##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142638_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	691538
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.57976278960809	27.0	14.0	33.0	14.0	33.0
2	28.06229447984059	33.0	27.0	33.0	14.0	33.0
3	31.34111068372237	33.0	33.0	33.0	27.0	33.0
4	30.92807047479676	33.0	33.0	33.0	27.0	33.0
5	32.158922575476694	33.0	33.0	33.0	33.0	33.0
6	34.94793344689663	37.0	37.0	37.0	27.0	37.0
7	35.106793842131594	37.0	37.0	37.0	33.0	37.0
8	35.491797992301215	37.0	37.0	37.0	33.0	37.0
9	35.91191228826182	37.0	37.0	37.0	33.0	37.0
10-11	35.96942467369834	37.0	37.0	37.0	33.0	37.0
12-13	36.07070905720293	37.0	37.0	37.0	37.0	37.0
14-15	36.03984162837038	37.0	37.0	37.0	35.0	37.0
16-17	36.07915617073827	37.0	37.0	37.0	37.0	37.0
18-19	36.08604444007415	37.0	37.0	37.0	37.0	37.0
20-21	36.09799823003219	37.0	37.0	37.0	37.0	37.0
22-23	36.08844416358899	37.0	37.0	37.0	37.0	37.0
24-25	36.081994048049424	37.0	37.0	37.0	37.0	37.0
26-27	36.03844257293164	37.0	37.0	37.0	37.0	37.0
28-29	36.015408408503944	37.0	37.0	37.0	37.0	37.0
30-31	35.98675271640893	37.0	37.0	37.0	35.0	37.0
32-33	35.930444892399265	37.0	37.0	37.0	33.0	37.0
34-35	35.83566846651956	37.0	37.0	37.0	33.0	37.0
36-37	35.76246063123068	37.0	37.0	37.0	33.0	37.0
38-39	35.758988660059174	37.0	37.0	37.0	33.0	37.0
40-41	35.66627285846909	37.0	37.0	37.0	33.0	37.0
42-43	35.63741182697119	37.0	37.0	37.0	33.0	37.0
44-45	35.56398780688841	37.0	37.0	37.0	33.0	37.0
46-47	35.457459315323234	37.0	37.0	37.0	33.0	37.0
48-49	35.33335261402844	37.0	37.0	37.0	33.0	37.0
50-51	35.25499177196336	37.0	37.0	37.0	33.0	37.0
52-53	35.15933180823035	37.0	37.0	37.0	33.0	37.0
54-55	35.091488826355175	37.0	37.0	37.0	33.0	37.0
56-57	35.07689526822821	37.0	37.0	37.0	33.0	37.0
58-59	35.03451003415575	37.0	37.0	37.0	33.0	37.0
60-61	34.991586868689794	37.0	37.0	37.0	27.0	37.0
62-63	34.97246933646451	37.0	37.0	37.0	27.0	37.0
64-65	34.93840613241789	37.0	37.0	37.0	27.0	37.0
66-67	34.906173630371725	37.0	37.0	37.0	27.0	37.0
68-69	34.876273610416206	37.0	37.0	37.0	27.0	37.0
70-71	34.75247347217362	37.0	37.0	37.0	27.0	37.0
72-73	34.7608309304767	37.0	37.0	37.0	27.0	37.0
74-75	34.68238694041398	37.0	37.0	37.0	27.0	37.0
76-77	34.56248174359182	37.0	37.0	37.0	27.0	37.0
78-79	34.56771066810501	37.0	37.0	37.0	27.0	37.0
80-81	34.54240041761986	37.0	37.0	37.0	27.0	37.0
82-83	34.465179064635635	37.0	37.0	37.0	27.0	37.0
84-85	34.39231683580657	37.0	37.0	37.0	27.0	37.0
86-87	34.274240171906676	37.0	37.0	37.0	27.0	37.0
88-89	34.183106351350176	37.0	37.0	37.0	27.0	37.0
90-91	34.1226266669366	37.0	37.0	37.0	27.0	37.0
92-93	33.943978494312674	37.0	35.0	37.0	27.0	37.0
94-95	33.9854700681669	37.0	37.0	37.0	27.0	37.0
96-97	33.98676283877386	37.0	37.0	37.0	27.0	37.0
98-99	33.95751715740856	37.0	37.0	37.0	24.5	37.0
100-101	33.81644320341037	37.0	33.0	37.0	22.0	37.0
102-103	33.739814731800706	37.0	33.0	37.0	22.0	37.0
104-105	33.5887615720322	37.0	33.0	37.0	22.0	37.0
106-107	33.55523485332693	37.0	33.0	37.0	22.0	37.0
108-109	33.42541263097617	37.0	33.0	37.0	22.0	37.0
110-111	33.34810885302036	37.0	33.0	37.0	22.0	37.0
112-113	33.20931026205356	37.0	33.0	37.0	18.0	37.0
114-115	33.17246919185931	37.0	33.0	37.0	14.0	37.0
116-117	33.0257823865066	37.0	33.0	37.0	14.0	37.0
118-119	32.89031405360226	37.0	33.0	37.0	14.0	37.0
120-121	32.79738423629649	37.0	33.0	37.0	14.0	37.0
122-123	32.728794368494576	37.0	33.0	37.0	14.0	37.0
124-125	31.28643689862307	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	16.0
16	11.0
17	22.0
18	97.0
19	363.0
20	1299.0
21	2873.0
22	4699.0
23	6441.0
24	6003.0
25	5119.0
26	5276.0
27	6578.0
28	8676.0
29	11528.0
30	15580.0
31	21111.0
32	30454.0
33	45624.0
34	75387.0
35	152245.0
36	292135.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.91827194279151	14.65822733544895	12.477038211309205	28.946462510450328
2	17.751735985585753	20.482750044104588	37.304674508125366	24.460839462184293
3	20.556673034125435	25.398062576550494	28.505922315074972	25.5393420742491
4	15.046324748998959	18.427662678896535	33.436534298534696	33.0894782735698
5	16.953576686247118	34.45407559731947	32.20984344315161	16.382504273281796
6	30.697894357816008	34.011594697501025	18.417897886259748	16.87261305842322
7	26.666936596398173	31.285337899001934	22.60931431099954	19.438411193600352
8	26.194945180163636	31.672012239385257	21.219224395477905	20.913818184973206
9	26.595212410597828	16.084727086580923	20.77861231053102	36.541448192290225
10-11	25.643927014856736	24.98705783340901	26.575112285948133	22.793902865786116
12-13	26.70968674873018	23.823653814869044	26.48619922997668	22.98046020642409
14-15	24.800517108242786	24.20561126069717	25.054299257596835	25.93957237346321
16-17	23.910833533370546	26.279105414308397	25.933209744077697	23.876851308243367
18-19	23.72443741341763	25.10635713438741	27.96693746402945	23.20226798816551
20-21	24.96894589572473	24.173975286861836	27.853977022131925	23.00310179528151
22-23	24.817074405166455	24.025577770129768	27.748511289329002	23.408836535374775
24-25	24.01813060164445	24.616000856062865	27.788783841235045	23.577084701057643
26-27	23.854871315820677	24.742602720313272	27.84705974219783	23.555466221668222
28-29	23.680589648612752	24.621835190204916	28.127609700512124	23.569965460670208
30-31	23.915712391383252	24.412070503819752	28.153591203362943	23.51862590143405
32-33	23.711674140356916	24.58212648057877	27.859680682306227	23.84651869675809
34-35	23.866801245918516	24.7107895733857	27.824139815888643	23.59826936480714
36-37	23.94250715262328	24.668651156602863	27.6049684069877	23.783873283786157
38-39	23.797318440924432	24.677313466505094	27.762321087199837	23.763047005370638
40-41	23.706603906597397	24.713295662830927	27.87139970138354	23.70870072918813
42-43	23.896050560461354	24.6238585972957	27.720766389237756	23.75932445300519
44-45	23.780038117934225	24.822063285025553	27.509334266518977	23.888564330521245
46-47	23.915316294983064	24.774994288094074	27.418956008202006	23.890733408720852
48-49	23.963975949260924	24.991179081988264	27.231186138722673	23.81365883002814
50-51	23.791028114145572	25.22970538133841	27.064962446026104	23.914304058489915
52-53	23.79283567931191	25.089293719217164	27.047320610002636	24.070549991468294
54-55	23.833758954677833	25.112936671592884	26.931058018503684	24.1222463552256
56-57	23.926957014654292	24.95871521160081	26.83272647345482	24.281601300290077
58-59	23.86521058857214	25.00361513033268	26.999094771364696	24.132079509730485
60-61	23.822552050646532	25.091101284383505	27.129890186801013	23.95645647816895
62-63	23.49979321454497	25.216907819960728	27.188961416436985	24.094337549057318
64-65	23.58272430437662	24.951340345722144	27.216508709571997	24.249426640329236
66-67	23.76818049044304	24.894293589072472	27.167342937047568	24.17018298343692
68-69	23.787846799452815	24.832980978630243	27.163004780648354	24.216167441268592
70-71	23.59971541694021	24.869783005416913	27.404712394691256	24.125789182951625
72-73	23.78452087954675	24.975850929377703	27.44433422313741	23.795293967938132
74-75	23.73553496569777	24.899394121809447	27.438381160617777	23.92668975187501
76-77	23.707270547080284	24.990176305465884	27.220498703561784	24.08205444389205
78-79	23.665310991811726	24.92504021835403	27.36445962800282	24.045189161831427
80-81	23.52893116502636	25.133398309275844	27.360029383779345	23.977641141918447
82-83	23.56848069086587	25.050105706410932	27.460313099207852	23.92110050351535
84-85	23.52752204628925	25.087910211476515	27.25093553829991	24.133632203934326
86-87	23.422228240758507	25.079820032891142	27.35837511106734	24.139576615283012
88-89	23.566651502024044	24.9174879238252	27.1994285730844	24.316432001066353
90-91	23.715488813646648	24.9304765081876	27.12150672946877	24.232527948696983
92-93	23.56002331213281	25.151981350293756	27.09868923551408	24.189306102059362
94-95	23.255812272019032	25.149794116690106	27.302982867761095	24.291410743529767
96-97	23.493864400799378	25.015472757823865	27.11022387779124	24.380438963585515
98-99	23.417585150779857	25.18451625217992	26.89317145261721	24.50472714442301
100-101	23.526834389433407	25.355222706489016	26.65204225942754	24.46590064465004
102-103	23.5266897842201	25.201941180383436	27.005312795536906	24.26605623985956
104-105	23.530083763841642	25.409835472920456	26.74304347983269	24.317037283405217
106-107	23.478463945582163	25.392386246308952	26.75876090684821	24.370388901260668
108-109	23.562147975156716	25.420260724330657	26.543125076821855	24.474466223690776
110-111	23.404353773762253	25.630695637839136	26.422192272875822	24.542758315522793
112-113	23.3973573378161	25.659562930426766	26.391410444119085	24.551669287638052
114-115	23.373263652901212	25.694755747334202	26.455740682363082	24.4762399174015
116-117	23.49062340255851	25.682701755730548	26.362565623890603	24.464109217820344
118-119	23.35932613921877	25.524212352909277	26.53970319758509	24.576758310286863
120-121	23.317930434619143	25.516946683219178	26.575820654441234	24.58930222772045
122-123	23.262423756901285	25.77804835019912	26.51351046507929	24.44601742782031
124-125	23.327226111338465	25.637606990865198	26.509296040235363	24.525870857560978
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	204.0
1	217.0
2	378.5
3	471.5
4	328.5
5	289.5
6	450.5
7	928.0
8	1489.0
9	1747.5
10	1547.5
11	1201.0
12	1075.5
13	1002.0
14	959.0
15	976.0
16	945.0
17	911.0
18	919.5
19	897.5
20	892.0
21	869.5
22	888.5
23	950.5
24	1014.5
25	1178.0
26	1420.0
27	1698.0
28	2070.5
29	2782.0
30	3694.0
31	4487.5
32	5609.5
33	6829.5
34	8003.0
35	9359.0
36	10699.0
37	12106.0
38	13540.0
39	14639.5
40	15460.5
41	16387.0
42	17633.5
43	19332.5
44	21159.5
45	22869.5
46	24943.5
47	26819.5
48	28599.5
49	29947.5
50	30259.0
51	29463.0
52	28497.5
53	28339.5
54	27868.0
55	26912.0
56	25650.5
57	23470.0
58	21403.5
59	19520.5
60	16989.0
61	14237.5
62	11599.5
63	9117.5
64	6996.5
65	5504.0
66	4351.5
67	3437.5
68	2667.5
69	2207.5
70	1866.0
71	1623.5
72	1448.0
73	1323.0
74	1084.5
75	847.5
76	668.0
77	456.0
78	325.0
79	236.0
80	158.0
81	106.5
82	75.5
83	53.5
84	32.0
85	15.5
86	6.0
87	1.5
88	0.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024872096688829823
2	0.0
3	1.4460521330715014E-4
4	0.001012236493150051
5	0.004627366825828805
6	0.0021690781996072522
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	7.230260665357507E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	4.3381563992145046E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0010845390998036261
30-31	1.4460521330715014E-4
32-33	1.4460521330715014E-4
34-35	0.0
36-37	0.0012291443131107762
38-39	0.0
40-41	0.002241380806260827
42-43	0.0013737495264179265
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.06868747632089632
76-77	0.08770306187078657
78-79	7.230260665357507E-5
80-81	0.0
82-83	0.0
84-85	0.07114576494711787
86-87	0.15595672255176143
88-89	0.1931925649783526
90-91	0.08452174717802927
92-93	0.13166304671616022
94-95	7.953286731893259E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0015183547397250765
106-107	0.0
108-109	4.3381563992145046E-4
110-111	0.0
112-113	7.230260665357507E-5
114-115	0.0
116-117	3.6151303326787537E-4
118-119	7.230260665357507E-5
120-121	2.1690781996072523E-4
122-123	0.0
124-125	0.001012236493150051
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	691538.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.15679538413866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.05879173787484	68.04236172247424
2	9.096294311510416	14.21874426517248
3	1.991843745681345	4.6702837220517965
4	0.7139574605751907	2.232025086366177
5	0.3541102213841779	1.3838060058077617
6	0.21039263690336168	0.9866168563671252
7	0.14013450435947028	0.7666724648436563
8	0.0904894539660818	0.565789259043959
9	0.059967446401116044	0.4218177094273196
>10	0.2604073016881278	3.507260571432394
>50	0.015303567011595973	0.8138447131423528
>100	0.00699588433206881	0.9394293436415149
>500	6.558641561057513E-4	0.2897912271331246
>1k	6.558641561057513E-4	1.1615570530960972
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2658	0.3843606569704051	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2645	0.38248078919741213	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1495	0.2161847938941895	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	4.3381563992145046E-4
9	0.0	0.0	0.0	1.4460521330715014E-4	4.3381563992145046E-4
10-11	0.0	0.0	0.0	1.4460521330715014E-4	4.3381563992145046E-4
12-13	0.0	0.0	0.0	1.4460521330715014E-4	5.784208532286006E-4
14-15	0.0	0.0	0.0	2.892104266143003E-4	7.230260665357507E-4
16-17	0.0	0.0	0.0	2.892104266143003E-4	8.676312798429009E-4
18-19	0.0	0.0	0.0	3.6151303326787537E-4	8.676312798429009E-4
20-21	0.0	0.0	0.0	8.676312798429008E-4	8.676312798429009E-4
22-23	0.0	0.0	0.0	0.0017352625596858018	8.676312798429009E-4
24-25	0.0	0.0	0.0	0.004265853792560929	8.676312798429009E-4
26-27	0.0	0.0	0.0	0.007664076305278957	8.676312798429009E-4
28-29	0.0	0.0	0.0	0.016051178677093665	0.001012236493150051
30-31	0.0	0.0	0.0	0.030656305221115832	0.0011568417064572011
32-33	0.0	0.0	0.0	0.05444386281014203	0.0011568417064572011
34-35	0.0	0.0	0.0	0.07924365689231828	0.0011568417064572011
36-37	0.0	0.0	0.0	0.1169133149588309	0.0011568417064572011
38-39	0.0	0.0	0.0	0.16889888914275136	0.0011568417064572011
40-41	0.0	0.0	0.0	0.22833163181199007	0.0011568417064572011
42-43	0.0	0.0	0.0	0.2756898391700817	0.0012291443131107762
44-45	0.0	0.0	0.0	0.3289768602737666	0.0013014469197643514
46-47	0.0	0.0	0.0	0.39224164109564474	0.0013737495264179265
48-49	0.0	0.0	0.0	0.4615798408764233	0.0014460521330715015
50-51	0.0	0.0	0.0	0.5269413972912551	0.0015906573463786516
52-53	0.0	0.0	0.0	0.5792884845084435	0.0015906573463786516
54-55	0.0	0.0	0.0	0.6376366880778785	0.0017352625596858018
56-57	0.0	0.0	0.0	0.6968525229271565	0.0017352625596858018
58-59	0.0	0.0	0.0	0.7638770392950207	0.0017352625596858018
60-61	0.0	0.0	0.0	0.8366857641951708	0.0017352625596858018
62-63	0.0	0.0	0.0	0.9056624509426814	0.0017352625596858018
64-65	0.0	0.0	0.0	0.9761574924299171	0.0017352625596858018
66-67	0.0	0.0	0.0	1.0577148327351498	0.0018075651663393769
68-69	0.0	0.0	0.0	1.147876183232158	0.001879867772992952
70-71	0.0	0.0	0.0	1.2399897041088126	0.001879867772992952
72-73	0.0	0.0	0.0	1.3286326998660956	0.001879867772992952
74-75	0.0	0.0	0.0	1.411419184484439	0.001879867772992952
76-77	0.0	0.0	0.0	1.5016528375881006	0.001879867772992952
78-79	0.0	0.0	0.0	1.6027318816897986	0.001879867772992952
80-81	0.0	0.0	0.0	1.7066307274509862	0.002096775592953677
82-83	0.0	0.0	0.0	1.8157353608912308	0.0024582886262215524
84-85	0.0	0.0	0.0	1.916814404992929	0.0024582886262215524
86-87	0.0	0.0	0.0	2.0260636436464807	0.0024582886262215524
88-89	0.0	0.0	0.0	2.1438445898851546	0.0024582886262215524
90-91	0.0	0.0	0.0	2.2765198730944647	0.0024582886262215524
92-93	0.0	0.0	0.0	2.4111473266834214	0.002675196446182278
94-95	0.0	0.0	0.0	2.5543787904641535	0.002747499052835853
96-97	0.0	0.0	0.0	2.6917537431059464	0.002747499052835853
98-99	0.0	0.0	0.0	2.8379496137594753	0.002747499052835853
100-101	0.0	0.0	0.0	3.0043179116693515	0.002747499052835853
102-103	0.0	0.0	0.0	3.1716261434657245	0.002892104266143003
104-105	0.0	0.0	0.0	3.35578088261238	0.002892104266143003
106-107	0.0	0.0	0.0	3.5272103629880065	0.002892104266143003
108-109	0.0	0.0	0.0	3.7010981319898546	0.003036709479450153
110-111	0.0	0.0	0.0	3.879540965210878	0.003036709479450153
112-113	0.0	0.0	0.0	4.084518855073763	0.003036709479450153
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2665	0.0	40.853214	1
GTATCAA	3815	0.0	35.867878	1
ATCAACG	4675	0.0	28.756521	3
CAACGCA	4725	0.0	28.578114	5
TATCAAC	4815	0.0	28.538109	2
TCAACGC	4795	0.0	28.284975	4
AACGCAG	5050	0.0	27.088388	6
ACGCAGA	5390	0.0	24.938274	7
GTACTAG	215	1.231765E-7	24.904419	1
CGCAGAG	5645	0.0	24.022469	8
GTACCGT	125	0.0016366015	23.790672	6
CATGGGG	2415	0.0	23.40001	4
ACATGGG	4730	0.0	23.391676	3
GAGTACT	2430	0.0	23.252201	12-13
GTACATG	4985	0.0	23.033741	1
GCAGAGT	5980	0.0	22.378347	9
TACATGG	5110	0.0	22.117815	2
CGGCGAA	70	0.0031631642	21.241673	98-99
CAGAGTA	5950	0.0	20.691887	10-11
GTCTAGC	145	0.00386814	20.515135	1
>>END_MODULE