FastQCFastQC Report
Sun 1 Jan 2017
ERR1142641_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142641_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414950
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9380.22605133148572115No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7960.19183034100494037No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4390.10579587902156885No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT953.2691192E-431.3379551
TATCGGC800.00534293929.767462
TTATGTA1254.855702E-528.5767632
TAACGCA850.007184514328.0164344
TAGTAAC850.007184514328.0164344
GTATCAA23650.025.4281351
ATGGGTA2901.4370016E-1024.6381155
TAGGGGA1250.001626203523.8139694
CATGGGT7350.023.489974
CAACGCA27850.021.3795765
TATCAAC28850.021.2551022
GGTATCA18550.021.1847951
ATCAACG28000.021.0498473
TCAACGC28250.020.6528224
AACGCAG27900.020.4925636
GAGTAAA1805.747587E-419.8473721
TATGTAC1500.004684973519.8449733
TGGGGGG5850.019.343146
GTACATA1550.005658963719.2071341
CATGGGG16200.018.7424764
Produced by FastQC (version 0.11.5)