FastQCFastQC Report
Sun 1 Jan 2017
ERR1142640_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142640_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387851
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7700.19852984780237773No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7140.18409131341675025No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATG1004.403701E-429.783987
CCTTACG800.00533140729.7801251
GTATACT900.00946844726.4746483
ATGGGTA3301.8189894E-1225.2712575
GGTATCA13500.025.1476611
CATGGGT5400.023.1653184
TATACTG1300.00203615122.9107535
TCTATAT1300.00203615122.9107533
GTATCAA17600.021.9966831
TGGGTAT1450.003837341420.5406766
GTACATG28550.019.610031
AACGCAG19200.019.5457366
ATCAACG19450.018.9882453
TATCAAC21050.018.9549662
ACATGGG28000.018.721363
CAACGCA20050.018.7171155
GTTACTG1600.00677983418.6149883
TAACGTC800.0068417918.5885246-47
TACATGG29000.018.4818252
GTTCTAC1959.825131E-418.3262311
Produced by FastQC (version 0.11.5)