FastQCFastQC Report
Sun 1 Jan 2017
ERR1142638_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142638_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691538
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14470.20924374365544626No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12280.17757520194118037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23050.028.1665361
GTATCAA30850.027.995661
ATCAACG36200.022.8676243
TCAACGC36500.022.8428344
AACGCAG34950.022.8318396
CAACGCA35550.022.7831675
CATGGGG25200.022.4511154
TATCAAC37150.022.1225452
ACGCAGA39450.020.2274467
GTACATG53300.019.6681441
ACATGGG51750.019.5638073
TACATGG54350.019.2853682
CGCAGAG41150.019.247098
GCAGAGT43700.017.9877059
ATGGGGA9500.017.5529145
GTACATA2452.3077705E-417.0180511
CGGTATT1050.001628928716.99953382-83
CAGAGTA42950.016.49875810-11
ATGGGGG14100.016.472535
ATGGGTT2904.4780634E-516.4288375
Produced by FastQC (version 0.11.5)