FastQCFastQC Report
Sun 1 Jan 2017
ERR1142642_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142642_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435563
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8920.20479241808877247No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8720.20020065983566096No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAT250.00204526271.494146
TCTAACG700.002770698634.04092
GTACTAG1152.7396125E-531.0772341
GTATCAA25650.029.2598551
GGTATCA19750.025.635441
AACGCAG28400.025.1739966
ATCAACG29400.024.9228043
CAACGCA29600.024.5559845
TCAACGC30550.024.1796264
TATCAAC30950.023.8671262
GGGCTAT2052.2319946E-623.2501286
ATGGGTA3705.456968E-1222.54325
ACGCAGA32100.022.272327
CTAGGAC1350.00253497722.0635473
CGCAGAG32450.022.0320958
ATAGGAC3255.966285E-1021.995663
ATTTAGG2204.1284165E-621.6598911
TAGGACC3401.0477379E-921.0252634
CATGGGT9650.020.988954
CTATTGA2306.055212E-620.7229429
Produced by FastQC (version 0.11.5)