FastQCFastQC Report
Sun 1 Jan 2017
ERR1142643_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142643_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290533
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21710.7472473006508727No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5610.19309338353990768No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12950.041.398651
GTATCAA19200.036.2992861
ATCAACG23550.029.346453
AACGCAG23950.029.1050826
TATCAAC23900.028.6674082
CAACGCA23900.028.6674085
TCAACGC23900.028.6674084
GTATTGC1159.963239E-425.8991581
ACGCAGA27350.025.0512287
CGCAGAG27600.024.608458
CAATACT1250.001618142423.831344
GCAGAGT28350.023.747289
GTATTTA1350.002533832322.0622461
GTACATG36100.021.1210861
CAGAGTA28300.021.0524210-11
GTACAAG2004.849959E-520.8488221
TACATGG36700.020.779372
ATGGGAG6700.020.452275
TACAAGG2055.8577083E-520.3438282
ACATGGG36350.020.323843
Produced by FastQC (version 0.11.5)