FastQCFastQC Report
Sun 1 Jan 2017
ERR1142640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387851
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15450.3983488504606151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14730.37978502053623686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5930.15289376590494802No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4810.12401669713369311No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAGA2950.054.4647671
ATTACGT505.334928E-447.587833
GGTATCA14350.044.786361
GTGTTAT1700.042.005511
GTATCAA22000.039.4915431
TATAACG650.001939751636.606022
GTCAACG1156.635528E-736.222141
TCAACGC27050.032.5462074
ATCAACG26350.032.2820663
CAACGCA27100.031.8276545
GCGTCAA750.003904809531.7374951
GCGTATT750.003904809531.7374951
TATCAAC27750.030.8677832
AACGCAG29000.029.9436536
GTTAAGG800.0053544429.7539021
GTATAAC850.00719995728.0036741
ACGCAGA30750.027.4658577
GTATTAC900.00951523726.4479141
GTAAATG1358.246694E-526.4376833
CGCAGAG33500.025.388748
Produced by FastQC (version 0.11.5)