##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142642_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	435563
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.465491788788302	27.0	14.0	33.0	14.0	33.0
2	25.545718988986668	27.0	27.0	33.0	14.0	33.0
3	24.923875535800793	27.0	14.0	33.0	14.0	33.0
4	30.27234636550855	33.0	27.0	33.0	27.0	33.0
5	31.972692813668747	33.0	33.0	33.0	33.0	33.0
6	35.616657062238986	37.0	37.0	37.0	33.0	37.0
7	35.65634592469976	37.0	37.0	37.0	33.0	37.0
8	35.990889951625824	37.0	37.0	37.0	33.0	37.0
9	36.152159389112484	37.0	37.0	37.0	37.0	37.0
10-11	36.125844022563896	37.0	37.0	37.0	37.0	37.0
12-13	36.17438235111798	37.0	37.0	37.0	37.0	37.0
14-15	36.173819860731975	37.0	37.0	37.0	37.0	37.0
16-17	36.16530903680983	37.0	37.0	37.0	37.0	37.0
18-19	36.16790338022284	37.0	37.0	37.0	37.0	37.0
20-21	36.17948609041631	37.0	37.0	37.0	37.0	37.0
22-23	36.17257319836625	37.0	37.0	37.0	37.0	37.0
24-25	36.15196423938673	37.0	37.0	37.0	37.0	37.0
26-27	36.042020327713786	37.0	37.0	37.0	37.0	37.0
28-29	36.00792422680531	37.0	37.0	37.0	37.0	37.0
30-31	36.02594458206964	37.0	37.0	37.0	37.0	37.0
32-33	35.979591930444045	37.0	37.0	37.0	37.0	37.0
34-35	35.89364110358318	37.0	37.0	37.0	33.0	37.0
36-37	35.81256672398712	37.0	37.0	37.0	33.0	37.0
38-39	35.75331697136809	37.0	37.0	37.0	33.0	37.0
40-41	35.67389562474315	37.0	37.0	37.0	33.0	37.0
42-43	35.58530453688674	37.0	37.0	37.0	33.0	37.0
44-45	35.50647437913689	37.0	37.0	37.0	33.0	37.0
46-47	35.37244784336594	37.0	37.0	37.0	33.0	37.0
48-49	35.240815909523995	37.0	37.0	37.0	33.0	37.0
50-51	35.14359920378912	37.0	37.0	37.0	33.0	37.0
52-53	35.08632620309806	37.0	37.0	37.0	33.0	37.0
54-55	35.01463852531092	37.0	37.0	37.0	33.0	37.0
56-57	34.98098782495299	37.0	37.0	37.0	30.0	37.0
58-59	34.92847188581216	37.0	37.0	37.0	27.0	37.0
60-61	34.9134063269837	37.0	37.0	37.0	27.0	37.0
62-63	34.89488661800934	37.0	37.0	37.0	27.0	37.0
64-65	34.87822771906705	37.0	37.0	37.0	27.0	37.0
66-67	34.844919104698974	37.0	37.0	37.0	27.0	37.0
68-69	34.79217472558504	37.0	37.0	37.0	27.0	37.0
70-71	34.751236904879434	37.0	37.0	37.0	27.0	37.0
72-73	34.73156810840223	37.0	37.0	37.0	27.0	37.0
74-75	34.639447106388744	37.0	37.0	37.0	27.0	37.0
76-77	34.58637097274103	37.0	37.0	37.0	27.0	37.0
78-79	34.5562777370897	37.0	37.0	37.0	27.0	37.0
80-81	34.52401259978465	37.0	37.0	37.0	27.0	37.0
82-83	34.499081648349375	37.0	37.0	37.0	27.0	37.0
84-85	34.38770969985972	37.0	37.0	37.0	27.0	37.0
86-87	34.278044737500664	37.0	37.0	37.0	27.0	37.0
88-89	34.22290460851817	37.0	37.0	37.0	27.0	37.0
90-91	34.17513654741106	37.0	37.0	37.0	27.0	37.0
92-93	34.074866322437856	37.0	37.0	37.0	27.0	37.0
94-95	34.10182109132319	37.0	37.0	37.0	27.0	37.0
96-97	34.100538842831	37.0	37.0	37.0	27.0	37.0
98-99	34.031366300627	37.0	37.0	37.0	24.5	37.0
100-101	33.94779515248081	37.0	37.0	37.0	22.0	37.0
102-103	33.87033793044864	37.0	37.0	37.0	22.0	37.0
104-105	33.76484458046252	37.0	35.0	37.0	22.0	37.0
106-107	33.66886764945599	37.0	33.0	37.0	22.0	37.0
108-109	33.56661952461527	37.0	33.0	37.0	22.0	37.0
110-111	33.50641009452134	37.0	33.0	37.0	22.0	37.0
112-113	33.43914772375064	37.0	33.0	37.0	22.0	37.0
114-115	33.35134297449508	37.0	33.0	37.0	18.0	37.0
116-117	33.260406646110894	37.0	33.0	37.0	14.0	37.0
118-119	33.15855341247994	37.0	33.0	37.0	14.0	37.0
120-121	33.079782947587375	37.0	33.0	37.0	14.0	37.0
122-123	32.96217883520869	37.0	33.0	37.0	14.0	37.0
124-125	31.707654231420022	37.0	30.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	174.0
16	91.0
17	16.0
18	80.0
19	295.0
20	1061.0
21	2702.0
22	4207.0
23	5450.0
24	4576.0
25	3469.0
26	3240.0
27	3923.0
28	5080.0
29	6328.0
30	8589.0
31	11585.0
32	16112.0
33	24344.0
34	40917.0
35	90545.0
36	202777.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.30750892278859	11.352267559634544	11.716528633308988	21.62369488426787
2	18.741490897987205	19.511528757034	35.516331736166755	26.23064860881204
3	22.531757748018084	24.040150334165205	28.548108999157414	24.879982918659298
4	15.21830466915082	17.425802585630944	34.87134982241293	32.4845429228053
5	16.055369200709908	35.093181249411664	31.682543181134616	17.168906368743816
6	30.115483515474335	34.00289282762421	18.5708053999449	17.310818256956562
7	26.44393577966907	31.996519447244143	22.562292940401274	18.997251832685514
8	26.30664220790104	31.014342356903597	21.363614448426517	21.315400986768847
9	27.065889435053027	15.783709819245436	21.16157708528961	35.98882366041193
10-11	25.710172810821856	24.71135059681378	26.47217509292571	23.106301499438658
12-13	26.78854723656509	23.79345812201679	26.640463032902247	22.777531608515876
14-15	25.083742191141123	24.054269990793525	25.33031960933321	25.531668208732146
16-17	23.77026974283858	26.168774666351364	26.323172537612237	23.737783053197813
18-19	23.58855090997169	24.743378110629234	28.73545273588436	22.932618243514717
20-21	24.945587289925612	23.66665901368353	28.591927633391496	22.795826062999357
22-23	24.719845349582034	23.529776404331866	28.575429960763426	23.174948285322674
24-25	23.731125003730806	24.39176422239722	28.571412172291954	23.305698601580023
26-27	23.496715744909462	24.458803892892647	28.60470241962701	23.43977794257088
28-29	23.250138326717376	24.29476332930024	29.13596884951201	23.319129494470374
30-31	23.69450573166224	24.11166696895742	28.995346253010474	23.19848104636987
32-33	23.3027139587155	24.213604002176496	28.644650716429084	23.839031322678924
34-35	23.55342395933539	24.229789950018713	28.7905538349217	23.4262322557242
36-37	23.55567290197046	24.407569609064243	28.42965624515707	23.607101243808224
38-39	23.67862484918823	24.124947337849363	28.484397654051858	23.71203015891055
40-41	23.34183980962024	24.458472668496533	28.496465966633032	23.703221555250195
42-43	23.730025714023327	24.32317017173294	28.36658554504546	23.580218569198273
44-45	23.543031761264757	24.510287857985773	27.908655943355942	24.038024437393528
46-47	23.60533792509686	24.446089826373942	27.901621466494475	24.046950782034727
48-49	23.821467847360772	24.855187424092495	27.627117087539578	23.696227641007155
50-51	23.592109522617854	24.99822069367692	27.403383666656715	24.006286117048507
52-53	23.591420758879885	24.78699981403379	27.33393332307841	24.287646104007916
54-55	23.691231099131695	24.816558836629458	27.342261262460916	24.14994880177793
56-57	23.792769358278825	24.733391036428714	26.952013830375858	24.521825774916604
58-59	23.55226937214305	24.71800735143872	27.382961284412517	24.34676199200571
60-61	23.634468492502805	24.729602835869898	27.643532623294448	23.992396048332846
62-63	23.257370345966024	24.94174206716365	27.507386991089692	24.293500595780635
64-65	23.43885959092026	24.526187945257057	27.62252532928646	24.412427134536223
66-67	23.64987803128139	24.68291003013345	27.53141053235758	24.135801406227582
68-69	23.677286638213072	24.50070368695229	27.418880850760967	24.40312882407367
70-71	23.256038203691798	24.553678023693635	27.90625860960602	24.28402516300854
72-73	23.656306500215237	24.67108623905869	27.795322140909747	23.87728511981633
74-75	23.619025735294116	24.517348345588236	27.919462316176467	23.944163602941178
76-77	23.42715070101645	24.65018112799137	27.894400025735916	24.028268145256263
78-79	23.54144496905704	24.786165839569566	27.723192812922232	23.949196378451166
80-81	23.215814933775366	24.85656495156843	27.99468733570115	23.932932778955056
82-83	23.390152710926014	24.921279773350033	27.82959467262373	23.85897284310023
84-85	23.38598573593673	24.992560028220186	27.472857029595577	24.14859720624751
86-87	23.11748884286639	25.0016386411123	27.711088598162654	24.16978391785866
88-89	23.372261027212573	24.59266263585581	27.491188352981233	24.54388798395038
90-91	23.656306793769833	24.72549438581738	27.459866693318812	24.158332127093974
92-93	23.498906728162726	24.956257630485617	27.268452842161896	24.27638279918976
94-95	22.909896144834736	25.060812726648656	27.48172748321982	24.547563645296787
96-97	23.198136664500886	24.908337025872264	27.256103020688165	24.637423288938685
98-99	23.0184841228479	25.00453436127495	27.160135273198136	24.816846242679016
100-101	23.16622394464176	25.3104602548885	26.895420409906258	24.627895390563477
102-103	23.523260152102164	25.092954512842592	26.905093987659633	24.47869134739561
104-105	23.258040519442353	25.262889627684	26.86169480006612	24.617375052807525
106-107	23.29391127856492	25.19843046495983	26.83741903784471	24.670239218630538
108-109	23.417599448940933	25.429653866023767	26.488835313701852	24.663911371333448
110-111	23.148045042262996	25.64226781755794	26.413023638119004	24.796663502060056
112-113	23.07618130325924	25.64467858196541	26.389834380485205	24.88930573429014
114-115	23.169291135200787	25.610236288266403	26.53412880540284	24.686343771129966
116-117	23.317285064548937	25.70753579865966	26.25969726398828	24.71548187280312
118-119	22.93007376727378	25.542349027341878	26.540491644071185	24.98708556131316
120-121	23.042246471807754	25.448901766219812	26.78659573930752	24.72225602266492
122-123	23.016188243721345	25.652087068919993	26.62083326637019	24.710891420988467
124-125	22.98708335437894	25.58992749597072	26.57118848751728	24.851800662133062
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	96.0
1	98.5
2	201.5
3	313.0
4	272.0
5	247.5
6	321.0
7	572.0
8	943.5
9	1195.5
10	1104.5
11	884.5
12	845.5
13	822.5
14	800.0
15	789.0
16	785.5
17	794.0
18	790.0
19	790.0
20	795.5
21	788.5
22	790.5
23	824.0
24	859.5
25	951.5
26	1134.5
27	1338.5
28	1596.0
29	2024.5
30	2628.5
31	3278.5
32	4064.5
33	4703.0
34	5372.0
35	6124.0
36	6851.0
37	7797.5
38	8634.0
39	9015.0
40	9080.5
41	9319.5
42	9867.5
43	10944.0
44	12082.0
45	13055.0
46	14418.0
47	15739.5
48	16938.0
49	18219.0
50	18929.5
51	18296.5
52	17510.0
53	17559.5
54	17421.0
55	17004.0
56	16404.5
57	15115.5
58	13996.0
59	12732.0
60	11255.0
61	9539.5
62	7594.5
63	5990.0
64	4581.5
65	3698.0
66	2890.0
67	2204.5
68	1850.0
69	1541.5
70	1266.0
71	1147.5
72	1027.5
73	973.0
74	814.0
75	657.0
76	530.5
77	366.5
78	267.0
79	168.0
80	101.5
81	77.5
82	68.0
83	52.5
84	31.0
85	18.5
86	12.0
87	4.0
88	3.0
89	4.5
90	3.0
91	1.0
92	1.5
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0369636539375475
2	0.0
3	0.0
4	4.5917582531114905E-4
5	0.003214230777178043
6	6.887637379667235E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.887637379667235E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0012627335196056598
38-39	8.035576942945108E-4
40-41	0.0028698489081946813
42-43	6.887637379667235E-4
44-45	2.2958791265557452E-4
46-47	1.1479395632778726E-4
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.2958791265557452E-4
56-57	0.0
58-59	4.5917582531114905E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.1479395632778726E-4
68-69	0.0
70-71	6.887637379667235E-4
72-73	1.1479395632778726E-4
74-75	0.08334041229397354
76-77	0.08575108537685708
78-79	8.035576942945108E-4
80-81	0.0
82-83	3.4438186898336177E-4
84-85	0.09493460188308006
86-87	0.17253531636066424
88-89	0.20915458842922838
90-91	0.07484565952571728
92-93	0.14464038497301193
94-95	0.0010331456069500853
96-97	0.0
98-99	0.0
100-101	0.0
102-103	1.1479395632778726E-4
104-105	0.0043621703404559155
106-107	0.00459175825311149
108-109	0.008724340680911831
110-111	0.0037882005588169795
112-113	0.0037882005588169795
114-115	0.0033290247335058306
116-117	4.5917582531114905E-4
118-119	9.183516506222981E-4
120-121	0.0
122-123	0.0
124-125	2.2958791265557452E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	435563.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.98729940956832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.48290768741602	68.12060106760292
2	8.10776281496246	12.483895267545588
3	1.661707007483103	3.8379100474823833
4	0.5953761611993554	1.8334561113429675
5	0.3084375557150396	1.1872887225494582
6	0.17679223764145488	0.8166454159554144
7	0.12243106093126387	0.6597945721462422
8	0.07854881202341354	0.48378087276099513
9	0.05490299607012946	0.3804150057240069
>10	0.3627621052865005	5.278226926160136
>50	0.03369329442292407	1.7379503489884085
>100	0.013010281979247135	1.5720399097964373
>500	0.0010007909214805489	0.5036383373812797
>1k	6.671939476536991E-4	1.1043573945637446
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2151	0.49384360012214074	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2149	0.4933844242968296	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	829	0.19032837959147125	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	629	0.14441079706035637	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	503	0.11548272006575398	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.295879126555745E-4	0.0
2	0.0	0.0	0.0	2.295879126555745E-4	0.0
3	0.0	0.0	0.0	2.295879126555745E-4	0.0
4	0.0	0.0	0.0	2.295879126555745E-4	0.0
5	0.0	0.0	0.0	2.295879126555745E-4	0.0
6	0.0	0.0	0.0	2.295879126555745E-4	0.0
7	0.0	0.0	0.0	2.295879126555745E-4	0.0
8	0.0	0.0	0.0	2.295879126555745E-4	0.0
9	0.0	0.0	0.0	2.295879126555745E-4	0.0
10-11	0.0	0.0	0.0	4.59175825311149E-4	0.0
12-13	0.0	0.0	0.0	6.887637379667235E-4	1.1479395632778725E-4
14-15	0.0	0.0	0.0	6.887637379667235E-4	2.295879126555745E-4
16-17	0.0	0.0	0.0	9.183516506222981E-4	2.295879126555745E-4
18-19	0.0	0.0	0.0	0.0011479395632778726	2.295879126555745E-4
20-21	0.0	0.0	0.0	0.0016071153885890215	2.295879126555745E-4
22-23	0.0	0.0	0.0	0.003099436820850256	2.295879126555745E-4
24-25	0.0	0.0	0.0	0.0057396978163893626	2.295879126555745E-4
26-27	0.0	0.0	0.0	0.009987074200517492	2.295879126555745E-4
28-29	0.0	0.0	0.0	0.02135167587696843	2.295879126555745E-4
30-31	0.0	0.0	0.0	0.03971870888941439	2.295879126555745E-4
32-33	0.0	0.0	0.0	0.07346813204978385	2.295879126555745E-4
34-35	0.0	0.0	0.0	0.10756193707913667	2.295879126555745E-4
36-37	0.0	0.0	0.0	0.15474225312985723	2.295879126555745E-4
38-39	0.0	0.0	0.0	0.22729203352901878	2.295879126555745E-4
40-41	0.0	0.0	0.0	0.3058110996572252	2.295879126555745E-4
42-43	0.0	0.0	0.0	0.38042717127028697	2.295879126555745E-4
44-45	0.0	0.0	0.0	0.4564207703592821	2.295879126555745E-4
46-47	0.0	0.0	0.0	0.554684396975868	2.295879126555745E-4
48-49	0.0	0.0	0.0	0.6450272406058366	2.295879126555745E-4
50-51	0.0	0.0	0.0	0.7336481748908883	4.59175825311149E-4
52-53	0.0	0.0	0.0	0.8224986970885957	6.887637379667235E-4
54-55	0.0	0.0	0.0	0.9056095214699136	6.887637379667235E-4
56-57	0.0	0.0	0.0	1.0043323239118107	6.887637379667235E-4
58-59	0.0	0.0	0.0	1.108335648344786	6.887637379667235E-4
60-61	0.0	0.0	0.0	1.2186526403757894	6.887637379667235E-4
62-63	0.0	0.0	0.0	1.3185233823809643	6.887637379667235E-4
64-65	0.0	0.0	0.0	1.4121952507444386	6.887637379667235E-4
66-67	0.0	0.0	0.0	1.519986775736231	6.887637379667235E-4
68-69	0.0	0.0	0.0	1.6452269820898469	6.887637379667235E-4
70-71	0.0	0.0	0.0	1.7857347846350584	6.887637379667235E-4
72-73	0.0	0.0	0.0	1.9156815431981138	6.887637379667235E-4
74-75	0.0	0.0	0.0	2.041266131420713	6.887637379667235E-4
76-77	0.0	0.0	0.0	2.1709833020711127	6.887637379667235E-4
78-79	0.0	0.0	0.0	2.315279305175141	6.887637379667235E-4
80-81	0.0	0.0	0.0	2.4808121901998104	8.035576942945108E-4
82-83	0.0	0.0	0.0	2.658054058769914	9.18351650622298E-4
84-85	0.0	0.0	0.0	2.8179620399345215	9.18351650622298E-4
86-87	0.0	0.0	0.0	2.98716833156168	9.18351650622298E-4
88-89	0.0	0.0	0.0	3.1655581396950616	0.0011479395632778724
90-91	0.0	0.0	0.0	3.3592155440200386	0.001377527475933447
92-93	0.0	0.0	0.0	3.5650411077157607	0.001377527475933447
94-95	0.0	0.0	0.0	3.7655861494204053	0.001377527475933447
96-97	0.0	0.0	0.0	3.962228196609905	0.001377527475933447
98-99	0.0	0.0	0.0	4.178959186156767	0.001377527475933447
100-101	0.0	0.0	0.0	4.409924626288275	0.001377527475933447
102-103	0.0	0.0	0.0	4.661438184602457	0.001377527475933447
104-105	0.0	0.0	0.0	4.906293693449627	0.001377527475933447
106-107	0.0	0.0	0.0	5.151608378122109	0.001377527475933447
108-109	0.0	2.295879126555745E-4	0.0	5.420800205710769	0.001377527475933447
110-111	0.0	2.295879126555745E-4	0.0	5.696994464635425	0.0014923214322612342
112-113	0.0	2.295879126555745E-4	0.0	5.992588902179478	0.0016071153885890215
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAACG	165	0.0	61.298355	1
GCGCAGA	305	0.0	60.470802	1
TCTAACG	65	3.5253186E-5	45.74998	2
GGTATCA	2120	0.0	38.1669	1
GTATCAA	3000	0.0	35.49896	1
CTAACGC	70	0.0027928478	33.9857	3
ATCAACG	3525	0.0	29.864029	3
CAACGCA	3685	0.0	28.89015	5
TCAACGC	3745	0.0	28.586103	4
AACGCAG	3800	0.0	28.485382	6
TATCAAC	3725	0.0	28.26059	2
GTGTTAT	155	6.8226163E-6	26.86892	1
AGTACTA	90	0.009542136	26.433321	5
ACGCAGA	4115	0.0	26.160316	7
CGCAGAG	4395	0.0	24.35835	8
GCAGAGT	4670	0.0	22.923973	9
GAGTACT	1925	0.0	22.554146	12-13
GCGGTAT	185	2.6871116E-5	22.511797	1
TAACGCC	215	3.4267432E-6	22.130224	4
GTCTAAC	140	0.003153945	21.24838	1
>>END_MODULE