##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142643_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	290533
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.83230476400271	33.0	33.0	33.0	27.0	33.0
2	30.968038054196942	33.0	33.0	33.0	27.0	33.0
3	31.019546832889894	33.0	33.0	33.0	27.0	33.0
4	30.932912956531617	33.0	33.0	33.0	27.0	33.0
5	31.052489734384736	33.0	33.0	33.0	27.0	33.0
6	34.2400656724021	37.0	37.0	37.0	27.0	37.0
7	34.25149638767369	37.0	37.0	37.0	27.0	37.0
8	34.20164318683248	37.0	37.0	37.0	27.0	37.0
9	34.03086396381823	37.0	37.0	37.0	27.0	37.0
10-11	34.16991873556532	37.0	37.0	37.0	27.0	37.0
12-13	34.111911555658054	37.0	37.0	37.0	27.0	37.0
14-15	34.10963470586818	37.0	37.0	37.0	27.0	37.0
16-17	34.19006102576988	37.0	37.0	37.0	27.0	37.0
18-19	34.140269780024994	37.0	37.0	37.0	27.0	37.0
20-21	34.14718465716459	37.0	37.0	37.0	27.0	37.0
22-23	34.26232992465572	37.0	37.0	37.0	27.0	37.0
24-25	34.32844633828171	37.0	37.0	37.0	27.0	37.0
26-27	34.424036168008456	37.0	37.0	37.0	27.0	37.0
28-29	34.469810658341736	37.0	37.0	37.0	27.0	37.0
30-31	34.519090774541965	37.0	37.0	37.0	27.0	37.0
32-33	34.52206289819057	37.0	37.0	37.0	27.0	37.0
34-35	34.54192983241146	37.0	37.0	37.0	27.0	37.0
36-37	34.53263312601322	37.0	37.0	37.0	27.0	37.0
38-39	34.49980208788675	37.0	37.0	37.0	27.0	37.0
40-41	34.455110090764215	37.0	37.0	37.0	27.0	37.0
42-43	34.455607452509696	37.0	37.0	37.0	27.0	37.0
44-45	34.467303197915555	37.0	37.0	37.0	27.0	37.0
46-47	34.46003552092189	37.0	37.0	37.0	27.0	37.0
48-49	34.438251420664784	37.0	37.0	37.0	27.0	37.0
50-51	34.42904248398633	37.0	37.0	37.0	27.0	37.0
52-53	34.4027700811956	37.0	37.0	37.0	27.0	37.0
54-55	34.38177762939149	37.0	37.0	37.0	27.0	37.0
56-57	34.33419955736525	37.0	37.0	37.0	27.0	37.0
58-59	34.30385532796619	37.0	37.0	37.0	27.0	37.0
60-61	34.28163926300971	37.0	37.0	37.0	27.0	37.0
62-63	34.237164108724315	37.0	37.0	37.0	27.0	37.0
64-65	34.217297174503415	37.0	37.0	37.0	27.0	37.0
66-67	34.16818571384317	37.0	37.0	37.0	27.0	37.0
68-69	34.14683874121012	37.0	37.0	37.0	27.0	37.0
70-71	34.0990008019743	37.0	37.0	37.0	27.0	37.0
72-73	34.04987901546468	37.0	37.0	37.0	27.0	37.0
74-75	34.00910912013437	37.0	37.0	37.0	27.0	37.0
76-77	33.950368804920615	37.0	37.0	37.0	27.0	37.0
78-79	33.88987481628593	37.0	37.0	37.0	27.0	37.0
80-81	33.83664506269511	37.0	37.0	37.0	27.0	37.0
82-83	33.78019192312061	37.0	37.0	37.0	22.0	37.0
84-85	33.72702928755081	37.0	35.0	37.0	22.0	37.0
86-87	33.65262121686693	37.0	33.0	37.0	22.0	37.0
88-89	33.570924817490614	37.0	33.0	37.0	22.0	37.0
90-91	33.476916563695	37.0	33.0	37.0	22.0	37.0
92-93	33.41285843604685	37.0	33.0	37.0	14.0	37.0
94-95	33.34985182406129	37.0	33.0	37.0	14.0	37.0
96-97	33.24240964021299	37.0	33.0	37.0	14.0	37.0
98-99	33.108934269084756	37.0	33.0	37.0	14.0	37.0
100-101	33.028537205756315	37.0	33.0	37.0	14.0	37.0
102-103	32.94513704123112	37.0	33.0	37.0	14.0	37.0
104-105	32.75394361397845	37.0	33.0	37.0	14.0	37.0
106-107	32.637359611472704	37.0	33.0	37.0	14.0	37.0
108-109	32.520594906602696	37.0	33.0	37.0	14.0	37.0
110-111	32.4163640619138	37.0	33.0	37.0	14.0	37.0
112-113	32.306250925024	37.0	33.0	37.0	14.0	37.0
114-115	32.17533464356889	37.0	33.0	37.0	14.0	37.0
116-117	32.06218914890907	37.0	33.0	37.0	14.0	37.0
118-119	31.910373348294343	37.0	33.0	37.0	14.0	37.0
120-121	31.768515108438628	37.0	27.0	37.0	14.0	37.0
122-123	31.6405021116362	37.0	27.0	37.0	14.0	37.0
124-125	30.052475966585554	35.0	24.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	525.0
1	27.0
2	14.0
3	10.0
4	8.0
5	11.0
6	9.0
7	10.0
8	11.0
9	12.0
10	8.0
11	11.0
12	8.0
13	11.0
14	24.0
15	89.0
16	168.0
17	319.0
18	847.0
19	1635.0
20	2479.0
21	3126.0
22	3298.0
23	3394.0
24	3719.0
25	4188.0
26	4782.0
27	5484.0
28	6576.0
29	7505.0
30	9042.0
31	10871.0
32	13277.0
33	17220.0
34	24896.0
35	44392.0
36	122527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.31091209766236	19.99164511774515	12.399059557881726	23.298383226710765
2	17.621720131712472	23.940190941661317	35.46572231311395	22.972366613512264
3	19.632577274045033	29.173626737716912	27.821762359015423	23.372033629222635
4	14.315990763208852	20.72720745846723	32.61042901374128	32.34637276458264
5	14.82171891343296	39.832506567523815	29.530210609528346	15.815563909514886
6	28.534868621004062	36.41933523056945	18.1955074185616	16.85028872986489
7	25.76286640901037	35.77761429612514	20.052636487032565	18.406882807831924
8	26.06023971538116	34.66477841870747	19.39380332285586	19.88117854305551
9	27.195924358863653	19.57585700716691	18.533114584232795	34.695104049736635
10-11	25.07590280296358	29.024230178056403	23.973023850232288	21.926843168747734
12-13	25.977412897687923	28.06548498021599	24.02025550817526	21.936846613920828
14-15	24.78430112874926	27.73811497492436	22.789647835758004	24.68793606056838
16-17	23.6841605671869	29.826499212144537	23.172615421256737	23.316724799411826
18-19	24.03091812116673	28.50883399543718	25.07394739294454	22.386300490451553
20-21	26.835118039043813	26.23224065402016	24.404628386655947	22.528012920280077
22-23	26.44156113047379	26.49280513475275	23.909727733876256	23.1559060008972
24-25	24.89562343003837	27.177933585447317	24.155498661219532	23.770944323294778
26-27	24.399409243680083	27.124583330457767	24.430465358629114	24.04554206723304
28-29	24.13877574960883	27.311275917808263	24.714621620269497	23.835326712313407
30-31	24.897010012627916	26.784297218407776	24.727775209257715	23.59091755970659
32-33	24.32026775239804	27.063349665309506	24.4893382099234	24.127044372369056
34-35	24.421136163679336	27.334172891472736	24.713026377076613	23.53166456777132
36-37	24.395292753863192	27.440650541140105	24.3687273806197	23.795329324377008
38-39	24.396033591642112	27.16261722227206	24.374124498851067	24.067224687234763
40-41	24.10101351584438	27.314217509193394	24.482893039236657	24.101875935725573
42-43	24.152861056103333	27.308889180219282	24.394703613493505	24.143546150183884
44-45	24.34225731563025	27.26609473150438	24.4836888093193	23.90795914354607
46-47	24.043720559598324	27.10181398581859	24.660858646401266	24.193606808181823
48-49	23.76222765386015	27.639370673640016	24.69076174984605	23.907639922653782
50-51	23.857963464490865	27.728001932000485	24.533128633282157	23.880905970226493
52-53	23.82407126297196	27.260260267167148	24.584276330315742	24.33139213954515
54-55	23.457776106504568	27.263679189141172	24.676272271105045	24.602272433249215
56-57	23.936104154161406	26.79541048303079	24.2572737496464	25.0112116131614
58-59	23.937571261253023	27.056075814211024	24.64134351503192	24.36500940950403
60-61	24.183304181027196	26.87842625846538	24.919701783983136	24.018567776524286
62-63	23.660736618578824	26.980367533029842	24.86066140765185	24.498234440739484
64-65	23.36502284853723	26.550017509449063	25.21515921523167	24.869800426782042
66-67	23.952512159226778	26.564653960224362	24.683187227075177	24.799646653473683
68-69	23.786782973554903	26.611929277314633	24.64148677745266	24.9598009716778
70-71	23.617878043394246	26.676972340308065	24.85507646441782	24.850073151879865
72-73	23.88770418831908	26.90955328737155	24.741896303044104	24.46084622126526
74-75	23.95442969833744	26.478464012367404	25.039683361284222	24.52742292801093
76-77	23.67712772381429	26.17794724038578	25.069098186711752	25.07582684908818
78-79	23.804980720625913	26.422144970541805	25.079230895304804	24.69364341352748
80-81	23.79494168794424	26.501794217100272	25.137154095645574	24.566109999309916
82-83	23.694665495903767	26.430662487342133	25.263119761109166	24.611552255644934
84-85	23.60257768057046	26.801564823215685	24.893142859114164	24.702714637099696
86-87	23.283994991428166	26.916071927506785	24.98318403052055	24.81674905054449
88-89	23.543330171596104	26.411140812279037	25.12460118996292	24.920927826161936
90-91	23.89330316258229	26.194742769626657	25.09300444455176	24.8189496232393
92-93	23.785716256674164	26.49866861660297	24.9634386942785	24.752176432444365
94-95	23.14474866395002	26.755834386710376	25.178614232920204	24.920802716419406
96-97	23.10920602516001	26.754062293092034	24.977584970205253	25.159146711542707
98-99	22.982930198150413	26.838163655582818	24.834932136372178	25.343974009894588
100-101	23.281009934145974	27.3357141255934	24.652063477830524	24.731212462430097
102-103	23.41241854522066	27.24327195162116	24.935551788771956	24.40875771438622
104-105	23.436691615217466	27.025488911116476	24.97723588452385	24.560583589142208
106-107	23.422941818576877	27.061026775629298	25.01064851619178	24.50538288960205
108-109	23.24447188430191	27.25703366709551	24.785549465900036	24.712944982702542
110-111	22.953867058910767	27.986880745493703	24.588255784077212	24.47099641151832
112-113	22.852733495995242	28.194539043168632	24.389285006078268	24.563442454757862
114-115	22.964150845791735	28.199610297794564	24.48243753556464	24.353801320849065
116-117	22.875303060087806	27.982576847071485	24.61020351153094	24.53191658130977
118-119	22.779770321067698	27.740973204124565	24.87671138393627	24.60254509087147
120-121	22.88712842603354	27.714356662723443	25.04095547837723	24.35755943286578
122-123	22.87248993386962	28.10429114611624	24.72138158178354	24.3018373382306
124-125	22.6607196140625	28.05842875696597	24.478296190126304	24.80255543884522
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1980.0
1	1266.0
2	448.5
3	286.0
4	193.5
5	162.0
6	158.5
7	146.5
8	141.5
9	123.0
10	102.5
11	112.0
12	117.0
13	115.5
14	109.0
15	111.5
16	135.5
17	155.0
18	171.0
19	183.0
20	208.0
21	251.0
22	293.0
23	369.0
24	485.0
25	600.0
26	747.0
27	939.5
28	1156.5
29	1420.5
30	1849.0
31	2326.5
32	2785.0
33	3348.5
34	3809.5
35	4265.0
36	4837.5
37	5460.5
38	5927.5
39	6033.0
40	6203.0
41	6514.5
42	6716.0
43	7168.5
44	8002.5
45	8709.0
46	9557.0
47	10540.0
48	11505.5
49	12424.0
50	13009.5
51	12959.5
52	12536.0
53	12619.5
54	12456.5
55	11854.0
56	11427.5
57	10530.5
58	9274.0
59	8060.5
60	6989.0
61	5844.5
62	4721.0
63	3713.0
64	2765.5
65	2192.5
66	1737.0
67	1359.5
68	1112.5
69	894.5
70	761.0
71	691.0
72	627.5
73	607.5
74	483.5
75	377.5
76	322.0
77	249.0
78	186.0
79	124.0
80	88.0
81	79.0
82	70.0
83	54.5
84	39.0
85	20.0
86	10.5
87	5.5
88	1.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3035799719825287
2	0.2791421284329147
3	0.27019305896404194
4	0.28292827320820696
5	0.29256573263622376
6	0.34040883479673567
7	0.344194979572028
8	0.3521114641021846
9	0.3469485394086042
10-11	0.3503904892043245
12-13	0.3550371214285468
14-15	0.3472927343881762
16-17	0.283100370697993
18-19	0.27535598365762237
20-21	0.26021140455645314
22-23	0.25573686982201677
24-25	0.24678780035314404
26-27	0.25298330998544055
28-29	0.24042019323106154
30-31	0.24059229072084753
32-33	0.24541102043485596
34-35	0.24024809574127554
36-37	0.23474097606812305
38-39	0.24059229072084753
40-41	0.22407093170139022
42-43	0.23233161121111887
44-45	0.2202847869260979
46-47	0.22252205429331606
48-49	0.22975014886432868
50-51	0.23336419614983497
52-53	0.23302000117026295
54-55	0.22975014886432868
56-57	0.2254477116196783
58-59	0.22940595388475662
60-61	0.23267580619069092
62-63	0.23456887857833705
64-65	0.2373224384149133
66-67	0.25212282253651047
68-69	0.24954136018972028
70-71	0.24954136018972028
72-73	0.25022975014886434
74-75	0.25436008990372866
76-77	0.25057394512843634
78-79	0.24627150788378602
80-81	0.24541102043485596
82-83	0.23869921833320137
84-85	0.22716868651753847
86-87	0.21684283713037764
88-89	0.209270547579793
90-91	0.21598234968144753
92-93	0.20961474255936502
94-95	0.20393552539642654
96-97	0.18896304378504333
98-99	0.19842840572327414
100-101	0.19739582078455803
102-103	0.19670743082541398
104-105	0.2075495726819329
106-107	0.2018703555189944
108-109	0.20858215762064894
110-111	0.19877260070284616
112-113	0.19464226094798182
114-115	0.1955027483969119
116-117	0.19756791827434403
118-119	0.19378177349905173
120-121	0.20100986807006435
122-123	0.1980842107437021
124-125	0.22080107939545596
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	290533.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.49725882800008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.2429253119932	70.15070673267404
2	8.34504599987628	13.268165635674627
3	1.7292357635775684	4.12408509215281
4	0.6365823029378705	2.0242619240790503
5	0.3158952499430884	1.2556403223630739
6	0.17329070572872873	0.8265681651482127
7	0.10860059032395031	0.6043414466499675
8	0.073751304767398	0.4690421251197244
9	0.05721792632421509	0.409380146873781
>10	0.2961173115221513	4.306809529369559
>50	0.014225022003698849	0.7919721293287807
>100	0.006164176201602713	0.7391991173792081
>500	4.741674001232857E-4	0.211468997883598
>1k	4.741674001232857E-4	0.8183586353035497
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2171	0.7472473006508727	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	561	0.19309338353990768	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.4419497957202796E-4
2	0.0	0.0	0.0	0.0	3.4419497957202796E-4
3	0.0	0.0	0.0	0.0	3.4419497957202796E-4
4	0.0	0.0	0.0	0.0	3.4419497957202796E-4
5	0.0	0.0	0.0	0.0	3.4419497957202796E-4
6	0.0	0.0	0.0	0.0	3.4419497957202796E-4
7	0.0	0.0	0.0	0.0	3.4419497957202796E-4
8	0.0	0.0	0.0	0.0	3.4419497957202796E-4
9	0.0	0.0	0.0	0.0	3.4419497957202796E-4
10-11	0.0	0.0	0.0	0.0	3.4419497957202796E-4
12-13	0.0	0.0	0.0	0.0	6.883899591440559E-4
14-15	0.0	0.0	0.0	0.0	6.883899591440559E-4
16-17	0.0	0.0	0.0	3.4419497957202796E-4	6.883899591440559E-4
18-19	0.0	0.0	0.0	5.162924693580419E-4	6.883899591440559E-4
20-21	0.0	0.0	0.0	0.0012046824285020978	6.883899591440559E-4
22-23	0.0	0.0	0.0	0.0030977548161482517	6.883899591440559E-4
24-25	0.0	0.0	0.0	0.006711802101654545	6.883899591440559E-4
26-27	0.0	0.0	0.0	0.012046824285020978	6.883899591440559E-4
28-29	0.0	0.0	0.0	0.025470428488330067	6.883899591440559E-4
30-31	0.0	0.0	0.0	0.05197344191537623	6.883899591440559E-4
32-33	0.0	0.0	0.0	0.09516991185166573	6.883899591440559E-4
34-35	0.0	0.0	0.0	0.14370140397132167	6.883899591440559E-4
36-37	0.0	0.0	0.0	0.20703328021257483	6.883899591440559E-4
38-39	0.0	0.0	0.0	0.3187245510836979	6.883899591440559E-4
40-41	0.0	0.0	0.0	0.43248099183225314	6.883899591440559E-4
42-43	0.0	0.0	0.0	0.5276509036839189	6.883899591440559E-4
44-45	0.0	0.0	0.0	0.6379653946367538	6.883899591440559E-4
46-47	0.0	0.0	0.0	0.7699641693026265	6.883899591440559E-4
48-49	0.0	0.0	0.0	0.8947348493974867	6.883899591440559E-4
50-51	0.0	0.0	0.0	1.0145147022885523	6.883899591440559E-4
52-53	0.0	0.0	0.0	1.1385969924242685	6.883899591440559E-4
54-55	0.0	0.0	0.0	1.2575163578664041	8.604874489300698E-4
56-57	0.0	0.0	0.0	1.3815986480021203	0.0010325849387160838
58-59	0.0	0.0	0.0	1.5351096088912448	0.0010325849387160838
60-61	0.0	0.0	0.0	1.6905136421680154	0.0010325849387160838
62-63	0.0	0.0	0.0	1.8293963164253286	0.0010325849387160838
64-65	0.0	0.0	0.0	1.969139478131572	0.0010325849387160838
66-67	0.0	0.0	0.0	2.11989687918412	0.0010325849387160838
68-69	0.0	0.0	0.0	2.2849383718889076	0.0010325849387160838
70-71	0.0	0.0	0.0	2.4732130257148066	0.0010325849387160838
72-73	0.0	0.0	0.0	2.6408359807663846	0.0010325849387160838
74-75	0.0	0.0	0.0	2.8162033228583327	0.0010325849387160838
76-77	0.0	0.0	0.0	2.9994871494804376	0.0010325849387160838
78-79	0.0	0.0	0.0	3.213920621753811	0.0010325849387160838
80-81	0.0	0.0	0.0	3.4235353643131763	0.0010325849387160838
82-83	0.0	0.0	0.0	3.6644718500135953	0.0010325849387160838
84-85	0.0	0.0	0.0	3.8928452189596365	0.0010325849387160838
86-87	0.0	0.0	0.0	4.119669710497602	0.0010325849387160838
88-89	0.0	0.0	0.0	4.373685605421759	0.0010325849387160838
90-91	0.0	0.0	0.0	4.647148516691736	0.0010325849387160838
92-93	0.0	0.0	0.0	4.922504500349358	0.0010325849387160838
94-95	0.0	0.0	0.0	5.190288194456396	0.0010325849387160838
96-97	0.0	0.0	0.0	5.457555596094076	0.0010325849387160838
98-99	0.0	0.0	0.0	5.748572451322225	0.0010325849387160838
100-101	0.0	0.0	0.0	6.071083147181215	0.0010325849387160838
102-103	0.0	0.0	0.0	6.392561258101489	0.0010325849387160838
104-105	0.0	0.0	0.0	6.704057714614175	0.0010325849387160838
106-107	0.0	0.0	0.0	7.0351732849624655	0.0010325849387160838
108-109	0.0	0.0	0.0	7.397438500962025	0.0010325849387160838
110-111	0.0	0.0	0.0	7.7467964052276335	0.0010325849387160838
112-113	0.0	0.0	0.0	8.135564634654239	0.0010325849387160838
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1295	0.0	41.39865	1
GTATCAA	1920	0.0	36.299286	1
ATCAACG	2355	0.0	29.34645	3
AACGCAG	2395	0.0	29.105082	6
TATCAAC	2390	0.0	28.667408	2
CAACGCA	2390	0.0	28.667408	5
TCAACGC	2390	0.0	28.667408	4
GTATTGC	115	9.963239E-4	25.899158	1
ACGCAGA	2735	0.0	25.051228	7
CGCAGAG	2760	0.0	24.60845	8
CAATACT	125	0.0016181424	23.83134	4
GCAGAGT	2835	0.0	23.74728	9
GTATTTA	135	0.0025338323	22.062246	1
GTACATG	3610	0.0	21.121086	1
CAGAGTA	2830	0.0	21.05242	10-11
GTACAAG	200	4.849959E-5	20.848822	1
TACATGG	3670	0.0	20.77937	2
ATGGGAG	670	0.0	20.45227	5
TACAAGG	205	5.8577083E-5	20.343828	2
ACATGGG	3635	0.0	20.32384	3
>>END_MODULE