FastQCFastQC Report
Sun 1 Jan 2017
ERR1142641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414950
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19800.4771659236052536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16740.4034220990480781No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.15809133630557898No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6150.1482106277864803No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.12097843113628148No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAGA2900.055.4209181
TCTAACG851.7114538E-434.9900782
GGTATCA22600.031.0800361
GTCAACG1203.679323E-529.7630841
GTATCAA30250.029.713891
GTATTAC1055.884076E-428.3457971
CTAACGC850.007215122727.9920643
GCTTAGT900.00954085326.4337628
CAACGCA36050.025.7402735
TCAACGC36550.025.3881474
ATCAACG35350.025.0720963
AACGCAG38100.024.6645746
GCACCGT1451.3417356E-424.6107436
TATCAAC36750.024.1169682
TAACAGT1250.00163561723.7903846
ACGCAGA40150.023.1089677
CGCAGAG42100.021.8973268
GTCCTAG1400.003144271421.2593461
GTCCTAC1400.003144271421.2593461
TAGTACT1400.003157492721.2439754
Produced by FastQC (version 0.11.5)