##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1142640_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 387851 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89652211802986 33.0 33.0 33.0 27.0 33.0 2 31.07801191694749 33.0 33.0 33.0 27.0 33.0 3 31.13993775960356 33.0 33.0 33.0 27.0 33.0 4 31.10553537312009 33.0 33.0 33.0 27.0 33.0 5 31.12963741230524 33.0 33.0 33.0 27.0 33.0 6 34.29749568777701 37.0 37.0 37.0 27.0 37.0 7 34.30016939494806 37.0 37.0 37.0 27.0 37.0 8 34.265264238070806 37.0 37.0 37.0 27.0 37.0 9 34.23061433385501 37.0 37.0 37.0 27.0 37.0 10-11 34.30857597376312 37.0 37.0 37.0 27.0 37.0 12-13 34.28497025919748 37.0 37.0 37.0 27.0 37.0 14-15 34.28145731221526 37.0 37.0 37.0 27.0 37.0 16-17 34.29507207664799 37.0 37.0 37.0 27.0 37.0 18-19 34.24738237106518 37.0 37.0 37.0 27.0 37.0 20-21 34.27738358287074 37.0 37.0 37.0 27.0 37.0 22-23 34.35262510603299 37.0 37.0 37.0 27.0 37.0 24-25 34.402639157820914 37.0 37.0 37.0 27.0 37.0 26-27 34.47253326664106 37.0 37.0 37.0 27.0 37.0 28-29 34.48202015722532 37.0 37.0 37.0 27.0 37.0 30-31 34.502128394667025 37.0 37.0 37.0 27.0 37.0 32-33 34.48503677958804 37.0 37.0 37.0 27.0 37.0 34-35 34.48541965858023 37.0 37.0 37.0 27.0 37.0 36-37 34.4581656357725 37.0 37.0 37.0 27.0 37.0 38-39 34.42631190843906 37.0 37.0 37.0 27.0 37.0 40-41 34.38872144199705 37.0 37.0 37.0 27.0 37.0 42-43 34.37438990746446 37.0 37.0 37.0 27.0 37.0 44-45 34.395926528486456 37.0 37.0 37.0 27.0 37.0 46-47 34.382489667423826 37.0 37.0 37.0 27.0 37.0 48-49 34.33513772041325 37.0 37.0 37.0 27.0 37.0 50-51 34.315536120829904 37.0 37.0 37.0 27.0 37.0 52-53 34.26655726039124 37.0 37.0 37.0 27.0 37.0 54-55 34.23014765979719 37.0 37.0 37.0 27.0 37.0 56-57 34.1885994879477 37.0 37.0 37.0 27.0 37.0 58-59 34.146475063877624 37.0 37.0 37.0 27.0 37.0 60-61 34.12675228373783 37.0 37.0 37.0 27.0 37.0 62-63 34.06978066319283 37.0 37.0 37.0 27.0 37.0 64-65 34.01730948224963 37.0 37.0 37.0 27.0 37.0 66-67 33.959145393462954 37.0 37.0 37.0 27.0 37.0 68-69 33.934993077238424 37.0 37.0 37.0 27.0 37.0 70-71 33.87475473828867 37.0 37.0 37.0 27.0 37.0 72-73 33.81961371763899 37.0 37.0 37.0 27.0 37.0 74-75 33.752733652871854 37.0 37.0 37.0 24.5 37.0 76-77 33.68496407125417 37.0 33.0 37.0 22.0 37.0 78-79 33.62360030011525 37.0 33.0 37.0 22.0 37.0 80-81 33.55441006984641 37.0 33.0 37.0 22.0 37.0 82-83 33.51294311475283 37.0 33.0 37.0 22.0 37.0 84-85 33.44313280099833 37.0 33.0 37.0 14.0 37.0 86-87 33.3580073275562 37.0 33.0 37.0 14.0 37.0 88-89 33.28366434532849 37.0 33.0 37.0 14.0 37.0 90-91 33.19982544843252 37.0 33.0 37.0 14.0 37.0 92-93 33.115826696334416 37.0 33.0 37.0 14.0 37.0 94-95 33.06561927131811 37.0 33.0 37.0 14.0 37.0 96-97 32.93599861802599 37.0 33.0 37.0 14.0 37.0 98-99 32.82659320202862 37.0 33.0 37.0 14.0 37.0 100-101 32.74372632789396 37.0 33.0 37.0 14.0 37.0 102-103 32.67180953510497 37.0 33.0 37.0 14.0 37.0 104-105 32.47578193687782 37.0 33.0 37.0 14.0 37.0 106-107 32.38168781310348 37.0 33.0 37.0 14.0 37.0 108-109 32.27307135987789 37.0 33.0 37.0 14.0 37.0 110-111 32.15441883609943 37.0 33.0 37.0 14.0 37.0 112-113 32.038862862284745 37.0 33.0 37.0 14.0 37.0 114-115 31.895440517105797 37.0 30.0 37.0 14.0 37.0 116-117 31.795103016364532 37.0 27.0 37.0 14.0 37.0 118-119 31.660771791229106 37.0 27.0 37.0 14.0 37.0 120-121 31.514298016506338 37.0 27.0 37.0 14.0 37.0 122-123 31.378406913995324 37.0 27.0 37.0 14.0 37.0 124-125 29.810995717427566 35.0 24.5 37.0 14.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 743.0 1 33.0 2 19.0 3 15.0 4 4.0 5 14.0 6 14.0 7 10.0 8 11.0 9 10.0 10 9.0 11 12.0 12 17.0 13 15.0 14 37.0 15 99.0 16 197.0 17 475.0 18 1175.0 19 2429.0 20 3531.0 21 4315.0 22 4537.0 23 4753.0 24 5287.0 25 5695.0 26 6506.0 27 7781.0 28 8980.0 29 10573.0 30 12583.0 31 15195.0 32 18325.0 33 24287.0 34 34654.0 35 61052.0 36 154459.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.05883098507756 19.29673899829022 11.990977731447831 24.65345228518439 2 17.74974271987754 22.429396645791208 35.47647244387673 24.34438819045452 3 19.835918067628853 26.850640452169056 27.872985174191612 25.44045630601048 4 14.816615295504851 18.636871392813553 33.63811257524669 32.90840073643491 5 15.902161235346297 36.928495876403304 30.871761185692453 16.29758170255795 6 30.44559258406443 35.321420163045026 18.005065727687384 16.227921525203158 7 26.89885372732683 32.580536652262786 21.31239164747587 19.20821797293451 8 25.778272288564295 33.373526710051365 20.347508830036354 20.500692171347985 9 26.463350575352106 18.236863418887904 19.638046985579326 35.66173902018067 10-11 25.26364136235728 26.777501051201604 25.587476145809752 22.371381440631367 12-13 25.88270457991957 25.631945055456928 25.146082302950628 23.33926806167287 14-15 24.630010337328105 26.0459267175296 23.035810626359282 26.288252318783016 16-17 24.35000620642572 27.737680244946933 23.926546951610568 23.985766597016777 18-19 24.431653634520707 26.87712030946175 25.18113131438501 23.510094741632535 20-21 25.944305342090534 25.887170683974247 24.56893710146314 23.59958687247208 22-23 25.547181078709023 25.639988263381102 24.577875956347018 24.23495470156286 24-25 24.814240865846298 26.313626300055446 24.565441886374476 24.30669094772378 26-27 24.528097032866473 26.338803323355 24.69612602691566 24.436973616862872 28-29 24.354852050205743 26.37481648435723 25.031533673828083 24.238797791608942 30-31 24.80304173860899 26.075257699982423 25.044845483400707 24.076855078007878 32-33 24.290252621729138 26.264982694132033 24.73304503144524 24.71171965269359 34-35 24.663951830834108 26.220868394218975 24.90304003887445 24.212139736072466 36-37 24.53364176191984 26.49690171423402 24.537389427705588 24.43206709614055 38-39 24.509577122947228 26.381448774895933 24.52327626649228 24.585697835664558 40-41 24.17525620003437 26.533679111314406 24.90441357940941 24.386651109241807 42-43 24.496441784703098 26.45510781902789 24.82273536104869 24.225715035220322 44-45 24.31722478703047 26.568700036307863 24.753048374738512 24.361026801923156 46-47 24.122808717708182 26.274971928586844 24.931937321606007 24.670282032098967 48-49 24.249179107659984 26.72923668965553 24.794243868021447 24.227340334663044 50-51 24.07906605292608 26.72782534755516 24.749106397496103 24.444002202022656 52-53 24.076462423980832 26.362917378505024 24.822245024023736 24.738375173490407 54-55 24.125044581246414 26.189867005742585 24.97544800922121 24.70964040378979 56-57 24.213330990210572 26.06086605952221 24.705128337657772 25.020674612609444 58-59 24.232622632414838 26.14064719055822 24.934708472197634 24.692021704829312 60-61 24.10105317568004 26.29243393422498 25.033533630548554 24.572979259546422 62-63 23.8342738918446 26.374363394591327 24.821569567515386 24.969793146048687 64-65 23.836878699371912 25.90503760758872 25.124841686267413 25.13324200677195 66-67 24.207552023080297 25.85171273203412 24.93527409787668 25.005461147008905 68-69 24.212031050875147 25.73756168427474 24.771939438902518 25.278467825947594 70-71 23.886982428249222 25.77470159044055 25.123015180596752 25.215300800713468 72-73 24.236204636927397 25.81523635715043 25.102817363791225 24.84574164213095 74-75 24.162873000731587 25.645310977201813 25.28662535642364 24.90519066564296 76-77 24.211180734352865 25.508215197857538 25.11206067562118 25.16854339216842 78-79 24.196479346533632 25.53507728894174 25.242852711575246 25.025590652949386 80-81 24.016812001039117 25.64967501709246 25.38020221445899 24.953310767409427 82-83 24.17156792425764 25.642655996981055 25.349420572487563 24.836355506273737 84-85 24.1163510431406 25.688459873492146 25.08008502775032 25.11510405561694 86-87 23.886800790218654 25.791672211720716 25.148747420742335 25.172779577318295 88-89 23.99418623429476 25.272310325893866 25.296728141855883 25.436775297955492 90-91 24.343750969062363 25.356098115625937 25.29226920810808 25.007881707203623 92-93 24.21104855026214 25.77822796323524 25.081330539197573 24.929392947305047 94-95 23.604470448711407 25.890336808081017 25.340509339703203 25.164683403504373 96-97 23.855651086608315 25.73848546538664 25.21068213994151 25.195181308063532 98-99 23.651400822394557 25.978805835565012 25.06029730738773 25.309496034652707 100-101 23.807918231413264 26.22457165667425 24.846690359094005 25.12081975281848 102-103 24.0210928003121 26.05841858254005 25.206981499877923 24.713507117269923 104-105 23.829862551079863 26.20650540739219 25.166756456120087 24.796875585407857 106-107 23.72841943168371 26.337078013011645 25.093143308925736 24.841359246378907 108-109 23.900398962284626 26.331898378311337 24.930362476873626 24.83734018253041 110-111 23.623386857165002 26.77888155430107 24.728526953533738 24.869204635000195 112-113 23.693304172926553 26.709416890902958 24.78216891936407 24.81511001680642 114-115 23.599617625402075 26.790378628360312 24.91015488754828 24.699848858689332 116-117 23.743019285696754 26.919623768080747 24.692125147106886 24.645231799115614 118-119 23.464920496103282 26.612652158018275 24.94726222140695 24.97516512447149 120-121 23.3430828180834 26.581341449865516 25.182479865849842 24.89309586620124 122-123 23.44361193644232 26.71786590879731 25.126856995220255 24.71166515954011 124-125 23.496599817295902 26.677640972233885 24.908615648205455 24.91714356226476 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1127.0 1 674.5 2 180.0 3 117.0 4 84.5 5 70.5 6 66.5 7 73.0 8 84.0 9 70.0 10 58.5 11 68.0 12 72.0 13 75.5 14 81.5 15 82.0 16 87.0 17 103.5 18 121.5 19 144.5 20 176.5 21 211.5 22 286.0 23 412.5 24 517.0 25 657.0 26 860.5 27 1150.0 28 1458.5 29 1878.5 30 2448.0 31 2967.0 32 3672.0 33 4364.5 34 4921.0 35 5529.5 36 6247.0 37 7047.0 38 7653.0 39 8018.0 40 8529.0 41 8990.5 42 9245.5 43 10034.0 44 11266.5 45 12294.0 46 13441.5 47 14557.5 48 15626.0 49 16438.0 50 16715.5 51 16431.5 52 16179.0 53 16402.5 54 16057.0 55 15471.5 56 14881.5 57 13862.5 58 12894.0 59 11700.5 60 10096.0 61 8455.0 62 7022.0 63 5564.5 64 4159.5 65 3355.0 66 2632.5 67 1936.0 68 1627.0 69 1424.5 70 1191.5 71 1092.5 72 1005.0 73 950.0 74 776.5 75 627.0 76 513.5 77 342.0 78 245.5 79 166.5 80 107.0 81 77.0 82 54.5 83 28.0 84 14.5 85 6.5 86 3.0 87 2.5 88 2.0 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3228043759072427 2 0.286965870914346 3 0.2818092514909076 4 0.28954418062606513 5 0.30269356015583304 6 0.34343085360099623 7 0.3560645711884203 8 0.3573537260442799 9 0.3589007118713114 10-11 0.3579983034722097 12-13 0.36740913391998475 14-15 0.3577404725010378 16-17 0.2977947717035666 18-19 0.2874815328566898 20-21 0.26956228036024144 22-23 0.2649213228791469 24-25 0.2558972388881297 26-27 0.2611827737971541 28-29 0.2482912252385581 30-31 0.24932254912324578 32-33 0.2547369995178561 34-35 0.24867797169531597 36-37 0.24326352130070567 38-39 0.2489358026664879 40-41 0.23140329662679743 42-43 0.23965388770429882 44-45 0.22753583205921862 46-47 0.2298563107997659 48-49 0.2383647328484392 50-51 0.24081412707457245 52-53 0.2428767748439478 54-55 0.2364310005646498 56-57 0.23282136696824296 58-59 0.24042738061781457 60-61 0.2387514793051971 62-63 0.24120087353133035 64-65 0.24777556329621425 66-67 0.2649213228791469 68-69 0.2583466331142629 70-71 0.26002253442688045 72-73 0.2578309711719191 74-75 0.26350325253770135 76-77 0.25963578797012254 78-79 0.2555104924313718 80-81 0.2538345911187544 82-83 0.24764664781062828 84-85 0.23681774702140773 86-87 0.22418402943398366 88-89 0.21619126932765417 90-91 0.22663342366011688 92-93 0.21915632549613126 94-95 0.21155031184655962 96-97 0.1998190026582373 98-99 0.20897200213484046 100-101 0.20819850922132468 102-103 0.20613586145194931 104-105 0.21515994544296652 106-107 0.20961657956277022 108-109 0.21838283258261548 110-111 0.2052334530528476 112-113 0.20536236853843357 114-115 0.2052334530528476 116-117 0.2069093543654651 118-119 0.20484670659608972 120-121 0.21193705830331752 122-123 0.20652260790870722 124-125 0.22856715594390628 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 387851.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.16914115448657 #Duplication Level Percentage of deduplicated Percentage of total 1 89.39948913293347 72.56479752570064 2 7.63038856296143 12.387041726611926 3 1.511478300534206 3.680561865840134 4 0.5574516400800911 1.8099148344184381 5 0.24186150687065225 0.9815845395510397 6 0.14275391051994968 0.6952327388009246 7 0.10360246298878113 0.5886526059602193 8 0.06397452481624952 0.41542057880810884 9 0.042267027074133774 0.3087700458084782 >10 0.2752703447187521 4.204188562631253 >50 0.02651341559272218 1.4608259119857223 >100 0.004242146493916059 0.4771859145972847 >500 7.070244156526766E-4 0.4258231492858512 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 770 0.19852984780237773 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 714 0.18409131341675025 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 1.2891548558595955E-4 0.0 0.0 12-13 0.0 0.0 2.578309711719191E-4 0.0 0.0 14-15 0.0 0.0 2.578309711719191E-4 2.578309711719191E-4 0.0 16-17 0.0 0.0 2.578309711719191E-4 6.445774279297978E-4 0.0 18-19 0.0 0.0 2.578309711719191E-4 0.001160239370273636 0.0 20-21 0.0 0.0 2.578309711719191E-4 0.002191563254961312 0.0 22-23 0.0 0.0 2.578309711719191E-4 0.0024493942261332316 0.0 24-25 0.0 0.0 2.578309711719191E-4 0.005285534909024342 0.0 26-27 0.0 0.0 2.578309711719191E-4 0.012375886616252118 0.0 28-29 0.0 0.0 2.578309711719191E-4 0.025138519689262114 0.0 30-31 0.0 0.0 2.578309711719191E-4 0.047183067724461195 0.0 32-33 0.0 0.0 2.578309711719191E-4 0.0774782068371617 0.0 34-35 0.0 0.0 2.578309711719191E-4 0.11318779634447249 0.0 36-37 0.0 0.0 2.578309711719191E-4 0.17016844097346662 0.0 38-39 0.0 0.0 2.578309711719191E-4 0.24622857746918275 0.0 40-41 0.0 0.0 2.578309711719191E-4 0.3362115864081825 0.0 42-43 0.0 0.0 2.578309711719191E-4 0.4084042583363199 0.0 44-45 0.0 0.0 2.578309711719191E-4 0.481370423177973 0.0 46-47 0.0 0.0 2.578309711719191E-4 0.5790883612521303 0.0 48-49 0.0 0.0 2.578309711719191E-4 0.6784822006389052 0.0 50-51 0.0 0.0 2.578309711719191E-4 0.7604724494715754 0.0 52-53 0.0 0.0 2.578309711719191E-4 0.8427205292754176 1.2891548558595955E-4 54-55 0.0 0.0 2.578309711719191E-4 0.9267734258774633 2.578309711719191E-4 56-57 0.0 0.0 2.578309711719191E-4 1.010310660537165 2.578309711719191E-4 58-59 0.0 0.0 2.578309711719191E-4 1.1042900495293295 2.578309711719191E-4 60-61 0.0 0.0 2.578309711719191E-4 1.2057465366854796 2.578309711719191E-4 62-63 0.0 0.0 2.578309711719191E-4 1.3086210941830756 2.578309711719191E-4 64-65 0.0 0.0 2.578309711719191E-4 1.4068546941995765 2.578309711719191E-4 66-67 0.0 0.0 2.578309711719191E-4 1.5065063645575234 2.578309711719191E-4 68-69 0.0 0.0 2.578309711719191E-4 1.6278158364939113 2.578309711719191E-4 70-71 0.0 0.0 2.578309711719191E-4 1.7652397441285443 2.578309711719191E-4 72-73 0.0 0.0 2.578309711719191E-4 1.8895142722334093 2.578309711719191E-4 74-75 0.0 0.0 2.578309711719191E-4 2.005409293775187 2.578309711719191E-4 76-77 0.0 0.0 2.578309711719191E-4 2.1432199478665774 2.578309711719191E-4 78-79 0.0 0.0 2.578309711719191E-4 2.2934064885742202 2.578309711719191E-4 80-81 0.0 0.0 2.578309711719191E-4 2.4425617053971758 2.578309711719191E-4 82-83 0.0 0.0 2.578309711719191E-4 2.6083470198607195 2.578309711719191E-4 84-85 0.0 0.0 2.578309711719191E-4 2.764334757419731 2.578309711719191E-4 86-87 0.0 0.0 2.578309711719191E-4 2.9308935647967904 2.578309711719191E-4 88-89 0.0 0.0 2.578309711719191E-4 3.107765611020727 2.578309711719191E-4 90-91 0.0 0.0 2.578309711719191E-4 3.30036534648615 2.578309711719191E-4 92-93 0.0 0.0 2.578309711719191E-4 3.4791711249938766 2.578309711719191E-4 94-95 0.0 0.0 2.578309711719191E-4 3.6663564100646897 7.734929135157574E-4 96-97 0.0 0.0 2.578309711719191E-4 3.8674645675787866 7.734929135157574E-4 98-99 0.0 0.0 2.578309711719191E-4 4.091777512498357 7.734929135157574E-4 100-101 0.0 0.0 2.578309711719191E-4 4.33220489311617 7.734929135157574E-4 102-103 0.0 0.0 2.578309711719191E-4 4.57662865378715 0.0012891548558595955 104-105 0.0 0.0 2.578309711719191E-4 4.823243977713091 0.0012891548558595955 106-107 0.0 0.0 2.578309711719191E-4 5.0708906255237185 0.0012891548558595955 108-109 0.0 0.0 2.578309711719191E-4 5.319955343675794 0.0012891548558595955 110-111 0.0 0.0 2.578309711719191E-4 5.600991102253185 0.0012891548558595955 112-113 0.0 0.0 2.578309711719191E-4 5.903426831437846 0.0012891548558595955 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTATG 100 4.403701E-4 29.78398 7 CCTTACG 80 0.005331407 29.780125 1 GTATACT 90 0.009468447 26.474648 3 ATGGGTA 330 1.8189894E-12 25.271257 5 GGTATCA 1350 0.0 25.147661 1 CATGGGT 540 0.0 23.165318 4 TATACTG 130 0.002036151 22.910753 5 TCTATAT 130 0.002036151 22.910753 3 GTATCAA 1760 0.0 21.996683 1 TGGGTAT 145 0.0038373414 20.540676 6 GTACATG 2855 0.0 19.61003 1 AACGCAG 1920 0.0 19.545736 6 ATCAACG 1945 0.0 18.988245 3 TATCAAC 2105 0.0 18.954966 2 ACATGGG 2800 0.0 18.72136 3 CAACGCA 2005 0.0 18.717115 5 GTTACTG 160 0.006779834 18.614988 3 TAACGTC 80 0.00684179 18.58852 46-47 TACATGG 2900 0.0 18.481825 2 GTTCTAC 195 9.825131E-4 18.326231 1 >>END_MODULE