##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142638_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	691538
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.425560128293746	33.0	33.0	33.0	27.0	33.0
2	31.50294127003867	33.0	33.0	33.0	27.0	33.0
3	31.612540453308423	33.0	33.0	33.0	27.0	33.0
4	31.56006755955566	33.0	33.0	33.0	27.0	33.0
5	31.57074087034986	33.0	33.0	33.0	27.0	33.0
6	34.8068753994719	37.0	37.0	37.0	27.0	37.0
7	34.9403546876672	37.0	37.0	37.0	33.0	37.0
8	34.9686756186934	37.0	37.0	37.0	33.0	37.0
9	34.91368948633336	37.0	37.0	37.0	33.0	37.0
10-11	34.955183229265785	37.0	37.0	37.0	33.0	37.0
12-13	34.97255682261857	37.0	37.0	37.0	33.0	37.0
14-15	34.94490469070391	37.0	37.0	37.0	33.0	37.0
16-17	34.92693604689836	37.0	37.0	37.0	30.0	37.0
18-19	34.94011102788278	37.0	37.0	37.0	27.0	37.0
20-21	35.02032715483459	37.0	37.0	37.0	33.0	37.0
22-23	35.0548617718766	37.0	37.0	37.0	33.0	37.0
24-25	35.09744511509129	37.0	37.0	37.0	33.0	37.0
26-27	35.0571132750478	37.0	37.0	37.0	33.0	37.0
28-29	35.097500065072346	37.0	37.0	37.0	33.0	37.0
30-31	35.14351488999881	37.0	37.0	37.0	33.0	37.0
32-33	35.14614092067247	37.0	37.0	37.0	33.0	37.0
34-35	35.142684856074425	37.0	37.0	37.0	33.0	37.0
36-37	35.084065517730046	37.0	37.0	37.0	33.0	37.0
38-39	35.07905277801075	37.0	37.0	37.0	33.0	37.0
40-41	35.061008216468224	37.0	37.0	37.0	33.0	37.0
42-43	35.04783901969234	37.0	37.0	37.0	33.0	37.0
44-45	35.06974670950837	37.0	37.0	37.0	33.0	37.0
46-47	35.0627933678265	37.0	37.0	37.0	33.0	37.0
48-49	35.03888651093649	37.0	37.0	37.0	33.0	37.0
50-51	35.021825987870514	37.0	37.0	37.0	30.0	37.0
52-53	34.99463370053417	37.0	37.0	37.0	30.0	37.0
54-55	34.951020768200735	37.0	37.0	37.0	27.0	37.0
56-57	34.88957945911866	37.0	37.0	37.0	27.0	37.0
58-59	34.843678872310704	37.0	37.0	37.0	27.0	37.0
60-61	34.816128687071426	37.0	37.0	37.0	27.0	37.0
62-63	34.853878601031326	37.0	37.0	37.0	27.0	37.0
64-65	34.791667269188395	37.0	37.0	37.0	27.0	37.0
66-67	34.761438272372594	37.0	37.0	37.0	27.0	37.0
68-69	34.736741871017934	37.0	37.0	37.0	27.0	37.0
70-71	34.6921976811108	37.0	37.0	37.0	27.0	37.0
72-73	34.6465552146086	37.0	37.0	37.0	27.0	37.0
74-75	34.57559888249091	37.0	37.0	37.0	27.0	37.0
76-77	34.51734105717979	37.0	37.0	37.0	27.0	37.0
78-79	34.44719596030876	37.0	37.0	37.0	27.0	37.0
80-81	34.38031026494567	37.0	37.0	37.0	27.0	37.0
82-83	34.35388510826593	37.0	37.0	37.0	27.0	37.0
84-85	34.3300888743641	37.0	37.0	37.0	27.0	37.0
86-87	34.25735679022701	37.0	37.0	37.0	27.0	37.0
88-89	34.192705245409506	37.0	37.0	37.0	27.0	37.0
90-91	34.13166955395076	37.0	37.0	37.0	27.0	37.0
92-93	34.00605245120297	37.0	37.0	37.0	27.0	37.0
94-95	33.923701951302746	37.0	37.0	37.0	24.5	37.0
96-97	33.84115695739063	37.0	37.0	37.0	22.0	37.0
98-99	33.805718557765445	37.0	35.0	37.0	22.0	37.0
100-101	33.68209122275276	37.0	33.0	37.0	22.0	37.0
102-103	33.61815330466294	37.0	33.0	37.0	22.0	37.0
104-105	33.493061841865526	37.0	33.0	37.0	22.0	37.0
106-107	33.412206559870896	37.0	33.0	37.0	22.0	37.0
108-109	33.295884680234494	37.0	33.0	37.0	22.0	37.0
110-111	33.2130150476185	37.0	33.0	37.0	22.0	37.0
112-113	33.12967761713745	37.0	33.0	37.0	14.0	37.0
114-115	32.94271464474838	37.0	33.0	37.0	14.0	37.0
116-117	32.76403755108179	37.0	33.0	37.0	14.0	37.0
118-119	32.65708970439802	37.0	33.0	37.0	14.0	37.0
120-121	32.58442992286758	37.0	33.0	37.0	14.0	37.0
122-123	32.381004369969546	37.0	33.0	37.0	14.0	37.0
124-125	30.735913283145685	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1230.0
1	111.0
2	37.0
3	37.0
4	28.0
5	17.0
6	18.0
7	17.0
8	22.0
9	21.0
10	22.0
11	21.0
12	20.0
13	14.0
14	39.0
15	162.0
16	324.0
17	627.0
18	1237.0
19	2268.0
20	3348.0
21	3989.0
22	4455.0
23	5054.0
24	5750.0
25	6721.0
26	8240.0
27	9959.0
28	11946.0
29	14825.0
30	18055.0
31	22612.0
32	28726.0
33	39422.0
34	60151.0
35	114928.0
36	327085.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.5986636247003	19.459296284040704	12.177589299709156	24.764450791549837
2	17.571544822764373	22.64774954198839	35.58028584566423	24.200419789583012
3	20.129228761013813	26.328003190833606	28.127778382102324	25.414989666050257
4	15.127442348890199	18.763099228514367	33.69473304044244	32.414725382152994
5	15.925408493531853	36.56617355843093	31.38425738538026	16.124160562656954
6	30.38893354497416	35.00982272662641	18.126340287107997	16.474903441291435
7	26.73675198792733	32.23779673805793	21.72050031922921	19.304950954785536
8	25.500821947224168	33.55953204868713	20.677812648266077	20.261833355822624
9	26.32489348610966	17.896510291505592	20.01982261228944	35.75877361009531
10-11	25.233761484929566	26.70133669199204	25.890571765211185	22.174330057867213
12-13	25.983873214503365	25.440533748135426	25.40926397780499	23.166329059556215
14-15	24.411303609113865	25.87318733599966	23.42031895776853	26.29519009711795
16-17	24.225735506854917	27.695867471355967	24.303395807213924	23.77500121457519
18-19	24.24604524054842	26.91926468338359	25.525736299097783	23.308953776970203
20-21	25.524045104780026	25.984981505996235	25.041939557738846	23.4490338314849
22-23	25.41158662566387	25.844087868941827	24.779508459364745	23.96481704602956
24-25	24.586732230899365	26.374188722352777	24.91167819404558	24.127400852702277
26-27	24.409747894286667	26.588816886298726	24.818495484132853	24.182939735281753
28-29	24.292740342174955	26.29779628660595	25.22348057128693	24.185982799932162
30-31	24.537231652340296	26.180657225274327	25.16945221563483	24.112658906750546
32-33	24.163054979779385	26.31738393077157	25.063126005595095	24.456435083853947
34-35	24.44081710756447	26.297741378772976	25.109973783148874	24.151467730513676
36-37	24.442328056133576	26.418034527570665	24.794503768548296	24.34513364774746
38-39	24.387598045183395	26.302413955025305	25.019855417775382	24.290132582015918
40-41	24.14656138423112	26.374293844688594	25.194867698712862	24.28427707236742
42-43	24.356061511969962	26.2835467293411	25.09390656378435	24.266485194904586
44-45	24.30488425244894	26.30606397456206	25.082210978951956	24.30684079403704
46-47	24.291846240029102	26.189894709074846	25.075531328199112	24.44272772269694
48-49	24.363993944835393	26.34721253004575	25.086178847649133	24.20261467746973
50-51	24.050454038518815	26.60033088179346	25.150712091533816	24.198502988153912
52-53	24.139928333704255	26.321669014222344	25.109735843804305	24.4286668082691
54-55	24.10424349964048	26.29746248231392	25.119888432723307	24.478405585322292
56-57	24.219411423601045	26.189765149318646	24.900768338648884	24.69005508843143
58-59	24.262802206882156	26.160555605486476	25.179316423180957	24.39732576445041
60-61	24.274704905424027	26.29910117849353	25.1665672325591	24.259626683523344
62-63	23.941680540345043	26.399876495874864	25.211294249302934	24.447148714477162
64-65	24.04683345752284	26.118575367567114	25.281673398027856	24.552917776882197
66-67	24.26614807652442	26.08894507109836	25.14913332337903	24.495773528998185
68-69	24.35132069591561	25.901367261816322	25.03469793769229	24.712614104575778
70-71	24.070216636588317	26.055894993948094	25.286614047675993	24.587274321787596
72-73	24.406308932435977	26.02521601614196	25.23290316127901	24.335571890143054
74-75	24.257489243622086	26.05322640608343	25.375413351460523	24.313870998833956
76-77	24.330925459801897	25.919730623527393	25.27201820001609	24.477325716654626
78-79	24.22570981884969	25.965167360908264	25.28008245372306	24.529040366518977
80-81	24.08468597874951	26.045946339649827	25.405648679146008	24.463719002454656
82-83	24.260174034060228	25.92832058219141	25.441083200748032	24.37042218300033
84-85	24.123462408635877	26.116551853081127	25.214904074010196	24.545081664272807
86-87	24.034492128765784	26.115251535298274	25.420481513016068	24.429774822919875
88-89	24.10480836681334	25.82663733180282	25.384078009867185	24.684476291516656
90-91	24.404843854388726	25.815799046127047	25.317124352744596	24.46223274673963
92-93	24.175140837567703	26.192272541801927	25.22262590947404	24.409960711156337
94-95	23.880489941673954	26.06715502380071	25.496213408541006	24.556141625984328
96-97	24.05199310777578	26.02676895832639	25.38971644207039	24.53152149182744
98-99	23.84292182390835	26.225149056752784	25.262955022500705	24.668974096838163
100-101	24.094246208548316	26.278777288527046	25.13645169095775	24.49052481196689
102-103	24.12199128095482	26.27494480860341	25.290302761127574	24.31276114931419
104-105	24.00313276807301	26.418783857115635	25.29123368319774	24.286849691613615
106-107	23.90628962259366	26.334398388651003	25.40468479434289	24.354627194412444
108-109	23.99513698248024	26.334993222060653	25.181548065407295	24.48832173005181
110-111	23.88141989525458	26.60090313765351	25.1018800998914	24.415796867200513
112-113	23.93463027577173	26.634170656218487	25.047454414782017	24.383744653227772
114-115	23.93081280322464	26.669424379387863	25.127085399504917	24.272677417882583
116-117	23.890434103461192	26.648925880657593	25.16056908654755	24.30007092933366
118-119	23.809623843162708	26.484909684111706	25.2969964244528	24.408470048272786
120-121	23.733959429327314	26.567690954690143	25.323274456935135	24.37507515904741
122-123	23.85442722991431	26.65900234927989	25.2421178146119	24.244452606193896
124-125	23.911441947796153	26.47208601689421	25.171517298780927	24.44495473652871
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1944.0
1	1232.5
2	420.0
3	267.0
4	191.5
5	157.5
6	142.0
7	141.5
8	141.5
9	142.5
10	133.5
11	123.0
12	113.5
13	111.0
14	137.5
15	163.5
16	169.0
17	180.5
18	218.0
19	246.5
20	293.5
21	364.0
22	437.5
23	580.0
24	763.0
25	968.0
26	1297.5
27	1760.5
28	2258.5
29	3019.5
30	4080.0
31	5078.5
32	6191.0
33	7388.5
34	8635.0
35	9985.0
36	11344.0
37	12944.5
38	14451.0
39	15556.0
40	16362.5
41	17179.0
42	18261.5
43	19828.5
44	21784.0
45	23546.5
46	25563.5
47	27252.0
48	28912.0
49	30176.5
50	30074.0
51	29298.5
52	28622.5
53	28672.0
54	28348.5
55	27076.5
56	25434.5
57	23411.5
58	21293.0
59	19103.5
60	16781.0
61	14290.5
62	11719.5
63	9324.0
64	7134.5
65	5548.5
66	4352.5
67	3437.5
68	2826.0
69	2339.0
70	1993.5
71	1759.0
72	1543.5
73	1318.5
74	1036.5
75	873.5
76	685.0
77	487.5
78	362.5
79	233.5
80	155.5
81	120.5
82	82.0
83	49.5
84	31.0
85	18.0
86	8.5
87	5.0
88	4.0
89	3.5
90	1.5
91	1.0
92	1.5
93	0.5
94	0.0
95	0.0
96	1.0
97	1.5
98	2.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3629590854009469
2	0.31017818254383706
3	0.29832055505265076
4	0.30208029059863667
5	0.3234818621680949
6	0.33548409487258835
7	0.343871197244403
8	0.3356287000858955
9	0.35081224748314627
10-11	0.34329277639117445
12-13	0.34329277639117445
14-15	0.33613481833247055
16-17	0.28870430836772526
18-19	0.2830647050487464
20-21	0.26824267068476354
22-23	0.26448293513877763
24-25	0.2503116242346769
26-27	0.25276991286089845
28-29	0.2396108384499478
30-31	0.24120149579632644
32-33	0.23830939153018346
34-35	0.24792563821510893
36-37	0.24344287660258726
38-39	0.22413808062608276
40-41	0.24741951996853392
42-43	0.23476656380415828
44-45	0.22341505455954697
46-47	0.2302838021916366
48-49	0.22392117280612198
50-51	0.22609025100572924
52-53	0.2622415543325168
54-55	0.2501670190213698
56-57	0.2513238607278269
58-59	0.24525044176892669
60-61	0.260506291772831
62-63	0.24329827138928015
64-65	0.25045622944798407
66-67	0.2485763616749911
68-69	0.23939393062998707
70-71	0.2419245218628622
72-73	0.2399000488765621
74-75	0.2311514334714795
76-77	0.23838169413683705
78-79	0.24532274437558022
80-81	0.2349111690174654
82-83	0.2502393216280233
84-85	0.22833163181199007
86-87	0.22059525290005758
88-89	0.22760860574545433
90-91	0.21820926688048956
92-93	0.23823708892352988
94-95	0.22233051545974333
96-97	0.21473874176111798
98-99	0.209460651475407
100-101	0.19767532659087425
102-103	0.20815920455564263
104-105	0.20439946900965675
106-107	0.20721927066914614
108-109	0.20700236284918544
110-111	0.20078433867697798
112-113	0.20273650905662452
114-115	0.19557855099792057
116-117	0.2045440742229639
118-119	0.18711914601945231
120-121	0.19304795976504543
122-123	0.19181881545193466
124-125	0.18820368511925592
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	691538.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.2953655106195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.81805661919215	70.51382954670314
2	8.540335221783446	13.714986764326392
3	1.8985766076429704	4.57340707981813
4	0.6789426922221313	2.180638065309603
5	0.34732495927093227	1.394429227781027
6	0.19571327582855683	0.9428921410760799
7	0.12791301358092574	0.718957552533169
8	0.07863100084678808	0.5050963962766944
9	0.055792061525908765	0.4031859575532435
>10	0.2398424915468792	3.2730924780651587
>50	0.0141540668199839	0.7468212608336279
>100	0.0042890815812790545	0.5720373929958196
>500	0.0	0.0
>1k	4.289081581041044E-4	0.4606261367279692
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1447	0.20924374365544626	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1228	0.17757520194118037	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	1.4460521330715014E-4
9	0.0	0.0	0.0	1.4460521330715014E-4	1.4460521330715014E-4
10-11	0.0	0.0	0.0	1.4460521330715014E-4	1.4460521330715014E-4
12-13	0.0	0.0	0.0	1.4460521330715014E-4	3.6151303326787537E-4
14-15	0.0	0.0	0.0	2.892104266143003E-4	4.3381563992145046E-4
16-17	0.0	0.0	0.0	2.892104266143003E-4	4.3381563992145046E-4
18-19	0.0	0.0	0.0	3.6151303326787537E-4	4.3381563992145046E-4
20-21	0.0	0.0	0.0	8.676312798429008E-4	4.3381563992145046E-4
22-23	0.0	0.0	0.0	0.0017352625596858018	4.3381563992145046E-4
24-25	0.0	0.0	0.0	0.004048945972600204	4.3381563992145046E-4
26-27	0.0	0.0	0.0	0.007013352845396783	4.3381563992145046E-4
28-29	0.0	0.0	0.0	0.015617363037172217	4.3381563992145046E-4
30-31	0.0	0.0	0.0	0.030150186974540805	4.3381563992145046E-4
32-33	0.0	0.0	0.0	0.05444386281014203	4.3381563992145046E-4
34-35	0.0	0.0	0.0	0.07996668295885404	4.3381563992145046E-4
36-37	0.0	0.0	0.0	0.11763634102536666	4.3381563992145046E-4
38-39	0.0	0.0	0.0	0.16882658653609778	5.061182465750255E-4
40-41	0.0	0.0	0.0	0.22789781617206864	5.784208532286006E-4
42-43	0.0	0.0	0.0	0.27482220789023887	5.784208532286006E-4
44-45	0.0	0.0	0.0	0.3280369263872701	5.784208532286006E-4
46-47	0.0	0.0	0.0	0.3913740098158019	5.784208532286006E-4
48-49	0.0	0.0	0.0	0.46020609135000534	5.784208532286006E-4
50-51	0.0	0.0	0.0	0.5249169243049551	5.784208532286006E-4
52-53	0.0	0.0	0.0	0.5766855906689148	5.784208532286006E-4
54-55	0.0	0.0	0.0	0.6340215577451999	7.230260665357507E-4
56-57	0.0	0.0	0.0	0.6921528534946741	7.230260665357507E-4
58-59	0.0	0.0	0.0	0.759249672469192	7.230260665357507E-4
60-61	0.0	0.0	0.0	0.8323476077959563	7.230260665357507E-4
62-63	0.0	0.0	0.0	0.901468899756774	7.230260665357507E-4
64-65	0.0	0.0	0.0	0.972253151670624	7.230260665357507E-4
66-67	0.0	0.0	0.0	1.0531597685159746	7.953286731893258E-4
68-69	0.0	0.0	0.0	1.142598092946447	8.676312798429009E-4
70-71	0.0	0.0	0.0	1.2340608903632195	8.676312798429009E-4
72-73	0.0	0.0	0.0	1.3221977678739274	0.0011568417064572011
74-75	0.0	0.0	0.0	1.405128857705578	0.0011568417064572011
76-77	0.0	0.0	0.0	1.4949286951693184	0.0012291443131107762
78-79	0.0	0.0	0.0	1.596224647090977	0.0013014469197643514
80-81	0.0	0.0	0.0	1.699689677212243	0.0013014469197643514
82-83	0.0	0.0	0.0	1.8094450341123698	0.0013014469197643514
84-85	0.0	0.0	0.0	1.9109578938539893	0.0013014469197643514
86-87	0.0	0.0	0.0	2.020857855967423	0.0013014469197643514
88-89	0.0	0.0	0.0	2.138349591779483	0.0013014469197643514
90-91	0.0	0.0	0.0	2.2703018489222573	0.0013014469197643514
92-93	0.0	0.0	0.0	2.4047123946912534	0.0013014469197643514
94-95	0.0	0.0	0.0	2.5474377402254103	0.0014460521330715015
96-97	0.0	0.0	0.0	2.6848849954738565	0.0014460521330715015
98-99	0.0	0.0	0.0	2.8307916557007715	0.0014460521330715015
100-101	0.0	0.0	0.0	2.997449164037262	0.0014460521330715015
102-103	0.0	0.0	0.0	3.1646127906203274	0.0014460521330715015
104-105	0.0	0.0	0.0	3.3492013454069047	0.0014460521330715015
106-107	0.0	0.0	0.0	3.520992338815799	0.0014460521330715015
108-109	0.0	0.0	0.0	3.694663199997686	0.0015183547397250765
110-111	0.0	0.0	0.0	3.8729614280054028	0.0015906573463786516
112-113	0.0	0.0	0.0	4.075987147488641	0.0015906573463786516
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2305	0.0	28.166536	1
GTATCAA	3085	0.0	27.99566	1
ATCAACG	3620	0.0	22.867624	3
TCAACGC	3650	0.0	22.842834	4
AACGCAG	3495	0.0	22.831839	6
CAACGCA	3555	0.0	22.783167	5
CATGGGG	2520	0.0	22.451115	4
TATCAAC	3715	0.0	22.122545	2
ACGCAGA	3945	0.0	20.227446	7
GTACATG	5330	0.0	19.668144	1
ACATGGG	5175	0.0	19.563807	3
TACATGG	5435	0.0	19.285368	2
CGCAGAG	4115	0.0	19.24709	8
GCAGAGT	4370	0.0	17.987705	9
ATGGGGA	950	0.0	17.552914	5
GTACATA	245	2.3077705E-4	17.018051	1
CGGTATT	105	0.0016289287	16.999533	82-83
CAGAGTA	4295	0.0	16.498758	10-11
ATGGGGG	1410	0.0	16.47253	5
ATGGGTT	290	4.4780634E-5	16.428837	5
>>END_MODULE