##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142641_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	414950
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.548025063260635	27.0	14.0	33.0	14.0	33.0
2	25.864227015303047	27.0	27.0	33.0	14.0	33.0
3	25.28885166887577	27.0	14.0	33.0	14.0	33.0
4	30.359142065309072	33.0	27.0	33.0	27.0	33.0
5	31.96823472707555	33.0	33.0	33.0	33.0	33.0
6	35.64238823954693	37.0	37.0	37.0	33.0	37.0
7	35.70588022653332	37.0	37.0	37.0	33.0	37.0
8	36.02335462103868	37.0	37.0	37.0	33.0	37.0
9	36.18129654175202	37.0	37.0	37.0	37.0	37.0
10-11	36.163861911073624	37.0	37.0	37.0	37.0	37.0
12-13	36.21256777925051	37.0	37.0	37.0	37.0	37.0
14-15	36.207696107964814	37.0	37.0	37.0	37.0	37.0
16-17	36.197277985299436	37.0	37.0	37.0	37.0	37.0
18-19	36.19327870827811	37.0	37.0	37.0	37.0	37.0
20-21	36.20329919267382	37.0	37.0	37.0	37.0	37.0
22-23	36.199618026268226	37.0	37.0	37.0	37.0	37.0
24-25	36.17987468369683	37.0	37.0	37.0	37.0	37.0
26-27	36.068013013616095	37.0	37.0	37.0	37.0	37.0
28-29	36.03006024822268	37.0	37.0	37.0	37.0	37.0
30-31	36.050927822629234	37.0	37.0	37.0	37.0	37.0
32-33	36.00395228340764	37.0	37.0	37.0	37.0	37.0
34-35	35.91722255693457	37.0	37.0	37.0	37.0	37.0
36-37	35.840131341125435	37.0	37.0	37.0	33.0	37.0
38-39	35.76819014339077	37.0	37.0	37.0	33.0	37.0
40-41	35.7049752982287	37.0	37.0	37.0	33.0	37.0
42-43	35.6270815760935	37.0	37.0	37.0	33.0	37.0
44-45	35.53646463429329	37.0	37.0	37.0	33.0	37.0
46-47	35.41222195445235	37.0	37.0	37.0	33.0	37.0
48-49	35.28345945294613	37.0	37.0	37.0	33.0	37.0
50-51	35.2120026509218	37.0	37.0	37.0	33.0	37.0
52-53	35.149449331244725	37.0	37.0	37.0	33.0	37.0
54-55	35.08090733823352	37.0	37.0	37.0	33.0	37.0
56-57	35.04494396915291	37.0	37.0	37.0	33.0	37.0
58-59	35.00031088082902	37.0	37.0	37.0	33.0	37.0
60-61	34.97431618267261	37.0	37.0	37.0	27.0	37.0
62-63	34.95368237137004	37.0	37.0	37.0	27.0	37.0
64-65	34.94643812507531	37.0	37.0	37.0	27.0	37.0
66-67	34.91761537534643	37.0	37.0	37.0	27.0	37.0
68-69	34.88068682973852	37.0	37.0	37.0	27.0	37.0
70-71	34.830555488613086	37.0	37.0	37.0	27.0	37.0
72-73	34.80627665983853	37.0	37.0	37.0	27.0	37.0
74-75	34.719001084468005	37.0	37.0	37.0	27.0	37.0
76-77	34.66149897578022	37.0	37.0	37.0	27.0	37.0
78-79	34.63566935775395	37.0	37.0	37.0	27.0	37.0
80-81	34.61052415953729	37.0	37.0	37.0	27.0	37.0
82-83	34.57719966260996	37.0	37.0	37.0	27.0	37.0
84-85	34.47337992529221	37.0	37.0	37.0	27.0	37.0
86-87	34.35493553440173	37.0	37.0	37.0	27.0	37.0
88-89	34.2947186408001	37.0	37.0	37.0	27.0	37.0
90-91	34.26206530907338	37.0	37.0	37.0	27.0	37.0
92-93	34.15677431015785	37.0	37.0	37.0	27.0	37.0
94-95	34.19844318592602	37.0	37.0	37.0	27.0	37.0
96-97	34.18133269068562	37.0	37.0	37.0	27.0	37.0
98-99	34.13506687552717	37.0	37.0	37.0	27.0	37.0
100-101	34.040112061694174	37.0	37.0	37.0	24.5	37.0
102-103	33.95623569104711	37.0	37.0	37.0	22.0	37.0
104-105	33.86502590673575	37.0	37.0	37.0	22.0	37.0
106-107	33.76728039522834	37.0	35.0	37.0	22.0	37.0
108-109	33.6647367152669	37.0	33.0	37.0	22.0	37.0
110-111	33.616187492468974	37.0	33.0	37.0	22.0	37.0
112-113	33.55663694421014	37.0	33.0	37.0	22.0	37.0
114-115	33.45533678756476	37.0	33.0	37.0	22.0	37.0
116-117	33.35423183516086	37.0	33.0	37.0	18.0	37.0
118-119	33.247262320761536	37.0	33.0	37.0	14.0	37.0
120-121	33.183842631642364	37.0	33.0	37.0	14.0	37.0
122-123	33.07696107964815	37.0	33.0	37.0	14.0	37.0
124-125	31.814374020966383	37.0	30.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	137.0
16	84.0
17	19.0
18	61.0
19	283.0
20	1017.0
21	2418.0
22	3949.0
23	5049.0
24	4237.0
25	3177.0
26	3046.0
27	3669.0
28	4597.0
29	5896.0
30	7879.0
31	10533.0
32	14919.0
33	22472.0
34	36998.0
35	83340.0
36	201166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	54.53344423148785	11.93167709350402	11.659735532009789	21.87514314299835
2	18.73526930955537	19.614411374864442	35.60187974454754	26.048439571032656
3	22.397879262561755	24.37546692372575	28.275454874081213	24.95119893963128
4	15.344480219017505	17.062061386596746	34.879164417367164	32.714293977018585
5	16.313472008329178	34.864205028836395	31.639059400810265	17.183263562024162
6	30.41947424737558	33.582280189324926	18.622574394863936	17.375671168435563
7	26.62682250873599	31.46547776840583	22.609230027714183	19.298469695143993
8	26.10531389324015	31.14375225930835	21.47584046270635	21.27509338474515
9	27.10832630437402	15.77250271117002	21.02132787082781	36.097843113628144
10-11	25.743101578503435	24.639715628388963	26.568261236293527	23.048921556814072
12-13	26.76406795999518	23.686347752741295	26.62079768646825	22.928786600795277
14-15	25.099891553199182	23.819978310639836	25.447764790938663	25.632365345222315
16-17	23.845523557055067	25.978551632726838	26.27485239185444	23.901072418363658
18-19	23.755994698156403	24.65453669116761	28.569827690083144	23.019640920592842
20-21	25.031449723820813	23.703211004752404	28.34126203765291	22.924077233773872
22-23	24.737438245571756	23.590553078684177	28.297746716471867	23.374261959272204
24-25	23.956259790336183	24.274490902518377	28.33485962164116	23.43438968550428
26-27	23.630075912760574	24.310880829015545	28.408482949752983	23.6505603084709
28-29	23.554042655741654	24.205084949993974	28.812387034582482	23.42848535968189
30-31	23.957826244125798	24.054584889745755	28.708157609350526	23.279431256777926
32-33	23.532594288468488	24.10362694300518	28.467767200867574	23.896011567658753
34-35	23.729605976623688	24.2875045186167	28.435112664176405	23.547776840583204
36-37	23.838225122726517	24.37058226713291	28.067680932745624	23.723511677394946
38-39	23.752557543216156	24.170801712981596	28.04127736179627	24.035363382005983
40-41	23.586351663023546	24.375387106958403	28.20271511096865	23.835546119049397
42-43	23.93507612408799	24.189686647105958	28.08692665939667	23.788310569409383
44-45	23.797565971803834	24.356789974695747	27.760332570189178	24.08531148331124
46-47	23.784431859260152	24.349319195083748	27.614290878419084	24.251958067237016
48-49	23.870707314134233	24.61163995662128	27.503193155801902	24.014459573442583
50-51	23.71972526810459	24.96830943487167	27.192673816122426	24.119291480901314
52-53	23.744065550066274	24.730449451741173	27.10929027593686	24.416194722255693
54-55	23.727465631359962	24.784823213427178	27.18836870510749	24.299342450105375
56-57	23.876973129292686	24.551994216170623	26.810097602120734	24.760935052415952
58-59	23.81090092337205	24.586543084469923	27.14181225772479	24.460743734433237
60-61	23.8537173153392	24.60248222677431	27.410169899987952	24.13363055789854
62-63	23.40053018435956	24.68719122785878	27.503072659356548	24.409205928425113
64-65	23.469092661766478	24.419086636944208	27.509338474514998	24.60248222677431
66-67	23.66092300277142	24.557778045547657	27.395589830100015	24.385709121580916
68-69	23.81732738884203	24.36582720809736	27.433305217496084	24.383540185564527
70-71	23.401458253253114	24.320246970407172	27.88117079589395	24.397123980445766
72-73	23.666947825039163	24.46644173996867	27.709362573804075	24.157247861188093
74-75	23.70244405448921	24.477932352817025	27.709173797420267	24.1104497952735
76-77	23.543324275657284	24.517235515256335	27.75785732290796	24.181582886178422
78-79	23.534061537905398	24.470565553473634	27.707601564053284	24.287771344567684
80-81	23.24810218098566	24.653211230268706	27.84190866369442	24.256777925051214
82-83	23.551994216170623	24.657428605856126	27.6815278949271	24.10904928304615
84-85	23.519548961968397	24.68827689527768	27.408835240603075	24.38333890215085
86-87	23.25514856875885	24.699608975573952	27.56848963646439	24.47675281920281
88-89	23.572764033218835	24.356976873390632	27.420153533245404	24.65010556014513
90-91	23.756455001314517	24.37536330110154	27.406108523616684	24.462073173967262
92-93	23.454544796266504	24.756006164562937	27.234020470500493	24.555428568670067
94-95	22.934505204254016	24.786419535894773	27.456265709761922	24.82280955008929
96-97	23.303197136037035	24.62287579572936	27.18354884148553	24.890378226748076
98-99	23.010965176527293	24.71695384986143	27.09965056030847	25.17243041330281
100-101	23.25207856368237	25.030365104229425	26.733220869984336	24.984335462103868
102-103	23.509728292237973	24.768676670293612	26.93159047064763	24.79000456682078
104-105	23.339398467248277	24.903841519255796	26.829662119824555	24.927097893671373
106-107	23.30601557124489	24.830604569669934	26.92504666432893	24.938333194756243
108-109	23.42727960705489	24.89600354768894	26.672619650147738	25.004097195108432
110-111	23.23489753350179	25.018767751039007	26.770705679488987	24.97562903597022
112-113	23.081984122776152	25.23413362125061	26.584589356379567	25.09929289959367
114-115	23.145895466184665	25.25476728422955	26.589426118391323	25.009911131194457
116-117	23.12428756304992	25.270454923376125	26.53362220417449	25.071635309399472
118-119	22.954570923511273	25.094982810912814	26.783164454919767	25.16728181065615
120-121	22.893842631642368	25.090974816242923	26.921074828292564	25.094107723822145
122-123	22.984214965658513	25.235932040004823	26.748282925653694	25.031570068682974
124-125	22.8574527051452	25.20219303530546	26.758404627063502	25.18194963248584
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	142.0
1	124.0
2	214.0
3	321.0
4	256.0
5	233.5
6	321.0
7	532.0
8	882.0
9	1055.0
10	965.0
11	828.0
12	739.5
13	699.0
14	717.0
15	724.5
16	680.0
17	679.5
18	703.5
19	692.0
20	686.5
21	685.5
22	721.5
23	772.5
24	808.0
25	921.0
26	1060.5
27	1228.0
28	1489.0
29	1835.5
30	2336.0
31	2853.0
32	3458.0
33	4076.0
34	4655.0
35	5336.5
36	6011.5
37	6868.5
38	7690.5
39	8270.0
40	8604.0
41	8909.0
42	9525.0
43	10503.5
44	11597.5
45	12619.0
46	13825.0
47	15013.5
48	16250.5
49	17670.0
50	18666.5
51	18156.5
52	17057.5
53	16908.5
54	16766.0
55	16364.5
56	15730.0
57	14514.0
58	13556.5
59	12410.0
60	10862.0
61	9201.0
62	7457.0
63	5862.0
64	4517.0
65	3583.5
66	2783.5
67	2089.5
68	1729.0
69	1493.0
70	1246.0
71	1107.5
72	1017.0
73	990.0
74	794.0
75	630.0
76	523.5
77	366.5
78	263.5
79	171.5
80	112.0
81	90.5
82	78.0
83	58.5
84	30.5
85	17.5
86	9.5
87	5.0
88	4.0
89	2.5
90	2.5
91	2.0
92	1.0
93	0.5
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03735389806000723
2	0.0
3	0.0
4	0.0014459573442583444
5	0.005542836486323653
6	4.819857814194481E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	4.819857814194481E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	7.229786721291722E-4
38-39	2.4099289070972406E-4
40-41	0.0031329075792264124
42-43	6.024822267743102E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.2049644535486203E-4
56-57	0.0
58-59	8.434751174840342E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.614893360645861E-4
72-73	0.0
74-75	0.0897698517893722
76-77	0.0926617664778889
78-79	6.024822267743102E-4
80-81	0.0
82-83	0.0
84-85	0.10591637546692372
86-87	0.18833594408964935
88-89	0.23328111820701292
90-91	0.0841065188576937
92-93	0.1558019038438366
94-95	2.4099289070972406E-4
96-97	1.2049644535486203E-4
98-99	0.0
100-101	0.0
102-103	1.2049644535486203E-4
104-105	0.0024099289070972406
106-107	0.004458368478129895
108-109	0.00771177250271117
110-111	0.002289432461742379
112-113	0.003855886251355585
114-115	0.0030124111338715506
116-117	2.4099289070972406E-4
118-119	0.0015664537896132062
120-121	0.0
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	414950.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.9046435782125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.371922563003	68.84583129592143
2	8.218116023595027	12.804587994051348
3	1.6979027054489333	3.9682351529544557
4	0.6317575677264994	1.968673925662855
5	0.3032162041526272	1.1810975155824468
6	0.17551280080710496	0.8203957314174791
7	0.11389580619485382	0.6211108530664277
8	0.077101273272336	0.48052377709661576
9	0.05482520202236756	0.3844024040390436
>10	0.3141972594134121	4.480895849842866
>50	0.030906888375241713	1.6667258293051084
>100	0.008928656635561477	1.3256222279042842
>500	0.001030229611795555	0.47433465389995877
>1k	6.868197411970367E-4	0.9775627892557527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1980	0.4771659236052536	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1674	0.4034220990480781	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	656	0.15809133630557898	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	615	0.1482106277864803	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	502	0.12097843113628148	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	2.4099289070972406E-4	0.0	0.0	0.0	0.0
7	2.4099289070972406E-4	0.0	0.0	0.0	0.0
8	2.4099289070972406E-4	0.0	0.0	0.0	0.0
9	2.4099289070972406E-4	0.0	0.0	0.0	0.0
10-11	2.4099289070972406E-4	0.0	0.0	0.0	0.0
12-13	2.4099289070972406E-4	0.0	0.0	0.0	0.0
14-15	2.4099289070972406E-4	0.0	0.0	1.2049644535486203E-4	0.0
16-17	2.4099289070972406E-4	0.0	0.0	4.819857814194481E-4	4.819857814194481E-4
18-19	2.4099289070972406E-4	0.0	0.0	0.0012049644535486203	4.819857814194481E-4
20-21	2.4099289070972406E-4	0.0	0.0	0.0019279431256777925	4.819857814194481E-4
22-23	2.4099289070972406E-4	0.0	0.0	0.0036148933606458614	4.819857814194481E-4
24-25	2.4099289070972406E-4	0.0	0.0	0.006988793830581997	4.819857814194481E-4
26-27	2.4099289070972406E-4	0.0	0.0	0.012049644535486204	4.819857814194481E-4
28-29	2.4099289070972406E-4	0.0	0.0	0.02072538860103627	4.819857814194481E-4
30-31	2.4099289070972406E-4	0.0	0.0	0.040486805639233646	4.819857814194481E-4
32-33	2.4099289070972406E-4	0.0	0.0	0.07663573924569225	4.819857814194481E-4
34-35	2.4099289070972406E-4	0.0	0.0	0.11182070128931196	4.819857814194481E-4
36-37	2.4099289070972406E-4	0.0	0.0	0.16327268345583806	4.819857814194481E-4
38-39	2.4099289070972406E-4	0.0	0.0	0.23930594047475598	4.819857814194481E-4
40-41	2.4099289070972406E-4	0.0	0.0	0.3250994095674178	4.819857814194481E-4
42-43	2.4099289070972406E-4	0.0	0.0	0.3987227376792385	4.819857814194481E-4
44-45	2.4099289070972406E-4	0.0	0.0	0.4805398240751898	4.819857814194481E-4
46-47	2.4099289070972406E-4	0.0	0.0	0.5813953488372093	4.819857814194481E-4
48-49	2.4099289070972406E-4	0.0	0.0	0.6765875406675503	4.819857814194481E-4
50-51	2.4099289070972406E-4	0.0	0.0	0.7634654777684058	4.819857814194481E-4
52-53	2.4099289070972406E-4	0.0	0.0	0.8467285215086156	4.819857814194481E-4
54-55	2.4099289070972406E-4	0.0	0.0	0.937944330642246	4.819857814194481E-4
56-57	2.4099289070972406E-4	0.0	0.0	1.0421737558742017	4.819857814194481E-4
58-59	2.4099289070972406E-4	0.0	0.0	1.1579708398602242	6.024822267743102E-4
60-61	2.4099289070972406E-4	0.0	0.0	1.274972888299795	7.229786721291722E-4
62-63	2.4099289070972406E-4	0.0	0.0	1.3841426677913002	7.229786721291722E-4
64-65	2.4099289070972406E-4	0.0	0.0	1.4848776961079648	7.229786721291722E-4
66-67	2.4099289070972406E-4	0.0	0.0	1.598144354741535	7.229786721291722E-4
68-69	2.4099289070972406E-4	0.0	0.0	1.7314134233040126	7.229786721291722E-4
70-71	2.4099289070972406E-4	0.0	0.0	1.8639595131943607	7.229786721291722E-4
72-73	2.4099289070972406E-4	0.0	0.0	1.9932521990601277	7.229786721291722E-4
74-75	2.4099289070972406E-4	0.0	0.0	2.1130256657428603	7.229786721291722E-4
76-77	2.4099289070972406E-4	0.0	0.0	2.2589468610675985	7.229786721291722E-4
78-79	2.4099289070972406E-4	0.0	0.0	2.418243161826726	7.229786721291722E-4
80-81	2.4099289070972406E-4	0.0	0.0	2.587058681768888	7.229786721291722E-4
82-83	2.4099289070972406E-4	0.0	0.0	2.7609350524159537	7.229786721291722E-4
84-85	2.4099289070972406E-4	0.0	0.0	2.9220387998554043	9.639715628388962E-4
86-87	2.4099289070972406E-4	0.0	0.0	3.0904928304615016	0.0010844680081937583
88-89	2.4099289070972406E-4	0.0	0.0	3.274129413182311	0.0012049644535486203
90-91	2.4099289070972406E-4	0.0	0.0	3.476804434269189	0.0012049644535486203
92-93	2.4099289070972406E-4	0.0	0.0	3.682732859380648	0.0012049644535486203
94-95	2.4099289070972406E-4	0.0	0.0	3.859380648270876	0.0012049644535486203
96-97	2.4099289070972406E-4	0.0	0.0	4.054102903964333	0.0016869502349680684
98-99	2.4099289070972406E-4	0.0	0.0	4.282082178575732	0.0016869502349680684
100-101	2.4099289070972406E-4	0.0	0.0	4.523436558621521	0.0016869502349680684
102-103	2.4099289070972406E-4	0.0	0.0	4.761537534642728	0.0016869502349680684
104-105	2.4099289070972406E-4	0.0	0.0	5.010362694300518	0.0016869502349680684
106-107	2.4099289070972406E-4	0.0	0.0	5.273526930955537	0.0016869502349680684
108-109	2.4099289070972406E-4	0.0	0.0	5.547294854801783	0.0016869502349680684
110-111	2.4099289070972406E-4	0.0	0.0	5.834317387637064	0.0016869502349680684
112-113	2.4099289070972406E-4	0.0	0.0	6.147005663332932	0.0016869502349680684
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCAGA	290	0.0	55.420918	1
TCTAACG	85	1.7114538E-4	34.990078	2
GGTATCA	2260	0.0	31.080036	1
GTCAACG	120	3.679323E-5	29.763084	1
GTATCAA	3025	0.0	29.71389	1
GTATTAC	105	5.884076E-4	28.345797	1
CTAACGC	85	0.0072151227	27.992064	3
GCTTAGT	90	0.009540853	26.433762	8
CAACGCA	3605	0.0	25.740273	5
TCAACGC	3655	0.0	25.388147	4
ATCAACG	3535	0.0	25.072096	3
AACGCAG	3810	0.0	24.664574	6
GCACCGT	145	1.3417356E-4	24.610743	6
TATCAAC	3675	0.0	24.116968	2
TAACAGT	125	0.001635617	23.790384	6
ACGCAGA	4015	0.0	23.108967	7
CGCAGAG	4210	0.0	21.897326	8
GTCCTAG	140	0.0031442714	21.259346	1
GTCCTAC	140	0.0031442714	21.259346	1
TAGTACT	140	0.0031574927	21.243975	4
>>END_MODULE