##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1142643_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 290533 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.57492264217834 27.0 14.0 33.0 14.0 33.0 2 25.294947561894862 27.0 27.0 33.0 14.0 33.0 3 24.691670137299376 27.0 14.0 33.0 14.0 33.0 4 30.165692021216177 33.0 27.0 33.0 27.0 33.0 5 31.969762471044596 33.0 33.0 33.0 27.0 33.0 6 35.601119322073565 37.0 37.0 37.0 33.0 37.0 7 35.6550615592721 37.0 37.0 37.0 33.0 37.0 8 36.00996788660841 37.0 37.0 37.0 33.0 37.0 9 36.15927966874675 37.0 37.0 37.0 37.0 37.0 10-11 36.123061752021286 37.0 37.0 37.0 37.0 37.0 12-13 36.174808025250144 37.0 37.0 37.0 37.0 37.0 14-15 36.174794257450955 37.0 37.0 37.0 37.0 37.0 16-17 36.17486998034647 37.0 37.0 37.0 37.0 37.0 18-19 36.17110448726994 37.0 37.0 37.0 37.0 37.0 20-21 36.201181965559854 37.0 37.0 37.0 37.0 37.0 22-23 36.180433892191246 37.0 37.0 37.0 37.0 37.0 24-25 36.1555451532184 37.0 37.0 37.0 37.0 37.0 26-27 36.04633897009978 37.0 37.0 37.0 37.0 37.0 28-29 36.00271741936372 37.0 37.0 37.0 37.0 37.0 30-31 36.01176974732647 37.0 37.0 37.0 37.0 37.0 32-33 35.95776727600651 37.0 37.0 37.0 37.0 37.0 34-35 35.84806373114242 37.0 37.0 37.0 33.0 37.0 36-37 35.744600441257965 37.0 37.0 37.0 33.0 37.0 38-39 35.665101726826215 37.0 37.0 37.0 33.0 37.0 40-41 35.57762457276799 37.0 37.0 37.0 33.0 37.0 42-43 35.48435289622866 37.0 37.0 37.0 33.0 37.0 44-45 35.35916746118341 37.0 37.0 37.0 33.0 37.0 46-47 35.16217779047474 37.0 37.0 37.0 33.0 37.0 48-49 34.969950057308466 37.0 37.0 37.0 30.0 37.0 50-51 34.865505811732234 37.0 37.0 37.0 27.0 37.0 52-53 34.79578223472032 37.0 37.0 37.0 27.0 37.0 54-55 34.726084472331884 37.0 37.0 37.0 27.0 37.0 56-57 34.695494143522424 37.0 37.0 37.0 27.0 37.0 58-59 34.65375533932462 37.0 37.0 37.0 27.0 37.0 60-61 34.61416775374914 37.0 37.0 37.0 27.0 37.0 62-63 34.587427245786195 37.0 37.0 37.0 27.0 37.0 64-65 34.575122275266494 37.0 37.0 37.0 27.0 37.0 66-67 34.547992826976625 37.0 37.0 37.0 27.0 37.0 68-69 34.511556346439136 37.0 37.0 37.0 27.0 37.0 70-71 34.46504872079936 37.0 37.0 37.0 27.0 37.0 72-73 34.43185283599453 37.0 37.0 37.0 27.0 37.0 74-75 34.33376759266589 37.0 37.0 37.0 27.0 37.0 76-77 34.27375719797751 37.0 37.0 37.0 27.0 37.0 78-79 34.26642584491263 37.0 37.0 37.0 27.0 37.0 80-81 34.22625656982167 37.0 37.0 37.0 27.0 37.0 82-83 34.182617809336634 37.0 37.0 37.0 27.0 37.0 84-85 34.079244010146866 37.0 37.0 37.0 27.0 37.0 86-87 33.95707888604737 37.0 37.0 37.0 24.5 37.0 88-89 33.897665669648546 37.0 37.0 37.0 22.0 37.0 90-91 33.85224053721952 37.0 37.0 37.0 22.0 37.0 92-93 33.73931016442194 37.0 37.0 37.0 22.0 37.0 94-95 33.75520680955347 37.0 37.0 37.0 22.0 37.0 96-97 33.73370322820472 37.0 37.0 37.0 22.0 37.0 98-99 33.69793792787738 37.0 37.0 37.0 22.0 37.0 100-101 33.591075712569655 37.0 33.0 37.0 22.0 37.0 102-103 33.475224845370406 37.0 33.0 37.0 14.0 37.0 104-105 33.38613513783288 37.0 33.0 37.0 14.0 37.0 106-107 33.28818240957138 37.0 33.0 37.0 14.0 37.0 108-109 33.17858212320115 37.0 33.0 37.0 14.0 37.0 110-111 33.11383904754365 37.0 33.0 37.0 14.0 37.0 112-113 33.033232025277684 37.0 33.0 37.0 14.0 37.0 114-115 32.922390916006094 37.0 33.0 37.0 14.0 37.0 116-117 32.82729156412525 37.0 33.0 37.0 14.0 37.0 118-119 32.69476445016573 37.0 33.0 37.0 14.0 37.0 120-121 32.61284432405269 37.0 33.0 37.0 14.0 37.0 122-123 32.487803450898866 37.0 33.0 37.0 14.0 37.0 124-125 31.233083677241485 37.0 27.5 37.0 14.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 3.0 15 66.0 16 35.0 17 31.0 18 53.0 19 221.0 20 902.0 21 2255.0 22 3654.0 23 4835.0 24 3853.0 25 2863.0 26 2507.0 27 2903.0 28 3517.0 29 4363.0 30 5840.0 31 7835.0 32 11007.0 33 16552.0 34 27689.0 35 61843.0 36 127706.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.609608027465285 10.74506806059166 12.183414083187847 20.46190982875521 2 19.102477171267978 19.489008133327367 36.30121191052307 25.10730278488158 3 22.471801826298563 25.266665060423428 28.789844871322707 23.471688241955302 4 15.106769650159018 18.0145388459791 34.46986906777911 32.40882243608277 5 15.472203386330078 35.8545509618926 31.35780201775431 17.315443634023016 6 28.982449377174895 33.695543676922604 18.854786580433757 18.467220365468744 7 25.745096082028546 33.54971724382428 21.95069062722651 18.75449604692066 8 26.86958108029036 30.66811687484726 20.99623794887328 21.466064095989097 9 28.278371131678675 15.599949059143023 20.437265302048306 35.68441450712999 10-11 26.074834872458553 25.188704897550362 25.454767616759543 23.281692613231545 12-13 27.448172840950942 24.516836297425765 26.2748121555899 21.760178706033393 14-15 25.808427958269803 23.97042676735517 25.87709485669442 24.344050417680606 16-17 23.24761731025391 26.605583530958615 26.648435805915334 23.498363352872133 18-19 23.062268313754373 24.323570816396074 30.302237611562198 22.311923258287354 20-21 25.25053135191416 22.620016693485237 30.260642096839423 21.86880985776118 22-23 25.180616315530425 22.64062946377864 29.98488984039679 22.193864380294148 24-25 23.706429218023427 23.483218773770968 30.12979592679661 22.680556081408998 26-27 23.131795699627926 23.717615554859517 30.308433121194494 22.842155624318064 28-29 23.017867161389585 23.65273480121019 30.679647406662923 22.6497506307373 30-31 23.56548137388868 23.31852147604575 30.766556639004865 22.349440511060706 32-33 23.19447910302634 23.354357946185022 30.232590157727625 23.218572793061018 34-35 23.31184979305241 23.568963885279615 30.53531016323475 22.58387615843322 36-37 23.25581395348837 23.802918464390018 30.135149786854694 22.806117795266918 38-39 23.284818633499754 23.510440384056064 30.09718428874077 23.107556693703415 40-41 23.029515532226142 23.750623870579123 30.01428448498408 23.20557611221065 42-43 23.255773930403056 23.67380304959901 29.79055519223488 23.279867827763052 44-45 23.184841627012467 23.68977653102493 29.607703096899655 23.517678745062945 46-47 23.24786942574312 23.678630925336968 29.41913455316454 23.654365095755374 48-49 23.2621135329094 24.175264385223684 29.124280416132446 23.43834166573447 50-51 23.358275996186322 24.427861895206394 28.62170562380177 23.592156484805514 52-53 23.367569260634767 24.183655557200044 28.490739434074612 23.958035748090577 54-55 23.07181308771495 24.133486156430273 28.692536450373794 24.10216430548098 56-57 23.25195976353764 24.120709387073735 28.094275167149974 24.533055682238647 58-59 23.209227242531778 24.171086734289972 28.538090599626205 24.08159542355205 60-61 23.480373934712873 23.952094777855795 28.92073850729007 23.646792780141258 62-63 22.918222714803484 23.83309297050593 29.06898699975562 24.179697314934963 64-65 22.828215727645397 23.80332010477295 29.17362227354552 24.194841894036134 66-67 23.227866073502966 23.8121380568439 28.9900441430821 23.969951726571036 68-69 23.113553365710608 23.67820522969852 29.101685522814964 24.10655588177591 70-71 22.919545729120593 23.762311487738852 29.367727767901243 23.95041501523931 72-73 23.00620412518393 24.122430364933358 29.177286534208736 23.69407897567398 74-75 22.938014258425785 23.8865664546333 29.41545277133295 23.759966515607964 76-77 22.74608025191121 23.538298290159545 29.61386476309253 24.101756694836716 78-79 22.86812766968069 23.993825099559082 29.356247698180226 23.78179953258 80-81 22.728571281059295 23.950635556029777 29.55447401844197 23.76631914446896 82-83 22.762724932193358 23.837133258986963 29.71531535252571 23.68482645629397 84-85 22.72027152751456 24.100995830605424 29.17576927052824 24.00296337135178 86-87 22.17239119939306 24.4108214359611 29.245982481550453 24.170804883095386 88-89 22.414578273349527 23.833386233164052 29.29368376021197 24.45835173327445 90-91 22.845612336820132 23.637763512128576 29.141138637651554 24.375485513399738 92-93 22.845777043913504 23.953180531760506 28.900214758715308 24.300827665610683 94-95 22.40065122474654 24.130423932853635 28.913070789713263 24.55585405268656 96-97 22.38678566634427 24.133919382651882 28.60931460453718 24.869980346466665 98-99 22.157896004928872 23.986431833905268 28.523437957133957 25.3322342040319 100-101 22.4934861100116 24.433024819899977 28.15033059927788 24.923158470810545 102-103 22.691054028285944 24.235284804135848 28.31606048194181 24.7576006856364 104-105 22.82283005846403 24.145972487311568 28.172366493930724 24.85883096029368 106-107 22.84827195701449 24.15921298790258 28.12645753339311 24.866057521689818 108-109 22.665702827834288 24.242439889158536 27.85253265864615 25.239324624361025 110-111 22.341701675791125 24.704365799886066 27.732561841804078 25.221370682518728 112-113 22.249453134815393 24.8346496534656 27.72774288817102 25.18815432354799 114-115 22.381933365344402 24.844032086794744 27.625380991964533 25.148653555896317 116-117 22.163383597619532 24.727653847610064 27.662452543790504 25.446510010979896 118-119 22.072075172959764 24.576635803531477 27.86235500636767 25.488934017141084 120-121 22.22019529623141 24.559172279913124 28.09319423266892 25.127438191186542 122-123 22.259261426412834 24.708208706067815 27.79925172011441 25.233278147404942 124-125 22.000464664022097 24.766076084431173 27.658695670880196 25.574763580666538 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 292.0 1 236.0 2 361.5 3 491.0 4 352.5 5 316.0 6 426.0 7 799.0 8 1390.0 9 1680.5 10 1458.5 11 1029.5 12 795.0 13 742.0 14 724.0 15 712.0 16 671.0 17 631.5 18 621.5 19 618.0 20 612.5 21 625.5 22 618.0 23 625.0 24 684.0 25 735.0 26 795.0 27 885.0 28 1041.5 29 1289.0 30 1579.5 31 2006.0 32 2466.0 33 2847.0 34 3267.0 35 3722.0 36 4322.5 37 5037.5 38 5491.5 39 5640.0 40 5724.5 41 5852.0 42 6160.0 43 6625.0 44 7282.5 45 7994.5 46 8891.5 47 9913.0 48 10878.0 49 11819.5 50 12639.5 51 12670.5 52 12243.5 53 12218.5 54 12130.0 55 11744.0 56 11234.5 57 10520.0 58 9516.0 59 8344.0 60 7313.5 61 6138.5 62 4821.5 63 3737.0 64 2873.5 65 2353.0 66 1878.5 67 1468.0 68 1199.5 69 970.5 70 808.0 71 692.5 72 611.0 73 587.0 74 479.0 75 403.5 76 343.5 77 250.5 78 196.5 79 134.0 80 99.0 81 89.5 82 77.5 83 54.0 84 27.5 85 14.0 86 14.0 87 6.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04474534734436363 2 0.0 3 0.0 4 3.4419497957202796E-4 5 0.004130339754864336 6 6.883899591440559E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.7209748978601398E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 1.7209748978601398E-4 34-35 1.7209748978601398E-4 36-37 0.0012046824285020978 38-39 8.604874489300699E-4 40-41 0.0027535598365762237 42-43 0.001032584938716084 44-45 1.7209748978601398E-4 46-47 3.4419497957202796E-4 48-49 1.7209748978601398E-4 50-51 0.0 52-53 0.0 54-55 3.4419497957202796E-4 56-57 1.7209748978601398E-4 58-59 6.883899591440559E-4 60-61 3.4419497957202796E-4 62-63 0.0 64-65 0.0 66-67 1.7209748978601398E-4 68-69 0.0 70-71 5.16292469358042E-4 72-73 1.7209748978601398E-4 74-75 0.08553245242364894 76-77 0.0936210344435916 78-79 6.883899591440559E-4 80-81 0.0 82-83 3.4419497957202796E-4 84-85 0.11117497840176503 86-87 0.19033982370333147 88-89 0.23302000117026295 90-91 0.08518825744407693 92-93 0.1514457910116923 94-95 0.0012046824285020978 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0043024372446503496 106-107 0.005679217162938462 108-109 0.009637459428016783 110-111 0.004646632224222377 112-113 0.004646632224222377 114-115 0.0029256573263622375 116-117 6.883899591440559E-4 118-119 0.001032584938716084 120-121 0.0 122-123 0.0 124-125 1.7209748978601398E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 290533.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.34537781651092 #Duplication Level Percentage of deduplicated Percentage of total 1 87.80142274157356 67.03232792032627 2 8.513954241742692 13.00002106596663 3 1.8667005055363541 4.275418660963346 4 0.6516141335827222 1.9899090887580537 5 0.324371198808922 1.2382120862930863 6 0.19463743012392512 0.8915800884027483 7 0.13116936207817614 0.7009922154066355 8 0.08340190345446102 0.5093879863877595 9 0.06606755666842218 0.4539557317737993 >10 0.3304056925969012 4.480858551350344 >50 0.0222365434181104 1.1540379139037924 >100 0.011601674826840137 1.5673805629515134 >500 9.668062355700115E-4 0.4871538363776778 >1k 0.0014502093533550172 2.2187642911383327 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2519 0.8670271535419385 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2434 0.8377705802783161 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1059 0.3645024833667776 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 769 0.2646859392908895 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 551 0.18965143374418741 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 366 0.12597536252336225 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 313 0.10773302860604475 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 3.4419497957202796E-4 0.0 0.0 0.0 0.0 9 3.4419497957202796E-4 0.0 0.0 0.0 0.0 10-11 3.4419497957202796E-4 0.0 0.0 0.0 0.0 12-13 3.4419497957202796E-4 0.0 0.0 0.0 0.0 14-15 3.4419497957202796E-4 0.0 0.0 0.0 3.4419497957202796E-4 16-17 6.883899591440559E-4 0.0 0.0 3.4419497957202796E-4 3.4419497957202796E-4 18-19 6.883899591440559E-4 0.0 0.0 5.162924693580419E-4 3.4419497957202796E-4 20-21 6.883899591440559E-4 0.0 0.0 0.0012046824285020978 3.4419497957202796E-4 22-23 6.883899591440559E-4 0.0 0.0 0.0027535598365762237 3.4419497957202796E-4 24-25 6.883899591440559E-4 0.0 0.0 0.006023412142510489 3.4419497957202796E-4 26-27 6.883899591440559E-4 0.0 0.0 0.01118633683609091 3.4419497957202796E-4 28-29 6.883899591440559E-4 0.0 0.0 0.024093648570041957 3.4419497957202796E-4 30-31 6.883899591440559E-4 0.0 0.0 0.05076875948687412 3.4419497957202796E-4 32-33 6.883899591440559E-4 0.0 0.0 0.09448152189252168 3.4419497957202796E-4 34-35 6.883899591440559E-4 0.0 0.0 0.1431851115019636 3.4419497957202796E-4 36-37 6.883899591440559E-4 0.0 0.0 0.20720537770236083 3.4419497957202796E-4 38-39 6.883899591440559E-4 0.0 0.0 0.32027342849177204 3.4419497957202796E-4 40-41 6.883899591440559E-4 0.0 0.0 0.4350624541790433 3.4419497957202796E-4 42-43 6.883899591440559E-4 0.0 0.0 0.5312649509694252 3.4419497957202796E-4 44-45 6.883899591440559E-4 0.0 0.0 0.6427841243507622 3.4419497957202796E-4 46-47 6.883899591440559E-4 0.0 0.0 0.7763317764247091 6.883899591440559E-4 48-49 6.883899591440559E-4 0.0 0.0 0.9026513339276433 0.0010325849387160838 50-51 6.883899591440559E-4 0.0 0.0 1.023463771757425 0.0013767799182881119 52-53 6.883899591440559E-4 0.0 0.0 1.1489228418114292 0.0013767799182881119 54-55 6.883899591440559E-4 0.0 0.0 1.2699073771309972 0.0013767799182881119 56-57 6.883899591440559E-4 0.0 0.0 1.3946780572258572 0.0013767799182881119 58-59 6.883899591440559E-4 0.0 0.0 1.54870531058434 0.0013767799182881119 60-61 8.604874489300698E-4 0.0 0.0 1.7054861237793986 0.0013767799182881119 62-63 0.0010325849387160838 0.0 0.0 1.844540895526498 0.0017209748978601399 64-65 0.0010325849387160838 0.0 0.0 1.9841119597429553 0.0017209748978601399 66-67 0.0010325849387160838 0.0 0.0 2.1355577507546473 0.0020651698774321677 68-69 0.0010325849387160838 0.0 0.0 2.3038690957653696 0.0020651698774321677 70-71 0.0010325849387160838 0.0 0.0 2.4919716521014825 0.0020651698774321677 72-73 0.0010325849387160838 0.0 0.0 2.660627192091776 0.0020651698774321677 74-75 0.0010325849387160838 0.0 0.0 2.838920191510087 0.0020651698774321677 76-77 0.0010325849387160838 0.0 0.0 3.02358079805048 0.0020651698774321677 78-79 0.0010325849387160838 0.0 0.0 3.240251537691071 0.0020651698774321677 80-81 0.0010325849387160838 0.0 0.0 3.448661597821934 0.0020651698774321677 82-83 0.0010325849387160838 0.0 0.0 3.690286473481498 0.0020651698774321677 84-85 0.0010325849387160838 0.0 0.0 3.9193482323866826 0.0020651698774321677 86-87 0.0010325849387160838 0.0 0.0 4.147205308863365 0.0020651698774321677 88-89 0.0010325849387160838 0.0 0.0 4.401909593746666 0.0020651698774321677 90-91 0.0010325849387160838 0.0 0.0 4.67502831003707 0.0020651698774321677 92-93 0.0010325849387160838 0.0 0.0 4.949523806245763 0.0024093648570041957 94-95 0.0010325849387160838 0.0 0.0 5.221093645128092 0.0024093648570041957 96-97 0.0010325849387160838 0.0 0.0 5.492147191541065 0.0024093648570041957 98-99 0.0010325849387160838 0.0 0.0 5.785573411626218 0.0024093648570041957 100-101 0.0010325849387160838 0.0 0.0 6.109116692423925 0.0024093648570041957 102-103 0.0010325849387160838 0.0 0.0 6.43610192301735 0.0024093648570041957 104-105 0.0010325849387160838 0.0 0.0 6.752245011754258 0.0024093648570041957 106-107 0.0010325849387160838 0.0 0.0 7.084565264531052 0.0024093648570041957 108-109 0.0010325849387160838 0.0 0.0 7.451477112754834 0.0024093648570041957 110-111 0.0010325849387160838 0.0 0.0 7.80909569653017 0.0024093648570041957 112-113 0.0010325849387160838 0.0 0.0 8.199584900854637 0.0024093648570041957 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCAACG 145 0.0 73.88624 1 GCGCAGA 205 0.0 69.68133 1 GCGTACA 35 9.12613E-5 68.02225 1 CGTACAT 40 1.7744445E-4 59.46825 2 AACGCGT 30 0.0042423285 59.468246 6 GGTATCA 1735 0.0 55.91742 1 ACGCGTG 35 0.0078068823 50.972782 7 GTATCAA 2810 0.0 45.116177 1 ATCAACG 3375 0.0 37.35487 3 TATCAAC 3470 0.0 36.332184 2 CAACGCA 3570 0.0 36.14736 5 TCAACGC 3605 0.0 35.63146 4 AACGCAG 3670 0.0 35.162426 6 CGTACTT 85 1.712711E-4 34.98132 2 ACGCAGA 4050 0.0 31.863234 7 GAATAGT 75 0.003899464 31.743715 1 CGCAGAG 4245 0.0 30.39955 8 GCGTACT 100 4.422075E-4 29.759733 1 GCAGAGT 4490 0.0 29.00567 9 TGGTATC 920 0.0 28.441334 2 >>END_MODULE