FastQCFastQC Report
Sun 1 Jan 2017
ERR1142642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435563
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21510.49384360012214074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21490.4933844242968296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8290.19032837959147125No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.14441079706035637No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5030.11548272006575398No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG1650.061.2983551
GCGCAGA3050.060.4708021
TCTAACG653.5253186E-545.749982
GGTATCA21200.038.16691
GTATCAA30000.035.498961
CTAACGC700.002792847833.98573
ATCAACG35250.029.8640293
CAACGCA36850.028.890155
TCAACGC37450.028.5861034
AACGCAG38000.028.4853826
TATCAAC37250.028.260592
GTGTTAT1556.8226163E-626.868921
AGTACTA900.00954213626.4333215
ACGCAGA41150.026.1603167
CGCAGAG43950.024.358358
GCAGAGT46700.022.9239739
GAGTACT19250.022.55414612-13
GCGGTAT1852.6871116E-522.5117971
TAACGCC2153.4267432E-622.1302244
GTCTAAC1400.00315394521.248381
Produced by FastQC (version 0.11.5)