	0.19594756200831304
98-99	0.20475510190889287
100-101	0.2048359050272468
102-103	0.20338144889687584
104-105	0.21299701998099513
106-107	0.20604795180255595
108-109	0.21323942933605694
110-111	0.20208859900321274
112-113	0.19990691480765627
114-115	0.200310930399426
116-117	0.20079574910954961
118-119	0.19917968674247077
120-121	0.2057247393291402
122-123	0.19934129297917866
124-125	0.22212777235499073
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	618788.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.51155154279817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.32275756749371	73.70159314975123
2	7.575869760821375	12.50193536503078
3	1.6423725040891013	4.065441105708671
4	0.6017369307011667	1.9860099109101799
5	0.27460649720981833	1.1329104074257594
6	0.16394507699366823	0.8116417602330644
7	0.1080597241409924	0.6241322848712033
8	0.06521185353766236	0.4304584971499387
9	0.04705198706451703	0.3494099210278473
>10	0.18337322309858367	2.6289702821351577
>50	0.010009920725647988	0.6061228057108322
>100	0.004549958294435729	0.628459974081049
>500	0.0	0.0
>1k	4.549958294405341E-4	0.5329145359643367
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1603	0.2590547974427429	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1236	0.19974530857094835	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	8.080311835394351E-5	0.0
12-13	0.0	0.0	0.0	2.4240935506183054E-4	0.0
14-15	0.0	0.0	0.0	4.0401559176971757E-4	0.0
16-17	0.0	0.0	0.0	4.848187101236611E-4	0.0
18-19	0.0	0.0	0.0	4.848187101236611E-4	0.0
20-21	0.0	0.0	0.0	4.848187101236611E-4	0.0
22-23	0.0	0.0	0.0	0.0010504405386012657	0.0
24-25	0.0	0.0	0.0	0.003636140325927458	0.0
26-27	0.0	0.0	0.0	0.006464249468315481	0.0
28-29	0.0	0.0	0.0	0.012847695818277018	0.0
30-31	0.0	0.0	0.0	0.02852350077894206	0.0
32-33	0.0	0.0	0.0	0.05357246746866455	0.0
34-35	0.0	0.0	0.0	0.08403524308810126	0.0
36-37	0.0	0.0	0.0	0.12968900495807933	0.0
38-39	0.0	0.0	0.0	0.19950289921588654	0.0
40-41	0.0	0.0	0.0	0.2740841774565764	0.0
42-43	0.0	0.0	0.0	0.3372722160093602	0.0
44-45	0.0	0.0	0.0	0.4092677944627239	0.0
46-47	0.0	0.0	0.0	0.49782801217864603	0.0
48-49	0.0	0.0	0.0	0.5869538517230457	8.080311835394351E-5
50-51	0.0	0.0	0.0	0.6706658823377312	1.6160623670788703E-4
52-53	0.0	0.0	0.0	0.7513881975733208	1.6160623670788703E-4
54-55	0.0	0.0	0.0	0.832352922163972	1.6160623670788703E-4
56-57	0.0	8.080311835394351E-5	0.0	0.9272157831115018	1.6160623670788703E-4
58-59	0.0	1.6160623670788703E-4	0.0	1.024341131372942	1.6160623670788703E-4
60-61	0.0	1.6160623670788703E-4	0.0	1.1255066355520793	1.6160623670788703E-4
62-63	0.0	1.6160623670788703E-4	0.0	1.223116802523643	1.6160623670788703E-4
64-65	0.0	1.6160623670788703E-4	0.0	1.3209693788502685	1.6160623670788703E-4
66-67	0.0	1.6160623670788703E-4	0.0	1.4307808166932778	1.6160623670788703E-4
68-69	0.0	1.6160623670788703E-4	0.0	1.5482685507799117	1.6160623670788703E-4
70-71	0.0	1.6160623670788703E-4	0.0	1.6774727370278675	3.2321247341577405E-4
72-73	0.0	1.6160623670788703E-4	0.0	1.803929617251789	4.848187101236611E-4
74-75	0.0	1.6160623670788703E-4	0.0	1.9266695540314291	5.656218284776045E-4
76-77	0.0	1.6160623670788703E-4	0.0	2.0574089995281097	6.464249468315481E-4
78-79	0.0	1.6160623670788703E-4	0.0	2.2056827217075963	7.272280651854917E-4
80-81	0.0	1.6160623670788703E-4	0.0	2.365107274219927	8.080311835394351E-4
82-83	0.0	1.6160623670788703E-4	0.0	2.525259054797443	9.696374202473222E-4
84-85	0.0	1.6160623670788703E-4	0.0	2.6799970264452444	9.696374202473222E-4
86-87	0.0	1.6160623670788703E-4	0.0	2.8463706471360144	9.696374202473222E-4
88-89	0.0	1.6160623670788703E-4	0.0	3.023329476331151	9.696374202473222E-4
90-91	0.0	1.6160623670788703E-4	0.0	3.202874005313613	9.696374202473222E-4
92-93	0.0	1.6160623670788703E-4	0.0	3.3927613334453803	9.696374202473222E-4
94-95	0.0	1.6160623670788703E-4	0.0	3.5842647239442265	9.696374202473222E-4
96-97	0.0	1.6160623670788703E-4	0.0	3.783040395094927	9.696374202473222E-4
98-99	0.0	1.6160623670788703E-4	0.0	3.994259746472136	9.696374202473222E-4
100-101	0.0	1.6160623670788703E-4	0.0	4.2279423647517405	9.696374202473222E-4
102-103	0.0	1.6160623670788703E-4	0.0	4.469947704221801	9.696374202473222E-4
104-105	0.0	1.6160623670788703E-4	0.0	4.720437371119026	9.696374202473222E-4
106-107	0.0	1.6160623670788703E-4	0.0	4.983855536952882	9.696374202473222E-4
108-109	0.0	1.6160623670788703E-4	0.0	5.252121889887975	0.0010504405386012657
110-111	0.0	1.6160623670788703E-4	0.0	5.5250748236875955	0.0011312436569552093
112-113	0.0	1.6160623670788703E-4	0.0	5.821541464928215	0.0011312436569552093
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1825	0.0	42.116516	1
GTATCAA	2610	0.0	32.873623	1
TATCAAC	3380	0.0	25.206333	2
ATCAACG	3360	0.0	24.647104	3
CAACGCA	3375	0.0	24.539549	5
AACGCAG	3410	0.0	24.29162	6
TCAACGC	3430	0.0	24.144102	4
GTACATG	5260	0.0	22.428747	1
GGACTAA	160	2.5847345E-4	22.34379	1
TACATGG	5395	0.0	21.64487	2
ATGGGTA	420	1.8189894E-12	21.2798	5
ACATGGG	5375	0.0	21.060343	3
CATGGGT	880	0.0	20.987919	4
ACGCAGA	3925	0.0	20.800653	7
CGCAGAG	4000	0.0	20.41064	8
CATGGGG	2895	0.0	20.374031	4
GCAGAGT	4285	0.0	19.192179	9
ATGGGGG	1690	0.0	19.038496	5
CAGAGTA	4180	0.0	18.890162	10-11
AGAGTAC	4045	0.0	18.783985	10-11
>>END_MODULE