FastQCFastQC Report
Sun 1 Jan 2017
ERR1142649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618788
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28070.45362870643903885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27740.44829570062767865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10890.17598919177488898No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8510.13752690743841187No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25000.056.874351
GTATCGC350.007798706650.993141
GTCAACG2500.049.9732781
GCGTACA1056.384653E-945.3272361
GCGCAGA4400.039.2106361
GTATCAA37350.037.9000322
ATCAACG44350.031.9180684
TATCAAC45450.031.40733
CAACGCA45950.031.0630366
TCAACGC46350.031.0540964
AACGCAG48450.029.5829477
GTAAGTA2900.028.713312
ACGCAGA52700.027.1972247
CGCAGAG56000.025.4883278
GAGTACT23300.025.39716712-13
CAGAGTA58250.024.24855410-11
CATGGGG28050.024.1727034
GCAGAGT59550.023.8690039
CGTACAT1751.7541688E-523.7910292
GTCTAAT1300.002052699722.8815381
Produced by FastQC (version 0.11.5)