FastQCFastQC Report
Sun 1 Jan 2017
ERR1142638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691538
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26580.3843606569704051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26450.38248078919741213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14950.2161847938941895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26650.040.8532141
GTATCAA38150.035.8678781
ATCAACG46750.028.7565213
CAACGCA47250.028.5781145
TATCAAC48150.028.5381092
TCAACGC47950.028.2849754
AACGCAG50500.027.0883886
ACGCAGA53900.024.9382747
GTACTAG2151.231765E-724.9044191
CGCAGAG56450.024.0224698
GTACCGT1250.001636601523.7906726
CATGGGG24150.023.400014
ACATGGG47300.023.3916763
GAGTACT24300.023.25220112-13
GTACATG49850.023.0337411
GCAGAGT59800.022.3783479
TACATGG51100.022.1178152
CGGCGAA700.003163164221.24167398-99
CAGAGTA59500.020.69188710-11
GTCTAGC1450.0038681420.5151351
Produced by FastQC (version 0.11.5)